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Gene Dmel\otk

General Information
Symbol	Dmel\otk	Species	D. melanogaster
Name	off-track	Annotation symbol	CG8967
Feature type	protein_coding_gene	FlyBase ID	FBgn0004839
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48D6-48D7	Sequence location	2R:7,888,983..7,907,329 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene off-track is referred to in FlyBase by the symbol otk (CG8967, FBgn0004839). It has the cytological map location 48D6-48D7. Its sequence location is 2R:7888983..7907329. Its molecular function is described as: cell adhesion molecule binding; protein kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; protein-tyrosine kinase activity; semaphorin receptor binding; ATP binding. It is involved in the biological processes: cell adhesion; protein amino acid phosphorylation; axon guidance. 11 alleles are reported. The phenotypes of these alleles are annotated with: axon; abdominal posterior fascicle; anterior fascicle; medulla. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48D6-48D7   Limits computationally determined from genome sequence between P{lacW}l(2)k06612k06612 and P{lacW}jebk05644
Experimentally Determined Cytological Location
Cytogenetic map Notes References 48D-48D (determined by in situ hybridisation)   (Pulido et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\otk for information on other features
Comments on Gene Model
	Has significant BLAST similarity to the upstream gene CG8964.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) otk-RA FBtr0088028  NM_078981   4644   1033
Additional Transcript Data & Comments
Reported size (kB)	5.7 (northern blot) (Pulido et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein otk-PA   FBpp0087135   114.3   1033   6.88     NP_523705   AAF58596
Additional Polypeptide Data & Comments
Reported size (kD)	1033 (aa); 160 (kD observed) (Pulido et al., 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004839
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) Tyrosine protein kinase (IPR001245) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Immunoglobulin-like (IPR007110) Tyrosine protein kinase, active site (IPR008266) Protein kinase-like (IPR011009) Immunoglobulin I-set (IPR013098) Immunoglobulin (IPR013151) Immunoglobulin-like fold (IPR013783)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003823 AAF58596   AI404212   AQ074029   BT015249 AAT94478   CK661771   CO184108   CO310470   CO344963   CQ847776   CZ482896   EC053991   EC054489   EC054972   EC062718   EC064675   EC072593   EC203414   EC213736   EC255182   EC255985   X63453 CAA45053
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24327 Q6AWJ9
Maps to
Does NOT map to
Identified with	LP17455 (BDGP Project Members, 2000-) D1264 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12867
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage,pupal stage   (Pulido et al., 1992)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage 13  embryonic/larval hindgut (Pulido et al., 1992)  embryonic stage | stage 11  posterior midgut primordium (Pulido et al., 1992)  embryonic stage | stage 13  embryonic/larval midgut (Pulido et al., 1992)  embryonic stage | stage 11  epidermis | restricted (Pulido et al., 1992)  embryonic stage | late  posterior commissure (Pulido et al., 1992)  embryonic stage | stage 13  visceral mesoderm (Pulido et al., 1992)  embryonic stage | stage 12  maxillary segment (Pulido et al., 1992)  embryonic stage | late  peripheral nervous system | restricted (Pulido et al., 1992)  embryonic stage | stage 13  ventral nerve cord (Pulido et al., 1992)  embryonic stage | stage 12  epidermis | segmentally repeated (Pulido et al., 1992)  embryonic stage | late  axon (Pulido et al., 1992)  embryonic stage | stage 12  proctodeum | primordium (Pulido et al., 1992)  embryonic stage | late  cell body (Pulido et al., 1992)  embryonic stage | stage 7  cephalic furrow (Pulido et al., 1992)  embryonic stage | stage 7-11  anterior midgut primordium (Pulido et al., 1992)  embryonic stage | stage 11  neuroblast (Pulido et al., 1992)  embryonic stage | stage 12-17  neuron (Pulido et al., 1992)  embryonic stage | stage 11  procephalic segment (Pulido et al., 1992)  embryonic stage | late  anterior commissure (Pulido et al., 1992)
Marker for
Subcellular Localization
