Gene Dmel\mei-41

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General Information

Symbol

Dmel\mei-41

Species

D. melanogaster

Name

meiotic 41

Annotation symbol

CG4252

Feature type

protein_coding_gene

FlyBase ID

FBgn0004367

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

1-54.2

Cytogenetic map

14C3-14C3

Sequence location

X:16,285,794..16,293,601 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene meiotic 41 is referred to in FlyBase by the symbol mei-41 (CG4252, FBgn0004367). It has the cytological map location 14C3. Its sequence location is X:16285794..16293601. Its molecular function is described as: protein kinase activity; kinase activity; binding; phosphotransferase activity, alcohol group as acceptor. It is involved in the biological processes described with 21 unique terms, many of which group under: cell cycle; cell cycle process; M phase of meiotic cell cycle; DNA repair; regulation of biological process; response to DNA damage stimulus; anatomical structure development; organelle organization and biogenesis; double-strand break repair via synthesis-dependent strand annealing; phosphorus metabolic process; response to radiation; gamete generation; embryonic development via the syncytial blastoderm; actin filament-based process. 63 alleles are reported. The phenotypes of these alleles are annotated with: egg; blastoderm embryo; cycle 13 embryo; cycle 14 embryo; spindle; eye disc; morphogenetic furrow; dorsal mesothoracic disc. It has one annotated transcript and one annotated polypeptide.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

mei-41 (R.S. Hawley)

mei-41 alleles confer sensitivity to mutagens as a consequence of a defect in a caffeine-sensitive post-replication repair pathway (Boyd and Setlow, 1976; Boyd et al., 1976; Boyd and Shaw, 1982). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker et al., 1978; Gatti, 1979). mei-411 not hypermutable to alkylation nor defective in excision repair; yet mei-41D12 displays hypermutability to alkylation and defective alkylation excision repair (Smith and Dusenberry, 1988, Mechanisms and Consequences of DNA Damage Processing, Alan R. Liss, Inc., pp. 251-55). Allelism based on lack of complementation (Mason, Scobie, and Yamamoto, 1989, Mol. Gen. Genet. 215: 190-99). Most alleles of mei-41 also reduce female fertility and in some cases are female-sterile as homozygotes. Females homozygous for the more fertile alleles of mei-41 exhibit reduced levels of meiotic exchange. The observed reductions in exchange are not uniform, but rather are most extreme in distal regions. Chiasma interference is also diminished (Carpenter and Sandler, 1974; Baker and Hall, 1976). These reduced levels of exchange allow for high frequencies of meiotic loss and nondisjunction (see Baker and Hall, 1976). Ultrastructural analysis of pachytene in mei-41 and mei-412 females demonstrates a reduced number of late recombination nodules which are distributed in a fashion that parallels residual exchange events (Carpenter, 1979). Over half of those nodules which are observed are morphologically abnormal and are associated with unusual regions of relatively uncondensed chromatin. Stocks in which mei-41 is carried in the male are frequently observed to carry or acquire a bb mutation on the mei-41-bearing X chromosome (Hawley and Tartof, 1983). Several alleles of mei-41 have also been shown to inhibit rDNA magnification in the male germline (Hawley and Tartof, 1983; Hawley et al., 1985). Moreover, mei-41 males undergoing magnification also produce a high frequency of X-Y exchanges which result from one break within the X-chromosome rDNA cluster and the other at any of a large number of sites on the Y chromosome (Hawley and Tartof, 1983).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

14C3-14C3

Limits computationally determined from genome sequence between P{EP}EP1493^EP1493 and P{EP}EP1547^EP1547

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

14C-14C

(determined by in situ hybridisation)

(Peter et al., 2002)

14C4-14C6

(determined by in situ hybridisation)

(Boyd et al., 1990)

14C4-14C6

(determined by in situ hybridisation)

(Yamamoto et al., 1990)

Experimentally Determined Recombination Data

Location

1-54.2

(Gatti, 1979)

(Smith, 1976, Mohler, 1977)

Left of (cM)

(Mohler, 1977)

Right of (cM)

mei-9

(Smith, 1976)

(Mohler, 1977)

Notes

Maps near 1-54.

(Smith, 1976)

mei-41^D14 maps approximately 0.05 units to the left of mei-41^D5.

(Mason et al., 1981)

mei-41^RT1 is located 0.04 map units to the right of mei-41^D14 and 0.02 map units to the left of mei-41^D9. mei-41^RT2 is located 0.06 map units to the right of mei-41^D14 and 0.03 map units to the right of mei-41^D9.

