HomeGene Dmel\hop
| General Information | ||||
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| Symbol | Dmel\hop | Species | D. melanogaster | |
| Name | hopscotch | Annotation symbol | CG1594 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0004864 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | X | Recombination map | 1-34.6 | |
| Cytogenetic map | 10B5-10B6 | Sequence location | X:11,254,932..11,262,130 [-] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene hopscotch is referred to in FlyBase by the symbol hop (CG1594, FBgn0004864). It has the cytological map location 10B5-10B6. Its sequence location is X:11254932..11262130. Its molecular function is described as: Janus kinase activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; metallopeptidase activity; protein binding; zinc ion binding. It is involved in the biological processes described with 40 unique terms, many of which group under: anatomical structure development; immune response; sensory organ development; organ morphogenesis; cell division; gamete generation; ovarian follicle cell development; defense response; biopolymer modification; reproductive developmental process; embryonic pattern specification. 69 alleles are reported. The phenotypes of these alleles are annotated with 62 unique terms, many of which group under: organ system; peripheral nervous system; embryonic/larval hemocoel; nervous system; abdominal ventral denticle belt; adult segment; embryonic/larval tracheal system; follicle cell; thoracic segment; adult mesothoracic segment; portion of tissue. It has one annotated transcript and one annotated polypeptide. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | hop: hopscotch
The wild-type allele of hop is required for the continued cell division of all diploid cells as well as the
establishment of the normal array of segments. Most of the
mutants are homozygous late zygotic (L-P) lethals; one mutant
is a larval lethal; two other mutants have some adult survivors (hemizygous males being morphologaically normal, but
40% of the homzygous females and 85% of the hemizygous females
showing major defects). Most of the heteroallelic females are
lethal, with the following exceptions:
genotype percent viable
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hop29/hop25 12%
hop3/hop25 16%
hop14/hop25 40%
hop12/hop25 68%
hop27/hop25 100%
hop32/hop25 100%
hop33/hop25 100%
All viable heteroallelic combinations are female sterile,
failing to produce eggs or laying abnormal eggs that are
small, with a clear chorion and with chorionic filaments
absent or partially fused (Perrimon and Mahowald, 1986a).
There is a maternal effect on thoracic and abdominal segments,
the most extreme embryos [produced from homozygous l(1)hop
germline clones that have not received a paternal copy of
hop+] showing defects in the posterior spiracles and in segments T2 (denticle belt deleted). T3, A4, and A5 (segment
missing) and A8 (segment reduced in size); the least extreme
mutant embryos from germline clones show defects in segment
A5. Defects visible in early segmentation stages. The extent
of the defects is dependent on the strength of the maternal
alleles and the paternal contribution. Wild-type sperm can
rescue all defects, except those in A5. A few of the rescued
progeny hatch and develop into adults.
Tum: Tumorous
Dominant tumorous gene that is a temperature-sensitive lethal at 29 in hemizygous males and homozygous
females; about two-thirds of the hemizygous males survive at
18 and one-quarter of these have melanotic tumors. Males
raised at 26 and heterozygous females raised at 29 survive to
adulthood, but show melanotic masses in the abdominal cavity
or small black specks in the legs, wings, or thorax. Mutant
larvae kept at 29 show enlargement of the lymph glands in the
late second- or early third-instar larvae, but no melanotic
masses. By mid third-instar, the lymph glands are large and
diffuse and the gastric caeca have become encapsulated and
melanized. By late third-instar, the larvae have melanotic
masses in the body cavity, lack lymph glands, and have
reduced, encapsulated and melanized gastric caeca as well as
encapsulated and melanized muscles and fat bodies. These
mutants do not survive beyond the late third-instar or the
early pupal stage. When lymph glands from Tum larvae are
injected into adult female hosts, transplantable neoplasms are
produced. Melanization, at first associated with the leg
joints and later with the head, thorax, and abdomen, takes
place; also abdominal bloating. The lymph glands become
melanotic and the abdomen is filled with encapsulated masses
before the premature death of the injected individuals.
