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Gene Dmel\ex

General Information
Symbol	Dmel\ex	Species	D. melanogaster
Name	expanded	Annotation symbol	CG4114
Feature type	protein_coding_gene	FlyBase ID	FBgn0004583
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-0.1
Cytogenetic map	21C2-21C2	Sequence location	2L:431,253..448,701 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene expanded is referred to in FlyBase by the symbol ex (CG4114, FBgn0004583). It has the cytological map location 21C2. Its sequence location is 2L:431253..448701. Its molecular function is described as protein binding. It is involved in the biological processes described with 14 unique terms, many of which group under: regulation of biological process; anatomical structure development; cell communication; regulation of developmental process; cell proliferation; sensory organ development; signal transduction; organ morphogenesis; endocytic recycling; organ development; transforming growth factor beta receptor signaling pathway; cell cycle. 30 alleles are reported. The phenotypes of these alleles are annotated with 42 unique terms, many of which group under: adult segment; peripheral nervous system; nervous system; adult mesothoracic segment; metatarsus; imaginal precursor; metathoracic metatarsus; organ system; thoracic segment; larval thorax. It has one annotated transcript and one annotated polypeptide.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	brl: braille (M.P. Scott) Recessive mutation with complete penetrance and variable expressivity. Strongest phenotypes include partial to full transformation of one or both eyes into antennae. Less severe adult phenotypes include excess bristles under the eyes, duplication of the more anterior set of scutellar bristles (macrochaetae), and a spoon-like curvature of the wings. Homozygotes are viable and fertile. ex: expanded ex: expanded From Stern and Bridges, 1926, Genetics 11: 503-30. Wings extremely wide and large, sometimes curved and divergent. Effect produced in prepupal wing, probably by influence on cell division (Waddington, 1940, J. Genet. 41: 75-139). Eyes slightly reduced in size and roughish. Body large. RK2.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21C2-21C2   Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
Experimentally Determined Cytological Location
Cytogenetic map Notes References 21C4-21C5 (determined by in situ hybridisation)   (Spradling et al., 1999) 21C-21C (determined by in situ hybridisation)   (Kania et al., 1995) 21C3-21C4 21C4--5   (BDGP Project Members, 1994-1999) 21C1-21C4 (determined by in situ hybridisation)   (Boedigheimer and Laughon, 1993)
Experimentally Determined Recombination Data
Location	2-0.1   2-0.65 (Huettner and Bryant, 1988)
Left of (cM)
Right of (cM)
Notes	Mapped with exl2ey. (Huettner and Bryant, 1988)
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: ex? crq? ush? (Franc et al., 1999)
Gene Model & Products
Please see the GBrowse view of Dmel\ex for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ex-RA FBtr0078059  NM_057492   6646   1427
Additional Transcript Data & Comments
Reported size (kB)	6.9 (northern blot) (Boedigheimer and Laughon, 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein ex-PA   FBpp0077719   153.6   1427   8.45     NP_476840   AAF51495
Additional Polypeptide Data & Comments
Reported size (kD)	1429 (aa) (Boedigheimer and Laughon, 1993)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004583
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Band 4.1, N-terminal (IPR000299) Pleckstrin homology-type (IPR011993) FERM/acyl-CoA-binding protein, 3-helical bundle (IPR014352)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC004275   AC005269   AC005762   AE003589 AAF51495   AI109403   AQ026045   AQ073263   AY069068 AAL39213   BI615631   BI629197   BP551695   BP551696   BP556162   BP556163   CA806578   CL705831   CO153711   CO193022   CO272464   CO281959   CO282692   CO282717   CO315666   EC061497   EC064726   EC067140   EC075041   EC075375   EC085039   EC090714   EC197605   EC197896   EC222509   EC238287   EC239688   EC263677   L14768 AAB39774
