Gene Dmel\LysP

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: LysB+ LysC+ LysD- LysE+ LysP- LysS+

Gene Model & Products

Please see the GBrowse view of Dmel\LysP for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

LysP-RA

FBtr0072633

NM_057480

616

141

Additional Transcript Data & Comments

Reported size (kB)

0.6 (northern blot)

(Kylsten et al., 1992, Daffre et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

LysP-PA

FBpp0072529

15.6

141

8.82

NP_476828

AAF47452

Additional Polypeptide Data & Comments

Reported size (kD)

141 (aa)

Comments

External Data

(BDGP Project Members, 1994-1999)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004429

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glycoside hydrolase, family 22, lysozyme (IPR000974)

Glycoside hydrolase, family 22 (IPR001916)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P29615

UniProtKB/TrEMBL

Maps to

DS07291

(FlyBase, 1992-)

Does NOT map to

Identified with

D1282

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

DEDB - Drosophila exon database: splicing graphs

•

1907

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage

adult salivary gland

adult stage

adult salivary gland

adult stage

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004429

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0004429

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004429

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of LysP	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of LysP	Class	Mutagen	Stocks	Known lesion
LysP^GD17546		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
LysP^GD5896		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(3L)Ar14-8

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{LysP-lacZ.V}

UAS construct

P{GD17546}

P{GD5896}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

LysP has been cloned, sequenced and its developmental expression pattern analysed. The transcription levels of LysP decrease after bacterial injections into the haemocoel. The LysP gene product is therefore not induced as part of the immune response, but instead appears to have a role in the digestion of bacteria present in fermenting food.

Lysozymes are expressed at a high level mainly in the digestive tract, they are not a component of the haemolymph. Lysozyme is not induced by bacteria so is not part of the inducible immune response but instead has been recruited for digestion of symbiotic bacteria in the stomach.

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

lysozyme activity

inferred from sequence or structural similarity

inferred from sequence or structural similarity

(Swiss-Prot Project Members, 1993.4.1)

non-traceable author statement

(Lemaitre, 2000.12.20)

non-traceable author statement

inferred from electronic annotation with InterPro:IPR000974

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

antimicrobial humoral response

non-traceable author statement

(Lemaitre, 2000.12.20)

cell wall catabolic process

inferred from electronic annotation with InterPro:IPR000974

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

extracellular region

inferred from electronic annotation with InterPro:IPR001916

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

LysP allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

63675

Orthologs

Genome-wide drosophilid orthologs

Dana\GF10070

Dere\GG14615

Dper\GL14512

Dpse\GA28484

Dvir\GJ12893

Dyak\GE20976

Curated drosophilid orthologs

Dpse\GA21554

InParanoid orthologs - Eukaryotic orthologs

•

ENSBTAP00000015846
Bos taurus (Ensembl)
ENSBTAP00000027399
Bos taurus (Ensembl)
ENSCAFP00000013141
Canis familiaris (Ensembl)
ENSRNOP00000007747
Rattus norvegicus (Ensembl)
MGI:96902
Mus musculus (Informatics)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v38139

w¹¹¹⁸; P{GD5896}v38139

v50537

w¹¹¹⁸; P{GD17546}v50537/TM3

Genomic Clones ( 1 )

BACR22D12

cDNA Clones ( 5 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo