Gene Dmel\mdy

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General Information

Symbol

Dmel\mdy

Species

D. melanogaster

Name

midway

Annotation symbol

CG31991

Feature type

protein_coding_gene

FlyBase ID

FBgn0004797

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

36B1-36B2

Sequence location

2L:16,816,665..16,826,991 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene midway is referred to in FlyBase by the symbol mdy (CG31991, FBgn0004797). It has the cytological map location 36B1-36B2. Its sequence location is 2L:16816665..16826991. Its molecular function is described as: diacylglycerol O-acyltransferase activity; sterol O-acyltransferase activity. It is involved in the biological processes: regulation of nurse cell apoptosis; triacylglycerol biosynthetic process; oogenesis. 7 alleles are reported. The phenotype of these alleles is annotated with egg chamber. It has 4 annotated transcripts and 4 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

36B1-36B2

Limits computationally determined from genome sequence between P{lacW}Mhc^k10423&P{lacW}Cas^k03902 and P{lacW}Aac11^k06710

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

36A10-36A10

(Buszczak, 2002.1.14)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\mdy for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

mdy-RA

FBtr0080915

NM_135969

2112

565

mdy-RB

FBtr0080916

NM_165194

2039

565

mdy-RC

FBtr0080917

NM_165195

2359

565

mdy-RD

FBtr0080918

NM_206002

2336

615

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mdy-PA

FBpp0080472

64.9

565

9.66

NP_609813

AAN10972

mdy-PB

FBpp0080473

64.9

565

9.66

NP_724016

AAN10973

mdy-PC

FBpp0080474

64.9

565

9.66

NP_724017

AAF53574

mdy-PD

FBpp0089387

70.7

615

10.06

NP_995724

AAS64717

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004797

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Membrane bound O-acyl transferase, MBOAT (IPR004299)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

LD33852

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

mdy[RF48]
2L:16,826,388..16,826,388

evidence=experimental
reported_pr_change=V477D
pr_change=V477D|mdy-PC,V527D|mdy-PD,V477D|mdy-PA,V477D|m dy-PB
comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
na_change=T16826388A

(Buszczak et al., 2002)

point mutation

mdy[QX25]
2L:16,826,514..16,826,515

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified)
evidence=experimental
pr_change=W549|mdy-PD,W499|mdy-PB,W499|mdy-PC, W499|m dy-PA
reported_pr_change=W499@

(Buszczak et al., 2002)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

7848

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AA979341

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004797

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0004797

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004797

Alleles & Phenotypes

Summary of Allele Phenotypes

Sterility

Allele

female sterile

female sterile (with mdy^QX25)

mdy^k03902

female sterile (with mdy^QX25), with Scer\GAL4[-]:

mdy^EP2592

female sterile (with mdy^RF48)

mdy^k03902

female sterile (with mdy^RF48), with Scer\GAL4[-]:

mdy^EP2592

female sterile (with mdy^k03902)

female sterile (with mdy^EP2592), with Scer\GAL4[-]:

female sterile | recessive

Phenotype manifest in

Allele

nurse cell & nucleus

mdy^QX25

egg chamber

mdy^QX25

nurse cell & nuclear membrane

mdy^RF48

Classical Alleles ( 6 )

For All Classical Alleles Show

Allele of mdy	Mutagen	Stocks	Known lesion
mdy⁰¹		0	Yes
mdy^EP2592	P-element activity	0	Yes
mdy^KG08905	P-element activity	1	--
mdy^QX25	ethyl methanesulfonate	2	Yes
mdy^RF48	ethyl methanesulfonate	0	Yes
mdy^k03902	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mdy	Class	Mutagen	Stocks	Known lesion
mdy^GD1749		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(2L)H20

Useful duplication

Dp(2;2)m-3

Disrupted in

Df(2L)Exel6039

(Ryder, 2004.4.26)

Df(2L)Exel8036

(Ryder, 2004.4.26)

Df(2L)H20

(Schupbach and Wieschaus, 1991)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1749}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Cas^EP2592

P{XP}Cas^d09893

miscellaneous insertions

P{SUPor-P}mdy^KG08905

P{UASp-YFP.Rab14.S49N}Cas⁰¹

insertion of enhancer trap

P{lacW}Cas^k03902

Related Comments

Please look at the allele reports for the complete phenotype data

Mutations at the mdy locus cause defects in midoogenesis.

