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Gene Dmel\mew

General Information
Symbol	Dmel\mew	Species	D. melanogaster
Name	multiple edematous wings	Annotation symbol	CG1771
Feature type	protein_coding_gene	FlyBase ID	FBgn0004456
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	11E3-11E8	Sequence location	X:13,108,442..13,152,523 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene multiple edematous wings is referred to in FlyBase by the symbol mew (CG1771, FBgn0004456). It has the cytological map location 11E3-11E8. Its sequence location is X:13108442..13152523. Its molecular function is described as: cell adhesion molecule binding; receptor activity. It is involved in the biological processes described with 17 unique terms, many of which group under: anatomical structure development; cell adhesion; open tracheal system development; organ development; organ morphogenesis; cell motility; cell-cell adhesion; gut development; cell-substrate adhesion; heterophilic cell adhesion; imaginal disc-derived wing margin morphogenesis. 30 alleles are reported. The phenotypes of these alleles are annotated with 17 unique terms, many of which group under: organ system; nervous system; anatomical structure; primordium; peripheral nervous system; embryonic nervous system; embryonic/larval tracheal system; endoderm derivative; extended germ band embryo; posterior midgut proper primordium; embryonic dorsal branch; embryonic/larval visceral branch; midgut constriction; cephalopharyngeal skeleton; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 11E3-11E8   Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640EP1626
Experimentally Determined Cytological Location
Cytogenetic map Notes References 11E-11F (determined by in situ hybridisation) 11E1--4 (determined by in situ hybridisation) 11D1--2 (determined by in situ hybridisation)   (Peter et al., 2002) 11E-11F (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 11E1-11E4 (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12) 11D7-11E4 (determined by in situ hybridisation)   (Wehrli and Wilcox, 1990) 11D7-11E5 (determined by in situ hybridisation)   (Wehrli et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\mew for information on other features
Comments on Gene Model
	Shares 5' UTR with upstream gene.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mew-RA FBtr0073731  NM_167354   3861   1049 mew-RB FBtr0073732  NM_078590   4989   1146
Additional Transcript Data & Comments
Reported size (kB)	5.4 (northern blot) (Wehrli et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein mew-PA   FBpp0073562   117.1   1049   5.77     NP_727679   AAN09652 mew-PB   FBpp0073563   128.0   1146   6.27     NP_511145   AAF48242
Additional Polypeptide Data & Comments
Reported size (kD)	1146 (aa); 128 (kD predicted) (Wehrli et al., 1993)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004456
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Integrins alpha chain (IPR000413) Integrin alpha chain, C-terminal cytoplasmic region (IPR013513) FG-GAP (IPR013517) Integrin alpha beta-propellor (IPR013519) Integrin alpha-2 (IPR013649)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003491 AAF48242   AE003491 AAN09652   AI238462   AI259685   AI405434   AJ426890   AJ426891   AQ073209   AW940555   AY075338 AAL68203   BF500887   BI355439   BP547131   BT023656   CK659338   CL610246   CO182742   CO184866   CO192770   CO303940   CO338792   CZ468171   CZ490208   EC078824   EC083448   EC199104   EC201305   EC201968   EC202381   EC208983   EC219155   EC240553   EC265357   EL872991   EL872992   X73975 CAA52155   Z32452
UniProtKB/Swiss-Prot	Q24247
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	GH14509 GM30781 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2665
