A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\γTub23C

General Information
SymbolDmel\γTub23CSpeciesD. melanogaster
Nameγ-Tubulin at 23CAnnotation symbolCG3157
Feature typeprotein_coding_geneFlyBase IDFBgn0004176
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2LRecombination map
Cytogenetic map23C4-23C4Sequence location2L:2,972,892..2,974,862 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene γ-Tubulin at 23C is referred to in FlyBase by the symbol γTub23C (CG3157, FBgn0004176). It has the cytological map location 23C4. Its sequence location is 2L:2972892..2974862. Its molecular function is described as: structural constituent of cytoskeleton; GTP binding; microtubule minus-end binding; guanyl nucleotide binding; GTPase activity. It is involved in the biological processes: microtubule-based process; microtubule nucleation; regulation of cell cycle; centrosome organization and biogenesis; mitotic spindle organization and biogenesis; protein polymerization. 15 alleles are reported. The phenotypes of these alleles are annotated with: larval brain; imaginal disc; spermatid; eye; wing; wing blade; wing vein; spindle microtubule; spindle. It has one annotated transcript and one annotated polypeptide.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
23C4-23C4  
Limits computationally determined from genome sequence between P{PZ}lilli00632 and P{PZ}toc01361  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
23C1-23C4
(determined by in situ hybridisation)  
(Sunkel et al., 1995)
23C-23D
(determined by in situ hybridisation)  
(Zheng et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
(Huang et al., 1998)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: Rbp9+ Ts? Rrp1- γTub23C+ anon-23Cb? anon-23Cc? okr?
(Ghabrial et al., 1998)
Gene Order (overall orientation not stated)
References
Overall orientation not stated: γTub23C- Rrp1+ Ts+ Rbp9-
(Tsoi, 1998.6.19)
hide Gene Model & Products
Please see the GBrowse view of Dmel\γTub23C for information on other features
detailed view FBtr0290241 FBtr0077678 FBtr0077641 FBtr0077677 FBtr0077676 FBpp0288680 FBpp0077362 FBpp0077326 FBpp0077361 FBpp0077360 FBti0051118 FBti0021666 FBti0007753 FBti0003570 FBti0025992 FBti0071036
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
γTub23C-RA
FBtr0077641
 NM_057456
  1853
  475
Additional Transcript Data & Comments
Reported size (kB)
1.875 (longest cDNA)
(Zheng et al., 1991)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
gammaTub23C-PA  
FBpp0077326  
53.3  
475  
5.78  
  NP_476804
  AAF51174
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Tubulin (IPR000217)
Gamma tubulin (IPR002454)
Tubulin/FtsZ, GTPase (IPR003008)
Tubulin/FtsZ, C-terminal (IPR008280)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AC010009
 
AC092187
 
AE003581
AAF51174
 
AF073994
AAC27620
 
AI136006
 
AI406263
 
AI514354
 
AI531067
 
AW943499
 
AY069633
AAL39778
 
BF485702
 
BF485718
 
BF485719
 
BF485743
 
BF485744
 
BF487180
 
BF495310
 
BI354266
 
BI637398
 
BI640028
 
BP547240
 
CO152833
 
CO181861
 
CO194465
 
CO278028
 
CO309403
 
CO325068
 
CO327885
 
CO330384
 
CO331621
 
CO332236
 
EC074882
 
EC077676
 
EC214847
 
EC228082
 
EC233123
 
EC239723
 
EC247819
 
EC263579
 
M61765
AAA28597
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    (FlyBase, 1992-)
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    gammaub23C[bmps1]
    2L:2,974,322..2,974,322
    comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
    evidence=experimental
    na_change=G2974322H
    pr_change=M382I|gammaTub23C-PA
    reported_pr_change=M382I
    (Parks, 2004.5.6)
    rescue fragment
    gammaub23C[+t9]
    2L:2,970,422..2,979,757
    comment=Approximate boundaries of genomic fragment which rescues $ggr;Tub23C[PI]; exact endpoints not reported.
    evidence=experimental
    linked_to=EcoRI-HindIII_rfrag
    (Sunkel et al., 1995)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0290241 FBtr0077678 FBtr0077641 FBtr0077677 FBtr0077676
        AI514354
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for γTub23C
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        lethal
        lethal | larval stage | recessive
        Other Phenotypes
        Allele
        wild-type
        visible | dominant
        visible | dominant | conditional - heat sensitive
        mitotic cell cycle defective
        mitotic cell cycle defective | recessive
        Phenotype manifest in
        Allele
        eye
        wing
        wing blade
        wing vein
        larval brain
        imaginal disc
        spermatocyte & spindle
        spermatocyte & aster
        spermatid
        sensillum campaniformium of dorsal radius & pupa
        wing | conditional - heat sensitive
        spindle microtubule
        spindle
        hide Classical Alleles ( 8 )
        For All Classical Alleles Show

        Allele of γTub23CClassMutagenStocksKnown lesion
        γTub23C2
          		0 --
          γTub23CPIloss of function0 Yes
          γTub23Cbmps11 Yes
          γTub23Cbmps20 Yes
          γTub23Cbmps30 Yes
          γTub23Cbmps40 Yes
          γTub23Cbmps50 Yes
          γTub23Cunspecified
            		0 --
            hide Alleles Carried on Transgenic Constructs ( 7 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of γTub23CClassMutagenStocksKnown lesion
            γTub23C+t90 Yes
            γTub23C+tLa0 Yes
            γTub23CAct5C.T:Avic\GFP0 Yes
            γTub23CGD163971 Yes
            γTub23CGD87491 Yes
            γTub23CdsRNA.cMa0 --
            γTub23CΔ0 Yes
            hide Aneuploid Aberrations
            Useful deficiency
             
