Gene Dmel\5-HT7

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General Information

Symbol

Dmel\5-HT7

Species

D. melanogaster

Name

Serotonin receptor 7

Annotation symbol

CG12073

Feature type

protein_coding_gene

FlyBase ID

FBgn0004573

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

100B1-100B1

Sequence location

3R:26,794,864..26,842,569 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Serotonin receptor 7 is referred to in FlyBase by the symbol 5-HT7 (CG12073, FBgn0004573). It has the cytological map location 100B1. Its sequence location is 3R:26794864..26842569. Its molecular function is described as: serotonin receptor activity; G-protein coupled serotonin receptor activity; amine receptor activity; dopamine receptor activity. It is involved in the biological processes: serotonin receptor, adenylate cyclase activating pathway; serotonin receptor signaling pathway. 5 alleles are reported. The phenotype of these alleles is annotated with Malpighian tubule. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

100B1-100B1

Limits computationally determined from genome sequence between P{lacW}l(3)s2500^s2500 and P{PZ}Aph-4⁰⁷⁰²⁸&P{PZ}dco^rK215

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

100A-100A

(determined by in situ hybridisation)

(Boschert et al., 1991.12.17)

100A-100A

(determined by in situ hybridisation)

(Boschert et al., 1991)

100A-100A

(determined by in situ hybridisation)

(Saudou et al., 1992)

99F-100A

(determined by in situ hybridisation)

(Witz et al., 1990)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\5-HT7 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

5-HT7-RA

FBtr0085746

NM_079860

3478

564

Additional Transcript Data & Comments

Reported size (kB)

5.5 (northern blot)

(Saudou et al., 1992, Witz et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

5-HT7-PA

FBpp0085108

60.9

564

8.06

NP_524599

AAF57104

Additional Polypeptide Data & Comments

Reported size (kD)

564 (aa)

(Witz et al., 1990)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004573

Crossreferences

GPCRDB - Information system for G protein-coupled receptors

•

0023

InterPro domains - A database of protein families, domains, and functional sites

•

GPCR, rhodopsin-like (IPR000276)

Dopamine receptor (IPR000929)

Serglycin (IPR007455)

GPCR, rhodopsin-like superfamily (IPR017452)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P20905

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

LD04507

(BDGP Project Members, 2000-)

D1183

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11440

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 16-17

ventral midline

(Saudou et al., 1992)

adult stage

adult head

(Witz et al., 1990)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004573

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004573

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for 5-HT7

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for 5-HT7

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

5-HT7^f05214

Sterility

Allele

fertile

5-HT7^f05214

Phenotype manifest in

Allele

Malpighian tubule, with Scer\GAL4^c42

5-HT7^Scer\UAS.cKa

Malpighian tubule, with Scer\GAL4^tsh-c724

5-HT7^Scer\UAS.cKa

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of 5-HT7	Mutagen	Stocks	Known lesion
5-HT7^MB01344		1	--
5-HT7^MB04445		1	--
5-HT7^f05214	piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of 5-HT7	Class	Mutagen	Stocks	Known lesion
5-HT7^GD704		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
5-HT7^Scer\UAS.cKa		in vitro construct \| regulatory fusion	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)tll-e

Useful duplication

Dp(3;3)516

Disrupted in

Df(3R)Exel6260

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD704}

P{UAS-5-HT7.K}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}5-HT7^f05214

insertion of enhancer trap binary system

Mi{ET1}5-HT7^MB01344

Mi{ET1}5-HT7^MB04445

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 9 )

Molecular Function

CV term

Evidence

References

G-protein coupled serotonin receptor activity

non-traceable author statement

(Swiss-Prot Project Members, 1991.2.1)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

amine receptor activity

inferred from sequence or structural similarity

(Radford et al., 2002)

dopamine receptor activity

inferred from electronic annotation with InterPro:IPR000929

(FlyBase Curators et al., 2004-)

serotonin receptor activity

traceable author statement

(Tierney, 2001)

inferred from sequence or structural similarity with UniProtKB:Q64264

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

serotonin receptor signaling pathway

inferred from sequence or structural similarity with UniProtKB:Q64264

(FlyBase, 1992-)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

serotonin receptor, adenylate cyclase activating pathway

traceable author statement

(Tierney, 2001)

non-traceable author statement

(Swiss-Prot Project Members, 1991.2.1)

Cellular Component

CV term

Evidence

References

integral to membrane

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from electronic annotation with InterPro:IPR000276, InterPro:IPR000929

(FlyBase Curators et al., 2004-)

integral to plasma membrane

non-traceable author statement

(Swiss-Prot Project Members, 1991.2.1)

plasma membrane

inferred from sequence or structural similarity with MGD:Htr2c; MGI:MGI:96281

(FlyBase, 1992-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

5-HT7 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68519

Orthologs

Genome-wide drosophilid orthologs

Dana\GF23300

Dere\GG11898

Dgri\GH14351

Dmoj\GI10445

Dper\GL13974

Dpse\GA11376

Dsec\GM12116

Dsim\GD16708

Dvir\GJ10661

Dwil\GK13099

Dyak\GE23347

Curated drosophilid orthologs

Dpse\GA11376

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000012916
Anopheles gambiae (Ensembl)
ENSCINP00000009162
Ciona intestinalis (Ensembl)
NEWSINFRUP00000169577
Takifugu rubripes (Ensembl)
ENSMODP00000008519
Monodelphis domestica (Ensembl)
WBGene00025201
Caenorhabditis briggsae (Wormbase)
WBGene00004780
Caenorhabditis elegans (Wormbase)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Harvard

PBac{WH}5-HT7^f05214

Bloomington

18848

w¹¹¹⁸; PBac{WH}5-HT7^f05214

23066

y¹ w^67c23; Mi{ET1}5-HT7^MB01344

24705

w¹¹¹⁸; Mi{ET1}5-HT7^MB04445 CG31008^MB04445

Genomic Clones ( 2 )

cDNA Clones ( 18 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones