Gene Dmel\Hrb87F

87F-87F

(determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: sqd- Hrb87F- B52+

Gene Order (overall orientation not stated)

References

Overall orientation not stated: sqd- rin+ Rbp4- Hrb87F- B52+

(Haynes et al., 1997)

Gene Model & Products

Please see the GBrowse view of Dmel\Hrb87F for information on other features

Comments on Gene Model

DGC clone GH05625 appears problematic: incomplete CDS

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Hrb87F-RA

FBtr0082851

NM_057459

2270

385

Hrb87F-RB

FBtr0082852

NM_057458

2090

325

Additional Transcript Data & Comments

Reported size (kB)

2.2, 1.7 (northern blot)

1.1 (longest cDNA)

2.3, 1.8 (compiled cDNA)

(Hovemann et al., 1991, Hovemann et al., 1991)

Comments

The 2.3kb and 1.8kb Hrb87F transcripts differ only in the site of polyadenylation.

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Hrb87F-PA

FBpp0082316

39.5

385

9.46

NP_476807

AAF54967

Hrb87F-PB

FBpp0082317

33.7

325

9.19

NP_476806

AAN13574

Additional Polypeptide Data & Comments

Reported size (kD)

326 (aa); 34 (kD observed); 34 (kD predicted)

386 (aa); 38 (kD observed); 40 (kD predicted)

36 (kD observed)

386 (aa); 40 (kD predicted)

Comments

Staining of polytene chromosomes with antibody against Hrb87F protein reveals a unique banding pattern.

One of a couple of protein products.

(FlyBase, 1992-, FlyBase, 1992-)

hnRNP-specific mAbs were obtained and used to purify hnRNP complexes from S2 cells. hnRNP proteins were identified on the basis of nucleoplasmic localization, association with nascent transcripts, crosslinking to poly(A)-containing RNA in living cells, and amino acid sequence. Staining of polytene chromosomes reveals that Hrb87F protein is associated with actively transcribing loci.

Hrb87F protein has two amino-terminal RNP-CS RNA-binding domains and a carboxy-terminal glycine-rich auxiliary domain.

(BDGP Project Members, 2000-, Oliver, 2001.8.16)

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0004237

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RNA recognition motif, RNP-1 (IPR000504)

Nucleotide-binding, alpha-beta plait (IPR012677)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P48810

UniProtKB/TrEMBL

Maps to

(Deak et al., 1997)

FBcl0298053

(FlyBase, 1992-)

Does NOT map to

(Deak et al., 1997)

Identified with

GH05625

LD32727

(BDGP Project Members, 2000-)

bs14e11.y1

(Oliver, 2001.8.16)

D1178

(BDGP Project Members, 1994-1999)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9349

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

oogenesis stage,adult stage | female

ovary

embryonic stage-adult stage

oogenesis stage,adult stage | female

ovary

embryonic stage-adult stage

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

nucleus

nucleus chromatin

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI107775

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0004237

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0004237

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0004237

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Phenotype manifest in

Allele

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Hrb87F	Class	Mutagen	Stocks	Known lesion
Hrb87F²⁸		Δ2-3	0	Yes
Hrb87F⁷		Δ2-3	0	Yes
Hrb87F^CC00189		P-element activity	0	Yes
Hrb87F^KG02089		P-element activity	1	--
Hrb87F^Y1217	wild-type allele	P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Hrb87F	Mutagen	Stocks	Known lesion
Hrb87F^GD7818	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
Hrb87F^{hs.T:Zzzz\FLAG}	in vitro construct \| coding region fusion in vitro construct \| regulatory fusion	0	Yes
Hrb87F^tHa	in vitro construct \| genomic fragment	0	Yes
Hrb87F^tHb	in vitro construct \| genomic fragment	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)urd

Disrupted in

Df(3R)B52²⁸

Df(3R)B52^R1

Df(3R)Exel6169

(Ryder, 2004.4.26)

Df(3R)Hrb87F

(Zu et al., 1996, Sengupta, 2006)

Df(3R)Hrb87F⁷

Df(3R)l26c

Df(3R)urd

Not disrupted in

Df(3R)red3l

Df(3R)ry27

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD7818}

characterization construct

P{Hrb87F.Hb}

P{Hrb87F}

heat-shock construct

P{FLAG/HRB87F}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{PTT-GC}Hrb87F^CC00189

P{SUPor-P}Hrb87F^KG02089

insertion of enhancer trap

P{lacW}Hrb87F^Y1217

Related Comments

Please look at the allele reports for the complete phenotype data

Homology to the mammalian A and B hnRNP proteins based on amino acid sequence inferred from nucleotide sequence.

Hrb87F has been cloned and sequenced.

Hrb87F has been cloned and sequenced.

An Hrb87F cDNA clone has been isolated and sequenced.