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            characterization construct
            P{w-γ-23C}
            NA
            P{w-γ-23CΔ}
            NA
            P{γTub23C+tLa}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            miscellaneous insertions
            P{hsneo}γTub23CPI
            NA
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            A γTub23C cDNA has been cloned and sequenced.
            (Zheng and Oakley, 1989)
            γTub23C has been cloned and sequenced.
            (Zheng et al., 1991)
            Drosophila γ tubulin is part of a complex containing Cp190 and Map60 gene products.
            (Raff et al., 1993)
            Subcellular localisation of the protein during the cell cycle of cells with and without centrioles is determined. Data suggests centrioles are not essential to the formation of a functional mitotic spindle, but participate in the focal concentration of γTub23C and γTub37C protein during both interphase and mitosis.
            (Debec et al., 1995)
            γTub23C is required for the structure as well as the function of the microtubule organising center MTOC.
            (Sunkel et al., 1995)
            γTub23C is required for spermatogenesis.
            (Sampaio and Sunkel, 1995)
            Immunoblot analysis and immunolocalisation show that γTub23C and γTub37C are differentially expressed during gametogenesis and development.
            (Wilson et al., 1997)
            SL2 cells treated with dsRNA against γTub23C show a clear increase in the mitotic index compared to control cells. The treated cells show bipolar monastral and monopolar anastral spindle organisation with metaphase-like chromosome arrangements.
            (Muller et al., 2006)
            RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of an aberrantly long, monopolar spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
            (Goshima et al., 2007)
            hide Gene Ontology: Function, Process & Cellular Component ( 15 )
            hide Molecular Function
            CV term
            Evidence
            References
            GTP binding
            inferred from direct assay
            (Gunawardane et al., 2003)
            GTPase activity
            inferred from electronic annotation with InterPro:IPR008280
            (FlyBase Curators et al., 2004-)
            guanyl nucleotide binding
            inferred from direct assay
            (Gunawardane et al., 2000)
            contributes_to
            microtubule minus-end binding
            inferred from direct assay
            (Gunawardane et al., 2000)
            structural constituent of cytoskeleton
            inferred from sequence or structural similarity with UniProtKB:P23330
            (FlyBase, 1992-)
            inferred from sequence or structural similarity
            (Goldstein and Gunawardena, 2000)
            inferred from sequence or structural similarity
            (Goldstein and Gunawardena, 2000)
            hide Biological Process
            CV term
            Evidence
            References
            centrosome organization and biogenesis
            inferred from mutant phenotype
            (Goshima et al., 2007)
            microtubule nucleation
            inferred from mutant phenotype
            (Mahoney et al., 2006)
            inferred from mutant phenotype
            (M Mahoney, 2006)
            inferred from direct assay
            (Gunawardane et al., 2000)
            microtubule-based process
            inferred from sequence or structural similarity
            (Goldstein and Gunawardena, 2000)
            non-traceable author statement
            (Swiss-Prot Project Members, 1991.11.1)
            mitotic spindle organization and biogenesis
            inferred from mutant phenotype
            (Goshima et al., 2007)
            protein polymerization
            inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280
            (FlyBase Curators et al., 2004-)
            regulation of cell cycle
            inferred from mutant phenotype
            (Muller et al., 2006)
            hide Cellular Component
            CV term
            Evidence
            References
            gamma-tubulin small complex
            inferred from sequence or structural similarity with UniProtKB:P23330
            (FlyBase, 1992-)
            inferred from physical interaction with FLYBASE:Grip84; FB:FBgn0026430 inferred from physical interaction with FLYBASE:l(1)dd4; FB:FBgn0001612
            (Gunawardane et al., 2000)
            microtubule
            inferred from sequence or structural similarity with UniProtKB:P23330
            (FlyBase, 1992-)
            inferred from sequence or structural similarity
            (Goldstein and Gunawardena, 2000)
            inferred from sequence or structural similarity
            (Goldstein and Gunawardena, 2000)
            microtubule organizing center
            inferred from sequence or structural similarity with UniProtKB:P23330
            (FlyBase, 1992-)
            non-traceable author statement
            (Swiss-Prot Project Members, 1991.11.1)
            tubulin complex
            non-traceable author statement
            (Swiss-Prot Project Members, 1991.11.1)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            γTub23C allele
            Gene
            References
            γTub23CPI
            γTub37C
            (Tavosanis and Gonzalez, 2003)
            unspecified
            N
            (Mahoney et al., 2006, Vazquez et al., 2006)
            brm
            (Vazquez et al., 2006)
            Psn
            (Mahoney et al., 2006)
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 3 )
            Bloomington
            8399
            γTub23Cbmps1/CyO
            VDRC
            v19130
            w1118; P{GD8749}v19130
            v47163
            w1118 P{GD16397}v47163
            hide Genomic Clones ( 1 )
            hide cDNA Clones ( 24 )
            Please Note
            This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Additional comments
            hide Comments About Role
            hide Comments About Molecular Function
            hide Other Comments
            Isolated from a D.melanogaster ovary cDNA library using an A.nidulans mipA gene cDNA probe, under low stringency conditions.
            (Zheng and Oakley, 1989)
            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
            (Kiger et al., 2003)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •