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Gene Dmel\faf

General Information
Symbol	Dmel\faf	Species	D. melanogaster
Name	fat facets	Annotation symbol	CG1945
Feature type	protein_coding_gene	FlyBase ID	FBgn0005632
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	100D2-100D2	Sequence location	3R:27,602,756..27,615,753 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene fat facets is referred to in FlyBase by the symbol faf (CG1945, FBgn0005632). It has the cytological map location 100D2. Its sequence location is 3R:27602756..27615753. Its molecular function is described as: ubiquitin-specific protease activity; ubiquitin thiolesterase activity. It is involved in the biological processes described with 11 unique terms, many of which group under: cellular macromolecule metabolic process; biopolymer modification; anatomical structure development; protein modification process; sensory organ development; multicellular organismal development; embryonic development; transport; cell motility; ubiquitin cycle; catabolic process; compound eye development; regulation of metabolic process; cellular localization. 53 alleles are reported. The phenotypes of these alleles are annotated with 22 unique terms, many of which group under: peripheral nervous system; nervous system; organ system; adult segment; adult mesothoracic segment; blastoderm embryo; cytoplasmic part; synapse; syncytial blastoderm embryo; neuromuscular junction. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 100D2-100D2   Limits computationally determined from genome sequence between P{EP}pygoEP1076 and P{PZ}ttk02667&P{lacW}ttkj6C7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 100D1-100D3 (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000) 100E-100E   (Fischer-Vize, 1993.1.22) 100E-100E   (Fischer-Vize, 1993.1.22) 100E-100E (determined by in situ hybridisation)   (Fischer-Vize et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\faf for information on other features
Comments on Gene Model
	DGC clone LD22582 appears problematic: incomplete CDS DGC clone appears problematic: BcDNA:RE01547 appears 3' truncated and incomplete.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) faf-RA FBtr0085843  NM_079873   9090   2778 faf-RC FBtr0085844  NM_170576   8668   2711
Additional Transcript Data & Comments
Reported size (kB)	8.9, 8.5 (compiled cDNA) (Fischer-Vize et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein faf-PA   FBpp0085202   311.1   2778   5.95     NP_524612   AAF57198 faf-PC   FBpp0085203   304.1   2711   6.02     NP_733455   AAN14291
Additional Polypeptide Data & Comments
Reported size (kD)	2747, 2711 (aa); 300 (kD predicted) (Fischer-Vize et al., 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0005632
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C19, ubiquitin carboxyl-terminal hydrolase 2 (IPR001394) MEROPS - An information resource for peptidases and the proteins that inhibit them • C19.007
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141072   AA391465   AA697811   AA698697   AA816411   AA817335   AE003779 AAF57198   AE003779 AAN14291   AF145677 AAD38652   AI455285   AI512532   AI513421   AI516418   AQ025072   AQ254727   AY075439   BG636462   BG639134   BI162152   BI581006   BI581044   BI582005   BI582204   BI611794   BI614620   BI623462   BI628999   BT011433   BT011436   CK01157   CO181014   CO267180   CO295737   CO318547   CO328703   CO334151   CO341360   CZ467486   CZ470631   CZ471337   CZ471338   EC066839   EC074578   EC079097   EC198470   EC221462   EC238953   EC251365   EC257816   EC259369   EL875379   EL875380   EL884222   ES695822   L04958 AAF01346   L04959 AAF01345   L04960 AAF01347   L04960 AAF01348   LD44432   RH01039
UniProtKB/Swiss-Prot	P55824
