Gene Dmel\trpl

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General Information

Symbol

Dmel\trpl

Species

D. melanogaster

Name

trp-like

Annotation symbol

CG18345

Feature type

protein_coding_gene

FlyBase ID

FBgn0005614

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

46B2-46B2

Sequence location

2R:5,641,222..5,653,025 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene trp-like is referred to in FlyBase by the symbol trpl (CG18345, FBgn0005614). It has the cytological map location 46B2. Its sequence location is 2R:5641222..5653025. Its molecular function is described as: calmodulin binding; calcium channel activity; store-operated calcium channel activity; light-activated voltage-gated calcium channel activity; cation channel activity; protein binding. It is involved in the biological processes: calcium ion transport; response to abiotic stimulus; response to light stimulus; calcium-mediated signaling; body fluid secretion. 14 alleles are reported. The phenotypes of these alleles are annotated with: Malpighian tubule; photoreceptor cell; photoreceptor. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

46B2-46B2

Limits computationally determined from genome sequence between P{lacW}l(2)k09221^k09221&P{PZ}dap⁰⁴⁴⁵⁴ and P{lacW}l(2)k05420^k05420

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

46A-46A

(determined by in situ hybridisation)

(Phillips et al., 1992)

46A-46A

(determined by in situ hybridisation)

(Kelly et al., 1991)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\trpl for information on other features

Comments on Gene Model

EST data support existence of multiple transcripts

EST data suggest additional 5' exon(s)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

trpl-RA

FBtr0088471

NM_057547

3750

1124

trpl-RB

FBtr0088473

NM_165694

3865

1124

trpl-RC

FBtr0088472

NM_165695

2655

766

Additional Transcript Data & Comments

Reported size (kB)

4.0 (northern blot)

(Phillips et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

trpl-PA

FBpp0087555

127.8

1124

8.98

NP_476895

AAF58904

trpl-PB

FBpp0087557

127.8

1124

8.98

NP_724822

AAM68793

trpl-PC

FBpp0087556

87.4

766

9.87

NP_724823

AAM68794

Additional Polypeptide Data & Comments

Reported size (kD)

1124 (aa); 128 (kD predicted)

(Phillips et al., 1992)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0005614

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ankyrin (IPR002110)

Transient receptor potential protein (IPR002153)

Transient receptor potential channel (IPR004729)

Ion transport (IPR005821)

Transient receptor potential II (IPR013555)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P48994

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

trpl[302]
2R:5,649,467..5,649,467

comment=site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change
evidence=experimental
pr_change=Q302@
na_change=C5649467T

(Niemeyer et al., 1996)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13172

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage

retinula cell

(Phillips et al., 1992)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

adult stage

rhabdomere

(Niemeyer et al., 1996)

Marker for

Subcellular Localization

CV Term

microvilli

(Niemeyer et al., 1996)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0005614

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0005614

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0005614

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

wild-type

trpl^hs.PN

visual behavior defective

trpl³⁰²

neurophysiology defective

trpl³⁰²

Phenotype manifest in

Allele

Malpighian tubule

trpl³⁰²

photoreceptor cell

trpl³⁰²

photoreceptor

trpl³⁰²

photoreceptor cell & axon

trpl³⁰²

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of trpl	Class	Mutagen	Stocks	Known lesion
trpl³⁰²	amorph, loss of function	ethyl methanesulfonate	0	Yes
trpl^MB03075			1	--
trpl^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Allele of trpl	Mutagen	Stocks	Known lesion
trpl^CBS1.trp	in vitro construct \| regulatory fusion	0	Yes
trpl^CBS2.trp	in vitro construct \| regulatory fusion	0	Yes
trpl^GD12722	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
trpl^H678Q.ninaE	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	0	Yes
trpl^H678R.ninaE	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	0	Yes
trpl^MtnA.PH	in vitro construct \| regulatory fusion	0	Yes
trpl^cCa	in vitro construct \| other	0	Yes
trpl^dsRNA.cRa	in vitro construct \| RNAi	0	Yes
trpl^hs.PN	in vitro construct \| regulatory fusion	0	Yes
trpl^ninaE.PW	in vitro construct \| regulatory fusion	0	Yes
trpl^trp.PS	in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12722}

characterization construct

heat-shock construct

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap binary system

Mi{ET1}trpl^MB03075

Related Comments

Please look at the allele reports for the complete phenotype data

A trpl cDNA has been cloned and sequenced.

(Phillips et al., 1992)

trpl expression in Sf9 cells is associated with appearance of Ca²⁺ permeable, non-selective cation channels.

(Hu et al., 1994)

The calmodulin-binding and ankyrin-repeats have been investigated in vitro using fusion proteins.

(Warr et al., 1994)

trpl expressed in Sf9 cells can be activated by hormonal factors after coinjection with recombinant viruses coding for various heptahelical, G-protein-coupled receptors.

(Harteneck et al., 1995)

Analysis of trp-trpl fusion proteins suggests that the carboxy terminal domain of trpl plays an important role in determining constitutive activity of the protein, and the carboxy terminal domain of trp contains structural features necessary for activation by thapsigargin.

(Sinkins et al., 1996)

The C-terminal domain of the trpl protein contains two calmodulin-binding sites.

(Warr and Kelley, 1996)

trpl activity is masked by functional trp channels and together trp and trpl are essential components of the light-activated conductance. trp and trpl need not form heteromeric channels because trpl mutants only contain trp protein and trp mutants only have trpl protein. Since trp and trpl mutants respond to light activation then each channel on its own must be capable of sensing the intracellular messenger that gates the light activated conductance. Activation of one does not require prior activation of the other.

(Niemeyer et al., 1996)

Activity of recombinant trpl in Sf9 cells is studied.

(Hu and Schilling, 1995)

Gene Ontology: Function, Process & Cellular Component ( 16 )

Molecular Function

CV term

Evidence

References

calcium channel activity

inferred from sequence or structural similarity

(Littleton and Ganetzky, 2000)

inferred from sequence or structural similarity with HGNC_gene:TRPC1; HGNC:12333; OMIM:602343 AND inferred from sequence or structural similarity with MGD:Trpc1; MGI:MGI:109528

(Zitt et al., 2002)

inferred from direct assay

(Goel et al., 2001)

calmodulin binding

inferred from sequence or structural similarity with FLYBASE:trp; FB:FBgn0003861

(Phillips et al., 1992)

traceable author statement

(Zitt et al., 2002)

traceable author statement

(Montell, 1999)

non-traceable author statement

(Swiss-Prot Project Members, 1996.2.1)