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Gene Dmel\neb

General Information
Symbol	Dmel\neb	Species	D. melanogaster
Name	nebbish	Annotation symbol	CG10718
Feature type	protein_coding_gene	FlyBase ID	FBgn0004374
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map	2-54.5
Cytogenetic map	38B3-38B5	Sequence location	2L:20,075,756..20,089,706 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nebbish is referred to in FlyBase by the symbol neb (CG10718, FBgn0004374). It has the cytological map location 38B3-38B5. Its sequence location is 2L:20075756..20089706. Its molecular function is described as: protein phosphatase 1 binding; microtubule motor activity; chromatin binding; plus-end-directed microtubule motor activity; ATP binding. It is involved in the biological processes: mitotic chromosome condensation; ovarian follicle cell development; meiosis; mitotic cell cycle, embryonic; microtubule-based movement; chromosome segregation; oogenesis; regulation of cell cycle. 39 alleles are reported. The phenotypes of these alleles are annotated with 28 unique terms, many of which group under: organ system; peripheral nervous system; nervous system; anatomical structure; embryonic abdomen; embryonic tagma; blastoderm embryo; adult segment; nuclear part; embryonic head; spermatozoon; cytoplasmic part; adult external prothorax; egg; syncytial blastoderm embryo. It has one annotated transcript and one annotated polypeptide.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	neb: nebbish (M. Fuller) Recessive late lethal. Some homozygotes survive until the pupal period, but very few eclose. Squash preparations of pupal testes reveal that the mitochondrial derivative (nebenkern) fails to elongate during spermatid differentiation. Flagellar axonemes are assembled. Onion stage early spermatids appear normal.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38B3-38B5   Limits computationally determined from genome sequence between P{lacW}barrk14014 and P{EP}nebEP642
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38B4-38B6   (Butler, 2001) 38B-38B   (Ohkura, 1997.1.21) 38B4-38B6 (determined by in situ hybridisation)   (Spradling et al., 1999) 38B-38B (determined by in situ hybridisation)   (Alphey et al., 1997) 38B-38B (determined by in situ hybridisation)   (Ohkura et al., 1997) 38B-38B   (Ruden et al., 1997) 38B3-38B4 (determined by in situ hybridisation)   (Bhat et al., 1996) 38B-38B (determined by in situ hybridisation)   (Ruden and Jaeckle, 1995) 38B1-38B2 38B3--4 38B3--5 38B3--6 38B4--5 38B4--6   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-54.5 (Molina et al., 1997, Molina, 1997.10.23) 2- (Ruden et al., 1997)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: barr- neb+ (Bhat et al., 1996) In direction of increasing cytology: pr- neb+ fok- neb+ (Ohkura et al., 1997) In direction of increasing cytology: neb+ fok- neb+ (Molina et al., 1997) In direction of increasing cytology: pr- neb+ fok- neb+ CG10747- (Butler et al., 2001) Gene Order (overall orientation not stated) References Overall orientation not stated: pr- neb+ fok- neb+ (Ruden et al., 1997)
Gene Model & Products
Please see the GBrowse view of Dmel\neb for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) neb-RA FBtr0081332  NM_057469   3601   1121
Additional Transcript Data & Comments
Reported size (kB)	3.6 (longest cDNA) (Alphey et al., 1997) 3.5 (northern blot) (Ohkura et al., 1997) 3.6 (northern blot) (Molina et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein neb-PA   FBpp0080864   125.2   1121   7.72     NP_476817   AAF53872
Additional Polypeptide Data & Comments
Reported size (kD)	1121 (aa); 110 (kD observed) (Ruden et al., 1997) 1121 (aa) (Molina et al., 1997) 1121 (aa); 125 (kD predicted) (Alphey et al., 1997, Ohkura et al., 1997)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004374
