Gene Dmel\Hrb27C

General Information
Symbol	Dmel\Hrb27C	Species	D. melanogaster
Name	Heterogeneous nuclear ribonucleoprotein at 27C	Annotation symbol	CG10377
Feature type	protein_coding_gene	FlyBase ID	FBgn0004838
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	27C4-27C4	Sequence location	2L:6,920,710..6,926,122 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Heterogeneous nuclear ribonucleoprotein at 27C is referred to in FlyBase by the symbol Hrb27C (CG10377, FBgn0004838). It has the cytological map location 27C4. Its sequence location is 2L:6920710..6926122. Its molecular function is described as: mRNA 3'-UTR binding; mRNA 5'-UTR binding; single-stranded DNA binding; mRNA binding; nucleotide binding. It is involved in the biological processes: negative regulation of oskar mRNA translation; regulation of pole plasm oskar mRNA localization; mRNA processing; pole plasm oskar mRNA localization; regulation of nuclear mRNA splicing, via spliceosome; border follicle cell migration; positive regulation of translation. 21 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: egg; organ system; adult segment; adult mesothoracic segment; anatomical structure; peripheral nervous system; chorion; nervous system; acellular anatomical structure; cell part. It has 3 annotated transcripts and 3 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27C4-27C4 Limits computationally determined from genome sequence between P{lacW}xl6^k00230&P{EP}Hrb27C^EP748 and P{PZ}smt3⁰⁴⁸⁴¹&P{PZ}smt3⁰⁴⁴⁹³
Experimentally Determined Cytological Location
Cytogenetic map Notes References 27C4-27C5 (determined by in situ hybridisation) (Abdelilah-Seyfried et al., 2000) 27C4-27C5 (determined by in situ hybridisation) (Spradling et al., 1999) 27C4-27C5 (BDGP Project Members, 1994-1999) 27C-27C (determined by in situ hybridisation) (Matunis et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: CG13773- CG10354+ BEST:LD39226- CG10354+ wee- nop5- xl6- Hrb27C+ (Price et al., 2000) Gene Order (overall orientation not stated) References Overall orientation not stated: Hrb27C? wee+ (Campbell et al., 1995) Overall orientation not stated: Hrb27C- xl6+ nop5+ xl6+ wee+ (Vorbruggen et al., 2000, Vorbruggen et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\Hrb27C for information on other features
Comments on Gene Model
	EST data suggest additional 3' exon(s)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hrb27C-RA FBtr0079346 NM_057521 2303 421 Hrb27C-RB FBtr0079348 NM_164720 2284 421 Hrb27C-RC FBtr0079347 NM_164721 2306 421
Additional Transcript Data & Comments
Reported size (kB)	1.7 (longest cDNA) (Matunis et al., 1992) 4.1, 2.1 (northern blot) (Siebel et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Hrb27C-PA FBpp0078974 44.8 421 6.79 NP_476869 AAF52456 Hrb27C-PB FBpp0078976 44.8 421 6.79 NP_723228 AAF52457 Hrb27C-PC FBpp0078975 44.8 421 6.79 NP_723229 AAN10605
Additional Polypeptide Data & Comments
Reported size (kD)	48 (kD observed) (Matunis et al., 1992) 385 (aa); 48 (kD observed); 41 (kD predicted) (Matunis et al., 1992) 422 (aa) (Siebel et al., 1994)
Comments	hnRNP-specific mAbs were obtained and used to purify hnRNP complexes from S2 cells. hnRNP proteins were identified on the basis of nucleoplasmic localization, association with nascent transcripts, crosslinking to poly(A)-containing RNA in living cells, and amino acid sequence. Staining of polytene chromosomes reveals that Hrb27C protein is associated with actively transcribing loci. (Matunis et al., 1992) Hrb protein was purified by RNA affinity chromatography. It was shown to recognize specific nucleotides in a pseudo-5\\' splice site within the inhibitory element of the P-element third intron. A correction to the published sequence is presented which predicts a larger size for the Hrb27C protein. (Siebel et al., 1994) Hrb27C protein has two amino-terminal RNP-CS RNA-binding domains and a carboxy-terminal glycine-rich auxiliary domain. (Matunis et al., 1992)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004838
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif, RNP-1 (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201942 AA202609 AA202913 AA202944 AA203069 AA246433 AA246593 AA263228 AA263575 AA390552 AA390858 AA390950 AA391089 AA391163 AA391295 AA391332 AA391422 AA391496 AA391560 AA391999 AA392686 AA392762 AA438304 AA439533 AA439832 AA441275 AA536463 AA538637 AA538967 AA539049 AA539264 AA539349 AA540098 AA540289 AA540299 AA540307 AA540381 AA540389 AA540610 AA541008 AA567200 AA567270 AA567924 AA567930 AA695337 AA696949 AA697325 AA735449 AA735867 AA735957 AA802364 AA802407 AA802494 AA802735 AA802821 AA816550 AA816595 AA817139 AA817315 AA941218 AA941516 AA942230 AA949248 AA949404 AA950448 AA951235 AA952154 AA978501 AA979536 AC004277 AC007977 AE003615 AAF52456 AE003615 AAF52457 AE003615 AAN10605 AI113664 AI135622 AI239156 AI260866 AI293384 AI295092 AI403813 AI405439 AI454977 AI454994 AI455044 AI512950 AI513747 AI515149 AI516132 AI516617 AI518743 AI531613 AI533261 AI533500 AI542362 AI546131 AQ026496 AQ073272 AW941032 AW943872 AY069699 AAL39844 AY128430 AAM75023 BG634427 BG636374 BG640998 BI166336 BI166370 BI166776 BI170031 BI170151 BI171901 BI172728 BI173696 BI212116 BI212816 BI213279 BI213451 BI214812 BI215874 BI216169 BI216850 BI228574 BI229999 BI230265 BI230470 BI230804 BI231144 BI232216 BI232556 BI232787 BI232813 BI234861 BI235603 BI235742 BI236711 BI240179 BI241722 BI242223 BI242986 BI351965 BI353123 BI353265 BI353558 BI354913 BI355827 BI356882 BI357050 BI357081 BI362993 BI363852 BI363979 BI364466 BI365026 BI365034 BI365413 BI365535 BI367194 BI367812 BI368865 BI370139 BI371012 BI372637 BI372817 BI372878 BI373007 BI373476 BI374337 BI374609 BI374800 BI374931 BI375367 BI375409 BI375451 BI481755 BI481860 BI482485 BI482948 BI483081 BI483204 BI485139 BI485296 BI485354 BI485423 BI485438 BI485821 BI486388 BI486841 BI578107 BI592734 BI592758 BI609585 BI609927 BI616817 BI632108 BI632622 BI637849 BP541931 BP544439 BP547679 BP549651 BP549652 CG10377 CL868580 CO153449 CO153813 CO155770 CO156271 CO263911 CO264883