(Yamamoto et al., 1990)

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\mei-41 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

mei-41-RA

FBtr0074271

NM_078645

7554

2517

Additional Transcript Data & Comments

Reported size (kB)

8.3 (northern blot)

(Hari et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mei-41-PA

FBpp0074047

289.3

2517

7.58

NP_523369

AAF48604

Additional Polypeptide Data & Comments

Reported size (kD)

2354 (aa)

(Hari et al., 1995)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004367

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

HEAT (IPR000357)

Phosphatidylinositol 3- and 4-kinase, catalytic (IPR000403)

Immunoglobulin/major histocompatibility complex, conserved site (IPR003006)

PIK-related kinase, FAT (IPR003151)

PIK-related kinase, FATC (IPR003152)

Protein kinase-like (IPR011009)

UME (IPR012993)

PIK-related kinase (IPR014009)

Armadillo-type fold (IPR016024)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VXG8

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

complex substitution

mei-41[99B]
X:16,287,399..16,287,400

comment=The P{]mei-41[RT1] insertion is deleted in the mei-40[99B] strain leaving 40bp of mostly inverted repeat sequence inserted between the reported bases. This leads to a frameshift and early translation termination.
evidence=experimental

(Laurencon et al., 2003)

point mutation

mei-41[D13]-1
X:16,286,609..16,286,609

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). The reported nucleotide change predicts a W to R change rather than W to T as reported. Other amino acid changes common to several mei-41 mutants are also present in strain (see FBrf0160718).
evidence=experimental
na_change=T16286609A
pr_change=W253R|mei-41-PA
reported_na_change=T2585A
reported_pr_change=W90T

(Laurencon et al., 2003)

point mutation

mei-41[D3]
X:16,287,792..16,287,792

comment=Position of mutation on reference sequence inferred by FlyBase curator. Reported nucleotide change is not consistent with amino acid change.
evidence=experimental
na_change=C16287792T
pr_change=A647V|mei-41-PA
reported_na_change=T3768A
reported_pr_change=A384V

(Laurencon et al., 2003)

point mutation

mei-41[D9]
X:16,288,281..16,288,281

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
evidence=experimental
na_change=C16288281T
pr_change=S810F|mei-41-PA
reported_na_change=C4257T
reported_pr_change=S647F

(Laurencon et al., 2003)

point mutation

mei-41[D15]
X:16,288,352..16,288,352

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
evidence=experimental
na_change=A16288352C
pr_change=T815P|mei-41-PA
reported_na_change=A4328C
reported_pr_change=T652P

(Laurencon et al., 2003)

point mutation

mei-41[D14]
X:16,288,363..16,288,363

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
evidence=experimental
na_change=G16288363T
pr_change=L818F|mei-41-PA
reported_na_change=G4339T
reported_pr_change=L655F

(Laurencon et al., 2003)

point mutation

mei-41[D12]
X:16,292,077..16,292,077

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
evidence=experimental
na_change=C16292077T
pr_change=H2032Y|mei-41-PA
reported_na_change=C8053T
reported_pr_change=H1869Y

(Laurencon et al., 2003)

point mutation

mei-41[D5]
X:16,292,944..16,292,949

comment=Mutation lies in one of these two codons. There is a C to T change that causes one P to be an L however there is not enough informaton to determine which of the two P's is changed. The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718)
evidence=experimental
reported_na_change=C8924T
reported_pr_change=P2159L

(Laurencon et al., 2003)

point mutation

mei-41[D13]-2
X:16,292,948..16,292,948

comment=The mutation site reported in FBrf0160718 is relative to GB:U34925 (in which the predicted CDS is missing 163 N-terminal amino acids relative to genome release 3.2). Other amino acid changes common to several mei-41 mutants are also present in the strain (see FBrf0160718).
evidence=experimental
na_change=C16292948T
pr_change=P2322L|mei-41-PA
reported_na_change=C8924T
reported_pr_change=P2159L

(Laurencon et al., 2003)

rescue fragment

mei-41[+t10.5]
X:16,284,023..16,294,518

comment=The extent of the rescue fragment was deduced from FBrf0079877, FBrf0083174, and GB:U34925.
evidence=experimental

(Banga et al., 1995, Hari et al., 1995)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3065

