A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\RpA-30

General Information
SymbolDmel\RpA-30SpeciesD. melanogaster
NameReplication Protein A 30Annotation symbol
Feature typegeneFlyBase IDFBgn0010174
Created / Updated2006-05-31/2006-05-31
Genomic Location
Chromosome (arm)Recombination map
Cytogenetic mapSequence location
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Automatically generated summary

See sections below for more information
The gene Replication Protein A 30 is referred to in FlyBase by the symbol RpA-30 (FBgn0010174). It has not been localized to the genome sequence. Its molecular function is described as single-stranded DNA binding. It is involved in the biological process DNA-dependent DNA replication. No alleles are reported. It has no annotated transcripts.
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FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
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Comments on Gene Model
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Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
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Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Additional Polypeptide Data & Comments
Reported size (kD)
30 (kD)
(Mitsis et al., 1993)
Comments
RpA-30 protein, the 30kD subunit of single-stranded DNA-binding protein or SSB (a heterotrimer) was purified from 0-18hr embryos. SSB was characterized biochemically and was shown to stimulate the extent and processivity of DNA synthesis of its cognate DNA polymerase α. It is the Drosophila cognate of yeast and human SSB/RP-A proteins.
(Mitsis et al., 1993)
External Data
Linkouts
Crossreferences
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Maps to
      Does NOT map to
      Identified with
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      Type
      Symbol & Location
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      FlyBase-Curated Data
      Transcript and
      Protein data       		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
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      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
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        Stage
        Tissue/Position
        Reference
         embryonic stage
         
        (Mitsis et al., 1993)
        Marker for
          Subcellular Localization
          CV Term
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          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
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          Phenotype manifest in
          Allele
          hide Classical Alleles ( 0 )
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          Allele of RpA-30ClassMutagenStocksKnown lesion
          hide Alleles Carried on Transgenic Constructs ( 0 )
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          Allele of RpA-30ClassMutagenStocksKnown lesion
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          Transgenic Constructs
          Type of construct
          Name
          Expression data
          Insertions
          Type of insertions
          Name
          Expression data
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          Please look at the allele reports for the complete phenotype data
          Drosophila single stranded binding protein is a trimer with subunits of 70kD, 30kD and 8kD representing the cognates of the human and yeast SSB/RP-A proteins.
          (Mitsis et al., 1993)
          Replication protein A has been purified and characterised. The protein consists of three subunits (encoded by RpA-30, RpA-70 and RpA-8), and binds single-stranded DNA very tightly via the large subunit (encoded by RpA-70).
          (Marton et al., 1994)
          RpA-30, RpA-70 and RpA-8 can substitute for human RP-A for the initiation of SV40 DNA replication.
          (Kamakaka et al., 1994)
          The Replication Protein A complex from ovaries and early embryos has multiple phosphorylated forms. The relative abundance of these forms is tightly regulated during development. The localization of the Replication Protein A complex varies during the cell cycle in both ovaries and embryos.
          (Mitsis, 1995)
          hide Gene Ontology: Function, Process & Cellular Component ( 4 )
          hide Molecular Function
          CV term
          Evidence
          References
          single-stranded DNA binding
          inferred from direct assay
          (Mitsis et al., 1993)
          hide Biological Process
          CV term
          Evidence
          References
          DNA-dependent DNA replication
          inferred from direct assay
          (Mitsis et al., 1993)
          hide Cellular Component
          CV term
          Evidence
          References
          DNA replication factor A complex
          inferred from direct assay
          (Mitsis et al., 1993)
          nucleus
          inferred from direct assay
          (Mitsis, 1995)
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          gene
           
