A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\Ets65A

General Information
SymbolDmel\Ets65ASpeciesD. melanogaster
NameEts at 65AAnnotation symbolCG7018
Feature typeprotein_coding_geneFlyBase IDFBgn0005658
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map 65A6-65A6 Sequence location3L:6,091,222..6,117,030 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Ets at 65A is referred to in FlyBase by the symbol Ets65A (CG7018, FBgn0005658). It has the cytological map location 65A6. Its sequence location is 3L:6091222..6117030. Its molecular function is described as: DNA binding; transcription factor activity; sequence-specific DNA binding. It is involved in the biological process regulation of transcription, DNA-dependent. 2 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
65A6-65A6  
Limits computationally determined from genome sequence between P{PZ}loj04026 and P{PZ}l(3)0209402094  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
65A-65A
(determined by in situ hybridisation)  
(Chen et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Ets65A for information on other features
detailed view FBtr0077051 FBtr0077052 FBtr0077053 FBtr0077054 FBtr0273355 FBtr0077055 FBtr0077062 FBtr0100355 FBpp0076760 FBpp0076761 FBpp0076759 FBpp0271863 FBpp0076762 FBpp0076763 FBpp0099761 FBpp0076770 FBti0078151 FBti0052513 FBti0033622 FBti0018659 FBti0003333 FBti0003334
Comments on Gene Model
3' UTR contains 2 non-overlapping ORFs that are conserved among close Drosophila species (potential peptides 127 aa and 61 aa in length).
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Ets65A-RA
FBtr0077054
 NM_168156
  3702
  490
Ets65A-RB
FBtr0077055
 NM_079221
  3497
  257
Ets65A-RC
FBtr0273355
 
  3357
  512
Additional Transcript Data & Comments
Reported size (kB)
0.8 (northern blot)
(Chen et al., 1992)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Ets65A-PA  
FBpp0076762  
52.1  
490  
8.45  
  NP_729142
 
Ets65A-PB  
FBpp0076763  
28.7  
257  
9.25  
  NP_523945
 
Ets65A-PC  
FBpp0271863  
54.5  
512  
8.52  
 
 
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Ets (IPR000418)
Winged helix repressor DNA-binding (IPR011991)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003564
AAF50696
 
AE003564
AAF50697
 
AI109004
 
AI387796
 
AI387800
 
AI532488
 
AW940263
 
AW940704
 
AY060250
AAL25289
 
AY060316
AAL25355
 
BG639657
 
BI370904
 
BI564078
 
BI569798
 
BI573889
 
BI573890
 
BI576296
 
BI576341
 
BI584176
 
BI585889
 
BI589716
 
BI612085
 
BI612860
 
BI634128
 
BT003453
AAO39456
 
CO270440
 
CO272757
 
CO272929
 
CO290932
 
CO341977
 
EC059440
 
EC077587
 
EC083296
 
EC200577
 
EC208407
 
EC220735
 
EC250904
 
EC252436
 
EC257189
 
M88473
AAA28450
 
RH09362
 
SD04025
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage
     ventral nerve cord
    (Chen et al., 1992)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0077051 FBtr0077052 FBtr0077053 FBtr0077054 FBtr0273355 FBtr0077055 FBtr0077062 FBtr0100355
        AI387796
        AI109004
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Ets65A
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for Ets65A
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 1 )
        For All Classical Alleles Show

        Allele of Ets65AClassMutagenStocksKnown lesion
        Ets65AMB03856
          		1 --
          hide Alleles Carried on Transgenic Constructs ( 1 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of Ets65AClassMutagenStocksKnown lesion
          Ets65AGD47522 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
          Useful duplication
           
