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Gene Dmel\Con

General Information
Symbol	Dmel\Con	Species	D. melanogaster
Name	Connectin	Annotation symbol	CG7503
Feature type	protein_coding_gene	FlyBase ID	FBgn0005775
Created / Updated	2003-12-01/2008-02-01
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64C2-64C4	Sequence location	3L:4,938,048..4,976,172 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Connectin is referred to in FlyBase by the symbol Con (CG7503, FBgn0005775). It has the cytological map location 64C2-64C4. Its sequence location is 3L:4938048..4976172. Its molecular function is described as protein binding. It is involved in the biological processes: cell adhesion; axonal fasciculation; homophilic cell adhesion; synaptic target attraction. 12 alleles are reported. The phenotypes of these alleles are annotated with 37 unique terms, many of which group under: hypodermal muscle of larval abdomen; abdominal oblique muscle; organ system; larval abdominal segment 5; abdominal ventral oblique muscle 4; hypodermal muscle of larval abdominal 2; abdominal 7 longitudinal muscle; embryonic neuron; nervous system; abdominal segment 7. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64C2-64C4   Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64C3-64C5 (determined by in situ hybridisation)   (Nose et al., 1994) 64C-64C (determined by in situ hybridisation)   (Gould and White, 1992) 64C-64C (determined by in situ hybridisation)   (Nose et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Con for information on other features
Comments on Gene Model
	DGC clone GH14524 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Con-RA FBtr0073375  NM_079206   3763   691
Additional Transcript Data & Comments
Reported size (kB)	5 (northern blot) (Gould and White, 1992) 5.0 (northern blot) (Nose et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Con-PA   FBpp0073231   77.2   691   7.05     NP_523930   AAF47952
Additional Polypeptide Data & Comments
Reported size (kD)	682 (aa); 76 (kD) (Nose et al., 1992) 682 (aa); 72 (kD predicted) (Gould and White, 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0005775
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cysteine-rich flanking region, C-terminal (IPR000483) Leucine-rich repeat (IPR001611) Leucine-rich repeat, typical subtype (IPR003591)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698569   AE003483 AAF47952   AI108939   AI134790   AI238476   AW940556   AY119518 AAM50172   BG632682   BH900960   BI166453   BI171371   BI214728   BI374660   BI486635   BP555729   BP555730   BT015228   CO265429   CO275347   CO280371   CO283430   CO311125   CO319903   CO331217   CO339547   EC053663   EC055739   EC060679   EC061814   EC062033   EC078578   EC213505   EC233815   EC241226   EC244637   EC257658   GH12337   M96647 AAA28424   X68701 CAA48652
UniProtKB/Swiss-Prot	Q01819
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	GH14524 RE20842 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2418
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage  abdominal 1..7 dorsal transverse muscle 1 (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 2 (Nose et al., 1992)  embryonic stage  neuron (Nose et al., 1992)  embryonic stage  muscle fibre <of> larval muscle system (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 1 (Nose et al., 1992)  embryonic stage  larval sense organ <of> embryonic gnathal segment (Gould and White, 1992)  embryonic stage | stage 11-14  somatic mesoderm (Gould and White, 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 3 (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral oblique muscle 1 (Nose et al., 1992)  embryonic stage | stage 11-17  embryonic central nervous system | presumptive (Gould and White, 1992)  embryonic stage  embryonic central nervous system | restricted (Nose et al., 1992)  embryonic stage | stage 10  visceral mesoderm | presumptive (Gould and White, 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 4 (Nose et al., 1992)  embryonic stage  abdominal 1..7 ventral acute muscle 2 (Nose et al., 1992)  embryonic stage  abdominal 1 ventral acute muscle 3 (Nose et al., 1992)  embryonic stage  visceral mesoderm (Nose et al., 1992)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage  myoblast (Nose et al., 1992)  embryonic stage  anterior commissure (Nose et al., 1992)  embryonic stage  RP1 neuron (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 3 (Nose et al., 1992)  embryonic stage  interface glial cell (Nose et al., 1992)  embryonic stage  muscle fibre <of> larval muscle system (Nose et al., 1992)  embryonic stage  visceral mesoderm (Nose et al., 1992)  embryonic stage  abdominal 1 ventral acute muscle 3 (Nose et al., 1992)  embryonic stage  abdominal 1..7 dorsal transverse muscle 1 (Nose et al., 1992)  embryonic stage  peripheral glial cell 4 (Nose et al., 1992)  embryonic stage | late  larval muscle system (Broadie and Bate, 1993)  embryonic stage  abdominal 1..7 lateral transverse muscle 1 (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 2 (Nose et al., 1992)  embryonic stage  abdominal 1..7 ventral acute muscle 2 (Nose et al., 1992)  embryonic stage  peripheral glial cell 3 (Nose et al., 1992)  embryonic stage  posterior commissure (Nose et al., 1992)  embryonic stage  growth cone (Nose et al., 1992)  embryonic stage  longitudinal connectives (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral oblique muscle 1 (Nose et al., 1992)  embryonic stage  motor neuron (Nose et al., 1992)  embryonic stage  abdominal 1..7 lateral transverse muscle 4 (Nose et al., 1992)  embryonic stage  midline glial cell (Nose et al., 1992)  embryonic stage  VUM neuron (Nose et al., 1992)  embryonic stage  peripheral glial cell 1 (Nose et al., 1992)
Marker for
Subcellular Localization
CV Term	neuromuscular junction (Broadie and Bate, 1993) plasma membrane axon (Nose et al., 1992)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI238476
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005775 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0005775 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0005775
