Home

A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008

Gene Dmel\αTub85E

Close All Open All

General Information

Symbol

Dmel\αTub85E

Species

D. melanogaster

Name

α-Tubulin at 85E

Annotation symbol

CG9476

Feature type

protein_coding_gene

FlyBase ID

FBgn0003886

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

3R

Recombination map

Cytogenetic map

85E6-85E6

Sequence location

3R:5,556,938..5,558,970 [-]

Map ( GBrowse )

detailed view

hide

Summary Information

Automatically generated summary

See sections below for more information

The gene α-Tubulin at 85E is referred to in FlyBase by the symbol αTub85E (CG9476, FBgn0003886). It has the cytological map location 85E6. Its sequence location is 3R:5556938..5558970. Its molecular function is described as: structural constituent of cytoskeleton; GTP binding; structural molecule activity; GTPase activity. It is involved in the biological processes: mitotic cell cycle spindle assembly checkpoint; cellular process; mitotic spindle organization and biogenesis; microtubule-based movement; protein polymerization. 3 alleles are reported. The phenotype of these alleles is annotated with spermatid. It has one annotated transcript and one annotated polypeptide.

hide

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

αTub85E (α2t)

A structural gene for α-Tubulin. Only α-Tubulin not expressed maternally. Transcripts present from 6-8 hr through adult. Protein found in support cells of chordotonal organs, developing muscles, and somatic component of the testis (Matthews, Miller, and Kaufman).

hide

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85E6-85E6

Limits computationally determined from genome sequence between P{EP}Crc^EP3006 and P{lacW}s2681

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

85E-85E

(determined by in situ hybridisation)

(Drosopoulou and Scouras, 1998)

85E6-85E10

(determined by in situ hybridisation)

(Natzle and McCarthy, 1984)

85E6-85E10

(determined by in situ hybridisation)

(Mischke and Pardue, 1982)

85E6-85E10

(determined by in situ hybridisation)

(Kalfayan and Wensink, 1981)

85E6-85E10

(determined by in situ hybridisation)

(Sanchez et al., 1980)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

hide

Gene Model & Products

Please see the GBrowse view of Dmel\αTub85E for information on other features

detailed view

Comments on Gene Model

hide

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

FBtr0082087

1563

449

Additional Transcript Data & Comments

Reported size (kB)

1.85, 1.65 (northern blot)

(Kalfayan and Wensink, 1982)

2.0, 1.8 (northern blot)

(Natzle and McCarthy, 1984)

2.049 (sequence analysis)

(Theurkauf et al., 1986)

1.55 (northern blot)

(Matthews et al., 1989)

Comments

External Data

Crossreferences

hide

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

αTub85E-PA

FBpp0081565

50.0

449

4.83

NP_524297

AAF54433

Additional Polypeptide Data & Comments

Reported size (kD)

449 (aa); 55 (kD predicted)

(Theurkauf et al., 1986)

Comments

Polypeptides were identified in wing imaginal discs and in the CME W2 wing imaginal disc cell line by 2D gel electrophoresis and by microsequencing.

(Santaren et al., 1993)

External Data

PANTHER - Protein classification by function, families, and pathways

•

FBgn0003886

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tubulin (IPR000217)

Alpha tubulin (IPR002452)

Tubulin/FtsZ, GTPase (IPR003008)

Tubulin/FtsZ, C-terminal (IPR008280)

hide

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

UniProtKB/Swiss-Prot

P06604

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(Oliver, 2001.8.16)

RH71862

(BDGP Project Members, 2000-)

hide

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

hide

External Data

DEDB - Drosophila exon database: splicing graphs

•

8836

Crossreferences

hide

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

hide

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage | third instar

(Natzle and McCarthy, 1984)

embryonic stage | >=8-12 hr

(Matthews et al., 1989)

pupal stage

(Natzle and McCarthy, 1984)

larval stage | second instar

(Natzle and McCarthy, 1984)

larval stage-pupal stage

(Matthews et al., 1989)

embryonic stage | 0-6 hr

(Natzle and McCarthy, 1984)

larval stage | first instar

(Natzle and McCarthy, 1984)

adult stage | male

(Kalfayan and Wensink, 1982)

larval stage

(Kalfayan and Wensink, 1982)

embryonic stage | 6-12 hr

(Kalfayan and Wensink, 1982)

prepupal stage

(Natzle and McCarthy, 1984)

adult stage,spermatogenesis

testis

(Matthews et al., 1989)

larval stage

(Kalfayan and Wensink, 1982)

embryonic stage | 2-21 hr

(Natzle and McCarthy, 1984)

Marker for

Subcellular Localization

CV Term

hide

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

larval stage

imaginal disc

(Matthews et al., 1989)

embryonic stage | 16-20 hr

(Matthews et al., 1989)

larval stage | third instar

dorsal mesothoracic disc

(Santaren et al., 1993)

adult stage,spermatogenesis

testis

(Matthews et al., 1989)