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (IPR000253) Kinesin, motor region (IPR001752) SMAD/FHA domain (IPR008984)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC004364   AC093044   AE003665 AAF53872   AF022650 AAB81511   AI532517   AI945699   AI946724   AQ025838   AQ254805   AW944341   AY052090 AAK93514   BF487804   BF496090   BG634409   BH023781   BH840626   BI640786   BT031282   CA805039   CA805790   CA806805   CG10718   CL844407   CO190346   CO193204   CO194939   CO317291   CO317319   CO318183   CO333328   CZ466658   CZ468115   CZ478440   EC057289   EC060512   EC206955   EC250009   G00600   X99617 CAA67928   Y10667 CAA71675   Y15247 CAA75531
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O16866 O18390 Q7JMZ1 Q7KQ58 Q960F4 Q9VIP4
Maps to	DS00863 (FlyBase, 1992-, Butler et al., 2001) FBcl0297594 (FlyBase, 1992-)
Does NOT map to
Identified with	bs30a04 bs15g10 (Oliver, 2001.8.16) SD04054 (BDGP Project Members, 2000-, Butler, 2001) Dm0332 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation neb[TE12] 2L:20,076,518..20,076,518 reported_pr_change=S221L pr_change=S221L|neb-PA na_change=C20076518T (Ruden et al., 1997) point mutation neb[TE20] 2L:20,088,484..20,088,484 pr_change=Q760@|neb-PA reported_pr_change=Q760@ na_change=C20088484T (Ruden et al., 1997) point mutation neb[TE8] 2L:20,088,770..20,088,770 pr_change=Q855R|neb-PA reported_pr_change=Q855R na_change=A20088770G (Ruden et al., 1997) point mutation neb[TE2] 2L:20,089,246..20,089,246 pr_change=Q1014@|neb-PA reported_pr_change=Q1014@ na_change=C20089246T (Ruden et al., 1997)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 8176
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  oogenesis stage,adult stage  ovary (Alphey et al., 1997)  embryonic stage | mid  procephalic neuroblasts (Molina et al., 1997)  spermatogenesis,adult stage  testis (Alphey et al., 1997)  adult stage,spermatogenesis  spermatocyte (Molina et al., 1997)  embryonic stage,adult stage   (Molina et al., 1997)  adult stage,spermatogenesis  primary gonial cell (Molina et al., 1997)  adult stage   (Ohkura et al., 1997)  larval stage | third instar  imaginal disc (Alphey et al., 1997)  larval stage  larval brain | restricted (Molina et al., 1997)  embryonic stage   (Ohkura et al., 1997)  larval stage  imaginal disc | restricted (Molina et al., 1997)  embryonic stage | early   (Molina et al., 1997)  oogenesis stage,adult stage  nurse cell (Molina et al., 1997)  embryonic stage | mid  ventral nerve cord (Molina et al., 1997)  oogenesis stage,adult stage  follicle cell (Molina et al., 1997)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term	chromatin (Molina et al., 1997)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004374 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0004374 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004374
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable (with Df(2L)TW65) nebk02406 nebs4158 viable (with Df(2L)pr11163) nebk02406 viable (with Df(2L)pr9201) nebk02406 lethal nebCB04551 lethal | maternal effect | recessive nebk03903 lethal | recessive neb03552 neb1 nebMot nebk00802 nebk04805 nebk04912 nebk04914 nebk05702 nebk07614 nebs4158 lethal | recessive | rescuable maternal effect nebk06334 lethal | embryonic stage | maternal effect | recessive nebMot lethal | embryonic stage | recessive nebk00802 lethal | adult stage | recessive nebk00802 semi-lethal | larval stage | recessive neb01300 neb03552 neb05217 nebMot nebTE12 nebTE20 nebTE2 nebTE8 nebk02406 nebk05702 Other Phenotypes Allele wild-type nebHsp26.PA behavior defective | recessive neb01300 neb03552 neb05217 nebMot nebTE12 nebTE20 nebTE2 nebTE8 nebk02406 nebk05702 mitotic cell cycle defective neb01300 neb03552 neb05217 neb1 neb24-O neb93-E nebie1 nebk02406 nebk05702 mitotic cell cycle defective | germ-line clone nebTE12 nebTE20 nebTE8 Sterility Allele female sterile nebk00802 female sterile | recessive neb24-O female sterile | partially | recessive nebk06334 male sterile nebk00802 male sterile | recessive neb24-O nebk06334 Phenotype