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable ConFvex236 ConFvex238 ConTl.6.5LR viable, with Scer\GAL4how-24B ConScer\UAS.cRa Other Phenotypes Allele neuroanatomy defective, with Scer\GAL4how-24B ConScer\UAS.cRa Sterility Allele male fertile ConTl.6.5LR female fertile ConTl.6.5LR Phenotype manifest in Allele abdominal posterior fascicle & embryo ConFvex238 ConMhc.PN ConTl.6.5LR abdominal ventral muscle & embryo ConMhc.PN abdominal 1 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 2 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 3 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 4 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 5 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 6 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 7 ventral oblique muscle 4, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 1 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 2 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 3 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 4 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 5 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 6 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 7 ventral oblique muscle 5, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 2 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 3 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 4 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 5 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 6 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 7 ventral oblique muscle 6, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 1 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 2 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 3 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 4 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 5 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 6 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 7 ventral longitudinal muscle 1, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 1 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 2 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 3 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 4 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 5 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 6 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal 7 ventral longitudinal muscle 2, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal posterior fascicle, with Scer\GAL4how-24B ConScer\UAS.cRa RP3 neuron, with Scer\GAL4how-24B ConScer\UAS.cRa abdominal posterior fascicle ConTl.6.5LR RP1 neuron ConTl.6.5LR RP3 neuron ConTl.6.5LR
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Con Class Mutagen Stocks Known lesion ConFvex236 hypomorph PM hybrid dysgenesis 0 Yes ConFvex238 loss of function, hypomorph PM hybrid dysgenesis 0 Yes ConKG07829 P-element activity 1 -- ConMB00185 1 -- ConrF400 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Con Class Mutagen Stocks Known lesion ConF9.35 in vitro construct | other 0 Yes ConGD2312 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes ConMhc.PN in vitro construct | regulatory fusion 0 Yes ConScer\UAS.cCa in vitro construct | regulatory fusion 0 Yes ConScer\UAS.cRa in vitro construct | regulatory fusion 0 Yes ConTl.6.5LR in vitro construct | regulatory fusion 0 Yes Cona.F9.35 in vitro construct | other 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)10H   Df(3L)GN24
Useful duplication	Dp(3;3)M34
Disrupted in	Df(3L)10H (Nose et al., 1994, Nose et al., 1997) Df(3L)FX-GJ1 (Nose et al., 1994) Df(3L)FX-OI1 (Nose et al., 1994) Df(3L)Flex14 (Nose et al., 1994)
Not disrupted in	Df(3L)X37 (Nose et al., 1994)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{lacZCon.1-35} Yes P{lacZCon.1} Yes P{lacZCon.4-35} Yes P{lacZCon.4} Yes UAS construct P{GD2312} NA P{UAS-Con.C} NA P{UAS-Con.R} NA characterization construct P{Mhc'-con} NA P{Toll-connectin} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}ConKG07829 NA insertion of enhancer trap binary system Mi{ET1}ConMB00185 No insertion of enhancer trap P{PZ}ConrF400 Yes
Related Comments
	Please look at the allele reports for the complete phenotype data Cloned as a potential Ubx target gene.   The genes encoding the 35 and 48 transcripts, Con and T48 respectively, are good candidates for the target genes directly regulated by the homeotic gene Ubx. (Gould et al., 1990) Con is a novel cell adhesion molecule whose expression suggests a role in target recognition. (Nose et al., 1992) A chromatin immunopurification approach isolated the Connectin gene as a binding site for Ubx in vivo. The product is predicted to be a cell surface molecule containing leucine rich repeats and can mediate cell-cell adhesion. (Gould and White, 1992) In pros mutants, where innervation of muscles fails, connectin is expressed on muscle surface begins in same developmental pattern as in wild type. (Broadie and Bate, 1993) The pattern of expression of Con protein during embryogenesis has been analysed. (Meadows et al., 1994) Con functions as a repulsive signal for SNb growth cones in vivo, Con has a specific and robust repulsive function during motoneuron growth cone guidance and synapse formation. (Nose et al., 1994) Con gene product plays a role in neuromuscular connectivity. (Nose et al., 1993) Ectopic expression of Con in all muscles causes SNa motorneurons to often inappropriately innervate a neighbouring non-target muscle that ectopically expresses Con. The ectopic synapse formation is dependent on the endogenous Con expression on the SNa motoneurons. Results show that Con can function as an attractive and homophilic target recognition molecule in vivo. Con has a specific function during SNb pathfinding. (Nose et al., 1997)
Gene Ontology: Function, Process & Cellular Component ( 6 )
Molecular Function
CV term Evidence References
protein binding inferred from electronic annotation with InterPro:IPR001611 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
axonal fasciculation traceable author statement (Chiba, 1999)
cell adhesion non-traceable author statement (FlyBase, 1992-)
homophilic cell adhesion traceable author statement (Chiba, 1999) non-traceable author statement (Swiss-Prot Project Members, 1993.10.1)
synaptic target attraction traceable author statement (Chiba, 1999)
Cellular Component
CV term Evidence References
plasma membrane traceable author statement (Chiba, 1999)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Con allele Gene References ConFvex238 Sema-1a (Yu et al., 2000) unspecified Fas2 (Yu et al., 2000)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 64058
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24709 Dere\GG14154 Dgri\GH15784 Dmoj\GI12564 Dpse\GA28329 Dsec\GM13940 Dsim\GD13223 Dvir\GJ15467 Dwil\GK17589 Dyak\GE20580
Curated drosophilid orthologs	Dpse\GA20397
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000011314 Anopheles gambiae (Ensembl)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v17898 w1118; P{GD2312}v17898/CyO
Bloomington	22723 y1 w67c23; Mi{ET1}ConMB00185 14662 y1; P{SUPor-P}ConKG07829 ry506
Genomic Clones ( 2 )
	BACR15L14 BACR17L24
cDNA Clones ( 18 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE20842
Other clones
cDNA Clones, End Sequenced (