CV Term	plasma membrane (Pulido et al., 1992)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004839 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004839 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004839
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable otk2 otk8 semi-lethal, with Scer\GAL4elav.PLu otkEP2017 Other Phenotypes Allele wild-type otk2 otk8 Phenotype manifest in Allele axon otk1 otk3 abdominal posterior fascicle otk1 otk3 anterior fascicle otk1 otk3 photoreceptor cell R2 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R3 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R4 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R5 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 photoreceptor cell R6 & axon & larva (with otkEP2017), with Scer\GAL4[-]: otk3 eye photoreceptor cell & axon & larva | somatic clone otk3 photoreceptor cell R1 & axon & larva | somatic clone otk3 photoreceptor cell R2 & axon & larva | somatic clone otk3 photoreceptor cell R3 & axon & larva | somatic clone otk3 photoreceptor cell R4 & axon & larva | somatic clone otk3 photoreceptor cell R5 & axon & larva | somatic clone otk3 photoreceptor cell R6 & axon & larva | somatic clone otk3 lamina & glial cell & larva | somatic clone otk3 photoreceptor cell R1 & axon | somatic clone otk3 photoreceptor cell R2 & axon | somatic clone otk3 photoreceptor cell R3 & axon | somatic clone otk3 photoreceptor cell R4 & axon | somatic clone otk3 photoreceptor cell R5 & axon | somatic clone otk3 photoreceptor cell R6 & axon | somatic clone otk3 medulla | somatic clone otk3 photoreceptor cell R2 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R3 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R4 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R5 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 photoreceptor cell R6 & axon & larva (with otk3), with Scer\GAL4[-]: otkEP2017 axon, with Scer\GAL4elav.PLu otkEP2017 abdominal posterior fascicle, with Scer\GAL4elav.PLu otkEP2017 anterior fascicle, with Scer\GAL4elav.PLu otkEP2017
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of otk Class Mutagen Stocks Known lesion otk1 0 -- otk2 0 Yes otk3 loss of function 0 Yes otk8 0 Yes otkEP2017 P-element activity 2 Yes otkf00431 piggyBac transposase 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of otk Class Mutagen Stocks Known lesion otkGD14399 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes otkGD2562 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes otkGD66 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes otkScer\UAS.cWa in vitro construct | regulatory fusion 0 Yes otkcPa in vitro construct | other 0 Yes
Aneuploid Aberrations
Useful duplication	Dp(2;2)Y3b
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14399} NA P{GD2562} NA P{GD66} NA P{UAS-otk.W} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}otkEP2017 NA miscellaneous insertions PBac{WH}otkf00431 NA
Related Comments
	Please look at the allele reports for the complete phenotype data otk has been identified, sequence and its expression pattern characterised. (Pulido et al., 1992) The otk gene product promotes cell adhesion in a homophilic calcium- dependent manner and this adhesion process specifically activates its kinase behaviour. (Pulido, 1993)
Gene Ontology: Function, Process & Cellular Component ( 11 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266 (FlyBase Curators et al., 2004-)
cell adhesion molecule binding inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992)
protein kinase activity inferred from direct assay (Pulido et al., 1992)
protein tyrosine kinase activity non-traceable author statement (Morrison et al., 2000) inferred from sequence or structural similarity with NCBI_gi:4506273 (FlyBase, 1992-)
receptor activity inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992)
semaphorin receptor binding traceable author statement (Castellani and Rougon, 2002)
transmembrane receptor protein tyrosine kinase activity inferred from sequence or structural similarity (Pulido et al., 1992)
Biological Process
CV term Evidence References
axon guidance inferred from genetic interaction with FLYBASE:Sema-1a; FB:FBgn0011259 AND inferred from genetic interaction with FLYBASE:plexA; FB:FBgn0025741 AND inferred from mutant phenotype (Winberg et al., 2001) inferred from mutant phenotype (Cafferty et al., 2004)
cell adhesion inferred from direct assay AND inferred from sequence or structural similarity (Pulido et al., 1992)