UniProtKB/Swiss-Prot	Q07436
UniProtKB/TrEMBL
Maps to	DS05054 DS00764 (FlyBase, 1992-)
Does NOT map to
Identified with	GH08582 (BDGP Project Members, 2000-, Silva, 2006) Dm0205 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References deletion ex[e1] 2L:429,761..437,244 comment=Approximate mapping of ex[e1] deletion to a restriction fragment; ex[e1] consists of a partial excision of the original P{lacW}ex[697] insert plus removal of 3-12kb of flanking genomic sequence; position of restriction fragment on reference sequence inferred by FlyBase curator evidence=experimental linked_to=HindIII-HindIII_rfrag (Boedigheimer and Laughon, 1993)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 5805
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  larval stage  imaginal disc (Boedigheimer and Laughon, 1993)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  larval stage  dorsal mesothoracic disc (Boedigheimer and Laughon, 1993)  larval stage  imaginal disc (Boedigheimer and Laughon, 1993)  larval stage  ventral thoracic disc (Boedigheimer and Laughon, 1993)
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI109403
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004583 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004583 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004583
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable exEY08756 exbrl lethal | recessive ex01270 ex02278 exe1 exe2 exe6 exk01108 exk01115 exk12913 exl2ey lethal | pharate adult stage | recessive exe1 semi-lethal ex01270 Other Phenotypes Allele visible ex1 ex2 visible | recessive ex697 exbrl visible | recessive | conditional - cold sensitive exl2ey visible | somatic clone ex01270 visible, with Scer\GAL432B exC.Scer\UAS exScer\UAS.cBa visible, with Scer\GAL4Bx-MS1096 exScer\UAS.cBa visible, with Scer\GAL4dpp.blk1 exGS2228 exScer\UAS.cBa visible, with Scer\GAL4hs.2sev exScer\UAS.cBa visible, with Scer\GAL4bi-omb-Gal4 exScer\UAS.cBa visible, with Scer\GAL4GMR.PF exScer\UAS.cBa visible, with Scer\GAL4ey.PH exScer\UAS.cBa reduced cell size | recessive ex697 increased cell number ex697 uncoordinated | recessive exl2ey cell death increase, with Scer\GAL4hs.2sev exScer\UAS.cBa cell death increase, with Scer\GAL4bi-omb-Gal4 exScer\UAS.cBa cell death increase, with Scer\GAL4GMR.PF exScer\UAS.cBa cell death increase, with Scer\GAL4ey.PH exScer\UAS.cBa mitotic cell cycle defective ex697 hyperplastic ex1 hyperplastic | recessive exe1 hyperplastic | somatic clone exNY1 tissue polarity defective | somatic clone ex01270 exe1 tissue polarity defective, with Scer\GAL4hs.2sev exScer\UAS.cBa Sterility Allele fertile exEY08756 exbrl Phenotype manifest in Allele eye | somatic clone ex01270 exe1 ommatidium | somatic clone ex01270 exe1 imaginal disc ex02278 wing ex1 ex2 ex697 exbrl exe1 exl2ey eye ex1 ex2 exbrl exl2ey adult thorax ex1 ex2 adult abdomen ex1 ex2 wing & prepupa ex1 leg ex697 exe1 thorax ex697 anterior crossvein ex697 posterior crossvein ex697 eye-antennal disc & third instar larva ex697 interommatidial bristle & eye disc & pupa ex697 dorsal mesothoracic disc ex697 exe1 wing, with Scer\GAL432B exC.Scer\UAS exScer\UAS.cBa anterior crossvein | ectopic, with Scer\GAL432B exC.Scer\UAS exScer\UAS.cBa wing vein, with Scer\GAL432B exC.Scer\UAS exScer\UAS.cBa wing margin, with Scer\GAL432B exC.Scer\UAS exScer\UAS.cBa wing vein, with Scer\GAL4dpp.blk1 exGS2228 eye, with Scer\GAL432B exScer\UAS.cBa ommatidium, with Scer\GAL432B exScer\UAS.cBa pigment cell, with Scer\GAL432B exScer\UAS.cBa eye | anterior, with Scer\GAL4hs.2sev exScer\UAS.cBa ommatidium, with Scer\GAL4hs.2sev exScer\UAS.cBa pigment cell, with Scer\GAL4hs.2sev exScer\UAS.cBa eye photoreceptor cell, with Scer\GAL4hs.2sev exScer\UAS.cBa cone cell, with Scer\GAL4hs.2sev exScer\UAS.cBa eye, with Scer\GAL4hs.2sev exScer\UAS.cBa eye, with Scer\GAL4ey.PH exScer\UAS.cBa eye disc, with Scer\GAL4ey.PH exScer\UAS.cBa ommatidium, with