A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\CrebA

General Information
SymbolDmel\CrebASpeciesD. melanogaster
NameCyclic-AMP response element binding protein AAnnotation symbolCG7450
Feature typeprotein_coding_geneFlyBase IDFBgn0004396
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map71E1-71E1Sequence location3L:15,528,514..15,546,314 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Cyclic-AMP response element binding protein A is referred to in FlyBase by the symbol CrebA (CG7450, FBgn0004396). It has the cytological map location 71E1. Its sequence location is 3L:15528514..15546314. Its molecular function is described as: DNA binding; RNA polymerase II transcription factor activity; protein homodimerization activity; transcription factor activity; sequence-specific DNA binding; protein dimerization activity. It is involved in the biological processes: regulation of transcription from RNA polymerase II promoter; dorsal/ventral pattern formation; chitin-based larval cuticle pattern formation; salivary gland development; salivary gland morphogenesis; larval chitin-based cuticle development; transcription from RNA polymerase II promoter. 22 alleles are reported. The phenotypes of these alleles are annotated with: larva; larval cuticle; ommatidium; eye photoreceptor cell; eye. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
71E1-71E1  
Limits computationally determined from genome sequence between P{lacW}cp309s2172&P{lacW}l(3)j2A2j2A2 and P{EP}Tfb2EP572  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
71D1-71D2
(determined by in situ hybridisation)  
(Spradling et al., 1999)
71D1-71D2
(determined by in situ hybridisation)  
(BDGP Project Members, 1994-1999)
25C-25D
(determined by in situ hybridisation)  
(Abel et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\CrebA for information on other features
detailed view FBtr0075557 FBtr0075558 FBtr0075559 FBtr0075560 FBpp0089339 FBpp0075311 FBpp0075312 FBpp0075313 FBti0069135 FBti0100026 FBti0055738 FBti0069144 FBti0070778 FBti0068224 FBti0052248 FBti0004124 FBti0052789 FBti0057949 FBti0050194 FBti0011562 FBti0039187 FBti0035137 FBti0035919
Comments on Gene Model
DGC clone appears problematic (SD05937): polyA-primed genomic contaminant
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
CrebA-RA
FBtr0075557
 NM_079363
  4410
  516
CrebA-RB
FBtr0075558
 NM_206374
  4479
  516
Additional Transcript Data & Comments
Reported size (kB)
4.3 (northern blot)
(Abel et al., 1992)
4.4 (northern blot)
(Smolik-Utlaut et al., 1992)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
CrebA-PA  
FBpp0075311  
56.3  
516  
6.31  
  NP_524087
  AAF49621
CrebA-PB  
FBpp0089339  
56.3  
516  
6.31  
  NP_996096
  AAS64997
Additional Polypeptide Data & Comments
Reported size (kD)
516 (aa); 56.3 (kD)
(Smolik-Utlaut et al., 1992)
515 (aa); 56 (kD)
(Abel et al., 1992)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
cAMP response element binding (CREB) protein (IPR001630)
Basic-leucine zipper (bZIP) transcription factor (IPR004827)
Eukaryotic transcription factor, Skn-1-like, DNA-binding (IPR008917)
bZIP transcription factor, bZIP-1 (IPR011616)
TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles
•
  • T01603
  • T01604
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA141154
 
