A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\DNApol-γ35

General Information
SymbolDmel\DNApol-γ35SpeciesD. melanogaster
NameDNA polymerase γ 35kDAnnotation symbolCG33650
Feature typeprotein_coding_geneFlyBase IDFBgn0004407
Created / Updated2005-03-17/2005-03-17
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map34D1-34D1Sequence location2L:13,831,480..13,833,167 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene DNA polymerase γ 35kD is referred to in FlyBase by the symbol DNApol-γ35 (CG33650, FBgn0004407). It has the cytological map location 34D1. Its sequence location is 2L:13831480..13833167. Its molecular function is described as: DNA-directed DNA polymerase activity; ATP binding; glycine-tRNA ligase activity. It is involved in the biological processes: mitochondrial genome maintenance; mitochondrion organization and biogenesis; DNA-dependent DNA replication; mitochondrial DNA replication; glycyl-tRNA aminoacylation. 5 alleles are reported. The phenotypes of these alleles are annotated with: mitochondrion; larval central nervous system. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
34D1-34D1  
Limits computationally determined from genome sequence between P{lacW}Sosk05224&P{lacW}RpII33k05605 and P{lacW}l(2)34Fak00811&P{PZ}wb09437  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
34D-34E
 
(Kaguni, 1997.3.20)
Experimentally Determined Recombination Data
Location
Left of (cM)
(Ashburner et al., 1999)
Right of (cM)
Notes
Maps between Sop2 or Orc5 and RpII33.
(Ashburner et al., 1999)
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: Orc5+ DNApol-γ35- RpII33+
(Ashburner et al., 1999)
Gene Order (overall orientation not stated)
References
Overall orientation not stated: RpII33- DNApol-γ35+ Orc5- Sop2+ Orc5- Sop2+ tam+
(Lefai et al., 2000)
hide Gene Model & Products
Please see the GBrowse view of Dmel\DNApol-γ35 for information on other features
detailed view FBtr0080543 FBtr0080570 FBtr0080569 FBtr0091627 FBtr0091626 FBtr0080544 FBtr0080545 FBtr0080566 FBpp0080120 FBpp0099758 FBpp0088773 FBpp0080121 FBpp0080122 FBpp0080143 FBti0039326 FBti0006260 FBti0026321
Comments on Gene Model
gene_with_dicistronic_processed_transcript ; SO:0000722
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
DNApol-γ35-RA
FBtr0091627
 NM_001032091
  1634
  361
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
DNApol-γ35-PA  
FBpp0099758  
41.0  
361  
7.41  
  NP_001027262
  AAF53341
Additional Polypeptide Data & Comments
Reported size (kD)
35 (kD)
(Wernette et al., 1988, Kaguni et al., 1988, Wernette and Kaguni, 1986)
Comments
Mitochondrial DNA polymerase was purified to near homogeneity from early embryos. It is a heterodimer consisting of two subunits of 125kD and 35kD. The large subunit contains the DNA-polymerase activity.The DNA polymerase utilizes a large variety of template-primers efficiently.
(Wernette and Kaguni, 1986)
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Glycyl-tRNA synthetase, alpha2 dimer (IPR002315)
Anticodon-binding (IPR004154)
MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins
•
  • MTDROME08969
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA695573
 
AE003408
AAF44825
 
AE003641
AAF53341
 
AI106934
 
AI238599
 
AI297248
 
AI513318
 
AI514928
 
AY051491
AAK92915
 
BF488488
 
BF489699
 
BF489738
 
BF490117
 
BF490171
 
BF490439
 
BF498448
 
BF500952
 
BI354456
 
BI354458
 
BI357166
 
BI361464
 
BI363980
 
BI365536
 
BI481621
 
BI482461
 
BI482883
 
BI570289
 
BI604720
 
BI613659
 
BI635859
 
BI641805
 
CK658179
 
CL527895
 
CO268805
 
CO333367
 
DN154135
 
EC063156
 
EC205381
 
L39629
 
U94702
AAC47536
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    (FlyBase, 1992-, Ashburner et al., 1999.10.12)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    (Ashburner et al., 1999, Ashburner et al., 1999.10.12)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    DNApol-γ35[1]
    2L:13,832,537..13,832,537
    evidence=experimental
    na_change=G13832537A
    pr_change=G31E|DNApol-gamma35-PA
    reported_na_change=G92A
    reported_pr_change=G31E
    (Iyengar et al., 2002)
    sequence variant
    DNApol-γ35[2]
    2L:13,832,278..13,832,278
    comment=A 74bp insertion within the T117 codon introduces a frameshift and a premature stop codon.
    evidence=experimental
    (Iyengar et al., 2002)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal | recessive
        lethal | pupal stage
        lethal | pupal stage (with Df(2L)b80c1)
        lethal | pupal stage (with DNApol-γ351)
        lethal | pupal stage (with DNApol-γ352)
        Other Phenotypes
        Allele
        locomotor behavior defective (with Df(2L)b80c1)
        neuroanatomy defective (with Df(2L)b80c1)
        neuroanatomy defective (with DNApol-γ351)
        neuroanatomy defective (with DNApol-γ352)
        Phenotype manifest in
        Allele
        mitochondrion (with Df(2L)b80c1)
        larval central nervous system (with Df(2L)b80c1)
        mitochondrion (with DNApol-γ352)
        larval central nervous system (with DNApol-γ352)
        mitochondrion (with DNApol-γ351)
        larval central nervous system (with DNApol-γ351)
        hide Classical Alleles ( 4 )
        For All Classical Alleles Show

        Allele of DNApol-γ35ClassMutagenStocksKnown lesion
        DNApol-γ3512 Yes
        DNApol-γ35210 Yes
        DNApol-γ3530 --
        DNApol-γ35EY063661 Yes
        hide Alleles Carried on Transgenic Constructs ( 1 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of DNApol-γ35ClassMutagenStocksKnown lesion
        DNApol-γ35hs.PI0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Useful duplication
         