Scer\GAL4ey.PH exScer\UAS.cBa eye, with Scer\GAL4GMR.PF exScer\UAS.cBa wing, with Scer\GAL4bi-omb-Gal4 exScer\UAS.cBa leg, with Scer\GAL4bi-omb-Gal4 exScer\UAS.cBa wing vein, with Scer\GAL4bi-omb-Gal4 exScer\UAS.cBa wing, with Scer\GAL4Bx-MS1096 exScer\UAS.cBa wing margin, with Scer\GAL4Bx-MS1096 exScer\UAS.cBa leg, with Scer\GAL4Bx-MS1096 exScer\UAS.cBa 1st posterior cell, with Scer\GAL4dpp.blk1 exScer\UAS.cBa vibrissae exbrl anterior scutellar bristle exbrl head exe1 prothoracic tarsal segment 3 exe1 prothoracic tarsal segment 4 exe1 prothoracic tarsal segment 5 exe1 mesothoracic tarsal segment 3 exe1 mesothoracic tarsal segment 4 exe1 mesothoracic tarsal segment 5 exe1 metathoracic tarsal segment 3 exe1 metathoracic tarsal segment 4 exe1 metathoracic tarsal segment 5 exe1 arista exe1 ommatidium exe1 antenna exe1 exl2ey dorsal metathoracic disc exe1 ventral prothoracic disc exe1 ventral mesothoracic disc exe1 ventral metathoracic disc exe1 eye-antennal disc exe1 exl2ey eye disc exe1 photoreceptor cell | somatic clone exe1 eye disc | somatic clone exe1 postgenal sensillum exl2ey antennal sensillum exl2ey leg | conditional - cold sensitive exl2ey adult head | conditional - cold sensitive exl2ey larval optic lobe exl2ey
Classical Alleles ( 21 )
For All Classical Alleles Show
Allele of ex Class Mutagen Stocks Known lesion ex01270 P-element activity 0 -- ex02278 P-element activity 0 -- ex1 spontaneous 8 -- ex2 natural gas 0 -- ex697 P-element activity 0 Yes exEY08756 P-element activity 1 -- exGS2228 Δ2-3 1 Yes exNY1 ethyl methanesulfonate 0 -- exbrl P-element activity 0 -- exe1 amorph, loss of function P-element activity 0 Yes exe2 P-element activity 0 -- exe3 P-element activity 0 -- exe4 P-element activity 0 -- exe5 P-element activity 0 -- exe6 loss of function P-element activity 0 -- exk01108 P-element activity 1 -- exk01115 P-element activity 1 -- exk05915a P-element activity 0 -- exk12913 P-element activity 3 -- exl2ey ethyl methanesulfonate 0 -- exunspecified 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ex Class Mutagen Stocks Known lesion ex1-1429.MtnA in vitro construct | regulatory fusion 0 Yes ex1-399.MtnA in vitro construct | regulatory fusion 0 Yes ex1066-1429.MtnA.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes ex773-1065.MtnA.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes ex773-1429.MtnA.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes exC.Scer\UAS in vitro construct | regulatory fusion 0 Yes exGD12782 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes exN.Scer\UAS in vitro construct | regulatory fusion 0 Yes exScer\UAS.cBa in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)al
Useful duplication	Dp(2;2)M24F+14
Disrupted in	Df(2L)BSC107 (Deal and Cook, 2005.3.10) Df(2L)S5 (Lewis, 1945) Df(2L)TE99(Z)XW88 (Franc et al., 1999) Df(2L)al (Franc et al., 1999, Spradling et al., 1999, Korochkina and Golubovsky, 1978, Huettner and Bryant, 1988, Lewis, 1945, BDGP Project Members, 1994-1999, Tyler et al., 2007)
Not disrupted in	Df(2L)S1 (Lewis, 1945) Df(2L)S2 (Lewis, 1945) Df(2L)S3 (Lewis, 1945) Df(2L)S4 (Lewis, 1945) Df(2L)ast1 (BDGP Project Members, 1994-1999) Df(2L)ast2 (Spradling et al., 1999) In(2L)ast-1 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12782} NA P{exC} NA P{exN} NA P{ex} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{?lacW}exe1 NA insertion of enhancer trap P{PZ}ex01270 Yes P{PZ}ex02278 Yes P{lacW}ex697 Yes P{lacW}exk01108 No P{lacW}exk01115 No P{lacW}exk05915a No P{lacW}exk12913 No P{lacW}l(2)SH0348SH0348 No P{lacZ}exbrl No insertion of mobile activating element P{EPgy2}exEY08756 NA P{EP}EP2428 NA P{EP}EP511 NA P{GSV1}C580 NA P{GSV1}exGS2228 NA
Related Comments
	Please look at the allele reports for the complete phenotype data The ex gene is necessary for proper growth control of imaginal discs. (Boedigheimer and Laughon, 1993) Mutants display hyperplastic phenotype, with imaginal disc overgrowth. (Mechler, 1994)
Gene Ontology: Function, Process & Cellular Component ( 18 )
Molecular Function
CV term Evidence