AE003530
AAF49621
 
AE003530
AAS64997
 
AI541662
 
AQ034057
 
AW944445
 
AY122251
AAM52763
 
BG636528
 
BI355920
 
BI368443
 
BI639496
 
BP552852
 
BP552853
 
BT011074
 
BT021243
AAX33391
 
BT025219
 
CL943677
 
CO182767
 
CO188599
 
CO189362
 
CO189616
 
CO189866
 
CO269515
 
CO276581
 
CO276987
 
CO282341
 
CO294337
 
CO299017
 
CO319198
 
CO325755
 
CO333808
 
CO338617
 
CO340292
 
CO344187
 
CZ484246
 
CZ484247
 
CZ487823
 
CZ488815
 
CZ489984
 
DN154078
 
EC060659
 
EC061018
 
EC064526
 
EC071209
 
EC072503
 
EC074992
 
EC083118
 
EC090619
 
EC090831
 
EC195291
 
EC197679
 
EC199176
 
EC200760
 
EC201347
 
EC201475
 
EC206089
 
EC215576
 
EC216020
 
EC218157
 
EC218213
 
EC222293
 
EC222473
 
EC235925
 
EC238216
 
EC241262
 
EC253342
 
EC258551
 
G00569
 
M87038
AAA28427
 
X64429
CAA45771
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
(BDGP Project Members, 2000-)
(BDGP Project Members, 1994-1999, Spradling et al., 1999)
(BDGP Project Members, 1994-1999)
hide Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
DEDB - Drosophila exon database: splicing graphs
•
Crossreferences
hide Expression Data
FlyBase-Curated Data
Transcript and
Protein data 		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
hide Summary of Transcript Expression
Stage
Tissue/Position
Reference
 adult stage | male
 ejaculatory duct
(Smolik-Utlaut et al., 1992)
 adult stage
 adult midgut
(Smolik-Utlaut et al., 1992)
 adult stage | male
 ejaculatory bulb
(Smolik-Utlaut et al., 1992)
 larval stage | third instar
 embryonic/larval digestive system
(Abel et al., 1992)
 adult stage | male
 seminal vesicle
(Smolik-Utlaut et al., 1992)
 embryonic stage
 embryonic/larval salivary gland
(Smolik-Utlaut et al., 1992)
 larval stage | third instar
 embryonic/larval fat body
(Abel et al., 1992)
 larval stage | third instar
 embryonic/larval trachea
(Abel et al., 1992)
 oogenesis stage,adult stage
 follicle cell
(Smolik-Utlaut et al., 1992)
 adult stage
 adult brain
(Smolik-Utlaut et al., 1992)
 adult stage
 optic lobe
(Smolik-Utlaut et al., 1992)
 adult stage
 adult salivary gland
(Smolik-Utlaut et al., 1992)
 embryonic stage-adult stage
 
(Abel et al., 1992)
 adult stage
 cardia
(Smolik-Utlaut et al., 1992)
Marker for
    Subcellular Localization
    CV Term
    hide Summary of Polypeptide Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage | stage 11-12
     embryonic salivary gland
    (Bradley et al., 2003)
     oogenesis stage,adult stage | female
     ovary
    (Abel et al., 1992)
    Marker for
    Subcellular Localization
    CV Term
    nucleus
    (Abel et al., 1992)
    hide External Data & Images
    Linkouts
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    •
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    •
    GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
    •
    FlyExpress - Embryonic expression images (BDGP data)
    •
    Show all FlyExpress processed images for CrebA
    hide Alleles & Phenotypes
    hide Summary of Allele Phenotypes
    Lethality
    Allele
    viable
    lethal | recessive
    lethal | embryonic stage | recessive
    lethal | larval stage | recessive
    lethal, with Scer\GAL432B
    lethal, with Scer\GAL4h-1J3
    lethal, with Scer\GAL435
    lethal, with Scer\GAL4323
    lethal, with Scer\GAL4202
    lethal, with Scer\GAL4128
    semi-lethal
    Other Phenotypes
    Allele
    visible | somatic clone
    visible, with Scer\GAL4GMR.PF
    visible, with Scer\GAL4ey.PH
    Sterility
    Allele
    fertile
    Phenotype manifest in
    Allele
    larva
    larval cuticle
    denticle belt & larva
    ommatidium, with Scer\GAL4GMR.PF
    ommatidium, with Scer\GAL4ey.PH
    eye | ectopic, with Scer\GAL4ey.PH
    ommatidium | somatic clone
    eye photoreceptor cell | somatic clone
    hide Classical Alleles ( 20 )
    For All Classical Alleles Show

    Allele of CrebAClassMutagenStocksKnown lesion
    CrebA03576loss of function1 Yes
    CrebAEY134941 --
    CrebAEY151241 --
    CrebAf017731 --
    CrebAf051981 --
    CrebAf061651 --
    CrebAf071840 --
    CrebArR3-40 Yes
    CrebArR34
      		0 Yes
      CrebArR5loss of function0 Yes
      CrebArR7loss of function0 Yes
      CrebArR90 Yes
      CrebAunspecified
        		0 --
        CrebAwR11loss of function0 Yes
        CrebAwR13
          		0 Yes
          CrebAwR23loss of function0 Yes
          CrebAwR3
            		0 Yes
            CrebAwR7
              		0 Yes
              CrebAwR83loss of function0 Yes
              CrebAwR84loss of function0 Yes
              hide Alleles Carried on Transgenic Constructs ( 2 )
              For All Alleles Carried on Transgenic Constructs Show

              Allele of CrebAClassMutagenStocksKnown lesion
              CrebAGD17250 Yes
              CrebAScer\UAS.cRa0 Yes
              hide Aneuploid Aberrations
              Useful deficiency
               