(Schupbach and Wieschaus, 1991)

Egg chambers undergo premature nurse cell actin bundling, nuclear membrane permeabilization, cell death and degeneration in mdy mutant females.

(Buszczak et al., 2002)

Gene Ontology: Function, Process & Cellular Component ( 5 )

Molecular Function

CV term

Evidence

References

diacylglycerol O-acyltransferase activity

non-traceable author statement

(Buszczak, 2002.1.14)

inferred from direct assay

(Buszczak et al., 2002)

inferred from sequence or structural similarity with EMBL:AY065621; protein_id:AAL49962.1

(FlyBase Genome Annotators, 2002-2003)

sterol O-acyltransferase activity

inferred from sequence or structural similarity

(Buszczak et al., 2000)

Biological Process

CV term

Evidence

References

oogenesis

inferred from mutant phenotype

(Schupbach and Wieschaus, 1991)

regulation of nurse cell apoptosis

inferred from mutant phenotype

(Buszczak et al., 2002)

triacylglycerol biosynthetic process

inferred from sequence or structural similarity with EMBL:AY065621; protein_id:AAL49962.1

(FlyBase Genome Annotators, 2002-2003)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mdy allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

69359

Orthologs

Genome-wide drosophilid orthologs

Dana\GF15369

Dere\GG22776

Dgri\GH13104

Dmoj\GI14192

Dper\GL18793

Dpse\GA16599

Dsec\GM17212

Dsim\GD24087

Dvir\GJ17509

Dwil\GK18239

Dyak\GE12771

Curated drosophilid orthologs

Dpse\GA16599

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000002281
Anopheles gambiae (Ensembl)
ENSBTAP00000037256
Bos taurus (Ensembl)
ENSCINP00000007268
Ciona intestinalis (Ensembl)
NEWSINFRUP00000165462
Takifugu rubripes (Ensembl)
ENSP00000332258
Homo sapiens (Ensembl)
ENSP00000340350
Homo sapiens (Ensembl)
ENSMMUP00000001118
Macaca mulatta (Ensembl)
ENSMODP00000013384
Monodelphis domestica (Ensembl)
ENSPTRP00000035395
Pan troglodytes (Ensembl)
ENSRNOP00000035110
Rattus norvegicus (Ensembl)
GSTENP00034052001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000003061
Xenopus tropicalis (Ensembl)
Q5Z8Z2
Oryza sativa (Gramene)
AT2G19450.1
Arabidopsis thaliana (Arabidopsis)
WBGene00041524
Caenorhabditis briggsae (Wormbase)
WBGene00010408
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-1325
Danio rerio (Zfin)
ZDB-GENE-030131-4600
Danio rerio (Zfin)
DDB0202877
Dictyostelium discoideum (Dictybase)
MGI:1333825
Mus musculus (Informatics)
SPCP1E11.05c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 10 )

v6367

w¹¹¹⁸; P{GD1749}v6367

v6369

w¹¹¹⁸; P{GD1749}v6369

Kyoto

102288

Df(1)w67c23, y¹ ; P{lacW}Cas^k03902 / CyO

108039

mdy^QX25 cn¹ bw¹/CyO, l(2)DTS513¹

Bloomington

15147

y¹; P{SUPor-P}mdy^KG08905; ry⁵⁰⁶

23263

y¹ w^*; P{UASp-YFP.Rab14.S49N}Cas⁰¹/CyO

5095

mdy^QX25 cn¹ bw¹/CyO, l(2)DTS513¹

10536

y¹ w^67c23; P{lacW}Cas^k03902 mdy^k03902/CyO

Genomic Clones ( 1 )

BACR27M18

cDNA Clones ( 23 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

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FBgn0004797

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0004797

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG17938 CG17937

(Whitfield, 2000.12.15)

Source for merge of: CG17938 CG13273 Source for merge of CG17938 CG13273 was a shared cDNA (date:010720).

(FlyBase, 1996-)

Additional comments

Source for split of: CG17938 Release 2 annotation CG17938 split into CG31739 and CG31991 (mdy) in release 3 of the genome annotation. In addition, CG31991 contains sequences corresponding to release 2 annotation CG13273.

(FlyBase Genome Annotators, 2002-2003)

Comments About Role

Comments About Molecular Function

Mutant egg chambers contain severely reduced levels of neutral lipids in the germline. In direction of increasing cytology: mdy+ Cas- mdy+

(Buszczak et al., 2002)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•