Gene Dmel\spi

General Information
Symbol	Dmel\spi	Species	D. melanogaster
Name	spitz	Annotation symbol	CG10334
Feature type	protein_coding_gene	FlyBase ID	FBgn0005672
Created / Updated	2005-03-18/2005-03-18
Genomic Location
Chromosome (arm)	2L	Recombination map	2-54
Cytogenetic map	37F2-37F2	Sequence location	2L:19,567,899..19,577,324 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene spitz is referred to in FlyBase by the symbol spi (CG10334, FBgn0005672). It has the cytological map location 37F2. Its sequence location is 2L:19567899..19577324. Its molecular function is described as: epidermal growth factor receptor binding; gurken receptor binding; epidermal growth factor receptor activating ligand activity. It is involved in the biological processes described with 17 unique terms, many of which group under: anatomical structure development; regulation of developmental process; open tracheal system development; organ morphogenesis; regulation of apoptosis; cell cycle; sensory organ development; programmed cell death; cell motility; positive regulation of signal transduction; oenocyte development. 66 alleles are reported. The phenotypes of these alleles are annotated with 120 unique terms, many of which group under: peripheral nervous system; nervous system; organ system; embryonic nervous system; adult segment; hypodermal muscle of larval abdomen; embryonic/larval tracheal system; commissure; portion of tissue; adult mesothoracic segment. It has 7 annotated transcripts and 7 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spi: spitz Embryonic lethal. Normal allele required for normal development of blastoderm cells just lateral to the ventral mesodermal precursors. Denticle bands narrower than normal; first row often missing; reversal of polarity between anterior and posterior rows not seen; irregular fusion in midline of adjacent dentical bands. Keilin's organs missing or strongly reduced. Labrum, antennal-maxillary complex and labial sense organ reduced in size; right and left halves of head skeleton fused to produce pointed appearance. Tail region normal. Right and left halves of ventral ganglia of the central nervous system closer together than normal resulting in shorter commissures. The cells in the CNS that stain with anti-eve+ antibodies are closer together in spi than in wild type. spi+ required in the female germ line; spi pole cells transplanted into normal embryos produce no progeny.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 37F2-37F2 Limits computationally determined from genome sequence between P{EP}EP623&P{PZ}spi⁰¹⁰⁶⁸ and P{lacW}l(2)k10239^k10239
Experimentally Determined Cytological Location
Cytogenetic map Notes References 37F1-37F2 (determined by in situ hybridisation) (Spradling et al., 1999) 37F-37F (Calvi et al., 1998) 37F1-37F2 (determined by in situ hybridisation) (Perrimon et al., 1996) 38A-38A (determined by in situ hybridisation) (Kania et al., 1995) 37F-38A (determined by in situ hybridisation) (Tio et al., 1994) 37F1-37F2 (BDGP Project Members, 1994-1999) 37F-38A (determined by in situ hybridisation) (Rutledge et al., 1992)
Experimentally Determined Recombination Data
Location	2-54 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\spi for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spi-RA FBtr0081268 NM_134291 1663 234 spi-RB FBtr0081269 NM_134293 1635 234 spi-RC FBtr0081271 NM_134294 1563 234 spi-RD FBtr0081272 NM_134295 1938 234 spi-RE FBtr0081270 NM_134292 1439 234 spi-RF FBtr0081267 NM_057561 1467 234 spi-RG FBtr0100597 NM_001032110 1577 234
Additional Transcript Data & Comments
Reported size (kB)	2.5, 2.0 (northern blot) (Rutledge et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein spi-PA FBpp0080809 26.4 234 7.54 NP_599118 AAF53831 spi-PB FBpp0080810 26.4 234 7.54 NP_599120 AAG22442 spi-PC FBpp0080812 26.4 234 7.54 NP_599121 AAN11050 spi-PD FBpp0080813 26.4 234 7.54 NP_599122 AAN11051 spi-PE FBpp0080811 26.4 234 7.54 NP_599119 AAN11052 spi-PF FBpp0080808 26.4 234 7.54 NP_476909 AAN11053 spi-PG FBpp0100054 26.4 234 7.54 NP_001027281 AAZ66477
Additional Polypeptide Data & Comments
Reported size (kD)	230 (aa); 26 (kD) (Rutledge et al., 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0005672
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • EGF-like, type 3 (IPR000742) EGF-like (IPR006209) EGF (IPR006210) EGF-like region, conserved site (IPR013032)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201448 AA247046 AA438721 AA978455 AC005128 AE003663 AAF53831 AE003663 AAG22442 AE003663 AAN11050 AE003663 AAN11051 AE003663 AAN11052 AE003663 AAN11053 AI062056 AI063044 AI108506 AI114351 AI134122 AI134774 AI294122 AI387575 AI403295 AI514252 AI532159 AI532794 AQ073391 AQ073742 AQ073946 AQ254855 AX094034 AY089693 AAL90431 BG633450 BG635682 BG635952 BG640183 BG640811 BI161727 BI161728 BI166071 BI168272 BI170426 BI173403 BI229299 BI229516 BI236311 BI357251 BI361709 BI362492 BI363234 BI369323 BI370205 BI482146 BI483575 BI573448 BI604260 BI612280 BI613693 BI617993 BI623152 BI628817 BI633012 BI635844 BI637027 BI637197 BI639961 BI641057 BP544654 BP544655 CL943613 CO180297 CO183986 CO268259 CO274052 CO286995 CO287880 CO290956 CO295405 CO295624 CO298405 CO298754 CO299844 CO304067 CO304230 CO309942 CO309945 CO312308 CO316357 CO333899 CO341027 CO341270 CZ473487 CZ473488 CZ475768 CZ485801 EC062427 EC064807 EC073742 EC075506 EC077956 EC078379 EC078724 EC081814 EC082420 EC083468 EC087657 EC196331 EC202202 EC212984 EC223719 EC225039 EC225553 EC230131 EC241981 EC244146 EC246647 EC251511 EC253459 EC260333 G00577 M95199 AAA28894 RE01152 RH09080

