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Gene Dmel\Klp67A

General Information
Symbol	Dmel\Klp67A	Species	D. melanogaster
Name	Kinesin-like protein at 67A	Annotation symbol	CG10923
Feature type	protein_coding_gene	FlyBase ID	FBgn0004379
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67B2-67B2	Sequence location	3L:9,355,731..9,360,040 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Kinesin-like protein at 67A is referred to in FlyBase by the symbol Klp67A (CG10923, FBgn0004379). It has the cytological map location 67B2. Its sequence location is 3L:9355731..9360040. Its molecular function is described as: motor activity; microtubule motor activity; ATP binding. It is involved in the biological processes: microtubule-based movement; centrosome separation; chromosome segregation; cytokinesis; microtubule polymerization; spindle assembly; male meiosis chromosome segregation; male meiotic chromosome movement towards spindle pole; sister chromatid biorientation; mitotic spindle organization and biogenesis. 8 alleles are reported. The phenotypes of these alleles are annotated with: spindle microtubule; astral microtubule; spindle pole; S2 cell-line; spindle; meiosis I; meiosis II; cytoplasmic microtubule. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67B2-67B2   Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
Experimentally Determined Cytological Location
Cytogenetic map Notes References 67B1-67B5 (determined by in situ hybridisation)   (Deak et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Klp67A for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Klp67A-RA FBtr0076499  NM_079268   2893   814
Additional Transcript Data & Comments
Reported size (kB)	3.0, 2.8 (northern blot) (Stewart et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Klp67A-PA   FBpp0076227   92.4   814   9.67     NP_523992   AAF50296
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004379
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Kinesin, motor region (IPR001752)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264943   AA735387   AA941962   AE003552 AAF50296   AI113659   AI518034   AQ072986   AX154875   AX260158   AY071433 AAL49055   BF496916   BG638338   BG638499   BI167013   BI229799   BI232562   BI366470   BI366522   BI368072   CK605524   CK605525   CK659108   CO190083   CO282501   EC073539   EC226382   EL884513   EL884514   EL884637   EL884638   EL884746   LD27326   M74429 AAA28656   U89264 AAB49460   UT01992
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	P91945 Q00084 Q9VSW5
Maps to
Does NOT map to
Identified with	RE52076 (BDGP Project Members, 2000-, Goshima and Vale, 2005)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 4661
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  adult stage | male  testis (Stewart et al., 1991)  adult stage | female  ovary (Stewart et al., 1991)  embryonic stage,larval stage,pupal stage   (Stewart et al., 1991)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA941962
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004379 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004379 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004379 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Klp67A Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | recessive Klp67AS042705 Other Phenotypes Allele meiotic cell cycle defective | male Klp67AS042705 meiotic cell cycle defective | male (with Df(3L)29A6) Klp67A322b24 meiotic cell cycle defective | male, with Scer\GAL4[-]: Klp67AEP3516 mitotic cell cycle defective (with Df(3L)29A6) Klp67A322b24 cytokinesis defective | male (with Df(3L)29A6) Klp67A322b24 Sterility Allele female semi-sterile (with Df(3L)29A6) Klp67A322b24 male sterile (with Df(3L)29A6) Klp67A322b24 Phenotype manifest in Allele meiosis I | male (with Df(3L)29A6) Klp67A322b24 meiosis II | male (with Df(3L)29A6) Klp67A322b24 spermatocyte & aster | ectopic (with Df(3L)29A6) Klp67A322b24 spermatocyte & astral microtubule (with Df(3L)29A6) Klp67A322b24 spermatocyte & spindle | supernumerary (with Df(3L)29A6) Klp67A322b24 spermatocyte & contractile ring (with Df(3L)29A6) Klp67A322b24 spermatid & Nebenkern (with Df(3L)29A6) Klp67A322b24 spermatid & nucleus (with Df(3L)29A6) Klp67A322b24 spermatocyte & centrosome | supernumerary (with Df(3L)29A6) Klp67A322b24 spermatocyte & condensed nuclear chromosome (with Df(3L)29A6) Klp67A322b24 centrosome & blastoderm embryo | maternal effect (with Df(3L)29A6) Klp67A322b24 spindle & blastoderm embryo | maternal effect (with Df(3L)29A6) Klp67A322b24 mitotic anaphase B & blastoderm embryo | maternal effect (with Df(3L)29A6) Klp67A322b24 mitotic metaphase & blastoderm embryo | maternal effect (with Df(3L)29A6) Klp67A322b24 spindle microtubule & blastoderm embryo | maternal effect (with Df(3L)29A6) Klp67A322b24 spermatocyte & nuclear chromosome (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4[-]: Klp67AEP3516 spindle Klp67AMtnA.T:Avic\GFP Klp67AdsRNA.cGa spermatocyte & nuclear chromosome (with Df(3L)29A6) Klp67AS042705 mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6) Klp67AS042705 meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6) Klp67AS042705 spindle microtubule Klp67AdsRNA.cGa astral microtubule Klp67AdsRNA.cGa mitosis & nuclear chromosome Klp67AdsRNA.cGa spindle pole Klp67AdsRNA.cGa S2 cell-line Klp67AdsRNA.cGa Klp67AMtnA.NES.T:Avic\GFP cytoplasmic microtubule Klp67AMtnA.NES.T:Avic\GFP
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Klp67A Class Mutagen Stocks Known lesion Klp67A322b24 Δ2-3 0 Yes Klp67AEP3516 P-element activity 2 -- Klp67AS042705 Δ2-3 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Klp67A Class Mutagen Stocks Known lesion Klp67AGD7127 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Klp67AMtnA.NES.T:Avic\GFP in vitro construct | coding region fusion 0 Yes Klp67AMtnA.T:Avic\GFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes Klp67AUbi-p63E.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes Klp67AdsRNA.cGa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)AC1
