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Gene Dmel\eIF-2β

General Information
Symbol	Dmel\eIF-2β	Species	D. melanogaster
Name	Eukaryotic initiation factor 2β	Annotation symbol	CG4153
Feature type	protein_coding_gene	FlyBase ID	FBgn0004926
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	69C4-69C4	Sequence location	3L:12,518,191..12,519,837 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Eukaryotic initiation factor 2β is referred to in FlyBase by the symbol eIF-2β (CG4153, FBgn0004926). It has the cytological map location 69C4. Its sequence location is 3L:12518191..12519837. Its molecular function is described as: translation initiation factor activity; GTP binding; tRNA binding. It is involved in the biological processes: translational initiation; formation of translation initiation ternary complex; mitotic spindle elongation; mitotic spindle organization and biogenesis; axon midline choice point recognition. 11 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 69C4-69C4   Limits computationally determined from genome sequence between P{PZ}l(3)0508805088 and P{PZ}l(3)0692406924
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\eIF-2β for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eIF-2beta-RA FBtr0075968  NM_079319   1136   312
Additional Transcript Data & Comments
Reported size (kB)	1.2 (northern blot) (Ye and Cavener, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein eIF-2beta-PA   FBpp0075700   35.2   312   5.42     NP_524043   AAF49902
Additional Polypeptide Data & Comments
Reported size (kD)	312 (aa) (Ye and Cavener, 1994)
Comments	The amino acid sequence of Eif2β shows a C2-C2 nucleic acid binding motif in the C-terminal region. (Ye and Cavener, 1994)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004926
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Translation initiation factor IF2/IF5 (IPR002735) Translation initiation factor IF2/IF5, N-terminal (IPR016189) Translation initiation factor IF2/IF5, zinc-binding (IPR016190)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA567047   AE003541 AAF49902   AI062963   AI063893   AI108692   AI134075   AI134681   AI260013   AI295150   AI296062   AI297470   AI402406   AI405480   AI455978   AI456533   AI531586   AI543148   AI543848   AI946908   AQ254621   AY071253 AAL48875   BE975302   BE976627   BI170917   BI231959   BI232045   BI233005   BI241632   BI364662   BI370234   BI569724   BI606680   BI622996   BI637019   BI637062   BI637114   BI637133   BP546884   BP547667   BP549284   BP549285   CL525980   CO191805   CO303124   CO303635   CO307067   CO322619   CO330716   CZ470350   EC065592   EC077931   EC088087   EC211919   EC225988   EC238017   EC250835   EC266200   EL877812   EL877933   L19197 AAA28504
UniProtKB/Swiss-Prot	P41375
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	bs32g12 SD02683 (Oliver, 2001.8.16) RE29270 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 4288
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Ye and Cavener, 1994)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004926 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004926
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal eIF-2βCC06208 lethal | embryonic stage eIF-2β1 eIF-2β2 lethal | embryonic stage (with Df(3L)ED4486) eIF-2β1 eIF-2β2 Other Phenotypes Allele neuroanatomy defective | embryonic stage (with Df(3L)ED4486) eIF-2β2 neuroanatomy defective | embryonic stage (with eIF-2β1) eIF-2β2 neuroanatomy defective | embryonic stage (with eIF-2β2) eIF-2β1 Phenotype manifest in Allele axon & pCC neuron (with eIF-2β2) eIF-2β1 axon & pCC neuron (with eIF-2β1) eIF-2β2 axon & pCC neuron (with Df(3L)ED4486) eIF-2β2
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of eIF-2β Class Mutagen Stocks Known lesion eIF-2β1 P-element activity 0 Yes eIF-2β2 P-element activity 0 Yes eIF-2βCC06208 P-element activity 0 Yes eIF-2βEP745 P-element activity 1 -- eIF-2βEY08063 P-element activity 1 -- eIF-2βGE06414 P-element activity 0 Yes eIF-2βGE06433 P-element activity 0 Yes eIF-2βc05909 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of eIF-2β Class Mutagen Stocks Known lesion eIF-2βGD1431 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes eIF-2βGD16736 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes eIF-2βScer\UAS.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)iro-2
Useful duplication	Dp(3;3)M67C+5
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}eIF-2βc05909 NA P{PTT-GC}eIF-2βCC06208 NA insertion of mobile activating element P{EPgy2}eIF-2βEY08063 NA P{EP}eIF-2βEP745 NA P{EP}eIF-2βGE06414 NA P{EP}eIF-2βGE06433 NA
Related Comments
	Please look at the allele reports for the complete phenotype data eIF-2β mRNA displays quasi-constitutive expression.   eIF-2β nucleotide sequence displays a high degree of similarity with the human and yeast homologs. eIF-2β mRNA displays quasi-constitutive expression. (Ye et al., 1992) eIF-2β has been isolated and characterised. (Ye and Cavener, 1994) dsRNA made from templates generated with primers directed against eIF-2β that is transfected into S2 treated with Listeria monocytogenes reveals eIF-2β to be involved in Listeria monocytogenes vacuolar escape. (Cheng et al., 2005) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