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

oogenesis stage,adult stage | female

ovary

(Hari et al., 1995)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004367

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004367

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | rescuable maternal effect

lethal | embryonic stage | maternal effect | recessive

lethal | embryonic stage | recessive

mei-41^D5

Other Phenotypes

Allele

wild-type

mei-41^+t10.5

DNA repair defective

cell death increase

mei-41^29D

cell death decrease

mei-41^29D

meiotic cell cycle defective

mitotic cell cycle defective

mei-41^D3

mitotic cell cycle defective | recessive

chemical sensitive

chemical sensitive | recessive

chemical sensitive | dominant

mei-41^D3

chemical sensitive | semidominant

chemical sensitive | larval stage | recessive

mei-41^AI

radiation resistant

mei-41^D5

radiation sensitive

radiation sensitive | recessive

mei-41^D5

Sterility

Allele

female sterile

female sterile | conditional - heat sensitive

female sterile | partially

female sterile | partially | recessive

female semi-sterile

mei-41^29D

female semi-sterile | recessive

mei-41^13-1272

female fertile

female fertile | reduced

Phenotype manifest in

Allele

mitosis & nuclear chromosome

egg | maternal effect

eye disc | conditional qualifier

mei-41^29D

morphogenetic furrow | conditional qualifier

mei-41^29D

metaphase & condensed nuclear chromosome | conditional qualifier

mei-41^29D

dorsal mesothoracic disc

mei-41^29D

cycle 12 embryo & interphase of mitotic cell cycle | maternal effect

mei-41^D3

cycle 13 embryo & interphase of mitotic cell cycle | maternal effect

mei-41^D3

blastoderm embryo | maternal effect

mei-41^D3

cycle 13 embryo

mei-41^D3

cycle 14 embryo

mei-41^D3

spindle

mei-41^D3

neuroblast & third instar larva

mei-41^D3

Classical Alleles ( 61 )

For All Classical Alleles Show

Allele of mei-41	Mutagen	Stocks	Known lesion
mei-41^11-1325	ethyl methanesulfonate	0	--
mei-41^11-98	ethyl methanesulfonate	0	--
mei-41^12-1483	ethyl methanesulfonate	0	--
mei-41^12-3616	ethyl methanesulfonate	0	--
mei-41^12C-20	ethyl methanesulfonate	0	--
mei-41^13-1272	ethyl methanesulfonate	0	--
mei-41¹		0	--
mei-41^29D	P-element activity	0	Yes
mei-41²		2	--
mei-41^99B	P-element activity	0	Yes
mei-41^A10	ethyl methanesulfonate	0	--
mei-41^A11	ethyl methanesulfonate	0	--
mei-41^A12	ethyl methanesulfonate	0	--
mei-41^A13	ethyl methanesulfonate	0	--
mei-41^A14	ethyl methanesulfonate	0	--
mei-41^A15	ethyl methanesulfonate	0	--
mei-41^A16	ethyl methanesulfonate	0	--
mei-41^A17	ethyl methanesulfonate	0	--
mei-41^A1		0	--
mei-41^A22		0	--
mei-41^A27		0	--
mei-41^A28		0	--
mei-41^A29		0	--
mei-41^A2	ethyl methanesulfonate	0	--
mei-41^A3	ethyl methanesulfonate	0	--
mei-41^A4	ethyl methanesulfonate	0	--
mei-41^A5	ethyl methanesulfonate	0	--
mei-41^A6	ethyl methanesulfonate	0	--
mei-41^A7	ethyl methanesulfonate	0	--
mei-41^A8	ethyl methanesulfonate	0	--
mei-41^A9	ethyl methanesulfonate	0	--
mei-41^AI		0	--
mei-41^D10	ethyl methanesulfonate	0	--
mei-41^D11	ethyl methanesulfonate	0	--
mei-41^D12		1	--
mei-41^D13		0	--
mei-41^D14		4	--
mei-41^D15	ethyl methanesulfonate	0	--
mei-41^D16		0	--
mei-41^D18	ethyl methanesulfonate	0	--
mei-41^D19		0	--
mei-41^D1		1	--
mei-41^D20	ethyl methanesulfonate	0	--
mei-41^D21	ethyl methanesulfonate	0	--
mei-41^D22	ethyl methanesulfonate	0	--
mei-41^D2		1	--
mei-41^D3		1	Yes
mei-41^D4		0	--
mei-41^D5		30	--
mei-41^D6	ethyl methanesulfonate	0	--
mei-41^D7	ethyl methanesulfonate	0	--
mei-41^D8	ethyl methanesulfonate	0	--
mei-41^D9	ethyl methanesulfonate	1	--
mei-41^G0221b	P-element activity	1	--
mei-41^RT1	PM hybrid dysgenesis P-element activity	2	Yes
mei-41^RT1R1	P-element activity	0	Yes
mei-41^RT2	PM hybrid dysgenesis P-element activity	1	Yes
mei-41^RT2R1	P-element activity	0	Yes
mei-41^RT2R2	P-element activity	0	Yes
mei-41^e02381	piggyBac transposase	2	--
mei-41^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mei-41	Class	Mutagen	Stocks	Known lesion
mei-41^+t10.5		in vitro construct \| genomic fragment	0	Yes
mei-41^GD4258		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)19

