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Gene Dmel\5-HT1A

General Information
Symbol	Dmel\5-HT1A	Species	D. melanogaster
Name	Serotonin receptor 1A	Annotation symbol	CG16720
Feature type	protein_coding_gene	FlyBase ID	FBgn0004168
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	56B2-56B5	Sequence location	2R:14,954,664..15,009,463 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Serotonin receptor 1A is referred to in FlyBase by the symbol 5-HT1A (CG16720, FBgn0004168). It has the cytological map location 56B2-56B5. Its sequence location is 2R:14954664..15009463. Its molecular function is described as: serotonin receptor activity; 5-HT1 receptor activity; G-protein coupled serotonin receptor activity; amine receptor activity. It is involved in the biological processes: serotonin receptor, adenylate cyclase inhibiting pathway; serotonin receptor, phospholipase C activating pathway; serotonin receptor signaling pathway; regulation of circadian sleep/wake cycle, sleep. 6 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 56B2-56B5   Limits computationally determined from genome sequence between P{lacW}prodk08810 and P{PZ}ena02029&P{lacW}corak08713
Experimentally Determined Cytological Location
Cytogenetic map Notes References 56A-56B (determined by in situ hybridisation)   (Saudou et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\5-HT1A for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) 5-HT1A-RA FBtr0086578  NM_057454   4226   834 5-HT1A-RB FBtr0086577  NM_166322   5015   834
Additional Transcript Data & Comments
Reported size (kB)	6.2 (northern blot) (Saudou et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein 5-HT1A-PA   FBpp0085762   89.6   834   5.65     NP_476802   AAF57603 5-HT1A-PB   FBpp0085761   89.6   834   5.65     NP_725849   AAM68432
Additional Polypeptide Data & Comments
Reported size (kD)	834 (aa) (Saudou et al., 1992)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0004168
Crossreferences	GPCRDB - Information system for G protein-coupled receptors • 0254 InterPro domains - A database of protein families, domains, and functional sites • GPCR, rhodopsin-like (IPR000276) GPCR, rhodopsin-like superfamily (IPR017452)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC007839   AE003797 AAF57603   AE003797 AAM68432   AI259194   BI369389   BI370929   BT023487   CG16720   DD177548   Z11489 CAA77570
UniProtKB/Swiss-Prot	P28285
UniProtKB/TrEMBL
Maps to	DS07626 (Hita et al., 1999) FBcl0297732 (FlyBase, 1992-)
Does NOT map to
Identified with	RE57708 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 11885
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 16,17  midline (Saudou et al., 1992)  embryonic stage | stage 16,17  embryonic central nervous system (Saudou et al., 1992)  embryonic stage | stage 16-17  VUM neuron (Saudou et al., 1992)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004168 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004168
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele behavior defective 5-HT1AΔ5kb Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of 5-HT1A Class Mutagen Stocks Known lesion 5-HT1AEY09988 P-element activity 1 Yes 5-HT1AΔ5kb P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of 5-HT1A Class Mutagen Stocks Known lesion 5-HT1AGD618 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes 5-HT1AScer\UAS.T:SV5\V5 in vitro construct | coding region fusion 0 Yes 5-HT1AScer\UAS.cRa in vitro construct | regulatory fusion 0 -- 5-HT1AΔ5kb.Scer\UAS.T:SV5\V5 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes
Aneuploid Aberrations
Useful duplication	Dp(2;Y)53D;57C
Disrupted in	Df(2R)Exel6068 (Ryder, 2004.4.26) Df(2R)Δ5kb (Yuan et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD618} NA P{UAS-5-HT1A-V5} NA P{UAS-5-HT1A.R} NA P{UAS-5-HT1A.Δ-V5} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}5-HT1AEY09988 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
5-HT1 receptor activity non-traceable author statement (Tierney, 2001)
G-protein coupled serotonin receptor activity non-traceable author statement (Swiss-Prot Project Members, 1992.12.1) inferred from sequence or structural similarity (Brody and Cravchik, 2000)
amine receptor activity inferred from sequence or structural similarity (Radford et al., 2002)
serotonin receptor activity inferred from sequence or structural similarity with MGD:Htr1a; MGI:MGI:96273 (FlyBase, 1992-) traceable author statement (Tierney, 2001)
Biological Process
CV term Evidence References
regulation of circadian sleep/wake cycle, sleep inferred from mutant phenotype (Yuan et al., 2006)
serotonin receptor signaling pathway inferred by curator from GO:0004993 (FlyBase, 1992-) inferred from sequence or structural similarity (Brody and Cravchik, 2000)
serotonin receptor, adenylate cyclase inhibiting pathway inferred from direct assay (Saudou et al., 1992) traceable author statement (Tierney, 2001) non-traceable author statement (Swiss-Prot Project Members, 1992.12.1)
serotonin receptor, phospholipase C activating pathway inferred from direct assay (Saudou et al., 1992) traceable author statement (Tierney, 2001)
Cellular Component
CV term Evidence References
