Gene Dmel\enc

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General Information

Symbol

Dmel\enc

Species

D. melanogaster

Name

encore

Annotation symbol

CG10847

Feature type

protein_coding_gene

FlyBase ID

FBgn0004875

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

63F1-63F3

Sequence location

3L:3,818,687..3,841,208 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene encore is referred to in FlyBase by the symbol enc (CG10847, FBgn0004875). It has the cytological map location 63F1-63F3. Its sequence location is 3L:3818687..3841208. Its molecular function is described as nucleic acid binding. It is involved in the biological processes: cystoblast division; germarium-derived egg chamber formation; germarium-derived oocyte fate determination; oocyte dorsal/ventral axis determination; regulation of pole plasm oskar mRNA localization; germ-line cyst formation; mitosis; karyosome formation; oocyte axis determination; oogenesis. 31 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: organ system; anatomical structure; spiracle; embryonic/larval tracheal system; gamete; cell part; egg; karyosome; germ cell; chorion; spiracular plate; cuticle; follicle cell; chorion. It has 4 annotated transcripts and 4 annotated polypeptides.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63F1-63F3

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63F-64A

(determined by in situ hybridisation)

(Grossniklaus et al., 1989)

Experimentally Determined Recombination Data

Location

3-5.3

(Hawkins et al., 1996)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: Scsα+ Acp63F- Scsα+ enc+

(Van Buskirk et al., 2000)

Overall orientation not stated: enc+ Rdh? enc+

(Landis et al., 2003)

Gene Model & Products

Please see the GBrowse view of Dmel\enc for information on other features

Comments on Gene Model

Transposon inserted in intron (7th intron)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

enc-RA

FBtr0073153

NM_206270

7249

1823

enc-RB

FBtr0073152

NM_080026

7234

1818

enc-RD

FBtr0112844

NM_001104026

7299

1818

enc-RE

FBtr0112845

NM_001104027

7591

1937

Additional Transcript Data & Comments

Reported size (kB)

8.0, 7.0 (northern blot)

(Van Buskirk et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

enc-PA

FBpp0089267

189.3

1823

8.78

NP_995992

AAS64963

enc-PB

FBpp0073011

188.7

1818

8.78

NP_524765

AAF47799

enc-PD

FBpp0111757

188.7

1818

8.78

NP_001097496

ABW08455

enc-PE

FBpp0111758

200.5

1937

8.51

NP_001097497

ABW08456

Additional Polypeptide Data & Comments

Reported size (kD)

1548 (aa); 230 (kD observed); 170 (kD predicted)

(Van Buskirk et al., 2000)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004875

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Single-stranded nucleic acid binding R3H (IPR001374)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8MSX1

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

2242

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

adult stage, spermatogenesis

testis | restricted

(Van Buskirk et al., 2000)

adult stage, oogenesis stage | germarium region 1

female germline stem cell

(Van Buskirk et al., 2000)

adult stage, oogenesis stage | mid

oocyte | posterior

(Van Buskirk et al., 2000)

adult stage, oogenesis stage | germarium region 1

cystocyte

(Van Buskirk et al., 2000)

adult stage, oogenesis stage | stage S9

oocyte | anterior

(Van Buskirk et al., 2000)

Marker for

Subcellular Localization

CV Term

cytoplasm

(Van Buskirk et al., 2000)

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI403036
AI238560

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004875

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0004875

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004875

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

enc^BB129

lethal | maternal effect | recessive | conditional - cold sensitive

enc^R17

Other Phenotypes

Allele

mitotic cell cycle defective

mitotic cell cycle defective | recessive | conditional - heat sensitive

Sterility

Allele

female sterile

enc^A309.1M3

female sterile | recessive

enc^L32

female sterile | recessive | conditional - cold sensitive

Phenotype manifest in

Allele

karyosome

dorsal appendage

follicle cell

enc^A309.1M3

egg chamber

chorion

ovary

enc^BB129

nurse cell

oocyte nucleus

dorsal appendage | maternal effect

enc^R17

embryonic/larval posterior spiracle | maternal effect

enc^R17

filzkorper | maternal effect

enc^R17

anal pad | maternal effect

enc^R17

embryonic/first instar larval cuticle | maternal effect

enc^R17

Classical Alleles ( 29 )

For All Classical Alleles Show

Allele of enc	Class	Mutagen	Stocks	Known lesion
enc^A309.1M3		P-element activity	0	Yes
enc^BB129	gain of function	P-element activity PM hybrid dysgenesis	0	Yes
enc^BBr53		Δ2-3	0	--
enc^BBr75		Δ2-3	0	--
enc^D6		ethyl methanesulfonate	0	--
enc^DD7	gain of function	ethyl methanesulfonate	0	--
enc^KG05608		P-element activity	1	--
enc^KK7	gain of function	ethyl methanesulfonate	0	--
enc^L32		ethyl methanesulfonate	0	--
enc^M7		ethyl methanesulfonate	0	--
enc^N8		ethyl methanesulfonate	0	--
enc^OO6		ethyl methanesulfonate	0	--
enc^PdL3E36		P-element activity	0	Yes
enc^Q4		ethyl methanesulfonate	0	--
enc^R17		P-element activity	0	Yes
enc^R1	gain of function	ethyl methanesulfonate	2	--
enc^T2		ethyl methanesulfonate	0	--
enc^UU3	gain of function	ethyl methanesulfonate	0	--
enc^WW1		ethyl methanesulfonate	0	--
enc^XX1		ethyl methanesulfonate	0	--
enc^Z3		ethyl methanesulfonate	0	--
enc^c02381		piggyBac transposase	1	--
enc^c03997		piggyBac transposase	1	--
enc^c05354		piggyBac transposase	0	--
enc^c05401		piggyBac transposase	0	--
enc^d03608		P-element activity	1	--
enc^f01733		piggyBac transposase	1	--
enc^f01825		piggyBac transposase	1	--
enc^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of enc	Class	Mutagen	Stocks	Known lesion
enc^GD5997		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
enc^Hsp83.PVB		in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)A466

Df(3L)GN34

Useful duplication

Dp(3;3)SMG38

Disrupted in

Df(3L)A466

(Hawkins et al., 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD5997}

heat-shock construct

P{Hsp83-enc.VB}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{SUPor-P}enc^KG05608

insertion of enhancer trap

P{lArB}enc^A309.1M3

Yes

P{lacW}enc^BB129

insertion of mobile activating element

P{PdL}Rdh^PdL3E36

P{XP}enc^d03608

Related Comments

Please look at the allele reports for the complete phenotype data

Female sterile locus identified by P-element enhancer trap insertion.

(Grossniklaus et al., 1989)

Egg chambers from enc females produce egg chambers that contain on average 31 nurse cells and one oocyte. enc is also required later in oogenesis for correct dorsal ventral patterning in the egg. enc is a negative regulator of bam in the additional cells and the mitotic defect occurs at the beginning of the normal division program.

(Hawkins et al., 1995)

enc mutants show defects in karyosome morphology.

(Van Buskirk et al., 2000)

Gene Ontology: Function, Process & Cellular Component ( 12 )

Molecular Function

CV term

Evidence

References

nucleic acid binding

inferred from electronic annotation with InterPro:IPR001374

(FlyBase Curators et al., 2004-)

Biological Process