Gene Dmel\os

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General Information

Symbol

Dmel\os

Species

D. melanogaster

Name

outstretched

Annotation symbol

CG5993

Feature type

protein_coding_gene

FlyBase ID

FBgn0004956

Created / Updated

2003-12-01/2007-03-20

Genomic Location

Chromosome (arm)

Recombination map

1-58.7

Cytogenetic map

17A5-17A5

Sequence location

X:18,199,377..18,203,021 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene outstretched is referred to in FlyBase by the symbol os (CG5993, FBgn0004956). It has the cytological map location 17A5. Its sequence location is X:18199377..18203021. Its molecular function is described as: receptor binding; cytokine activity; morphogen activity. It is involved in the biological processes described with 16 unique terms, many of which group under: cell division; anatomical structure development; reproductive developmental process; gamete generation; organ morphogenesis; stem cell division; ovarian follicle cell development; sexual reproduction; sex determination; embryonic development. 36 alleles are reported. The phenotypes of these alleles are annotated with 49 unique terms, many of which group under: organ system; anatomical structure; embryonic segment; germarium; thoracic segment; follicle cell; embryonic abdomen; imaginal precursor; portion of tissue; adult; peripheral nervous system; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

os: outstretched small eye

Wings held virtually at right angles to body. Eyes small and rounded. os/oso has wing effect but eyes normal. os/oss has eye effect but wings normal. RK1.

osbdw: outstretched small eye-bending wings

Wings divergent and drooping, size and shape normal. Males sterile. RK2A.

oso: outstretched small eye-outstretched

oso: outstretched small eye-outstretched
Edith M. Wallace, unpublished.

Wings extremely divergent, often at right angles to body. oso/oss is wild type. RK1.

oss: outstretched small eye-small eye

Eyes small and rounded, high on the head but not bulging. RK1.

upd: unpaired

Embryonic lethal. Mutants show variable larval cuticular phenotype with defects predominantly in the mesothorax and the fifth abdominal segment (Nusslein-Volhard et al., 1984; Gergen and Wieschaus, 1986); also some head defects and fourth, sixth, seventh, and eighth abdominal segment defects. Heterozygotes show 88-93% viability (Wieschaus and Noell, 1986).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

17A5-17A5

Limits computationally determined from genome sequence between P{EP}ari-1^EP317 and P{EP}EP1378

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

17A5-17A7

(Sefton, 2000.2.4)

17A-17A

(Harrison, 1998.6.17)

17A-17A

(determined by in situ hybridisation)

(Cline, 1993)

17A5-17A8

(determined by in situ hybridisation)

(Eberl et al., 1992)

Experimentally Determined Recombination Data

Location

1-58.7

(Cline, 1993)

1-59.2

(Bridges and Brehme, 1944, University of Buenos Aires, 1951)

Left of (cM)

Right of (cM)

Notes

Mapping based on os^upd-1.

Mapping based on os^o.

(Bridges and Brehme, 1944)

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: anon-17Ad? os? anon-17Ae? CrebB-17A?

(Harrison et al., 1998)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\os for information on other features

Comments on Gene Model

DGC clone AT13666 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

os-RA

FBtr0074581

NM_080356

2218

413

os-RB

FBtr0112847

NM_001103545

2310

413

Additional Transcript Data & Comments

Reported size (kB)

2.2 (northern blot)

(Harrison et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

os-PA

FBpp0074353

47.0

413

12.00

NP_525095

AAF48823

os-PB

FBpp0111760

47.0

413

12.00

NP_001097015

ABW09444

Additional Polypeptide Data & Comments

Reported size (kD)

413 (aa); 47 (kD predicted)

(Harrison et al., 1998)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

AT13666

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

complex substitution

os[sisC-2]
X:18,201,269..18,201,271

comment=GTT is replaced with CGG 5bp downstream of the splice donor site, decreasing splice efficiency.
evidence=experimental

