• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\mxc

General Information
Symbol	Dmel\mxc	Species	D. melanogaster
Name	multi sex combs	Annotation symbol	CG12058
Feature type	protein_coding_gene	FlyBase ID	FBgn0005639
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map	1-27.7
Cytogenetic map	8D2-8D2	Sequence location	X:9,137,858..9,139,961 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene multi sex combs is referred to in FlyBase by the symbol mxc (CG12058, FBgn0005639). It has the cytological map location 8D2. Its sequence location is X:9137858..9139961. Its molecular function is described as: mRNA binding; RNA binding; nucleotide binding. It is involved in the biological processes: hemocyte differentiation; hemocyte proliferation; hemopoiesis; RNA processing. 11 alleles are reported. The phenotypes of these alleles are annotated with 47 unique terms, many of which group under: organ system; adult segment; embryonic/larval hemocoel; thoracic segment; imaginal precursor; nervous system; adult; peripheral nervous system; anatomical structure; adult mesothoracic segment. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 8D2-8D2   Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912EP912
Experimentally Determined Cytological Location
Cytogenetic map Notes References 8D4-8D9   (Remillieux, 1999.12.20)
Experimentally Determined Recombination Data
Location	1-27.7
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\mxc for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mxc-RA FBtr0071312  NM_080056   2044   595
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein mxc-PA   FBpp0071247   67.4   595   7.42     NP_524795   AAF46473
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0005639
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif, RNP-1 (IPR000504) Lupus La protein (IPR002344) RNA-binding protein Lupus La (IPR006630) Nucleotide-binding, alpha-beta plait (IPR012677) Winged helix repressor DNA-binding (IPR011991) RNA binding motif (IPR014886)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264871   AA697190   AA942170   AE003446 AAF46473   AF219009 AAF27644   AW942074   AY061107 AAL28655   BI234268   CK658506   CO189545   CO189621   CO195247   EC057905   EC218136   EC229844
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KJ96 Q9W362
Maps to
Does NOT map to
Identified with	LD09531 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 970
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005639 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0005639 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0005639
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable mxcG46 mxcG9 mxcM1 lethal | recessive mxc16a-1 mxc1 mxcG48 mxcM1 lethal | embryonic stage | non-rescuable maternal effect | recessive mxcM1 lethal | embryonic stage | partially | recessive | rescuable maternal effect mxcM1 lethal | larval stage | recessive mxc16a-1 mxc1 mxc22a-6 mxcG48 mxcSO lethal | pupal stage | recessive mxc16a-1 mxc1 mxcSO lethal | pharate adult stage | recessive mxcG43 cell lethal | cell autonomous | somatic clone mxc22a-6 Other Phenotypes Allele visible mxcM1 visible | recessive mxc1 mxcG43 mxcG46 mxcSO visible | dominant | homeotic mxcG43 visible | homeotic mxcG43 mxcG46 visible | homeotic | recessive mxcM1 Sterility Allele sterile | recessive mxcG43 female sterile mxcG46 female sterile | recessive mxc1 mxcG46 mxcM1 mxcSO male sterile mxcG46 male sterile | recessive mxcG46 female fertile mxcG9 Phenotype manifest in Allele imaginal disc mxc1 mxc16a-1 