          Disrupted in
          (Ryder, 2004.4.26)
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          UAS construct
          P{GD4752}
          NA
          Insertions
          Type of insertions
          Name
          Expression data
          insertion of enhancer trap binary system
          Mi{ET1}Ets65AMB03856
          No
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          Ets65A has been cloned, sequenced and its RNA expression pattern characterised.
          (Chen et al., 1992)
          hide Gene Ontology: Function, Process & Cellular Component ( 5 )
          hide Molecular Function
          CV term
          Evidence
          References
          DNA binding
          inferred from sequence or structural similarity with UniProt:P27577
          (Chen et al., 1992)
          non-traceable author statement
          (Swiss-Prot Project Members, 1993.4.1)
          sequence-specific DNA binding
          inferred from electronic annotation with InterPro:IPR000418
          (FlyBase Curators et al., 2004-)
          transcription factor activity
          inferred from sequence or structural similarity with UniProt:P27577
          (Chen et al., 1992)
          inferred from sequence or structural similarity with MGD:Ets1; MGI:MGI:95455
          (FlyBase, 1992-)
          hide Biological Process
          CV term
          Evidence
          References
          regulation of transcription, DNA-dependent
          inferred from sequence or structural similarity with UniProt:P27577
          (Chen et al., 1992)
          hide Cellular Component
          CV term
          Evidence
          References
          nucleus
          inferred from sequence or structural similarity with UniProt:P27577
          (Chen et al., 1992)
          inferred from sequence or structural similarity with MGD:Ets1; MGI:MGI:95455
          (FlyBase, 1992-)
          non-traceable author statement
          (Swiss-Prot Project Members, 1993.4.1)
          hide Sequence Ontology: Class of Gene
          protein_coding_gene
           
          nuclear_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          Ets65A allele
          Gene
          References
          hide External Data
          Linkouts
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
          Linkouts
          InParanoid orthologs - Eukaryotic orthologs
          •
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 3 )
          VDRC
          v15355
          w1118 P{GD4752}v15355
          v15354
          w1118; P{GD4752}v15354/TM3
          Bloomington
          24537
          w1118; Mi{ET1}Ets65AMB03856
          hide Genomic Clones ( 1 )
          hide cDNA Clones ( 8 )
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          hide Antibody Information
          hide Other Information
          hide Discoverer
          hide Etymology
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for identity of: Ets65A CG7018
          (Gabrielian, 1999.11)
          Source for database merge of
          Source for merge of: Ets65A CG6882 CG13294 Annotations CG7018, CG6882, CG13294 merged as CG7018 in release 3 of the genome annotation.
          (FlyBase Genome Annotators, 2002-2003)
          Additional comments
          hide Comments About Role
          hide Comments About Molecular Function
          hide Other Comments
          dsRNA made from templates generated with primers directed against this gene was tested in an RNAi screen to identify genes that regulate Ca[2+] release.
          (Zhang et al., 2006)
          hide External Crossreferences & Linkouts
          Sequence Crossreferences
          RefSeq (Transcripts)
          •
          RefSeq (Proteins)
          •
          DDBJ / EMBL / GenBank
          •
          UniProtKB/Swiss-Prot
          •
          Other Crossreferences
          InterPro domains - A database of protein families, domains, and functional sites
          •
          Ets (IPR000418)
          Winged helix repressor DNA-binding (IPR011991)
          Linkouts
          BDGP expression data
          •
          Show all BDGP original images, annotations and embryonic microarray data for Ets65A
          BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
          •
          DEDB - Drosophila exon database: splicing graphs
          •
          DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
          •
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          •
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          •
          FlyExpress - Embryonic expression images (BDGP data)
          •
          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
          •
          GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
          •
          GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
          •
          InParanoid orthologs - Eukaryotic orthologs
          •
          PANTHER - Protein classification by function, families, and pathways
          •
          University of Chicago Dev. Expression
          •
          hide Synonyms & Secondary IDs ( 11 )
          Reported As
          Symbol Synonym
          CG6882
           
          CG7018
           
          CG13294
           
          D-ets-3
          (Hsu and Schulz, 2000, Chen, 1999.9.28)
          D-Ets-3
          (Chen et al., 1992)
          Ets
          (Ringrose et al., 2004, Guruharsha et al., 2004)
          ETS3
          (Wasylyk et al., 1993)
          Ets3
           
          ets65A
           
          Ets65A
          (Zhang et al., 2006)
          Name Synonym
          Ets at 65A
           
          Secondary FlyBase IDs
          • FBgn0035681
          • FBgn0040840
          hide References ( 21 )
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          List References by type
          hide Recent research papers ( 1 )
          Zhang et al., 2006, Proc. Natl. Acad. Sci. USA 103(24): 9357--9362
          Genome-wide RNAi screen of Ca(2+) influx identifies genes that regulate Ca(2+) release-activated Ca(2+) channel activity. [FBrf0194902]
          Show all research papers ...
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          All reviews listed in FlyBase were published before 2006
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