        Disrupted in
        (Iyengar et al., 1999, Woodruff and Ashburner, 1979)
        (Gubb et al., 1990)
        (Roote et al., 1996)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Woodruff and Ashburner, 1979, Gubb et al., 1990)
        (Iyengar et al., 1999, Iyengar et al., 2002, Ashburner et al., 1982)
        (Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Iyengar et al., 1999, Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Roote, 2000.6.13)
        (Roote, 2000.6.13)
        (Roote, 1997.6.3)
        (Roote, 1998.5.15, Ashburner et al., 1999, Roote, 2000.6.5)
        (Roote, 1997.6.3)
        Not disrupted in
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982, Gubb et al., 1990, Roote, 1996.8, Roote, 1997.6.3)
        (Ashburner et al., 1982, Gubb et al., 1990)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Roote, 1997.6.3)
        (Gubb et al., 1990)
        (Woodruff and Ashburner, 1979)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Roote, 1997.6.3)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1985)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Gubb et al., 1984)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Roote, 1997.6.3)
        (Roote, 1997.6.3)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979, Ashburner et al., 1982)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Gubb et al., 1985)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Woodruff and Ashburner, 1979)
        (Ashburner et al., 1982)
        Not duplicated in
        (Roote, 1997.6.3)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        heat-shock construct
        P{HS-DNApol-γ35.I}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        insertion of mobile activating element
        P{EPgy2}CG33649EY06366
        NA
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        DNApol-γ125 and DNApol-γ35 have been isolated and purified and their subunit structure and catalytic properties examined.
        (Wernette and Kaguni, 1986)
        Comparison of the rate and specificity of DNA synthesis by DNApol-γ125 and DNApol-γ35 on several template primers, and an examination of the products of DNA synthesis has been carried out.
        (Wernette et al., 1988)
        The 3' to 5' exonuclease encoded provides a proof reading function to enhance the fidelity of DNA synthesis during mitochondrial DNA replication.
        (Kaguni and Olson, 1989)
        A structural and mechanistic analysis of DNApol-γ125 and DNApol-γ35 demonstrates them to be both effective and accurate in the synthesis of DNA.
        (Kaguni et al., 1988)
        The rate and processivity of DNA synthesis by DNA polymerase γ has been analysed under a variety of different conditions.
        (Williams et al., 1993)
        mtSSB stimulates DNA polymerase γ activity in vitro.
        (Williams and Kaguni, 1995)
        Subunit structure and enzymatic activity of DNA polymerase γ are studied using a combination of physical and immunological approaches. The role of the 2 subunits in template primer DNA binding is explored.
        (Olson et al., 1995)
        hide Gene Ontology: Function, Process & Cellular Component ( 10 )
        hide Molecular Function
        CV term
        Evidence
        References
        ATP binding
        inferred from electronic annotation with InterPro:IPR002315, InterPro:IPR004154
        (FlyBase Curators et al., 2004-)
        DNA-directed DNA polymerase activity
        non-traceable author statement
        (Kaguni, 1997.3.20)
        inferred from sequence or structural similarity
        (Ashburner et al., 1999)
        inferred from sequence or structural similarity with EMBL:AF006072
        (FlyBase Genome Annotators, 2002-2003)
        inferred from physical interaction with FLYBASE:tam; FB:FBgn0004406
        (Wang et al., 1997)
        inferred from sequence or structural similarity with UniProtKB:Q9UHN1
        (Sardiello et al., 2003.6.11)
        glycine-tRNA ligase activity
        inferred from electronic annotation with InterPro:IPR002315
        (FlyBase Curators et al., 2004-)
        hide Biological Process
        CV term
        Evidence
        References
        DNA-dependent DNA replication
        inferred from physical interaction with FLYBASE:tam; FB:FBgn0004406
        (Wang et al., 1997)
        inferred from sequence or structural similarity with UniProtKB:Q9UHN1
        (Sardiello et al., 2003.6.11)
        glycyl-tRNA aminoacylation
        inferred from electronic annotation with InterPro:IPR002315
        (FlyBase Curators et al., 2004-)
        mitochondrial DNA replication
        traceable author statement
        (Garesse and Vallejo, 2001)
        mitochondrial genome maintenance
        inferred from mutant phenotype
        (Iyengar et al., 2002)
        mitochondrion organization and biogenesis
        inferred from mutant phenotype
        (Iyengar et al., 2002)
        hide Cellular Component
        CV term
        Evidence
        References
        gamma DNA polymerase complex
        inferred from sequence or structural similarity
        (Ashburner et al., 1999)
        inferred from sequence or structural similarity with EMBL:AF006072
        (FlyBase Genome Annotators, 2002-2003)
        inferred from physical interaction with FLYBASE:tam; FB:FBgn0004406
        (Wang et al., 1997)
        mitochondrion
        inferred from sequence or structural similarity with EMBL:AF006072
        (FlyBase Genome Annotators, 2002-2003)
        inferred from physical interaction with FLYBASE:tam; FB:FBgn0004406
        (Wang et al., 1997)
        inferred from sequence or structural similarity with UniProtKB:Q9UHN1
        (Sardiello et al., 2003.6.11)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        gene_with_dicistronic_mRNA
        (FlyBase Genome Annotators, 2002-2003)
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        DNApol-γ35 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •
        hide Orthologs
        Genome-wide drosophilid orthologs
        Curated drosophilid orthologs
         
        Linkouts
        InParanoid orthologs - Eukaryotic orthologs
        •
        hide Functional Complementation between Species
        hide Inter-Species Misexpression Data
        Produces phenotype in
        Produces NO phenotype in
        hide Stocks & Reagents