(Sefton et al., 2000)

insertion site

os[sisC-4]
X:18,201,173..18,201,173

comment=A Tyr residue is inserted after Ala 128.
evidence=experimental

(Harrison et al., 1998)

insertion site

os[upd-3]
X:18,201,127..18,201,127

comment=The dinucleotide AG inserted at this location causes a frameshift mutation.
evidence=experimental

(Harrison et al., 1998, Sefton et al., 2000)

point mutation

os[upd-4]
X:18,201,455..18,201,455

reported_na_change=C?T
reported_pr_change=Q60@
evidence=experimental
na_change=C18141359T
pr_change=Q60@|os-PA

(Harrison et al., 1998, Sefton et al., 2000)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3240

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 4,5

(Harrison et al., 1998)

embryonic stage | stage >8

embryonic/larval tracheal pit

(Harrison et al., 1998)

embryonic stage | stage 6,7

(Harrison et al., 1998)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

extracellular matrix

(Harrison et al., 1998)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004956

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0004956

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004956

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable | reduced

os^sisC-5

lethal | recessive

lethal | embryonic stage

lethal | embryonic stage | recessive

lethal | pupal stage

os^GMR.PB

semi-lethal | embryonic stage, with Df(3L)ED4238, Scer\GAL4^GMR.PF

os^Scer\UAS.cCa

Other Phenotypes

Allele

visible

visible (with Df(1)os1A2)

os^unspecified

visible | recessive

visible | somatic clone

os^d4-2

visible, with Scer\GAL4^dpp.blk1

os^Scer\UAS.cZa

visible, with Scer\GAL4^elav.PLu

os^Scer\UAS.cZa

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^ey.PH

os^Scer\UAS.cZa

increased cell number

os^GMR.PB

hyperplastic, with Scer\GAL4^C587

os^Scer\UAS.cZa

tissue polarity defective | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PI

os^Scer\UAS.cZa

tissue polarity defective | recessive | somatic clone

os^upd-4

tissue polarity defective | somatic clone, with Scer\GAL4^30A

os^Scer\UAS.cZa

Sterility

Allele

female sterile

os^sisC-5

male sterile

Phenotype manifest in

Allele

eye

eye-antennal disc

os^GMR.PB

eye disc

os^GMR.PB

ommatidium

os^GMR.PB

eye, with Scer\GAL4^GMR.PF

ommatidium, with Scer\GAL4^GMR.PF

os^Scer\UAS.cCa

eye disc, with Scer\GAL4^GMR.PF

os^Scer\UAS.cCa

ommatidium | supernumerary, with Scer\GAL4^GMR.PF

os^Scer\UAS.cCa

eye & adult, with Scer\GAL4^GMR.PF

os^Scer\UAS.cCa

border follicle cell | ectopic | somatic clone, with Scer\GAL4^Act5C.PI

cuticle & adult external head, with Scer\GAL4^ey.PH

os^Scer\UAS.cCa

eye, with Scer\GAL4^ey.PH

morphogenetic furrow, with Scer\GAL4^ey.PH

os^Scer\UAS.cCa

interfollicle cell, with Scer\GAL4^neur-P72

os^Scer\UAS.cCa

testis, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

germline stem cell | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cCa

border follicle cell | ectopic, with Scer\GAL4^slbo.2.6

os^Scer\UAS.cZa

border follicle cell, with Scer\GAL4^slbo.2.6

os^Scer\UAS.cZa

border follicle cell | supernumerary, with Scer\GAL4^slbo.2.6

os^Scer\UAS.cZa

border follicle cell | ectopic, with Scer\GAL4^c306

os^Scer\UAS.cZa

border follicle cell, with Scer\GAL4^c306

os^Scer\UAS.cZa

male germline stem cell | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

primary gonial cell | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

cyst progenitor cell | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

spermatogonium | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

round stem cell fusome | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

cyst cell | supernumerary, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

germline stem cell, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

stem cell fusome, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

cyst progenitor cell, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

os^Scer\UAS.cZa

eye, with Scer\GAL4^elav.PLu

os^Scer\UAS.cZa

eye, with Scer\GAL4^dpp.blk1

os^Scer\UAS.cZa

ommatidium | somatic clone, with Scer\GAL4^Act5C.PI

os^Scer\UAS.cZa

ommatidium, with Scer\GAL4^30A

os^Scer\UAS.cZa

eye | dorsal, with Scer\GAL4^30A

os^Scer\UAS.cZa

eye | ventral, with Scer\GAL4^30A

os^Scer\UAS.cZa

male germline stem cell | supernumerary, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

primary gonial cell | supernumerary, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

cyst progenitor cell | supernumerary, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

spermatogonium | supernumerary, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

round stem cell fusome | supernumerary, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

border follicle cell | cell non-autonomous | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

centripetally migrating follicle cell | ectopic | somatic clone, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

oocyte associated follicle cell | somatic clone, with Scer\GAL4^Act5C.PP

os^Scer\UAS.cZa

embryonic head, with Scer\GAL4^h-1J3

os^Scer\UAS.cZa

extended germ band embryo, with Scer\GAL4^da.G32

os^Scer\UAS.cZa

amnioserosa, with Scer\GAL4^da.G32

os^Scer\UAS.cZa

embryonic hindgut, with Scer\GAL4^byn-Gal4

os^Scer\UAS.cZa

embryonic hindgut, with Df(1)os1A2, Scer\GAL4^byn-Gal4

os^Scer\UAS.cZa

pole cell | ectopic, with Scer\GAL4^twi.PG

os^Scer\UAS.cZa

pole cell | ectopic, with Scer\GAL4^elav-C155

os^Scer\UAS.cZa

pole cell | ectopic, with Scer\GAL4^prd.RG1

os^Scer\UAS.cZa

germarium, with Scer\GAL4^C587

os^Scer\UAS.cZa

germline cyst | supernumerary, with Scer\GAL4^C587

os^Scer\UAS.cZa

female germline stem cell | supernumerary, with Scer\GAL4^C587

os^Scer\UAS.cZa

ovariole, with Scer\GAL4^C587

os^Scer\UAS.cZa

escort stem cell | supernumerary, with Scer\GAL4^C587

os^Scer\UAS.cZa

denticle belt & abdominal segment 4

os^UE69

denticle belt & abdominal segment 5

os^UE69

embryonic mesothoracic segment

os^UE69

embryonic metathoracic segment

embryonic abdominal segment 8

larval head & embryo

os^UE69

adult abdominal segment

os^UE69

embryonic segment

os^UE69

eye | somatic clone

polar follicle cell

os^hs.PM

interfollicle cell | supernumerary

os^hs.PM

egg chamber

os^hs.PM

oocyte

os^hs.PM

wing

border follicle cell (with os^upd-4)

os^sisC-5

tergite

os^sisC-5

border follicle cell

eye (with Df(1)os1A2)

os^unspecified

embryonic/larval proventriculus

os^upd-3

embryonic abdominal segment 4

embryonic abdominal segment 5

border follicle cell (with os^sisC-5)

os^upd-4

border follicle cell | somatic clone

os^upd-4

gonad | ectopic & embryo

os^upd-4

ommatidium | somatic clone

os^upd-4

wing, with Scer\GAL4^Bx-MS1096

os^{Scer\UAS.T:Avic\GFP-EGFP}

Classical Alleles ( 19 )

For All Classical Alleles Show

Allele of os	Class	Mutagen	Stocks	Known lesion
os¹		X ray	3	--
os^50a		spontaneous	0	--
os⁵⁴			0	--
os^UE69		γ ray	0	--
os^bdw		X ray	0	--
os^d4-2			0	Yes
os^o		X ray	7	--
os^s:sisC-1		recombination	0	--
os^s		spontaneous	16	--
os^sisC-1	loss of function		0	Yes
os^sisC-2			0	--
os^sisC-3			0	Yes
os^sisC-4			0	Yes
os^sisC-5			0	Yes
os^unspecified			0	--
os^upd-1		ethyl methanesulfonate	2	--
os^upd-2		ethyl methanesulfonate	0	--
os^upd-3		ethyl methanesulfonate	0	Yes
os^upd-4	loss of function	ethyl methanesulfonate	2	Yes