              Useful duplication
               
              Disrupted in
              (Andrew et al., 1997, Rose et al., 1997)
              (Christensen et al., 2008.4.15)
              (Spradling et al., 1999, BDGP Project Members, 1994-1999)
              (Rose et al., 1997)
              (Rose et al., 1997)
              (Rose et al., 1997)
              (Spradling et al., 1999, BDGP Project Members, 1994-1999)
              (Rose et al., 1997)
              (Rose et al., 1997)
              Not disrupted in
              (Rose et al., 1997)
              hide Transgenic Constructs & Insertions
              Transgenic Constructs
              Type of construct
              Name
              Expression data
              reporter construct
              P{CrebA-lacZ.0.77}
              No
              P{CrebA-lacZ.1.1.fkh1-6}
              No
              P{CrebA-lacZ.1.1}
              No
              P{CrebA-lacZ.2.8}
              No
              UAS construct
              P{GD1725}
              NA
              P{UAS-CrebA.R}
              NA
              Insertions
              Type of insertions
              Name
              Expression data
              miscellaneous insertions
              PBac{WH}CrebAf01773
              NA
              PBac{WH}CrebAf05198
              NA
              PBac{WH}CrebAf06165
              NA
              PBac{WH}CrebAf07184
              NA
              P{?'PZ}CrebArR9
              NA
              P{?'lacW}CrebAwR83
              NA
              P{GT1}CG13453BG01366
              NA
              P{PZ-ΔrR3-4}CrebArR3-4
              NA
              P{PZ-ΔrR5}CrebArR5
              NA
              P{PZ-ΔrR7}CrebArR7
              NA
              insertion of enhancer trap
              P{PZ}CrebA03576
              Yes
              P{lacW}B204
              Yes
              insertion of mobile activating element
              P{EPgy2}CrebAEY13494
              NA
              P{EPgy2}CrebAEY15124
              NA
              hide Related Comments
              Please look at the allele reports for the complete phenotype data
              Isolation and characterization of Creb reveals that the protein binds to the CRE sequence and belongs to the leucine zipper family of transcription factors.
              (Smolik-Utlaut et al., 1992)
              CrebA mutants display lateralisation of the larval cuticle and the cuticle is significantly weaker than wild type larval cuticle. The salivary gland and tracheae display a 'crooked' phenotype.
              (Andrew et al., 1995)
              In the epidermis CrebA is required for patterning cuticular structures on both the dorsal and ventral surfaces of larvae, mutant larvae have only lateral structures around the entire circumference of each segment. Epistasis tests with known dorsal/ventral patterning genes suggests that CrebA encodes a transcription factor near the end of both the dpp and spi signalling cascades to translate the corresponding extracellular signals into changes in gene expression. Double mutants with segment polarity genes confirm that CrebA is not involved in segment polarity.
              (Andrew et al., 1997)
              hide Gene Ontology: Function, Process & Cellular Component ( 14 )
              hide Molecular Function
              CV term
              Evidence
              References
              DNA binding
              inferred from direct assay
              (Abel et al., 1992)
              inferred from direct assay
              (Smolik-Utlaut et al., 1992)
              non-traceable author statement
              (Swiss-Prot Project Members, 1993.4.1)
              RNA polymerase II transcription factor activity
              inferred from direct assay
              (Smolik-Utlaut et al., 1992)
              protein dimerization activity
              inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616
              (FlyBase Curators et al., 2004-)
              protein homodimerization activity
              inferred from sequence or structural similarity
              (Fassler et al., 2002)
              sequence-specific DNA binding
              inferred from electronic annotation with InterPro:IPR004827, InterPro:IPR011616
              (FlyBase Curators et al., 2004-)
              transcription factor activity
              non-traceable author statement
              (Swiss-Prot Project Members, 1993.4.1)
              inferred from electronic annotation with InterPro:IPR001630, InterPro:IPR004827, InterPro:IPR011616
              (FlyBase Curators et al., 2004-)
              hide Biological Process
              CV term
              Evidence
              References
              chitin-based larval cuticle pattern formation
              inferred from mutant phenotype
              (Andrew et al., 1997)
              inferred from mutant phenotype
              (Abrams and Andrew, 2005)
              dorsal/ventral pattern formation
              inferred from genetic interaction with FLYBASE:dpp; FB:FBgn0000490
              (Andrew et al., 1997)
              larval chitin-based cuticle development
              inferred from mutant phenotype
              (Abrams and Andrew, 2005)
              regulation of transcription from RNA polymerase II promoter
              inferred from direct assay
              (Smolik-Utlaut et al., 1992)
              salivary gland development
              inferred from mutant phenotype
              (Andrew et al., 1997)
              traceable author statement
              (Abrams et al., 2003)
              salivary gland morphogenesis
              non-traceable author statement
              (Bradley et al., 2001)
              transcription from RNA polymerase II promoter