Marker for

Subcellular Localization

CV Term

hide

External Data & Images

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003886

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0003886

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0003886

hide

Alleles & Phenotypes

hide

Summary of Allele Phenotypes

Sterility

Allele

αTub85E^βTub85D.PH

Phenotype manifest in

Allele

αTub85E^βTub85D.PH

hide

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of αTub85E	Class	Mutagen	Stocks	Known lesion
αTub85E^unspecified			0	--

hide

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of αTub85E	Class	Mutagen	Stocks	Known lesion
αTub85E^GD14226		in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
αTub85E^βTub85D.PH		in vitro construct \| regulatory fusion	0	Yes

hide

Aneuploid Aberrations

Useful deficiency

Df(3R)GB104

Useful duplication

Dp(3;3)M86D⁺2

Disrupted in

Df(3R)GB104

(Seshaiah et al., 2001)

Df(3R)by10

(Seshaiah et al., 2001)

Tp(3;2)by62

(Seshaiah et al., 2001)

hide

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

Yes

No

Insertions

Type of insertions

Name

Expression data

hide

Related Comments

Please look at the allele reports for the complete phenotype data

Identified in 2D gels of CMW W2 wing imaginal disc cell proteins.

(Santaren et al., 1993)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

hide

Gene Ontology: Function, Process & Cellular Component ( 12 )

hide

Molecular Function

CV term

References

non-traceable author statement

(Swiss-Prot Project Members, 1988.1.1)

inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR002452, InterPro:IPR008280

(FlyBase Curators et al., 2004-)

GTPase activity

inferred from electronic annotation with InterPro:IPR008280

(FlyBase Curators et al., 2004-)

structural constituent of cytoskeleton

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

structural molecule activity

inferred from electronic annotation with InterPro:IPR002452

(FlyBase Curators et al., 2004-)

hide

Biological Process

CV term

References

cellular process

inferred from mutant phenotype

(Boutros et al., 2004)

microtubule-based movement

inferred from electronic annotation with InterPro:IPR002452

(FlyBase Curators et al., 2004-)

mitotic cell cycle spindle assembly checkpoint

inferred from direct assay

(Logarinho et al., 2004)

mitotic spindle organization and biogenesis

inferred from mutant phenotype

(Goshima et al., 2007)

protein polymerization

inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280

(FlyBase Curators et al., 2004-)

hide

Cellular Component

CV term

References

inferred from sequence or structural similarity

(Goldstein and Gunawardena, 2000)

non-traceable author statement

(Swiss-Prot Project Members, 1988.1.1)

inferred from electronic annotation with InterPro:IPR000217, InterPro:IPR002452

(FlyBase Curators et al., 2004-)

protein complex

inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280

(FlyBase Curators et al., 2004-)

tubulin complex

non-traceable author statement

(Swiss-Prot Project Members, 1988.1.1)

hide

Sequence Ontology: Class of Gene

protein_coding_gene

hide

Interactions & Pathways

hide

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

αTub85E allele

Gene

References

αTub85E^βTub85D.PH

(Hoyle et al., 2001)

hide

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66342

hide

Orthologs

Genome-wide drosophilid orthologs

Dana\GF11529

Dere\GG17337

Dmoj\GI23620

Dsec\GM26223

Dsim\GD20767

Dyak\GE24741

Curated drosophilid orthologs

Daur\αTub85E

Dbio\αTub85E

Djam\αTub85E

Dmau\αTub85E

(Zeng et al., 2000)

Dpse\GA21818

Dseg\αTub85E

Dser\αTub85E

Dsim\αTub85E

(Zeng et al., 2000)

InParanoid orthologs - Eukaryotic orthologs

•

DDB0191380
Dictyostelium discoideum (Dictybase)

hide

Functional Complementation between Species

hide

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

hide

Stocks & Reagents

hide

Stocks Listed in FlyBase ( 0 )

hide

Genomic Clones ( 2 )

hide

cDNA Clones ( 29 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RH71862

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

hide

RNAi & Array Information

Affy Oligo

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0003886

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0003886

hide

Antibody Information

hide

Other Information

hide

Discoverer

hide

Etymology

hide

Identification

hide

Position Effect Variegation Data

hide

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

hide

Comments About Role

In D.melanogaster, two multigene families, each made up of four members, code for α- and β-tubulins. Tubulins are a highly conserved family of proteins that are the main structural components of microtubules in mitotic and meiotic spindles, cilia, flagella, neural processes and the cytoskeleton; nontubulin proteins (MAPS or microtubule-associated proteins) are involved along with tubulins in the formation of specialized microtubules (FBrf0045282; FBrf0046966). αTub85E is the only α-tubulin not expressed maternally. Transcripts present from 6-8 hr through adult. Protein found in support cells of chordotonal organs, developing muscles and somatic component of the testis (FBrf0049502). In adults expression of αTub85E is male-specific (FBrf0045282).

