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Gene Dmel\me31B

General Information
Symbol	Dmel\me31B	Species	D. melanogaster
Name	maternal expression at 31B	Annotation symbol	CG4916
Feature type	protein_coding_gene	FlyBase ID	FBgn0004419
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	31B1-31B1	Sequence location	2L:10,239,326..10,242,175 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene maternal expression at 31B is referred to in FlyBase by the symbol me31B (CG4916, FBgn0004419). It has the cytological map location 31B1. Its sequence location is 2L:10239326..10242175. Its molecular function is described as: ATP-dependent RNA helicase activity; RNA binding; ATP binding. The biological processes in which it is involved are not known. 13 alleles are reported. The phenotypes of these alleles are annotated with: egg chamber; germ cell; oocyte; ring canal. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31B1-31B1   Limits computationally determined from genome sequence between P{lacW}me31Bk06607 and P{PZ}nmd08774
Experimentally Determined Cytological Location
Cytogenetic map Notes References 31B1-31B2 (determined by in situ hybridisation)   (Spradling et al., 1999) 31B1-31B2   (BDGP Project Members, 1994-1999) 31B-31D (determined by in situ hybridisation)   (de Valoir et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: me31B+ chico- bsk- Ror+ (Riesgo-Escovar et al., 1996) In direction of increasing cytology: me31B+ chico- bsk- (Bohni et al., 1999) Gene Order (overall orientation not stated) References Overall orientation not stated: me31B+ bsk- (Sluss et al., 1996)
Gene Model & Products
Please see the GBrowse view of Dmel\me31B for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) me31B-RA FBtr0079975  NM_078809   1717   459 me31B-RB FBtr0079976  NM_164898   1603   428
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein me31B-PA   FBpp0079565   51.9   459   7.71     NP_523533   AAF52881 me31B-PB   FBpp0079566   48.6   428   8.06     NP_723539   AAN10728
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004419
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA helicase, ATP-dependent, DEAD-box, conserved site (IPR000629) DNA/RNA helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase, N-terminal (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014) Helicase, superfamily 1 and 2, ATP-binding (IPR014021)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390382   AA438292   AA538873   AA539562   AA541041   AA694682   AA696874   AA735399   AA735503   AA801980   AA802026   AA802445   AA821052   AA948818   AA948819   AA948820   AA949461   AA979222   AA979312   AA979637   AE003628 AAF52881   AE003628 AAN10728   AI455115   AI455726   AI512389   AI513031   AI513901   AI515514   AQ025801   AY051663 AAK93087   BG636274   BI163319   BI164959   BI165821   BI166711   BI168746   BI169188   BI170571   BI173498   BI213447   BI215117   BI215756   BI228684   BI230823   BI231450   BI236477   BI238811   BI243524   BI243977   BI353504   BI356093   BI356368   BI357466   BI357740   BI358455   BI361912   BI361993   BI363163   BI363736   BI363937   BI364810   BI365493   BI366035   BI366103   BI367047   BI367517   BI368191   BI368478   BI371057   BI371123   BI373867   BI375132   BI482179   BI486031   BI578214   BI578401   BI632755   BI639915   BQ102628   BQ102700   BQ102920   BQ102923   BQ103335   BQ103601   CA807156   CO264904   CO265980   CO276273   CO282734   CO296197   CO296727   CO307875   CO314628   CO321733   CO324879   CZ480825   CZ488994   EC055066   EC056390   EC066366   EC066735   EC072430   EC072987   EC076507   EC077599   EC082930   EC211684   EC224843   EC229827   EC233638   EC246796   M59926 AAA28603
UniProtKB/Swiss-Prot	P23128
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	GM01268 (Spradling et al., 1999) LD21247 (BDGP Project Members, 2000-) D1265 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References rescue fragment me31B[+t3.7] 2L:10,238,508..10,242,187 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=AflII-AflII_rfrag (Nakamura et al., 2001)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 7162