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	LD22582 RE01547 RH55725 RH57784 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References complex substitution faf[B4] 3R:27,605,677..27,605,679 comment=Amino acid residue F279 is replaced by NPNNLL due to deletion of a TTT codon and insertion of 18bp (AATCCCAACAATCTACTG). evidence=experimental (Chen and Fischer, 2000) complex substitution faf[B5] 3R:27,611,960..27,611,961 comment=Frameshift after amino acid residue 2150 due to deletion of nucleotides AG and insertion of 15bp (TAATTTTTTTTTTAA) evidence=experimental (Chen and Fischer, 2000) complex substitution faf[BX5] 3R:27,613,101..27,613,102 comment=Frameshift after amino acid residue 2452, due to the nucleotides GT being changed to A. evidence=experimental (Chen and Fischer, 2000) deletion faf[BX15] 3R:27,610,210..27,610,213 comment=A four base pair deletion leads to a frame shift. evidence=experimental (Chen and Fischer, 2000) deletion faf[BX1] 3R:27,611,551..27,611,562 comment=A 12 base pair in-frame deletion leading to loss of MLFY. evidence=experimental (Chen and Fischer, 2000) point mutation faf[B3] 3R:27,604,398..27,604,398 evidence=experimental na_change=C27604398T pr_change=Q71|faf-PA,Q71|faf-PC reported_na_change=C?T reported_pr_change=Q71@ (Chen and Fischer, 2000) point mutation faf[B6] 3R:27,606,219..27,606,219 comment=TGG to TGA evidence=experimental na_change=G27606219A pr_change=W459|faf-PA,W459|faf-PC reported_na_change=G?A reported_pr_change=W459@ (Chen and Fischer, 2000) point mutation faf[B8] 3R:27,610,741..27,610,741 evidence=experimental na_change=G27610741A pr_change=E1850K|faf-PA,E1850K|faf-PC reported_na_change=G?A reported_pr_change=E1850K (Chen and Fischer, 2000) point mutation faf[FO8] 3R:27,611,279..27,611,279 evidence=experimental na_change=C27611279T pr_change=H1986Y|faf-PA,H1986Y|faf-PC reported_na_change=C?T reported_pr_change=H1986Y (Chen and Fischer, 2000) point mutation faf[B7] 3R:27,611,538..27,611,538 comment=TGG to TGA evidence=experimental na_change=G27611538A pr_change=W2051|faf-PA,W2051|faf-PC reported_na_change=G?A reported_pr_change=W2051@ (Chen and Fischer, 2000) rescue fragment faf[+t14] 3R:27,601,107..27,615,557 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=BamHI-BamHI_rfrag (Fischer-Vize et al., 1992) rescue fragment faf[+t13] 3R:27,601,925..27,615,557 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=EcoRI-BamHI_rfrag (Fischer-Vize et al., 1992) rescue fragment faf[+t17] 3R:27,601,925..27,620,157 comment=Rescue fragment extends ~4.3kb beyond a BamHI site. Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=EcoRI-END_rfrag (Fischer-Vize et al., 1992)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 11509
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  oogenesis stage,adult stage  nurse cell (Fischer-Vize et al., 1992)  oogenesis stage,adult stage  germarium (Fischer-Vize et al., 1992)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  oogenesis stage,adult stage  nurse cell (Fischer-Vize et al., 1992)  oogenesis stage,adult stage  nurse cell (Fischer-Vize et al., 1992)  oogenesis stage,adult stage | stage >=S10  oocyte | posterior (Fischer-Vize et al., 1992)  larval stage  eye-antennal disc (Fischer-Vize et al., 1992)  oogenesis stage  egg chamber (Fischer-Vize et al., 1992)  larval stage | third instar  morphogenetic furrow | anterior to (Fischer-Vize et al., 1992)  oogenesis stage,adult stage | stage S10  oocyte | posterior (Fischer-Vize et al., 1992)  oogenesis stage,larval stage  ovary (Fischer-Vize et al., 1992)  adult stage,spermatogenesis  testis (Fischer-Vize et al., 1992)  larval stage,spermatogenesis  testis (Fischer-Vize et al., 1992)  larval stage  embryonic/larval digestive system (Fischer-Vize et al., 1992)  larval stage  embryonic/larval fat body (Fischer-Vize et al., 1992)
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI512532