Alleles Carried on Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Allele of os	Mutagen	Stocks	Known lesion
os^Act5C.PB	in vitro construct \| regulatory fusion	0	Yes
os^{Act5C.T:Avic\GFP-EGFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
os^{Act5C.T:SV5\V5}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
os^GD1158	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
os^GMR.PB	in vitro construct \| regulatory fusion	0	Yes
os^{MtnA.T:SV5\V5}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
os^{Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
os^Scer\UAS.cCa	in vitro construct \| regulatory fusion	0	Yes
os^Scer\UAS.cZa	in vitro construct \| regulatory fusion	0	Yes
os^T:Ivir\HA1	in vitro construct \| coding region fusion	0	Yes
os^TM.Scer\UAS	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
os^fl.cHa	in vitro construct \| other	0	Yes
os^hs.PM	in vitro construct \| regulatory fusion	0	Yes
os^t10	in vitro construct \| genomic fragment	0	Yes
os^t4.8	in vitro construct \| genomic fragment	0	Yes
os^t5.8	in vitro construct \| genomic fragment	0	Yes
os^Δss	in vitro construct \| other	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)os^UE69

Disrupted in

Df(1)BSC352

(Christensen and Cook, 2007.10.29)

Df(1)N19

(Eberl et al., 1992, Harbecke and Lengyel, 1995)

Df(1)os1A2

(Agaisse et al., 2003, Eberl et al., 1992, Hombria et al., 2005)

Df(1)os^UE69

(Harrison et al., 1998, Eberl et al., 1992, Hombria et al., 2005)

In(1)sisC⁵

(Sefton et al., 1997)

Duplicated in

Dp(1;1)IMNB-8

Dp(1;1)MNB-8

Dp(1;1)NB-8

Dp(1;1)NBB-8

Dp(1;3)JC153

(Aguilera et al., 2000)

Dp(1;3)MNB-8

Not disrupted in

Df(1)274

(Patterson, 1932)

Df(1)N19

(Craymer and Roy, 1980)

In(1)Cl^Lbb^DfR

In(1)Cl^Ly^4R

In(1)y^4Lsc^4R

Tp(1;2)sn⁺72d

(Wieschaus et al., 1984)

Not duplicated in

Dp(1;3)126

Dp(1;f)100

(Morgan, 1938)

In(1)bb^DfLCl^R

Partially disrupted in

Df(1)os¹⁰⁹

(Harrison et al., 1998)

Df(1)os^4-51-1

(Harrison et al., 1998)

Df(1)os^c18

(Harrison et al., 1998)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

heat-shock construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

?{}os^sisC-1

insertion of mobile activating element

P{Mae-UAS.6.11}B1513^B1513

Related Comments

Please look at the allele reports for the complete phenotype data

os mutants display defects predominantly in mesothorax and fifth row, head defects and eighth segment defects.

(Wieschaus et al., 1984)

Mutations in zygotic cardinal gene os interact with RpII140^wimp.

(Parkhurst and Ish-Horowicz, 1991)

The os gene has been studied as part of a genetic and developmental study of polytene section 17 of the X chromosome. A duplication of this region, fcl⁺Y, has been constructed to allow mutagenesis screens and complementation analyses of the region.

(Eberl et al., 1992)

γ ray induced loss of function mutations suppress male-lethal duplications of numerator elements.

(Cline, 1993)

Overexpression of os in the testis results in expansion of both the germ line stem cell and somatic cyst progenitor cell populations.

(Tulina and Matunis, 2001)

dome

(Hombria et al., 2005)