UniProtKB/Swiss-Prot	Q01083
UniProtKB/TrEMBL

Maps to	DS08491 (FlyBase, 1992-)
Does NOT map to
Identified with	RH69567 (BDGP Project Members, 2000-) Dm0293 (BDGP Project Members, 1994-1999, Spradling et al., 1999) D1320 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References aberration junction Tp(2;2)dpp[d13].bk3 2L:19,566,791..19,569,748 comment=Mapped to a 2.9 kb BamHI-EcoRI fragment; position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=EcoRI-BamHI_rfrag (Rutledge et al., 1992) rescue fragment spi[+t12.5] 2L:19,566,791..19,578,810 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=BamHI-BamHI_rfrag (Rutledge et al., 1992)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 8098
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage peripheral nervous system (Rutledge et al., 1992) embryonic stage ventral midline (Rutledge et al., 1992) embryonic stage mesoderm (Rutledge et al., 1992)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference embryonic stage ventral midline (Rutledge et al., 1992) embryonic stage mesoderm (Rutledge et al., 1992)
Marker for

Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005672 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0005672 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0005672 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for spi Stage(s) 4-6 Stage(s) 7-8 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable spi^EY14184 spi^SCP1 spi^SCP2 viable \| poor spi^SCP1 spi^SCP2 lethal \| recessive spi⁰¹⁰⁶⁸ spi⁰⁷¹³² spi¹⁰⁶⁸¹ spi^133a spi^SC1 spi^SC2 spi^SC3 spi^k05808 spi^s3547 lethal \| male \| pharate adult stage, with Scer\GAL4^71B spi^s.Scer\UAS lethal \| female \| pupal stage, with Scer\GAL4^71B spi^s.Scer\UAS lethal \| embryonic stage spi² spi^37Fa-2 spi^37Fa-3 lethal \| embryonic stage \| recessive spi^s3547 spi^unspecified lethal \| larval stage, with Scer\GAL4^hs.PB spi^s.Scer\UAS lethal \| pupal stage, with Scer\GAL4^GMR.PF spi^s.Scer\UAS lethal, with Scer\GAL4^Kr.PM spi^Scer\UAS.cSa lethal, with Scer\GAL4^h-H10 spi^s.Scer\UAS lethal, with Scer\GAL4^cb41 spi^s.Scer\UAS Other Phenotypes Allele wild-type spi^+t12.5 spi^s.sev visible spi^SCP1 spi^SCP2 spi^s.sev visible \| recessive spi^SCP1 visible \| dominant spi^s3547 visible \| homeotic, with Scer\GAL4^ey.PB spi^s.Scer\UAS visible \| female, with Scer\GAL4^71B spi^s.Scer\UAS visible, with Scer\GAL4^GMR.PF spi^s.Scer\UAS visible, with Scer\GAL4^bs-1348 spi^s.Scer\UAS visible, with Scer\GAL4^kni.L2 spi^s.Scer\UAS reduced cell number, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} spi^s.Scer\UAS increased cell number, with Scer\GAL4^{nos.UTR.T:Hsim\VP16} spi^{RNAi.Scer\UAS} cell death increase spi¹ cell death increase \| somatic clone