Gene Dmel\hts

General Information
Symbol	Dmel\hts	Species	D. melanogaster
Name	hu li tai shao	Annotation symbol	CG9325
Feature type	protein_coding_gene	FlyBase ID	FBgn0004873
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	56D3-56D5	Sequence location	2R:15,284,840..15,312,454 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene hu li tai shao is referred to in FlyBase by the symbol hts (CG9325, FBgn0004873). It has the cytological map location 56D3-56D5. Its sequence location is 2R:15284840..15312454. Its molecular function is described as: actin binding; metal ion binding. It is involved in the biological processes described with 11 unique terms, many of which group under: organelle organization and biogenesis; gamete generation; fusome organization and biogenesis; anatomical structure development; sexual reproduction; germ-line cyst formation; cell proliferation; microtubule cytoskeleton organization and biogenesis; cell division; cell cycle; ovarian nurse cell to oocyte transport; germ cell development. 54 alleles are reported. The phenotypes of these alleles are annotated with 13 unique terms, many of which group under: organ system; anatomical structure; cytoplasmic part; organism; germ cell; germarium; developing anatomical structure; intracellular organelle; cell part; fusome; intracellular membrane-bounded organelle; gamete; intracellular non-membrane-bounded organelle. It has 6 annotated transcripts and 6 annotated polypeptides.
External Summaries

Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 56D3-56D5 Limits computationally determined from genome sequence between P{PZ}ena⁰²⁰²⁹&P{lacW}cora^k08713 and P{lacW}hts^k06121
Experimentally Determined Cytological Location
Cytogenetic map Notes References 56D5-56D6 (determined by in situ hybridisation) (Spradling et al., 1999) 56D3-56D6 56D5--6 56F5--6 (BDGP Project Members, 1994-1999) 56D-56D (determined by in situ hybridisation) (Ding et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)	mei-W68 (McKim, 1998.3.10)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: l(2)k09810? hts? par-1? (McKim, 1998.3.10) In direction of increasing cytology: hts- tRNA:S4:56D+ hts- CalpA+ Fak56D- anon-56Da+ RpL11+ (Palmer et al., 1999) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\hts for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) hts-RA FBtr0086548 NM_057529 4210 1156 hts-RB FBtr0086546 NM_166347 3904 716 hts-RC FBtr0086547 NM_166351 2577 495 hts-RD FBtr0086545 NM_166350 2628 668 hts-RE FBtr0086549 NM_166348 3828 716 hts-RF FBtr0086550 NM_166349 4074 716
Additional Transcript Data & Comments
Reported size (kB)	4.4 (northern blot) (Ding et al., 1993) 4.5, 3.8, 3.7 (northern blot) (Yue and Spradling, 1992)
Comments	The structural differences between the three hts RNAs are not apparent from the paper though it is implied that all encode the same protein. The existence of 10 exons is deduced from the composite cDNA sequence which covers 3.9 kb of the 4.5 kb transcript. (Yue and Spradling, 1992, Yue and Spradling, 1992, Yue and Spradling, 1992)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein hts-PA FBpp0085732 127.9 1156 6.36 NP_476877 AAF57565 hts-PB FBpp0085730 79.1 716 6.84 NP_725886 AAF57566 hts-PC FBpp0085731 54.6 495 5.77 NP_725890 AAM70851 hts-PD FBpp0085729 73.7 668 6.07 NP_725889 AAM70850 hts-PE FBpp0085733 79.1 716 6.84 NP_725887 AAN16130 hts-PF FBpp0085734 79.1 716 6.84 NP_725888 AAN16131
Additional Polypeptide Data & Comments
Reported size (kD)	95, 87 (kD observed) (Zaccai and Lipshitz, 1996) 1156 (aa); 128 (kD) (Yue and Spradling, 1992) 1156 (aa) (Ding et al., 1993)
Comments	Monoclonal antibody 1B1 recognizes the 87kD form of hts protein. (Zaccai and Lipshitz, 1996) Antibodies were prepared against the terminal 351 amino acids of hts protein. (Robinson et al., 1994) Monoclonal antibody 2C1 recognizes the 95kD form of hts protein. (Zaccai and Lipshitz, 1996) Mammalian adducin is a membrane -cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. (Ding et al., 1993)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004873
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Class II aldolase/adducin, N-terminal (IPR001303)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391814 AA817217 AC004299 AE003796 AAF57565 AE003796 AAF57566 AE003796 AAM70850 AE003796 AAM70851 AE003796 AAN16130 AE003796 AAN16131 AF151705 AAG01377 AF151706 AAG01378 AF151707 AAG01379 AF151708 AAB59182 AF171750 AF171766 AI294697 AI517530 AI531490 AQ025783 AQ026080 AW944250 AY122245 AAM52757 BF495705 BF495706 BH840715 BI162650 BI162686 BI163157 BI163693 BI170941 BI214363 BI228070 BI229517 BI229669 BI231197 BI232032 BI236113 BI238347 BI239352 BI240778 BI243267 BI353344 BI356684 BI358477 BI368470 BI370504 BI370837 BI373266 BI566833 BI569513 BI569676 BI569804 BI581587 BI581589 BI582016 BI606140 BI612185 BI630379 BI636265 BP544421 BP544422 BT012349 CO153625 CO156022 CO178361 CO178410 CO183214 CO187388 CO187501 CO193286 CO264450 CO268533 CO269902 CO272945 CO275181 CO275871 CO277612 CO279886 CO280705 CO281565 CO284116 CO289582 CO292543 CO293413 CO294844 CO295109 CO295869 CO298463 CO298476 CO300002 CO301257 CO303649 CO305307 CO305355 CO305711 CO307007 CO317347 CO317903 CO317904 CO319507 CO320047 CO323270 CO330528 CO331673 CO332548 CO333230 CO339967 CO340746 CO340922 CO344860 CZ466323 CZ468785 CZ483758 CZ485313 CZ486720 EC057587 EC059920 EC060296 EC060427 EC064100 EC066934 EC067911 EC069103 EC070915 EC071097 EC071537 EC081155 EC082295 EC088695 EC088903 EC199392 EC200718 EC203092 EC207251 EC210429 EC210787 EC211107 EC218515 EC221151 EC222219 EC224279 EC224685 EC227083 EC230826 EC236336 EC236653 EC237592 EC237678 EC238629 EC239121 EC239761 EC240938 EC241576 EC244018 EC244247 EC245040 EC247046 EC249235 EC250016 EC251009 EC253538 EC255120 EC255882 EC257003 EC257584 EC257707 EC260886 EC263000 EL885360 EL885361 EL885398 EL885399 G01435 L05016 AAA28643

UniProtKB/Swiss-Prot	Q02645
UniProtKB/TrEMBL	A4UZP6 A1ZBK8

Maps to	DS00571 (McKim and Hayashi-Hagihara, 1998) DS07982 (McKim and Hayashi-Hagihara, 1998, FlyBase, 1992-, Palmer et al., 1999)
Does NOT map to
Identified with	AT01058