manifest in Allele macrochaeta & adult neb01300 neb03552 neb05217 nebTE12 nebTE2 nebTE20 nebTE8 nebk02406 nebk05702 interommatidial bristle neb01300 neb03552 neb05217 neb24-O nebTE12 nebTE2 nebTE20 nebTE8 nebk02406 nebk05702 wing neb01300 neb03552 neb05217 nebTE12 nebTE2 nebTE20 nebTE8 nebk02406 nebk05702 nuclear chromosome neb1 neb24-O neb93-E nebie1 centrosome neb1 neb24-O neb93-E nebie1 eye neb24-O nebk00802 nebk06334 ommatidium neb24-O nebk06334 adult abdominal sensillum neb24-O spermatozoon neb24-O egg chamber neb24-O ovary neb24-O follicle cell neb24-O adult neuroblast & mitosis & nuclear chromosome neb24-O adult neuroblast & spindle neb24-O macrochaeta nebL38BC nebk00802 nebk06334 embryonic abdomen | maternal effect nebMot macrochaeta & adult | maternal effect nebMot interommatidial bristle | maternal effect nebMot wing | maternal effect nebMot embryonic epidermis | germ-line clone nebTE12 nebTE2 nebTE20 nebTE8 embryonic/first instar larval cuticle | germ-line clone nebTE12 nebTE2 nebTE20 nebTE8 embryonic abdominal segment | germ-line clone nebTE12 nebTE2 nebTE20 nebTE8 embryonic head | germ-line clone nebTE12 nebTE2 nebTE20 nebTE8 external sensory organ nebk00802 multidendritic neuron nebk00802 chordotonal organ | lateral nebk00802 neuron nebk00802 egg | maternal effect nebk03903 embryo | maternal effect nebk03903 syncytial blastoderm embryo | maternal effect nebk03903 pole cell | maternal effect nebk03903 blastoderm embryo & nucleus | maternal effect nebk03903 spindle & embryo | maternal effect nebk03903 chromatin & embryo | maternal effect nebk03903 humeral bristle nebk06334 adult abdomen nebk06334 larval brain nebk06334 ovary & adult nebk06334 follicle cell & adult nebk06334
Classical Alleles ( 34 )
For All Classical Alleles Show
Allele of neb Class Mutagen Stocks Known lesion neb01300 loss of function P-element activity 0 Yes neb03552 loss of function P-element activity 1 Yes neb05217 loss of function P-element activity 0 Yes neb1 P-element activity 0 Yes neb24-O P-element activity 1 Yes neb93-E P-element activity 0 Yes nebBG02300 P-element activity 1 -- nebCB04551 P-element activity 0 Yes nebCB05794 P-element activity 0 Yes nebDG24610 1 -- nebEP642 P-element activity 1 -- nebEY11126 P-element activity 1 -- nebKG05913 P-element activity 1 Yes nebL38BC P-element activity 0 Yes nebMot loss of function ethyl methanesulfonate 0 Yes nebTE12 hypomorph ethyl methanesulfonate 0 Yes nebTE20 loss of function ethyl methanesulfonate 0 Yes nebTE2 loss of function ethyl methanesulfonate 0 Yes nebTE8 hypomorph ethyl methanesulfonate 0 Yes nebc00697 piggyBac transposase 1 -- nebc02666 piggyBac transposase 0 -- nebe00562 piggyBac transposase 1 -- nebie1 Δ2-3 0 Yes nebk00802 P-element activity 1 Yes nebk02406 loss of function P-element activity 0 Yes nebk03903 P-element activity 1 Yes nebk04805 P-element activity 0 Yes nebk04912 P-element activity 0 Yes nebk04914 P-element activity 0 Yes nebk05702 loss of function P-element activity 1 Yes nebk06334 hypomorph P-element activity 0 Yes nebk07614 P-element activity 0 -- nebk09314b P-element activity 0 Yes nebs4158 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of neb Class Mutagen Stocks Known lesion nebGD7072 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes nebHsp26.PA in vitro construct | regulatory fusion 0 Yes nebScer\UAS.T:Avic\GFP-EGFP in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes nebScer\UAS.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 1 Yes nebdsRNA.cGa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2L)TW150
Disrupted in	Df(2L)Sd14 (BDGP Project Members, 1994-1999) Df(2L)Sd57 (Butler et al., 2001) Df(2L)Sd77 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)TE38A-1 (BDGP Project Members, 1994-1999, Bhat et al., 1996) Df(2L)TW1 (Ohkura et al., 1997) Df(2L)TW12 (BDGP Project Members, 1994-1999) Df(2L)TW150 (Butler et al., 2001, BDGP Project Members, 1994-1999) Df(2L)TW161 (Spradling et al., 1999, BDGP