Df(1)l32

Disrupted in

Df(1)78B

(Laurencon et al., 2003)

Df(1)l32

(Banga et al., 1995)

Duplicated in

Dp(1;2)r⁺75c

(Mason et al., 1981, Eeken et al., 1985, Banga et al., 1995)

Dp(1;2)v⁺75d

(Mason et al., 1981)

Dp(1;4)81l8g

(Banga et al., 1995)

Dp(1;4)r⁺l

(Mason et al., 1981, Eeken et al., 1985)

Not disrupted in

Df(1)1D1

(Mason et al., 1981)

Df(1)C246

(Mason et al., 1981)

Df(1)C52

(Mason et al., 1981)

Df(1)HA85

(Mason et al., 1981)

Df(1)HA92

(Mason et al., 1981)

Df(1)HF349

(Mason et al., 1981)

Df(1)JA26

(Mason et al., 1981)

Df(1)KA6

(Mason et al., 1981)

Df(1)KA9

(Mason et al., 1981)

Df(1)N71

(Mason et al., 1981)

Df(1)RA37

(Mason et al., 1981)

Df(1)g

(Mason et al., 1981)

Df(1)r-D1

(Eeken et al., 1985, Banga et al., 1995)

Df(1)sd72b

(Mason et al., 1981)

Df(1)v-L15

(Mason et al., 1981)

Not duplicated in

Dp(1;3)f⁺71b

(Mason et al., 1981, Eeken et al., 1985)

Dp(1;4)80f18c

(Banga et al., 1995)

Dp(1;4)81h12b

(Banga et al., 1995)

Dp(1;4)81l12h

(Banga et al., 1995)

Dp(1;4)82c3k

(Banga et al., 1995)

Dp(1;4)exd^81h24b

(Banga et al., 1995)

Dp(1;Y)v⁺y⁺

(Mason et al., 1981)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD4258}

characterization construct

P{mei-41^+t10.5}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}mei-41^e02381

P{5'3'}mei-41^99B

P{}mei-41^RT1

P{}mei-41^RT2

insertion of enhancer trap

P{lacW}mei-41^G0221b

Related Comments

Please look at the allele reports for the complete phenotype data

mei-41 mutants show defects in repair of double-strand breaks; they are defective in completing the later steps of homologous recombination repair, but show no defects in end-joining repair.

(LaRocque et al., 2007)

mei-41 alleles confer sensitivity to mutagens as a consequence of a defect in a caffeine-sensitive post-replication repair pathway (Boyd and Setlow, 1976; Boyd, Golino, Nguyen and Green, 1976; Boyd and Shaw, 1982). This defect in DNA repair is also manifested by a high frequency of mitotic chromosome breakage and instability (Baker, Carpenter and Ripoll, 1978; Gatti, 1979). mei-41¹ not hypermutable to alkylation nor defective in excision repair; yet mei-41^D12 displays hypermutability to alkylation and defective alkylation excision repair (Smith and Dusenberry, 1988). Allelism based on lack of complementation (Mason et al., 1989). Most alleles of mei-41 also reduce female fertility and in some cases are female-sterile as homozygotes. Females homozygous for the more fertile alleles of mei-41 exhibit reduced levels of meiotic exchange. The observed reductions in exchange are not uniform, but rather are most extreme in distal regions. Chiasma interference is also diminished (Carpenter and Sandler, 1974; Baker and Hall, 1976). These reduced levels of exchange allow for high frequencies of meiotic loss and nondisjunction (see Baker and Hall, 1976). Ultrastructural analysis of pachytene in mei-41 and mei-41² females demonstrates a reduced number of late recombination nodules which are distributed in a fashion that parallels residual exchange events (Carpenter, 1979). Over half of those nodules which are observed are morphologically abnormal and are associated with unusual regions of relatively uncondensed chromatin. Stocks in which mei-41 is carried in the male are frequently observed to carry or acquire a bb mutation on the mei-41-bearing X chromosome (Hawley and Tartof, 1983). Several alleles of mei-41 have also been shown to inhibit rDNA magnification in the male germ-line (Hawley and Tartof, 1983; Hawley et al., 1985). Moreover, mei-41 males undergoing magnification also produce a high frequency of X-Y exchanges which result from one break within the X-chromosome rDNA cluster and the other at any of a large number of sites on the Y chromosome (Hawley and Tartof, 1983).