integral to membrane inferred from sequence or structural similarity with MGD:Htr1a; MGI:MGI:96273 (FlyBase, 1992-) inferred from sequence or structural similarity (Brody and Cravchik, 2000)
integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1992.12.1)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions 5-HT1A allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62861
Orthologs
Genome-wide drosophilid orthologs	Dere\GG21944 Dgri\GH22141 Dmoj\GI19372 Dper\GL11505 Dpse\GA14104 Dsim\GD11428 Dvir\GJ19928 Dwil\GK20939 Dyak\GE12018
Curated drosophilid orthologs	Dpse\GA14104   Dsim\5-HT1A (Nikoh et al., 2004)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000018839 Anopheles gambiae (Ensembl) ENSCINP00000009890 Ciona intestinalis (Ensembl) NEWSINFRUP00000154633 Takifugu rubripes (Ensembl) ENSMODP00000021137 Monodelphis domestica (Ensembl) ENSRNOP00000013618 Rattus norvegicus (Ensembl) GSTENP00006603001 Tetraodon nigroviridis (Ensembl) GSTENP00009075001 Tetraodon nigroviridis (Ensembl) WBGene00035445 Caenorhabditis briggsae (Wormbase) ZDB-GENE-030616-574 Danio rerio (Zfin)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
Bloomington	17629 y1 w67c23; P{EPgy2}5-HT1AEY09988
Genomic Clones ( 1 )
	BACR01N09
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE57708
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE55558 LP02550
Other clones
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004168 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004168
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: 5-HT1A CG16720 (Lai, 1999.11)
Source for database merge of
Additional comments
Comments About Role
	One of several known Drosophila serotonin receptor encoding genes. Presumed to be a recent duplication of 5-HT1B gene.   5-HT1A receptor inhibits adenylate cyclase and activates phospholipase C. Expression starts in late embryos predominantly in midline motor neurones, suggesting a role in motor control. 5-HT1A and 5-HT1B have a common chromosomal location and high sequence homology suggesting they are the result of a recent duplication event. (Saudou et al., 1992)
Comments About Molecular Function
	Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. (Claridge-Chang et al., 2001)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057454 NM_166322 RefSeq (Proteins) • NP_476802 NP_725849 DDBJ / EMBL / GenBank • AC007839 AE003797 AAF57603 AAM68432 BI370929 DD177548 Z11489 CAA77570 UniProtKB/Swiss-Prot • P28285
Other Crossreferences
	GPCRDB - Information system for G protein-coupled receptors • 0254 InterPro domains - A database of protein families, domains, and functional sites • GPCR, rhodopsin-like (IPR000276) GPCR, rhodopsin-like superfamily (IPR017452)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62861 DEDB - Drosophila exon database: splicing graphs • 11885 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0004168 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0004168 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0004168 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0004168 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0004168 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000018839 Anopheles gambiae (Ensembl) ENSCINP00000009890 Ciona intestinalis (Ensembl) NEWSINFRUP00000154633 Takifugu rubripes (Ensembl) ENSMODP00000021137 Monodelphis domestica (Ensembl) ENSRNOP00000013618 Rattus norvegicus (Ensembl) GSTENP00006603001 Tetraodon nigroviridis (Ensembl) GSTENP00009075001 Tetraodon nigroviridis (Ensembl) WBGene00035445 Caenorhabditis briggsae (Wormbase) ZDB-GENE-030616-574 Danio rerio (Zfin) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/serotr1aandb1.htm PANTHER - Protein classification by function, families, and pathways • FBgn0004168
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	5HT1A (Witz et al., 1991) 5-HT1A (Wheeler et al., 2006, Riedl et al., 2007) 5-HT1ADro (Maroteaux, 1995.5.31) 5HT1ADro (Kerr et al., 2004) 5-HT1ADro (Tierney, 2001) 5HT-dro2A (Tierney, 2001, Boschert et al., 1991, Boschert et al., 1991, Buchner, 1991, ) 5-HT-dro2A (Tierney, 2001) 5htdro2a (Feng et al., 1996) 5HT-drp2A (Hen, 1993) 5HT-R2A   CG16720 (Bryson-Richardson et al., 2004, ) d5-HT1A (Yuan et al., 2006) Dm5HTdro2A (Blenau and Baumann, 2001) DRO2A (Han et al., 1998)
Name Synonym	Serotonin receptor 1A   serotonin-receptor-2A (Boschert et al., 1991) Serotonin receptor 2A
Secondary FlyBase IDs
References ( 36 )
Generate a list of	All references relating to this gene ( 36 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 1 ) Review  ( 4 ) Personal communication to FlyBase  ( 2 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Letter  ( 1 ) Automatic genome annotation  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 3 )
	Riedl et al., 2007, Fly 1(1): 23--32 Genetic and behavioral analysis of natural variation in Drosophila melanogaster pupation position. [FBrf0200063] Wheeler et al., 2006, Dev. Biol. 294(2): 509--524 Single-cell mapping of neural and glial gene expression in the developing Drosophila CNS midline cells. [FBrf0195284] Yuan et al., 2006, Curr. Biol. 16(11): 1051--1062 A sleep-promoting role for the Drosophila serotonin receptor 1A. [FBrf0195242] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2006 Show reviews ...