The expression of βTub60D is accompanied by a coordinate transient increase in the level of synthesis of the embryonic α-tubulins, thereby maintaining an approximately equimolar synthesis of α- and β-tubulins throughout embryogenesis.

(Raff et al., 1982)

The tissue specific pattern of αTub85E protein accumulation is identified by isotope specific antibody.

(Matthews et al., 1990)

hide

Comments About Molecular Function

hide

Other Comments

Though αTub84B and αTub85E are very similar in sequence they are functionally distinct. αTub85E can provide function in the male germ line fr generic microtubule arrays but lacks a subset of the functional properties possessed by necessary for specific features of microtubule assembly unique to the male germ cells.

(Hutchens et al., 1997)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection acting against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

hide

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079573

RefSeq (Proteins)

•

NP_524297

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

P06604

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tubulin (IPR000217)

Alpha tubulin (IPR002452)

Tubulin/FtsZ, GTPase (IPR003008)

Tubulin/FtsZ, C-terminal (IPR008280)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

66342

DEDB - Drosophila exon database: splicing graphs

•

8836

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0003886

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003886

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0003886

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0003886

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0003886

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0003886

InParanoid orthologs - Eukaryotic orthologs

•

DDB0191380
Dictyostelium discoideum (Dictybase)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0003886

hide

Synonyms & Secondary IDs ( 29 )

Reported As

Symbol Synonym

2t

(Fyrberg and Goldstein, 1990)

a2t

agr;Tub

(Audibert et al., 2005)

ALPHA 85E

(Goldstein and Gunawardena, 2000)

aTub85E

(Kliman and Eyre-Walker, 1998)

CG9476

(Goldstein and Gunawardena, 2000, Goshima et al., 2007)

DTA3

(Natzle and McCarthy, 1984)

T

(Courgeon et al., 1993)

Tub

(Harris and Peifer, 2004)

α2

(Drosopoulou and Scouras, 1998, Bo and Wensink, 1989)

α2t

α85E

(Hoyle et al., 2001, Raff, 1984)

α85E-tub

(Inbal et al., 2004)

α-Tub

(Ruel et al., 2003)

αTub2

(Mansfield et al., 1994, )

αTub84E

α-Tub85E

αTub85E

(Goshima et al., 2007)

α-tubulin

(Martinez-Campos et al., 2004, Wasser and Chia, 2003, Maiato et al., 2006)

Name Synonym

alpha-tubulin

(Przewloka, 2007)

tubulin

(Harris and Peifer, 2004, Galindo et al., 2007, Harris and Peifer, 2005, Goshima et al., 2005, Ashley et al., 2005)

α2-tubulin

(Bo and Wensink, 1989)

α2tubulin

(Raff et al., 1982)

α85E-tubulin

(Dettman et al., 2001)

αT

(Galindo et al., 2007)

α-Tubulin

(Betschinger et al., 2005, Berdnik and Knoblich, 2002, van Steensel et al., 2001, Brandt et al., 2006)

α-tubulin

(Kwon and Scholey, 2004, Cottam et al., 2004, Fuse et al., 2003, Betschinger et al., 2003, Mollinari et al., 2002, Sampaio et al., 2001, Bellaiche et al., 2001, Wakefield et al., 2001, Walker et al., 2000, Ben-Yaacov et al., 2001, Leismann et al., 2000, Roos et al., 2000, Lemos et al., 2000, Lange et al., 2000, Wilson, 1999, Wilson, 1999, Gho et al., 1999, Herrmann et al., 1998, Kuchinke et al., 1998, Riparbelli et al., 1997, Eaton et al., 1996, Brill et al., 1995, Endow et al., 1994, Callaini et al., 1994, Baker et al., 1993, Wu et al., 1990, Logarinho et al., 2004, Cenci et al., 2003, Somma et al., 2003, Roper and Brown, 2004, Mendjan et al., 2006, Verollet et al., 2006, Goshima et al., 2007, Wilson, 2005, McHugh et al., 2004, Murray and Saint, 2007, Ohlstein and Spradling, 2007, Rujano et al., 2006, Sakaguchi and Steward, 2007, Meignin et al., 2004, Cullen et al., 2005, Cheng et al., 2008, Holtzman et al., 2008, Gao et al., 2008, Wang et al., 2007)

αtubulin

(Battaglia et al., 2001, Grieder and Spradling, 1999)

α tubulin

(Donaldson et al., 2001, Audibert and Simonelig, 1999, Nicolai et al., 1999, Avides and Glover, 1999,