protein amino acid phosphorylation non-traceable author statement (Morrison et al., 2000) inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR008266 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Pulido et al., 1992)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions otk allele Gene References otk3 Sema-1a (Winberg et al., 2001) unspecified plexA (Winberg et al., 2001)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62067
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12649 Dere\GG22613 Dgri\GH21048 Dmoj\GI18649 Dper\GL11545 Dpse\GA21443 Dsec\GM20393 Dsim\GD25868 Dvir\GJ21665 Dwil\GK21983 Dyak\GE13482
Curated drosophilid orthologs	Dpse\GA21443
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000014155 Anopheles gambiae (Ensembl) ENSBTAP00000016963 Bos taurus (Ensembl) ENSCAFP00000002651 Canis familiaris (Ensembl) ENSCINP00000001678 Ciona intestinalis (Ensembl) NEWSINFRUP00000182959 Takifugu rubripes (Ensembl) ENSGALP00000014004 Gallus gallus (Ensembl) ENSP00000230419 Homo sapiens (Ensembl) ENSMMUP00000015320 Macaca mulatta (Ensembl) ENSMODP00000023690 Monodelphis domestica (Ensembl) ENSPTRP00000031080 Pan troglodytes (Ensembl) GSTENP00025110001 Tetraodon nigroviridis (Ensembl) ENSXETP00000023797 Xenopus tropicalis (Ensembl) MGI:1918711 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Szeged	- P{EP}otkEP2017
Harvard	- PBac{WH}otkf00431
VDRC	v878 w1118; P{GD66}v878 v879 w1118; P{GD66}v879 v42565 w1118; P{GD2562}v42565 v42566 w1118; P{GD2562}v42566 v30833 w1118; P{GD14399}v30833 v30834 w1118; P{GD14399}v30834
Bloomington	17231 w1118; P{EP}otkEP2017/CyO
Genomic Clones ( 2 )
	BACR14L19 BACR04E21
cDNA Clones ( 9 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP17455
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH23842 LP21473
Other clones	EC054489 EK221760 EC053991 EC054972 EC27129 EP21032
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004839 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004839
Antibody Information
	polyclonal (Pulido et al., 1992)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	Identification: By sequence similarity to human trk (receptor tyrosine kinase) cDNA probes. (Pulido et al., 1992) Phylogenetic analysis of the PTK family. (Suga et al., 1997) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078981 RefSeq (Proteins) • NP_523705 DDBJ / EMBL / GenBank • AE003823 AAF58596 AQ074029 BT015249 AAT94478 CQ847776 CZ482896 X63453 CAA45053 UniProtKB/TrEMBL • Q24327 Q6AWJ9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, core (IPR000719) Tyrosine protein kinase (IPR001245) Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Immunoglobulin-like (IPR007110) Tyrosine protein kinase, active site (IPR008266) Protein kinase-like (IPR011009) Immunoglobulin I-set (IPR013098) Immunoglobulin (IPR013151) Immunoglobulin-like fold (IPR013783)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62067 DEDB - Drosophila exon database: splicing graphs • 12867 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004839 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004839 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004839 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004839 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004839 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004839 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000014155 Anopheles gambiae (Ensembl) ENSBTAP00000016963 Bos taurus (Ensembl) ENSCAFP00000002651 Canis familiaris (Ensembl) ENSCINP00000001678 Ciona intestinalis (Ensembl) NEWSINFRUP00000182959 Takifugu rubripes (Ensembl) ENSGALP00000014004 Gallus gallus (Ensembl) ENSP00000230419 Homo sapiens (Ensembl) ENSMMUP00000015320 Macaca mulatta (Ensembl) ENSMODP00000023690 Monodelphis domestica (Ensembl) ENSPTRP00000031080 Pan troglodytes (Ensembl) GSTENP00025110001 Tetraodon nigroviridis (Ensembl) ENSXETP00000023797 Xenopus tropicalis (Ensembl) MGI:1918711 Mus musculus (Informatics) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/offtrack1.htm PANTHER - Protein classification by function, families, and pathways • FBgn0004839
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	anon-WO2004063362.81   CG8967 (Morrison et al., 2000, ) CT25769 (Hynes and Zhao, 2000) Dtkr   Dtrk (Hidalgo, 2002, Jaaro et al., 2001, Goodman, 1999.8.19, Suga et al., 1997, Pulido, 1993, Pulido et al., 1991,