Injection of Tum tissue other than lymph glands fails to produce these effects. The melanotic neoplasms can be transplanted into a succession of hosts in which they produce the
same abnormalities. The neoplastic cells resemble hemocytes;
some cell lines are melanotic and others are unpigmented, but
in both types, the tissue, when transplanted, grows rapidly in
the hosts and kills them.
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 10B5-10B6
Limits computationally determined from genome sequence between P{EP}CG11756EP1610 and P{EP}CG32666EP1452
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 10B6-10B6 (determined by in situ hybridisation)
10B6-10B6 (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | 1-34.6 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: dsh+ anon-10Ba- hop- Gene Order (overall orientation not stated) References | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\hop
for information on other features
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| Comments on Gene Model | ||||
DGC clone HL03036 appears problematic: incomplete CDS | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 5.4, 5.1 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 1177 (aa) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Band 4.1, N-terminal (IPR000299)
Protein kinase, core (IPR000719)
SH2 motif (IPR000980)
Tyrosine protein kinase (IPR001245)
Tyrosine protein kinase, active site (IPR008266)
Protein kinase-like (IPR011009)
Protein kinase ATP binding, conserved site (IPR017441)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References deletion hop[4] X:11,260,891..11,260,895 comment=Approximate endpoints of a 5bp deletion reported to be at residue 201 within the JH6 domain. evidence=experimental deletion hop[3] X:11,256,018..11,256,021 comment=Approximate endpoints of a 4bp deletion reported to be at residue 1121 at the C-terminal end of the kinase domain. evidence=experimental point mutation hop[20]-1 X:11,261,467..11,261,467 comment=One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G11261467A pr_change=R10K|hop-PA reported_pr_change=R10K point mutation hop[J1A.1] X:11,261,237..11,261,237 comment=hop[J1A.1] was generated in a hop[Tum] background. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The mutation is within the JH7 domain. evidence=experimental na_change=C11261237T pr_change=L87F|hop-PA reported_pr_change=L87F point mutation hop[9] X:11,260,973..11,260,973 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G11260973M pr_change=G175R|hop-PA reported_pr_change=G175R point mutation hop[25] X:11,260,760..11,260,760 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C11260760A pr_change=Q246K|hop-PA reported_pr_change=Q246K point mutation hop[34] X:11,260,553..11,260,553 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=A11260553T pr_change=K315@|hop-PA reported_pr_change=K315@ point mutation hop[Tum] X:11,260,474..11,260,474 evidence=experimental na_change=G11260474A pr_change=G341E|hop-PA reported_na_change=G1641A reported_pr_change=G341E point mutation hop[T42] X:11,258,124..11,258,124 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G11258124A pr_change=E695K|hop-PA reported_pr_change=E695K point mutation hop[13] X:11,257,870..11,257,871 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The A592G substitution, also observed in the mutant strain, appears to be a polymorphism, since a G residue is present at this site in the reference sequence. evidence=experimental pr_change=W741@|hop-PA reported_pr_change=W741@ point mutation hop[29] X:11,257,775..11,257,775 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C11257775A pr_change=S773@|hop-PA reported_pr_change=S773@ point mutation hop[20]-2 X:11,257,349..11,257,349 comment=One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C11257349R pr_change=Y892@|hop-PA reported_pr_change=Y892@ point mutation hop[F1.1] X:11,257,207..11,257,207 point mutation hop[27] X:11,256,488..11,256,488 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=T11256488C pr_change=I1011T|hop-PA reported_pr_change=I1011T point mutation hop[14] X:11,256,263..11,256,263 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C11256263T pr_change=S1062F|hop-PA reported_pr_change=S1062F point mutation hop[12] X:11,255,991..11,255,991 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C11255991T pr_change=Q1131@|hop-PA reported_pr_change=Q1131@ point mutation hop[32] X:11,255,980..11,255,981 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=W1134@|hop-PA reported_pr_change=W1134@ | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
embryonic stage | early
embryonic stage-adult stage | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele semi-lethal (with Df(3L)ED4238) Other Phenotypes Allele tumorigenic (with Df(3L)ED4238) Sterility Allele Phenotype manifest in Allele | ||||