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004419 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004419 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004419
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable me31BCB05282 me31Bk06607 lethal | recessive me31Bk06607 lethal | larval stage | recessive me31BΔ1 me31BΔ2 me31BΔ3 Other Phenotypes Allele wild-type me31B+t3.7 me31B+t9 Phenotype manifest in Allele egg chamber | germ-line clone me31BΔ1 me31BΔ2 germ cell | germ-line clone me31BΔ1 me31BΔ2 nurse cell & nucleus | germ-line clone me31BΔ1 me31BΔ2 oocyte | germ-line clone me31BΔ1 me31BΔ2 ring canal | germ-line clone me31BΔ1 me31BΔ2
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of me31B Class Mutagen Stocks Known lesion me31BCB05282 P-element activity 0 Yes me31Be03055 piggyBac transposase 1 -- me31Bf06306 piggyBac transposase 1 -- me31Bk06607 P-element activity 2 Yes me31Bunspecified 0 -- me31BΔ1 Δ2-3 0 Yes me31BΔ2 Δ2-3 0 Yes me31BΔ3 Δ2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of me31B Class Mutagen Stocks Known lesion me31B+t3.7 in vitro construct | genomic fragment 0 Yes me31B+t9 in vitro construct | genomic fragment 0 Yes me31BGD11470 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes me31Bt5k in vitro construct | genomic fragment 0 Yes me31Bvas.T:Avic\GFP-EGFP in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)TE31Aa-1
Useful duplication	Dp(2;2)Mdh3
Not disrupted in	Df(2L)J2 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Disrupted in	Df(2L)Exel6026 (Ryder, 2004.4.26) Df(2L)J2 (Nakamura et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{me31B+3.7} NA P{me31B+9} NA P{me31Bt5k} NA P{vas-GFP-me31B.N} NA UAS construct P{GD11470} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}me31Be03055 NA PBac{WH}me31Bf06306 NA P{PTT-GB}me31BCB05282 NA insertion of enhancer trap P{lacW}me31Bk06607 No
Related Comments
	Please look at the allele reports for the complete phenotype data me31B has been cloned, sequenced and its expression pattern analysed. (de Valoir et al., 1991) During early oogenesis, loss of me31B function causes premature translation of oocyte-localising RNAs within nurse cells, without affecting their transport to the oocyte. (Nakamura et al., 2001)
Gene Ontology: Function, Process & Cellular Component ( 3 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000629, InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-)
ATP-dependent RNA helicase activity non-traceable author statement (Spradling et al., 1999) inferred from sequence or structural similarity with UniProt:P54824 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1991.11.1)
RNA binding inferred from direct assay (Chekulaeva et al., 2006)
Biological Process
CV term Evidence References
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions me31B allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 60455
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15754 Dere\GG10095 Dgri\GH13374 Dmoj\GI18279 Dper\GL19172 Dsec\GM18037 Dsim\GD23667 Dvir\GJ17531 Dwil\GK21209 Dyak\me31B
Curated drosophilid orthologs	Dpse\GA18523   Dyak\me31B
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000010638 Anopheles gambiae (Ensembl) ENSBTAP00000011952 Bos taurus (Ensembl) ENSCINP00000015240 Ciona intestinalis (Ensembl) NEWSINFRUP00000156719 Takifugu rubripes (Ensembl) ENSGALP00000012391 Gallus gallus (Ensembl) ENSP00000264018 Homo sapiens (Ensembl) ENSMMUP00000024532 Macaca mulatta (Ensembl) ENSMODP00000016726 Monodelphis domestica (Ensembl) ENSPTRP00000044660 Pan troglodytes (Ensembl) ENSRNOP00000017391 Rattus norvegicus (Ensembl) GSTENP00030222001 Tetraodon nigroviridis (Ensembl) ENSXETP00000022184 Xenopus tropicalis (Ensembl) Q6H7S2 Oryza sativa (Gramene) Q6H7S3 Oryza sativa (Gramene) AT2G45810.1 Arabidopsis thaliana (Arabidopsis) AT4G00660.1 Arabidopsis thaliana (Arabidopsis) WBGene00030815 Caenorhabditis briggsae (Wormbase) WBGene00000479 Caenorhabditis elegans (Wormbase) DDB0184074 Dictyostelium discoideum (Dictybase) MGI:104976 Mus musculus (Informatics) YDL160C Saccharomyces cerevisiae (Yeastgenome) SPBC776.09 Schizosaccharomyces pombe (Sanger)