GTP binding non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
tRNA binding non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
translation initiation factor activity inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 1995.6.1) inferred from electronic annotation with InterPro:IPR002735, InterPro:IPR016189, InterPro:IPR016190 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
axon midline choice point recognition inferred from mutant phenotype (Lee et al., 2007)
formation of translation initiation ternary complex non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007)
mitotic spindle organization and biogenesis inferred from mutant phenotype (Goshima et al., 2007)
translational initiation inferred from sequence or structural similarity (Lasko, 2000) inferred from electronic annotation with InterPro:IPR002735, InterPro:IPR016189, InterPro:IPR016190 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytosol inferred from sequence or structural similarity (Lasko, 2000)
eukaryotic translation initiation factor 2 complex inferred from sequence or structural similarity (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 1995.6.1)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions eIF-2β allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 64782
Orthologs
Genome-wide drosophilid orthologs	Dana\GF24007 Dere\GG13816 Dgri\GH16843 Dmoj\GI13062 Dper\GL24944 Dpse\GA23566 Dsec\GM24645 Dsim\GD12709 Dvir\GJ13811 Dwil\GK12617 Dyak\GE20110
Curated drosophilid orthologs	Dpse\GA17991   Dsim\eIF-2β (Swanson et al., 2001)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000016768 Anopheles gambiae (Ensembl) ENSBTAP00000007832 Bos taurus (Ensembl) ENSCAFP00000011141 Canis familiaris (Ensembl) ENSCINP00000019398 Ciona intestinalis (Ensembl) NEWSINFRUP00000158030 Takifugu rubripes (Ensembl) ENSGALP00000003175 Gallus gallus (Ensembl) ENSP00000246201 Homo sapiens (Ensembl) ENSMMUP00000023780 Macaca mulatta (Ensembl) ENSMODP00000003414 Monodelphis domestica (Ensembl) ENSPTRP00000022998 Pan troglodytes (Ensembl) ENSRNOP00000023786 Rattus norvegicus (Ensembl) GSTENP00022575001 Tetraodon nigroviridis (Ensembl) ENSXETP00000042239 Xenopus tropicalis (Ensembl) O24473 Oryza sativa (Gramene) AT5G20920.1 Arabidopsis thaliana (Arabidopsis) WBGene00030053 Caenorhabditis briggsae (Wormbase) WBGene00010560 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-3187 Danio rerio (Zfin) ZDB-GENE-030131-3085 Danio rerio (Zfin) DDB0205105 Dictyostelium discoideum (Dictybase) MGI:1914454 Mus musculus (Informatics) YPL237W Saccharomyces cerevisiae (Yeastgenome) SPAC32A11.04c Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Szeged	- P{EP}eIF-2βEP745
Harvard	- PBac{PB}eIF-2βc05909
VDRC	v9416 w1118; P{GD1431}v9416/TM3 v48911 w1118; P{GD16736}v48911/CyO
Bloomington	17425 y1 w67c23; P{EPgy2}eIF-2βEY08063/TM3, Sb1 Ser1
Genomic Clones ( 1 )
	BACR48G06
cDNA Clones ( 42 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE29270
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE49829 SD18903 RE38748 RH01862 LP09865 LD36580 SD19034 GH02354 GH12211 SD02683 GH21624 RH73295 LD35750 SD18963 GH07913 GH11429 GH25491 LP11772 SD19055 LD45507 GM01015 RE12542 LP03668 RE25143 SD09767 RH55052 LP08701 RE28046 RE56683 GH04004
Other clones	IP07773 EK253436 EC32126 bs41b07 EK242258 IP07873 EK296016 EK240058 bs54a01 bs32g12 EK188517
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004926 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004926
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079319 RefSeq (Proteins) • NP_524043 DDBJ / EMBL / GenBank • AE003541 AAF49902 AI531586 AI946908 AQ254621 AY071253 AAL48875 BI233005 CL525980 CZ470350 L19197 AAA28504 UniProtKB/Swiss-Prot • P41375
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Translation initiation factor IF2/IF5 (IPR002735) Translation initiation factor IF2/IF5, N-terminal (IPR016189) Translation initiation factor IF2/IF5, zinc-binding (IPR016190)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 64782 DEDB - Drosophila exon database: splicing graphs • 4288 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004926 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004926 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004926 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004926 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004926 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000016768 Anopheles gambiae (Ensembl) ENSBTAP00000007832 Bos taurus (Ensembl) ENSCAFP00000011141 Canis familiaris (Ensembl) ENSCINP00000019398 Ciona intestinalis (Ensembl) NEWSINFRUP00000158030 Takifugu rubripes (Ensembl) ENSGALP00000003175 Gallus gallus (Ensembl)