Useful duplication	Dp(3;3)M67C+2
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7127} NA characterization construct P{Ubi-Klp67A.MYC} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Klp67AS042705 No insertion of mobile activating element P{EP}Klp67A322b24 NA P{EP}Klp67AEP3516 NA
Related Comments
	Please look at the allele reports for the complete phenotype data Klp67A has been cloned, partially sequenced and its expression pattern analysed. (Stewart et al., 1991) Klp67A is a mitotic motor that may have a unique role of positioning mitochondria near the spindle. (Pereira et al., 1997) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: cells become arrested in metaphase and contain abnormally elongated, curved spindles which cause the entire cell to assume an elongated shape and exclude mitochondria from the cell poles. Additionally, metaphase chromosomes show an abnormal configuration. (Gandhi et al., 2004) The majority of post-meiotic Klp67A mutant spermatids contain two nuclei, which vary in size. During meiosis I, mutant primary spermatocytes have an increased number of microtubules throughout the cell and lack a distinct central spindle. Additionally, the k-fibres that link the chromosomes to the spindle are bent and wavy, instead of being straight, as in wild type. (Savoian et al., 2004) S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show an expansion of the metaphase spindle. Sometimes RNAi of this gene show centrosome detachment from the kinetochore microtubules. (Goshima et al., 2005) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and an aberrantly long spindle that is monastral and bipolar when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
Gene Ontology: Function, Process & Cellular Component ( 17 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001752 (FlyBase Curators et al., 2004-)
microtubule motor activity inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-)
motor activity inferred from direct assay AND inferred from sequence or structural similarity (Pereira et al., 1997)
Biological Process
CV term Evidence References
centrosome separation inferred from mutant phenotype (Gandhi et al., 2004)
chromosome segregation inferred from mutant phenotype (Gandhi et al., 2004)
cytokinesis inferred from mutant phenotype (Gandhi et al., 2004)
male meiosis chromosome segregation inferred from mutant phenotype (Savoian et al., 2004)
male meiotic chromosome movement towards spindle pole inferred from mutant phenotype (Savoian et al., 2004)
microtubule polymerization inferred from mutant phenotype (Gandhi et al., 2004)
microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-)
mitotic spindle organization and biogenesis inferred from mutant phenotype (Goshima et al., 2007)
sister chromatid biorientation inferred from mutant phenotype (Savoian et al., 2004)
spindle assembly inferred from mutant phenotype (Gandhi et al., 2004) inferred from mutant phenotype (Savoian et al., 2004)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Pereira et al., 1997)
kinesin complex traceable author statement (Pereira et al., 1997) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P53086 (FlyBase, 1992-)
microtubule associated complex inferred from direct assay (Pereira et al., 1997)
nucleus inferred from direct assay (Savoian et al., 2004)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Klp67A allele Gene References unspecified Klp61F (Gandhi et al., 2004) msps (Goshima et al., 2005) chb (Goshima et al., 2005)
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 64465
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23742 Dere\GG14050 Dgri\GH16987 Dmoj\GI11399 Dper\GL22636 Dpse\GA10646 Dsec\GM24884 Dsim\GD12936 Dvir\GJ13594 Dwil\GK16568 Dyak\GE21253
Curated drosophilid orthologs	Dpse\GA10646
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSBTAP00000002735 Bos taurus (Ensembl) ENSCAFP00000015029 Canis familiaris (Ensembl) NEWSINFRUP00000132262 Takifugu rubripes (Ensembl) ENSGALP00000019833 Gallus gallus (Ensembl) ENSP00000263181 Homo sapiens (Ensembl) ENSMMUP00000017901 Macaca mulatta (Ensembl) ENSMODP00000011838 Monodelphis domestica (Ensembl) ENSPTRP00000006013 Pan troglodytes (Ensembl) GSTENP00017870001 Tetraodon nigroviridis (Ensembl) ENSXETP00000009326 Xenopus tropicalis (Ensembl) AT1G18550.1 Arabidopsis thaliana (Arabidopsis) ZDB-CDNA-040425-1790 Danio rerio (Zfin) ZDB-GENE-040426-862 Danio rerio (Zfin) MGI:2446977 Mus musculus (Informatics) YGL216W Saccharomyces cerevisiae (Yeastgenome) SPBC2F12.13 Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Szeged	- P{EP}Klp67AEP3516 685 y1 w1; P{lacW}Klp67AS042705/TM3, Sb1 Ser1
VDRC	v51703 w1118 P{GD7127}v51703 v52105 w1118; P{GD7127}v52105
Bloomington	17269 w1118; P{EP}Klp67AEP3516/TM6B, Tb1
Genomic Clones ( 2 )
	BACR48N08 BACR01E20
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE52076
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD37437 LD08653 GH10062 RE07280 RE27657 LD21225 RE53982 RE28647 RE52007 LP16615
Other clones	UT01992 EK046430 LD27326 IP15894 AT10924 EK160617 IP15794 LD22887 IP15994
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004379 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004379
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: Klp67A CG10923 (Milshina, 1999.11)
Source for database merge of	Source for merge of: Klp67A anon-WO0140519.224 anon-WO0172774.120 Source for merge of Klp67A anon-WO0140519.224 anon-WO0172774.120 was sequence comparison (date:051113). (FlyBase, 1996-) Source for merge of: Klp67A l(3)S042705 (Savoian et al., 2004)
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079268 RefSeq (Proteins) • NP_523992 DDBJ / EMBL / GenBank • AE003552 AAF50296 AQ072986 AX154875 AX260158 AY071433