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  larval stage | third instar  adepithelial cell of dorsal mesothoracic disc (Wehrli et al., 1993)  embryonic stage  posterior embryonic/larval midgut (Wehrli et al., 1993)  larval stage | third instar  dorsal mesothoracic disc (Wehrli et al., 1993)  embryonic stage  embryonic central nervous system (Wehrli et al., 1993)  larval stage | third instar  peripodial epithelium (Wehrli et al., 1993)  embryonic stage | stage 12  tracheal pit (Bradley et al., 2003)  embryonic stage  ectoderm (Wehrli et al., 1993)  embryonic stage | stage 11-12  embryonic salivary gland (Bradley et al., 2003)  embryonic stage  anterior embryonic/larval midgut (Wehrli et al., 1993)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI238462
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004456 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004456 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004456 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for mew FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mew Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable mewA1-5 mewEY09631 mewe03932 lethal | recessive mew023 mew114 mew134 mew498 mew81 mewG0429 mewG0443 mewH10 mewM6 mewPG30 lethal | larval stage mewG0429 lethal | larval stage | recessive mewH7 mewM6 semi-lethal | male mewG0443 semi-lethal | female mewG0443 Other Phenotypes Allele visible | recessive | conditional ts mewA1-5 neuroanatomy defective mewM6 Sterility Allele fertile mewEY09631 mewe03932 Phenotype manifest in Allele extended germ band embryo mew498 mewM6 anterior midgut proper primordium mew498 mewM6 posterior midgut proper primordium mew498 mewM6 midgut constriction mew498 mewM6 embryonic peripheral nervous system mew498 mewM6 embryonic central nervous system mew498 mewM6 wing mew498 mewH10 photoreceptor mew498 larval midgut mew498 mew81 mewH7 mewM6 wing | somatic clone mew498 mewM6 stage S14 oocyte mew498 retina | somatic clone mew498 mewM6 dorsal wing | somatic clone mew81 retina mew81 wing | conditional ts mewA1-5 embryonic salivary gland mewM6 embryonic digestive system mewM6 larval midgut & embryo mewM6 fascicle mewM6 embryonic/larval visceral branch mewM6 embryonic dorsal branch, with Scer\GAL4btl.PS mewScer\UAS.cWa
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of mew Class Mutagen Stocks Known lesion mew023 amorph X ray 2 -- mew114 amorph X ray 0 -- mew134 X ray 0 -- mew498 amorph, loss of function ethyl methanesulfonate 0 -- mew81 amorph ethyl methanesulfonate 0 -- mewA1-5 0 -- mewEP1365 P-element activity 1 -- mewEY09631 P-element activity 1 -- mewG0078a P-element activity 1 -- mewG0429 P-element activity 1 -- mewG0443 P-element activity 1 -- mewH10 amorph ethyl methanesulfonate 0 Yes mewH7 ethyl methanesulfonate 0 Yes mewM6 amorph, loss of function ethyl methanesulfonate 2 Yes mewP13 0 -- mewPG30 Δ2-3 0 -- mewc02739 piggyBac transposase 1 -- mewe03932 piggyBac transposase 2 -- mewf07434 piggyBac transposase 1 -- mewunspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mew Class Mutagen Stocks Known lesion mewGD1230 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes mewMtnA.PG in vitro construct | regulatory fusion 0 Yes mewScer\UAS.cMBa in vitro construct | regulatory fusion 0 Yes mewScer\UAS.cMBb in vitro construct | regulatory fusion 0 Yes mewScer\UAS.cWa in vitro construct | regulatory fusion 0 Yes mewcBa in vitro construct | other 0 Yes mewhs.PB in vitro construct | regulatory fusion 0 Yes mewΔ.Scer\UAS in vitro construct | deletion 0 Yes mewΔ.hs in vitro construct | deletion in vitro construct | regulatory fusion 0 Yes mewΔcyt.Scer\UAS in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)N12
Disrupted in	Df(1)N12 (Walsh and Brown, 1998, Brower et al., 1995) In(1)mewM6 (Brower et al., 1995)
Duplicated in	Dp(1;f)y+ (Brower et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{UAS-if::mew.αPS1/2} NA P{UAS-if::mew.αPS2/1} NA UAS construct P{GD1230} NA P{UAS-mew.MB} NA P{UAS-mew.W} NA P{UAS-mew.c/g} NA P{UAS-mew.Δcyt} NA P{UAS-mew.Δ} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}mewEY09631 NA P{EP}mewEP1365 NA miscellaneous insertions PBac{PB}mewc02739 NA PBac{RB}mewe03932 NA PBac{WH}mewf07434 NA insertion of enhancer trap binary system P{GawB}mewPG30 No insertion of enhancer trap P{lacW}mewG0078a No P{lacW}mewG0429 No P{lacW}mewG0443 No