mxc22a-6 mxcG48 lymph gland mxc1 mxc16a-1 mxcG43 mxcG46 crystal cell mxc1 mxc16a-1 larval hemocyte mxc1 mxc16a-1 mxcG43 podocyte mxc1 mxc16a-1 mxcG43 lamellocyte mxc1 mxc16a-1 mxcG43 eye mxc1 mxc22a-6 mxcG43 mxcG46 mxcSO adult mesothoracic segment mxc1 mxcG43 mxcSO leg mxc1 mxcG43 mxcSO wing mxc1 mxcG43 mxcSO adult abdominal segment 4 mxc1 mxcG43 mxcG46 mxcM1 mxcSO eye-antennal disc mxc1 mxc16a-1 ventral thoracic disc mxc1 mxc16a-1 dorsal mesothoracic disc mxc1 mxc16a-1 dorsal metathoracic disc mxc1 mxc16a-1 genital disc mxc1 mxc16a-1 melanotic mass mxc1 mxc16a-1 mxc22a-6 mxcG48 mxcM1 mxcSO egg chamber mxc1 mxc16a-1 mxc22a-6 mxcG43 mxcG46 mxcG48 mxcG9 mxcM1 mxcSO anterior lymph gland pair mxc1 embryonic/larval fat body mxc1 larval midgut mxc1 imaginal disc & larva mxc1 larval central nervous system mxc1 gonad & larva mxc1 prohemocyte mxc1 plasmatocyte mxc1 embryonic/larval hemolymph mxc1 ventral prothoracic disc mxc16a-1 egg mxc22a-6 mxcG46 mxcG48 mxcG9 mxcM1 chorion mxc22a-6 mxcG46 mxcG48 mxcG9 mxcM1 dorsal appendage mxc22a-6 mxcG46 mxcG48 mxcG9 mxcM1 hindgut imaginal ring mxc22a-6 larval hindgut & embryo mxc22a-6 adult head mxc22a-6 mxcSO antennal segment 3 mxc22a-6 mxcSO arista mxc22a-6 mxcSO maxillary palpus mxc22a-6 mxcSO larval digestive system mxc22a-6 unguis mxcG43 sex comb mxcG43 mesothoracic leg mxcG43 metathoracic leg mxcG43 antenna mxcG43 gonad | female mxcG43 testis mxcG43 mxcG46 mxcM1 male accessory gland mxcG43 mxcG46 mxcM1 pigment cell mxcG48 embryonic head mxcM1 embryonic/first instar larval cuticle | dorsal mxcM1 embryonic segment mxcM1 sex comb | ectopic mxcM1
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of mxc Class Mutagen Stocks Known lesion mxc16a-1 γ ray 1 -- mxc1 ethyl methanesulfonate 3 -- mxc22a-6 γ ray 1 -- mxc2 0 -- mxcG43 ethyl methanesulfonate 1 -- mxcG46 hypomorph ethyl methanesulfonate 1 -- mxcG48 amorph ethyl methanesulfonate 1 -- mxcG9 hypomorph ethyl methanesulfonate 0 -- mxcM1 hypomorph ethyl methanesulfonate 0 -- mxcSO 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mxc Class Mutagen Stocks Known lesion mxcGD7468 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)10-70d   Df(1)9a4-5
Duplicated in	Dp(1;4)A17 (Docquier et al., 1996) Dp(1;4)mxc+R (Umea Stock Center, 2001.6) Dp(1;Y)FF1 (Santamaria and Randsholt, 1995, Docquier et al., 1996) Dp(1;Y)lz+ (Santamaria and Randsholt, 1995)
Disrupted in	Df(1)10-70d (Santamaria and Randsholt, 1995, Docquier et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7468} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data A Pc-group gene. Besides the common homeotic transformations characteristic of Pc-group mutants, mxc mutants zygotically mimic the maternal effect mutants of the posterior group. Loss of normal mxc function can promote uncontrolled malignant growth which indicates a possible relationship between Pc-group genes and tumor suppressor genes. (Santamaria and Randsholt, 1995)
Gene Ontology: Function, Process & Cellular Component ( 9 )
Molecular Function
CV term Evidence References
RNA binding inferred from electronic annotation with InterPro:IPR002344 (FlyBase Curators et al., 2004-)
mRNA binding inferred from sequence or structural similarity (Lasko, 2000)
nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
RNA processing inferred from electronic annotation with InterPro:IPR002344 (FlyBase Curators et al., 2004-)
hemocyte differentiation inferred from mutant phenotype (Remillieux-Leschelle et al., 2002)
hemocyte proliferation inferred from mutant phenotype (Remillieux-Leschelle et al., 2002)
