Gene Dmel\T-cp1

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General Information

Symbol

Dmel\T-cp1

Species

D. melanogaster

Name

Tcp1-like

Annotation symbol

CG5374

Feature type

protein_coding_gene

FlyBase ID

FBgn0003676

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

94B1-94B1

Sequence location

3R:18,314,807..18,317,153 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Tcp1-like is referred to in FlyBase by the symbol T-cp1 (CG5374, FBgn0003676). It has the cytological map location 94B1. Its sequence location is 3R:18314807..18317153. Its molecular function is described as: hydrogen-exporting ATPase activity, phosphorylative mechanism; unfolded protein binding; ATP binding. It is involved in the biological processes: protein folding; phagocytosis, engulfment; mitotic spindle organization and biogenesis. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

T-cp1: T-complex 1

Codes for a cDNA from Drosophila melanogaster that shares 66% sequence identity with the mouse Tcp-1 gene encoding TCP1 (t-complex polypeptide 1).

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B1-94B1

Limits computationally determined from genome sequence between P{PZ}l(3)rQ178^rQ178 and P{lacW}Dph5^L4910

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

94B1-94B2

(determined by in situ hybridisation)

(Ursic and Ganetzky, 1988)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\T-cp1 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

T-cp1-RA

FBtr0084291

NM_079726

1992

557

T-cp1-RB

FBtr0084290

NM_170016

1928

557

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

T-cp1-PA

FBpp0083684

59.6

557

6.30

NP_524450

AAF56009

T-cp1-PB

FBpp0083683

59.6

557

6.30

NP_732748

AAN13906

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0003676

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Chaperonin Cpn60 (IPR001844)

Chaperonin TCP-1, conserved site (IPR002194)

Chaperonin Cpn60/TCP-1 (IPR002423)

GroEL-like chaperone, ATPase (IPR008950)

T-complex protein 1, alpha subunit (IPR012715)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P12613

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

(Oliver, 2001.8.16)

RE70560

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10337

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI113861

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0003676

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0003676

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for T-cp1

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for T-cp1

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of T-cp1	Class	Mutagen	Stocks	Known lesion
T-cp1^c01712		piggyBac transposase	0	--
T-cp1^c03987		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of T-cp1	Class	Mutagen	Stocks	Known lesion
T-cp1^GD10468		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)hh

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)Exel6191

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10468}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{PB}T-cp1^c01712

PBac{PB}T-cp1^c03987

Related Comments

Please look at the allele reports for the complete phenotype data

Codes for a cDNA from D.melanogaster that shares 66% sequence identity with the mouse Tcp-1 gene encoding TCP1 (t-complex polypeptide 1).

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

ATP binding

non-traceable author statement

(Swiss-Prot Project Members, 1989.10.1)

inferred from electronic annotation with InterPro:IPR001844, InterPro:IPR002194, InterPro:IPR002423, InterPro:IPR008950, InterPro:IPR012715

(FlyBase Curators et al., 2004-)

hydrogen-exporting ATPase activity, phosphorylative mechanism