Gene Dmel\W

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General Information

Symbol

Dmel\W

Species

D. melanogaster

Name

Wrinkled

Annotation symbol

CG5123

Feature type

protein_coding_gene

FlyBase ID

FBgn0003997

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

75C1-75C1

Sequence location

3L:18,160,842..18,178,737 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Wrinkled is referred to in FlyBase by the symbol W (CG5123, FBgn0003997). It has the cytological map location 75C1. Its sequence location is 3L:18160842..18178737. Its molecular function is unknown. It is involved in the biological processes described with 21 unique terms, many of which group under: anatomical structure development; programmed cell death; cell death; positive regulation of programmed cell death; embryonic development via the syncytial blastoderm; regulation of developmental process; response to light stimulus; cellular macromolecule metabolic process; sensory organ development; embryonic development ending in birth or egg hatching. 56 alleles are reported. The phenotypes of these alleles are annotated with 37 unique terms, many of which group under: organ system; nervous system; peripheral nervous system; embryonic nervous system; adult segment; cephalopharyngeal skeleton; antennal segment; adult; imaginal precursor; appendage segment. It has one annotated transcript and one annotated polypeptide.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

W: Wrinkled

Homozygote viable. Wings remain small and unexpanded. Black spots on head beside proboscis or ocelli. Heterozygous female like homozygote but less extreme. Male much less extreme; wings often expanded but wrinkled, blistered, and surface finely pebbled and grayish; no overlap with wild type. Suppressed by D in male and nearly so in female. From prepupal stage through adult, wing bases abnormally narrow, possibly preventing flow of body fluid in sufficient quantity to expand wings [Waddington, 1940, J. Genet. 41: 75-139 (fig.)]. RK1 as dominant.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

75C1-75C1

Limits computationally determined from genome sequence between P{lacW}l(3)j11B2^j11B2 and P{PZ}W⁰⁵⁰¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

75C1-75C2

(Grether, 1995.7.7)

75C1-75C2

(determined by in situ hybridisation)

(Spradling et al., 1999)

75C1-75C2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Aph-1

(Wallis and Fox, 1968)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: W- CG7320? CheA75a? CG5103? CG13700? grim- rpr- skl-

(Wing et al., 2002)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: W? CG7320? CheA75a? CG5103? CG13700? grim? rpr? skl?

(Srinivasula et al., 2002)

Gene Model & Products

Please see the GBrowse view of Dmel\W for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

W-RA

FBtr0075133

NM_079412

4017

410

Additional Transcript Data & Comments

Reported size (kB)

4.2 (northern blot)

(Grether et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

W-PA

FBpp0074899

43.9

410

8.35

NP_524136

AAF49270

Additional Polypeptide Data & Comments

Reported size (kD)

410 (aa); 43 (kD predicted)

(Grether et al., 1995)

Comments

External Data

Linkouts

Crossreferences

PDB - Protein Data Bank. An information portal to biological macromolecular structures

•

1JD6

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q24106

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

AT13267

(BDGP Project Members, 2000-)

(Wing et al., 2002)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

deletion

W[hid-ML66]
3L:18,165,159..18,165,159

comment=Splice donor mutation at junction between exons 3 and 4. Deletion of the G residue.
evidence=experimental

(Grether et al., 1995)

point mutation

W[hid-A22]
3L:18,166,475..18,166,475

evidence=experimental
na_change=C18166475T
pr_change=P171S|W-PA
reported_na_change=C910T
reported_pr_change=P170S

(Grether et al., 1995)

point mutation

W[hid-A206]-1
3L:18,166,204..18,166,204

comment=One of two amino acid changes observed in W[hid-A206].
evidence=experimental
na_change=C18166204T
pr_change=S261L|W-PA
reported_na_change=C1181T
reported_pr_change=S261L

(Grether et al., 1995)

point mutation

W[hid-A206]-2
3L:18,166,166..18,166,166

comment=One of two amino acid changes observed in W[hid-A206]
evidence=experimental
na_change=C18166166T
pr_change=Q274@|W-PA
reported_na_change=C715T
reported_pr_change=Q274@

(Grether et al., 1995)

point mutation

W[hid-A329]
3L:18,165,409..18,165,409

comment=TGG to TGA
evidence=experimental
na_change=G18165409A
pr_change=W301@|W-PA
reported_na_change=G1302A
reported_pr_change=W301@

(Grether et al., 1995)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3672

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 17

midline glial cell

(Bergmann et al., 2002)

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI257580

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003997

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0003997

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0003997

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable, with Scer\GAL4^rdgC.PK

W^Scer\UAS.cZa

lethal

W^hs.PG

lethal | recessive

W⁰⁵⁰¹⁴

lethal | female limited, with Tn10\tetR^Yp1.hs

W^{Tn10\tetO.cHa}

lethal | conditional ts

W^hs.PG

lethal | embryonic stage (with Df(3L)H99)