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0005632 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0005632 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0005632 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for faf FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for faf Stage(s) 4-6 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable fafBX4 lethal | embryonic stage | maternal effect | recessive fafFO8 Other Phenotypes Allele wild-type faf+t13 faf+t14 faf+t17 fafhs.T:Hsap\MYC fafro.T:Hsap\MYC visible fafBX4 visible (with fafBX4) fafFO8 visible (with fafFO8) fafBX4 visible | recessive fafB3 fafB4 fafB5 fafB6 fafB7 fafB8 fafBX10 fafBX11 fafBX14 fafBX15 fafBX1 fafBX3 fafBX4 fafBX5 fafBX6 fafBX7 fafBX8 fafFBB12 fafFO8 visible, with Scer\GAL4sca-537.4 fafEP381 visible, with Scer\GAL4ap-md544 fafEP381 neurophysiology defective, with Scer\GAL4elav-C155 fafEP3520 fafEP381 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 fafEP381 neuroanatomy defective, with Scer\GAL4elav-C155 fafEP3520 fafEP381 neuroanatomy defective, with Scer\GAL4Tab2-201Y fafEP381 Sterility Allele female sterile | recessive fafB3 fafB5 fafB6 fafB7 fafB8 fafBX15 fafBX1 fafBX3 fafBX4 fafBX8 fafFBB12 fafFO8 Phenotype manifest in Allele pole cell fafB3 cone cell (with fafFO8) fafBX3 eye photoreceptor cell | supernumerary (with fafFO8) fafBX3 fafBX4 ommatidium (with fafFO8) fafBX3 fafBX4 syncytial blastoderm embryo | maternal effect (with fafFO8) fafBX3 eye photoreceptor cell fafBX3 fafBX4 fafFO8 eye photoreceptor cell (with fafFO8) fafBX4 eye (with fafFO8) fafBX4 photoreceptor cell R1 | ectopic fafBX4 photoreceptor cell R2 | ectopic fafBX4 photoreceptor cell R3 | ectopic fafBX4 photoreceptor cell R4 | ectopic fafBX4 photoreceptor cell R5 | ectopic fafBX4 photoreceptor cell R6 | ectopic fafBX4 eye photoreceptor cell & ommatidium | ectopic | somatic clone fafBX4 fafFO8 eye fafBX4 ommatidium fafBX4 fafFO8 synaptic vesicle, with Scer\GAL4elav-C155 fafEP3520 synapse | supernumerary, with Scer\GAL4elav-C155 fafEP3520 fafEP381 bouton | supernumerary, with Scer\GAL4elav-C155 fafEP3520 fafEP381 dorsocentral bristle, with Scer\GAL4sca-537.4 fafEP381 trichogen cell, with Scer\GAL4sca-537.4 fafEP381 scutellar bristle, with Scer\GAL4sca-537.4 fafEP381 adult abdominal sensillum & trichogen cell, with Scer\GAL4sca-537.4 fafEP381 synapse, with Scer\GAL4elav-C155 fafEP381 anterior fascicle, with Scer\GAL4elav-C155 fafEP381 anterior fascicle & synapse, with Scer\GAL4elav-C155 fafEP381 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 fafEP381 anterior fascicle & bouton, with Scer\GAL4elav-C155 fafEP381 neuromuscular junction, with Scer\GAL4elav-C155 fafEP381 bouton, with Scer\GAL4elav-C155 fafEP381 wing sensillum | ectopic, with Scer\GAL4ap-md544 fafEP381 mushroom body, with Scer\GAL4Tab2-201Y fafEP381 ommatidium (with fafBX4) fafFO8 eye photoreceptor cell (with fafBX4) fafFO8 eye (with fafBX4) fafFO8 eye photoreceptor cell | supernumerary (with fafBX4) fafFO8 cone cell (with fafBX3) fafFO8 eye photoreceptor cell | supernumerary (with fafBX3) fafFO8 ommatidium (with fafBX3) fafFO8 syncytial blastoderm embryo | maternal effect (with fafBX3) fafFO8 eye photoreceptor cell | supernumerary fafFO8
Classical Alleles ( 29 )
For All Classical Alleles Show
Allele of faf Class Mutagen Stocks Known lesion fafB3 amorph ethyl methanesulfonate 0 -- fafB4 loss of function ethyl methanesulfonate 0 Yes fafB5 loss of function ethyl methanesulfonate 0 Yes fafB6 loss of function ethyl methanesulfonate 0 -- fafB7 amorph ethyl methanesulfonate 0 -- fafB8 amorph ethyl methanesulfonate 0 -- fafBX10 X ray 0 -- fafBX11 loss of function X ray 0 -- fafBX13 0 Yes fafBX14 X ray 0 -- fafBX15 amorph X ray 0 Yes fafBX1 amorph X ray 0 Yes fafBX3 loss of function X ray 0 Yes fafBX4 amorph, loss of function X ray 0 Yes fafBX5 X ray 0 Yes fafBX6 X ray 0 -- fafBX7 loss of function X ray 0 -- fafBX8 amorph X ray 0 -- fafBX9 0 -- fafEP3520 P-element activity 1 -- fafEP381.faf- ethyl methanesulfonate 0 -- fafEP381 P-element activity 2 Yes fafEY02018 1 -- fafFBB12 amorph ethyl methanesulfonate 0 Yes fafFO8 amorph ethyl methanesulfonate 0 -- fafc01838 piggyBac transposase 0 -- fafc06363 piggyBac transposase 1 -- fafd00030 P-element activity 1 -- fafunspecified 0 --
Alleles Carried on Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Allele of faf Class Mutagen Stocks Known lesion faf+t13 in vitro construct | genomic fragment 0 Yes faf+t14 in vitro construct | genomic fragment 0 Yes faf+t17 in vitro construct | genomic fragment 0 Yes faf392.T:Ecol\lacZ,T:Zzzz\nls1 in vitro construct | coding region fusion 0 Yes faf392.T:Ecol\lacZ in vitro construct | coding region fusion 1 Yes