hemopoiesis traceable author statement (Evans et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from electronic annotation with InterPro:IPR002344 (FlyBase Curators et al., 2004-)
ribonucleoprotein complex inferred from electronic annotation with InterPro:IPR002344 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mxc allele Gene References mxcG43 dom (Ruhf et al., 2001) dpp (Saget et al., 1998) tub (Remillieux-Leschelle et al., 2002) Tl (Remillieux-Leschelle et al., 2002) z (Santamaria and Randsholt, 1995) mxcG46 Dfd (Saget et al., 1998) z (Santamaria and Randsholt, 1995) hh (Randsholt et al., 2000) mxcG48 z (Santamaria and Randsholt, 1995) mxcM1 E(z) (Docquier et al., 1996, Saget et al., 1998) Pc (Docquier et al., 1996, Saget et al., 1998) corto (Lopez et al., 2001) Dfd (Saget et al., 1998) Scm (Docquier et al., 1996, Saget et al., 1998) Pcl (Docquier et al., 1996, Saget et al., 1998) Psc (Docquier et al., 1996, Saget et al., 1998) pho (Saget et al., 1998) Asx (Saget et al., 1998) sxc (Saget et al., 1998) unspecified ash1 (Gildea et al., 2000) trx (Gildea et al., 2000)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 69370
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19208 Dere\GG18304 Dgri\GH12028 Dmoj\GI14301 Dper\GL18292 Dpse\GA11366 Dsec\GM22404 Dsim\GD24774 Dvir\GJ19358 Dwil\GK25140 Dyak\mxc
Curated drosophilid orthologs	Dpse\GA11366   Dyak\mxc
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000026312 Anopheles gambiae (Ensembl) ENSBTAP00000012693 Bos taurus (Ensembl) ENSCAFP00000017237 Canis familiaris (Ensembl) ENSGALP00000019653 Gallus gallus (Ensembl) ENSMMUP00000023546 Macaca mulatta (Ensembl) ENSMODP00000007024 Monodelphis domestica (Ensembl) ENSRNOP00000036555 Rattus norvegicus (Ensembl) GSTENP00013801001 Tetraodon nigroviridis (Ensembl) ENSXETP00000051994 Xenopus tropicalis (Ensembl) Q7XQH8 Oryza sativa (Gramene) AT5G46250.1 Arabidopsis thaliana (Arabidopsis) ZDB-CDNA-040425-987 Danio rerio (Zfin) ZDB-GENE-030131-6896 Danio rerio (Zfin) MGI:107634 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Kyoto	101105 FM7 / mxc1 101107 FM7 / y1 mxcSO 108753 w1 mxc1; Dp(1;4)mxc+R
VDRC	v31635 w1118; P{GD7468}v31635 v31636 w1118; P{GD7468}v31636
Bloomington	7129 w1 mxcG43/FM7c 7140 w1 mxcG46/FM7a 7141 y1 ac1 mxcG48/FM7a 6360 w1 mxc1; Dp(1;4)mxc+R 7128 y1 cv1 mxc22a-6 v1 f1 car1/FM7c 7133 y1 cv1 mxc16a-1 v1 f1 car1/FM7a
Genomic Clones ( 1 )
	BACR30C13
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD09531
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP15461 GM09405 LD26238 RE29944
Other clones	EK044734 EK044520 EC39923
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0005639 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0005639
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mxc CG12058 (Lasko, 2000.6.8)
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	Mutants display a blood cell neoplastic phenotype. (Mechler, 1994) Candidate gene for cyst presence/absence quantitative trait locus. (Macdonald and Goldstein, 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080056 RefSeq (Proteins) • NP_524795 DDBJ / EMBL / GenBank • AA264871 AE003446 AAF46473 AF219009 AAF27644 AW942074 AY061107 AAL28655 UniProtKB/TrEMBL • Q7KJ96 Q9W362
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif, RNP-1 (IPR000504) Lupus La protein (IPR002344) RNA-binding protein Lupus La (IPR006630) Nucleotide-binding, alpha-beta plait (IPR012677) Winged helix repressor DNA-binding (IPR011991) RNA binding motif (IPR014886)
Linkouts
	BioGRID -