W⁰⁵⁰¹⁴

lethal | embryonic stage | recessive

lethal | embryonic stage, with Scer\GAL4^tim.PE

W^Scer\UAS.cZa

lethal | pupal stage, with Scer\GAL4^GMR.PF

W^Scer\UAS.cZa

lethal, with Scer\GAL4^GMR.PF

W^Scer\UAS.cZa

lethal, with Scer\GAL4^Act5C

W^GD1673

semi-lethal

W¹¹⁰

semi-lethal | larval stage, with Scer\GAL4^Eh.2.4

W^Scer\UAS.cZa

Other Phenotypes

Allele

thermotaxis behavior defective, with Scer\GAL4^TrpA1.PR

W^Scer\UAS.cZa

visible

visible | dominant

W^GMR.PG

visible | conditional RU486, with Scer\GAL4^{GMR.in.Switch.PR}

W^Scer\UAS.cZa

visible | somatic clone

W^GMR.PG

visible, with Scer\GAL4^GMR.PF

W^Scer\UAS.cZa

behavioral | larval stage | recessive

W^GMR.PG

locomotor rhythm defective

W^GMR.PG

locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf

W^Scer\UAS.cZa

locomotor rhythm defective, with Scer\GAL4^cry.PE

W^Scer\UAS.cZa

locomotor rhythm defective, with Scer\GAL4^cry.PE, Scer\GAL80^Pdf.PS

W^Scer\UAS.cZa

eclosion rhythm defective, with Scer\GAL4^P2.4.Pdf

W^Scer\UAS.cZa

phototaxis behavior defective | larval stage

W^GMR.PG

locomotor behavior defective | larval stage

W^GMR.PG

flightless | dominant

W^unspecified

cell death defective

cell death defective | conditional cs

W^dsRNA.hs

cell death defective | somatic clone, with Scer\GAL4^Act5C.PP

W^Scer\UAS.cCa

cell death defective, with Scer\GAL4^Gp150-52A

W^Scer\UAS.cZa

cell death defective, with Scer\GAL4^Eh.2.4

W^Scer\UAS.cZa

cell death increase

cell death increase, with Scer\GAL4^ptc-559.1

W^EP30060

cell death increase, with Scer\GAL4^GMR.PF

W^Scer\UAS.cZa

cell death increase, with Scer\GAL4^Rh5.PT

W^Scer\UAS.cZa

cell death increase, with Scer\GAL4^Rh6.PD

W^Scer\UAS.cZa

cell death decrease

cell death decrease (with W⁰⁵⁰¹⁴)

W^rvX1

cell death decrease (with W^rvX1)

W⁰⁵⁰¹⁴

cell death decrease | conditional ts

W^dsRNA.hs

neuroanatomy defective

W^GMR.PG

neuroanatomy defective, with Scer\GAL4^Rh6.PD

W^Scer\UAS.cZa

chemical resistant, with Scer\GAL4^P2.4.Pdf

W^Scer\UAS.cZa

Phenotype manifest in

Allele

spermatid (with Df(3L)H99)

W⁰⁵⁰¹⁴

arista lateral | ectopic (with Df(3L)H99)

arista (with Df(3L)H99)

eye disc (with W^rvX1)

W⁰⁵⁰¹⁴

ommatidial cluster (with W^rvX1)

W⁰⁵⁰¹⁴

dorsal closure embryo

ventral nerve cord commissure, with Scer\GAL4^sli.PS

W^{Ala5.Scer\UAS}

midline glial cell, with Scer\GAL4^sli.PS

W^{Ala5.Scer\UAS}

embryonic hemocyte, with Scer\GAL4^srp.Hemo

W^{Ala5.Scer\UAS}

ommatidium

photoreceptor cell

W^GMR.PG

larval Bolwig's organ

W^GMR.PG

eye disc

W^GMR.PG

eye | somatic clone

W^GMR.PG

larval lateral neuron

W^GMR.PG

Bolwig's nerve

W^GMR.PG

serotonin neuron

W^GMR.PG

interommatidial bristle

W^GMR.PG

eye, with Scer\GAL4^GMR.PF

follicle cell | somatic clone, with Scer\GAL4^Act5C.PP

W^Scer\UAS.cCa

nurse cell | somatic clone, with Scer\GAL4^Act5C.PP

W^Scer\UAS.cCa

follicle cell & nucleus | somatic clone, with Scer\GAL4^Act5C.PP

W^Scer\UAS.cCa

eye | conditional RU486, with Scer\GAL4^{GMR.in.Switch.PR}

W^Scer\UAS.cZa

pigment cell, with Scer\GAL4^{GMR.in.Switch.PR}

W^Scer\UAS.cZa

eye, with Scer\GAL4^eya.PB

W^Scer\UAS.cZa

ventral midline glia, with Scer\GAL4^Gp150-52A

W^Scer\UAS.cZa

serotonin neuron, with Scer\GAL4^Rh6.PD

W^Scer\UAS.cZa

photoreceptor cell R6, with Scer\GAL4^Rh6.PD

W^Scer\UAS.cZa

photoreceptor cell R5, with Scer\GAL4^Rh5.PT

W^Scer\UAS.cZa

embryonic/larval trachea | dorsal, with Scer\GAL4^Eh.2.4

W^Scer\UAS.cZa

neuron & larva, with Scer\GAL4^Eh.2.4

W^Scer\UAS.cZa

embryonic head | conditional ts

W^dsRNA.hs

larval salivary gland & pupa | conditional ts

W^dsRNA.hs

tracheal metamere 2 | conditional cs

W^dsRNA.hs

male terminalia

cephalopharyngeal skeleton

gonadal sheath proper primordium | female (with Df(3L)H99)

W^hid-A206

latticed process

W^hid-H109

embryo

W^hs.PG

eye disc (with W⁰⁵⁰¹⁴)

W^rvX1

ommatidial cluster (with W⁰⁵⁰¹⁴)

W^rvX1

midline glial cell

long arista lateral (with Df(3L)H99)

W^rvX1

embryonic brain

W^unspecified

wing, with Scer\GAL4^Bx-MS1096

W^GD1673

thorax, with Scer\GAL4^pnr-MD237

W^GD1673

Classical Alleles ( 30 )

For All Classical Alleles Show

Allele of W	Class	Mutagen	Stocks	Known lesion
W⁰⁵⁰¹⁴	amorph	P-element activity	0	Yes
W¹¹⁰		P-element activity	0	Yes
W¹		stratosphere exposure	13	--
W^40C		Δ2-3	0	Yes
W^8D		Δ2-3	0	Yes
W^DG09507			1	--
W^EP30060		P-element activity	0	--
W^c01591		piggyBac transposase	1	--
W^c01693		piggyBac transposase	1	--
W^c04917		piggyBac transposase	1	--
W^d05449		P-element activity	1	--
W^f07651		piggyBac transposase	1	--
W^hid-A206	hypomorph	ethyl methanesulfonate	0	Yes
W^hid-A22	hypomorph	X ray	0	Yes
W^hid-A329	hypomorph	ethyl methanesulfonate	0	Yes
W^hid-H109	amorph	γ ray	0	--
W^hid-H89	hypomorph	γ ray	0	--
W^hid-ML66			0	Yes
W^k05017			0	--
W^rv16		X ray	0	--
W^rv19		X ray	0	--
W^rvE1		ethyl methanesulfonate	0	--
W^rvE2		ethyl methanesulfonate	0	--
W^rvE3		ethyl methanesulfonate	0	--
W^rvE4		ethyl methanesulfonate	0	--
W^rvE5		ethyl methanesulfonate	0	--
W^rvE6		ethyl methanesulfonate	0	--
W^rvX1		X ray	0	Yes
W^rvX2		X ray	0	--
W^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 26 )

For All Alleles Carried on Transgenic Constructs Show

Allele of W	Mutagen	Stocks	Known lesion
W^{Ala1.MtnA.T:Scer\Ubi4}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion in vitro construct \| site directed mutagenesis	0	Yes
W^Ala3.GMR	in vitro construct \| site directed mutagenesis in vitro construct \| amino acid replacement in vitro construct \| regulatory fusion	0	Yes
W^Ala3	in vitro construct \| site directed mutagenesis in vitro construct \| amino acid replacement	0	Yes
W^Ala5.GMR	in vitro construct \| site directed mutagenesis in vitro construct \| amino acid replacement in vitro construct \| regulatory fusion	0	Yes
W^{Ala5.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| site directed mutagenesis	0	Yes
W^Ala5	in vitro construct \| site directed mutagenesis in vitro construct \| amino acid replacement	0	Yes
W^GD1673	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
W^GMR.PA	in vitro construct \| regulatory fusion	0	Yes
W^GMR.PG	in vitro construct \| regulatory fusion	7	Yes
W^GMR.PH	in vitro construct \| regulatory fusion	0	Yes
W^GMR.PHb	Δ2-3	0	Yes
W^MtnA.PC	in vitro construct \| regulatory fusion	0	Yes
W^MtnA.PY	in vitro construct \| regulatory fusion	0	Yes
W^{MtnA.T:SV5\V5}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
W^{MtnA.T:Scer\Ubi4}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion in vitro construct \| site directed mutagenesis	0	Yes
W^{MtnA.T:Zzzz\TAP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
W^Scer\UAS.cCa	in vitro construct \| regulatory fusion	0	Yes
W^Scer\UAS.cIa	in vitro construct \| regulatory fusion	0	Yes <