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          Interacts with
          Please look at the allele data for full details of the genetic interactions
          RpA-30 allele
          Gene
          References
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          Genome-wide drosophilid orthologs
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          Produces phenotype in
          Produces NO phenotype in
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          cDNA Clones, Fully Sequenced
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          Source for database identity of
          Source for database merge of
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          Sequence Crossreferences
          Other Crossreferences
          Linkouts
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
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          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
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          hide Synonyms & Secondary IDs ( 13 )
          Reported As
          Symbol Synonym
          DmRPA
          (Takeuchi et al., 2004)
          DmRP-A
          (Dusenbery and Smith, 1996)
          dRP-A
          (Thommes et al., 1995, Thommes et al., 1995, Marton et al., 1994)
          DRP-A
          (Mitsis, 1995)
          D-SSB
          (Mitsis et al., 1993)
          RPA
          (Murawsky et al., 2003, Plank et al., 2006)
          RP-A
          (Oshige et al., 1999, Kamakaka et al., 1994)
          RPA30
          (Takata et al., 2004, Takata et al., 2002, Shimanouchi et al., 2003, Leclerc et al., 1996)
          RpA-30
          (Cayirlioglu et al., 2003)
          Ssb-30
           
          Name Synonym
          Drosophila Replication Protein A
          (Mitsis, 1995)
          Replication Protein A 30
           
          Single stranded-binding protein 30
           
          Secondary FlyBase IDs
            hide References ( 16 )
            Research paper
            Plank et al., 2006, Proc. Natl. Acad. Sci. USA 103(30): 11118--11123
            Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration. [FBrf0191828]
            Takata et al., 2004, Genetics 168(2): 855--865
            Drosophila damaged DNA-binding protein 1 is an essential factor for development. [FBrf0180301]
            Takeuchi et al., 2004, Biochem. J. 382(2): 535--543
            Purification of Drosophila DNA polymerase z by REV1 protein-affinity chromatography. [FBrf0179970]
            Cayirlioglu et al., 2003, Molec. Cell. Biol. 23(6): 2123--2134
            Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. [FBrf0159210]
            Takata et al., 2002, Nucleic Acids Res. 30(17): 3795--3808
            Drosophila damage-specific DNA-binding protein 1 (D-DDB1) is controlled by the DRE/DREF system. [FBrf0152283]
            Oshige et al., 1999, Mutat. Res. 433(3): 183--192
            A new DNA polymerase species from Drosophila melanogaster: a probable mus308 gene product. [FBrf0108977]
            Leclerc et al., 1996, Molec. Biol. Cell 7(4): 505--513
            Drosophila Cdk8, a kinase partner of cyclin C that interacts with the large subunit of RNA polymerase II. [FBrf0087556]
            Mitsis, 1995, Dev. Biol. 170(2): 445--456
            Phosphorylation and localization of replication protein A during oogenesis and early embryogenesis of Drosophila melanogaster. [FBrf0083305]
            Thommes et al., 1995, Nucleic Acids Res. 23(21): 4443--4450
            Purification and characterisation of a DNA helicase, dhel I, from Drosophila melanogaster embryos. [FBrf0084443]
            Thommes et al., 1995, J. Biol. Chem. 270(36): 21137--21143
            Mitochondrial single-stranded DNA-binding protein from Drosophila embryos. Physical and biochemical characterization. [FBrf0083479]
            Kamakaka et al., 1994, Molec. Cell. Biol. 14(8): 5114--5122
            Simian virus 40 origin- and T-antigen-dependent DNA replication with Drosophila factors in vitro. [FBrf0073501]
            Marton et al., 1994, FEBS Lett. 342(2): 139--144
            Purification and characterisation of dRP-A: a single-stranded DNA binding protein from Drosophila melanogaster. [FBrf0068586]
            Mitsis et al., 1993, Biochemistry 32(19): 5257--5266
            A single-stranded DNA binding protein from Drosophila melanogaster: characterization of the heterotrimeric protein and its interaction with single-stranded DNA. [FBrf0057831]
            Review
            Dusenbery and Smith, 1996, Mutat. Res. 364(3): 133--145
            Cellular responses to DNA damage in Drosophila melanogaster. [FBrf0090538]
            Abstract
            Murawsky et al., 2003, A. Dros. Res. Conf. 44 Suppl.: 1028B
            Replication Protein A function in scs enhancer blocking and its recruitment to the borders of the 87A7 heat shock-induced puff. [FBrf0157109]
            Shimanouchi et al., 2003, A. Dros. Res. Conf. 44 Suppl.: 1018A
            Expression of Replication Protein A during development of D. melanogaster. [FBrf0157099]