Related Comments
	Please look at the allele reports for the complete phenotype data Phenotypic analysis of mew, if and mys embryos suggests multiple roles for PS integrins in the adhesion of cells and in the formation, organization and migration of embryonic tissues. Although the proteins are often expressed in adjacent embryonic tissues, this distribution does not necessarily reflect equivalent requirements. The complete loss of both α subunits, encoded by mew and if, does not produce all the phenotypes observed in embryos lacking the mys encoded β subunit. (Roote and Zusman, 1995) Loss of mew produces disorganisation along the basal surface of the retina. if function can replace mew function in the retina. (Roote and Zusman, 1996)
Gene Ontology: Function, Process & Cellular Component ( 20 )
Molecular Function
CV term Evidence References
cell adhesion molecule binding inferred from mutant phenotype (Roote and Zusman, 1995) inferred from sequence or structural similarity (Hynes and Zhao, 2000)
receptor activity inferred from mutant phenotype (Roote and Zusman, 1995) inferred from sequence or structural similarity (Hynes and Zhao, 2000)
Biological Process
CV term Evidence References
apposition of dorsal and ventral imaginal disc-derived wing surfaces non-traceable author statement (Brower, 2003)
axon guidance inferred from mutant phenotype (Stevens and Jacobs, 2002)
cell adhesion inferred from mutant phenotype (Roote and Zusman, 1995)
cell differentiation traceable author statement (Brown, 2000)
cell migration traceable author statement (Brown, 2000)
cell-cell adhesion traceable author statement (Brown, 2000)
cell-matrix adhesion inferred from sequence or structural similarity (Hynes and Zhao, 2000)
ectodermal gut morphogenesis traceable author statement (Brown, 2000)
epithelial cell migration, open tracheal system traceable author statement (Uv et al., 2003) traceable author statement (Rosin and Shilo, 2002)
heterophilic cell adhesion traceable author statement (Hynes and Zhao, 2000)
imaginal disc-derived wing morphogenesis inferred from expression pattern AND inferred from mutant phenotype (Brabant et al., 1996) traceable author statement (Brown, 2000)
maintenance of epithelial integrity, open tracheal system inferred from genetic interaction with FLYBASE:if; FB:FBgn0001250 (Levi et al., 2006)
midgut development traceable author statement (Brown, 2000)
open tracheal system development non-traceable author statement (Ribeiro et al., 2003)
salivary gland boundary specification inferred from mutant phenotype (Kolesnikov and Beckendorf, 2005)
salivary gland development traceable author statement (Abrams et al., 2003)
tracheal outgrowth, open tracheal system traceable author statement (Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
integrin complex inferred from sequence or structural similarity with HGNC_gene:ITGA3; HGNC:6139; OMIM:605025 (Brown, 2000) inferred from sequence or structural similarity (Hynes and Zhao, 2000) non-traceable author statement (Brower, 2003) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mew allele Gene References mew023 if (Roote and Zusman, 1995, Li et al., 1998) mew498 sog (Araujo et al., 2003) if (Roote and Zusman, 1995, Roote and Zusman, 1996, Li et al., 1998) mewA1-5 bs (Walsh and Brown, 1998) moa (Walsh and Brown, 1998) blo (Walsh and Brown, 1998) pot (Walsh and Brown, 1998) mewM6 sog (Araujo et al., 2003) if (Roote and Zusman, 1995, Roote and Zusman, 1996) sli (Stevens and Jacobs, 2002) scb (Martin-Bermudo et al., 1999) if::mew (Martin-Bermudo et al., 1997) pot (Walsh and Brown, 1998) mewScer\UAS.cWa ap (O'Keefe and Thomas, 2001) mewunspecified if (Prokop et al., 1998) unspecified mys (Jannuzi et al., 2004) if (Levi et al., 2006) H (Mueller et al., 2005) fra (