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A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008

Gene Dmel\Pp1-87B

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General Information

Symbol

Dmel\Pp1-87B

Species

D. melanogaster

Name

Protein phosphatase 1 at 87B

Annotation symbol

CG5650

Feature type

protein_coding_gene

FlyBase ID

FBgn0004103

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

3R

Recombination map

Cytogenetic map

87B9-87B10

Sequence location

3R:8,249,335..8,251,639 [-]

Map ( GBrowse )

detailed view

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Summary Information

Automatically generated summary

See sections below for more information

The gene Protein phosphatase 1 at 87B is referred to in FlyBase by the symbol Pp1-87B (CG5650, FBgn0004103). It has the cytological map location 87B9-87B10. Its sequence location is 3R:8249335..8251639. Its molecular function is described as: protein serine/threonine phosphatase activity; myosin phosphatase activity. It is involved in the biological processes described with 14 unique terms, many of which group under: cell cycle; learning and/or memory; cell cycle process; organelle organization and biogenesis; anatomical structure development; learning; behavior; chromosome organization and biogenesis; transport; biopolymer modification; locomotion; gamete generation; olfactory learning; locomotory behavior. 19 alleles are reported. The phenotypes of these alleles are annotated with: nuclear chromosome; larval optic neuropil; neuroblast; ganglion mother cell; imaginal disc. It has one annotated transcript and one annotated polypeptide.

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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

l(3)87Bg

Hemizygotes for l(3)87Bg2 form fully-pigmented pharate adults. Viability reduced in certain trans heterozygotes with Su(var)3-61; several l(3)87Bg/+ genotypes suppress position-effect variegation (Reuter et al.).

Pp1: Protein phosphatase 1

Encodes a protein phosphatase 1 catalytic subunit that shows a high degree of similarity to rabbit protein phosphatase 1α in its enzymatic and physiochemical characteristics and has a predicted protein sequence that is 92% identical to that of rabbit PP-1α.

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B9-87B10

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87B10-87B13

(determined by in situ hybridisation)

(Spradling et al., 1999)

87B6-87B12

(determined by in situ hybridisation)

(Ranz et al., 1997)

87B10-87B13

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

87B6-87B12

(determined by in situ hybridisation)

(Axton et al., 1990)

87B4-87B7

Location based on deficiency mapping.

(Wustmann et al., 1989)

87B6-87B12

(determined by in situ hybridisation)

(Dombradi et al., 1989)

Experimentally Determined Recombination Data

Location

3-51.1 +/- 0.5

(Reuter et al., 1986, Wustmann et al., 1989)

Left of (cM)

kar

(Reuter et al., 1986)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

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Gene Model & Products

Please see the GBrowse view of Dmel\Pp1-87B for information on other features

detailed view

Comments on Gene Model

<new comment>Unusually long noncoding 3' UTR.

DGC clone LP06778 appears problematic: incomplete CDS

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Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

FBtr0082595

2211

302

Additional Transcript Data & Comments

Reported size (kB)

2.5, 1.6 (northern blot)

(Dombradi et al., 1989)

Comments

External Data

Crossreferences

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Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Pp1-87B-PA

FBpp0082067

34.5

302

5.26

NP_524937

AAF54810

Additional Polypeptide Data & Comments

Reported size (kD)

302 (aa); 34.5 (kD predicted)

(Dombradi et al., 1989)

Comments

External Data

PANTHER - Protein classification by function, families, and pathways

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FBgn0004103

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Metallophosphoesterase (IPR004843)

Serine/threonine-specific protein phosphatase and bis(5-nucleosyl)-tetraphosphatase (IPR006186)

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Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

UniProtKB/Swiss-Prot

P12982

UniProtKB/TrEMBL

Maps to

(Deak et al., 1997)

Does NOT map to

(Deak et al., 1997)

Identified with

LD03380

(BDGP Project Members, 2000-)

(Spradling et al., 1999)

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Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

aberration junction

Pp1-87B[87Bg-3].bk
3R:8,251,575..8,251,575

comment=Deletion with a breakpoint 165bp upstream of the ATG initiation site. This removes the TATA box and other upstream sequences. Extent of deletion unknown.
evidence=experimental

(Dombradi et al., 1990)

point mutation

Pp1-87B[1]
3R:8,250,753..8,250,753

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G8250753A
pr_change=G220S|Pp1-87B-PA
reported_pr_change=G220S

(Dombradi and Cohen, 1992)

point mutation

Pp1-87B[87Bg-1]
3R:8,250,752..8,250,752

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=G8250752A
pr_change=G220D|Pp1-87B-PA
reported_pr_change=G220D

(Dombradi and Cohen, 1992)

rescue fragment

Pp1-87B[+t5.8]
3R:8,248,077..8,253,916

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-EcoRI_rfrag

(Baksa et al., 1993)

rescue fragment

Pp1-87B[+t6.5]
3R:8,247,304..8,253,916

comment=Position of restriction fragment on reference sequence inferred by FlyBase curator.
evidence=experimental
linked_to=BamHI-BamHI_rfrag

(Axton et al., 1990)

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External Data

DEDB - Drosophila exon database: splicing graphs

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9180

Crossreferences

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Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

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Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage-adult stage

(Dombradi et al., 1989)

Marker for

Subcellular Localization

CV Term

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Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

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External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0004103

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0004103

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Alleles & Phenotypes

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Summary of Allele Phenotypes

Lethality

Allele

Pp1-87B^87Bg-1

lethal (with Pp1-87B¹)

Pp1-87B^87Bg-3

lethal (with Pp1-87B^87Bg-3)

Pp1-87B¹

lethal | recessive

semi-lethal (with Pp1-87B¹)

Pp1-87B^87Bg-3

semi-lethal (with Pp1-87B^87Bg-3)

Pp1-87B¹

semi-lethal | recessive

Pp1-87B¹

Other Phenotypes

Allele

suppressor of variegation

Pp1-87B^87Bg-1

suppressor of variegation | dominant, with In(1)w^m4h

suppressor of variegation, with In(1)w^m4

Pp1-87B¹

suppressor of variegation, with In(1)w^m4h

Pp1-87B¹

suppressor of variegation, with In(2R)bw^VDe2

Pp1-87B¹

suppressor of variegation, with Byron

Pp1-87B¹

suppressor of variegation, with P{wA^R}41A

Pp1-87B¹

non-suppressor of variegation, with P{wA^R}w^A

Pp1-87B¹

mitotic cell cycle defective

Phenotype manifest in

Allele

ganglion mother cell

nuclear chromosome

Pp1-87B^87Bg-1

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-6)

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-6)

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-6)

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-6)

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-6)

larval optic neuropil (with Pp1-87B^87Bg-6)

mitosis & nuclear chromosome

Pp1-87B^87Bg-3

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

larval optic neuropil (with Pp1-87B^87Bg-3)

Pp1-87B^87Bg-6

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

larval optic neuropil (with Pp1-87B^87Bg-1)

Pp1-87B^87Bg-6

photoreceptor cell R1 & axon, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

photoreceptor cell R2 & axon, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

photoreceptor cell R3 & axon, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

photoreceptor cell R4 & axon, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

photoreceptor cell R5 & axon, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

larval optic neuropil, with Scer\GAL4^GMR.PF

Pp1-87B^{Scer\UAS.T:Ivir\HA1}

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Classical Alleles ( 14 )

For All Classical Alleles Show

Allele of Pp1-87B	Class	Mutagen	Stocks	Known lesion
Pp1-87B¹³¹¹		X ray	0	--
Pp1-87B¹⁴⁵⁵		X ray	0	--
Pp1-87B¹	hypomorph	ethyl methanesulfonate	2	Yes
Pp1-87B²		X ray	1	--
Pp1-87B^87Bg-1	hypomorph	ethyl methanesulfonate	0	Yes
Pp1-87B^87Bg-2		ethyl methanesulfonate	0	--
Pp1-87B^87Bg-3	amorph	ethyl methanesulfonate	2	Yes
Pp1-87B^87Bg-4		ethyl methanesulfonate	0	--
Pp1-87B^87Bg-5		ethyl methanesulfonate	0	--
Pp1-87B^87Bg-6	amorph	ethyl methanesulfonate	0	Yes
Pp1-87B^BG00793		P-element activity	1	--
Pp1-87B^DG29605		P-element activity	1