A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\Ste

General Information
SymbolDmel\SteSpeciesD. melanogaster
NameStellateAnnotation symbol
Feature typeprotein_coding_geneFlyBase IDFBgn0003523
Created / Updated2006-05-14/2006-05-14
Genomic Location
Chromosome (arm)Recombination map1-45.7
Cytogenetic mapSequence location
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Stellate is referred to in FlyBase by the symbol Ste (FBgn0003523). It has not been localized to the genome sequence. Its molecular function is described as protein kinase CK2 regulator activity. It is involved in the biological process spermatogenesis. 9 alleles are reported. The phenotype of these alleles is annotated with spermatocyte. It has no annotated transcripts.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
Ste: Stellate
This locus is responsible for the appearance of crystals in the nuclei and cytoplasm of primary spermatocytes of XO males. Enzymatic treatments indicate that these crystals are proteinaceous in nature (Meyer et al.). The suppression of crystal formation by the Y chromosome is attributable to a sequence designated Su(Ste).
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
12E1-12E2  
Left limit from inclusion within Df(1)R12.1 (FBrf0079138) Right limit from in situ hybridisation (FBrf0079138)  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
12E1-12E2
(determined by in situ hybridisation)  
(Palumbo et al., 1994)
12D1-12F2
(determined by in situ hybridisation)  
(Heino, 1994)
Experimentally Determined Recombination Data
Location
1-45.7
(Hardy et al., 1984)
Left of (cM)
(Hardy et al., 1984)
Right of (cM)
(Hardy et al., 1984)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: tRNA:S4:12Ea+ tRNA:S4:12Eb+ tRNA:S774:12Ec- tRNA:S7:12Ed- tRNA:S4:12Ee- tRNA:S774:12Ef- tRNA:S7:12Eg+ tRNA:S474:12Eh- Ste?
(Leung et al., 1991)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Casein kinase II, regulatory subunit (IPR000704)
Casein kinase II, regulatory subunit, alpha-helical (IPR016149)
Casein kinase II, regulatory subunit, beta-sheet (IPR016150)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AY118877
AAM50737
 
BI355674
 
X15899
CAA33906
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
(BDGP Project Members, 2000-)
(BDGP Project Members, 1994-1999)
hide Mapped Features & Mutations
Type
Symbol & Location
Additional Notes
References
hide External Data
Linkouts
Crossreferences
hide Expression Data
FlyBase-Curated Data
Transcript and
Protein data 		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
hide Summary of Transcript Expression
Stage
Tissue/Position
Reference
Marker for
    Subcellular Localization
    CV Term
    hide Summary of Polypeptide Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide External Data & Images
      Linkouts
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      •
      hide Alleles & Phenotypes
      hide Summary of Allele Phenotypes
      Lethality
      Allele
      viable
      Sterility
      Allele
      fertile
      Phenotype manifest in
      Allele
      spermatocyte
      hide Classical Alleles ( 4 )
      For All Classical Alleles Show

      Allele of SteClassMutagenStocksKnown lesion
      Ste1
        		3 --
        SteR301.21 Yes
        SteW120 Yes
        Steunspecified
          		0 --
          hide Alleles Carried on Transgenic Constructs ( 5 )
          For All Alleles Carried on Transgenic Constructs Show

          Allele of SteClassMutagenStocksKnown lesion
          Ste134.T:Ecol\lacZ0 Yes
          Ste225.T:Ecol\lacZ0 Yes
          SteALS.T:Scer\ilv20 Yes
          StedsRNA.cAa0 Yes
          Stehs.T:Ecol\lacZ0 Yes
          hide Aneuploid Aberrations
          Useful deficiency
           
          Duplicated in
          (Hardy et al., 1984, Palumbo et al., 1994)
          Disrupted in
          (Palumbo et al., 1994)
          hide Transgenic Constructs & Insertions
          Transgenic Constructs
          Type of construct
          Name
          Expression data
          characterization construct
          P{(Ste-ALS,ry+)}
          NA
          reporter construct
          P{Ste-lacZ.134}
          No
          P{Ste-lacZ.225}
          No
          Insertions
          Type of insertions
          Name
          Expression data
          miscellaneous insertions
          P{ry1}SteR301.2
          NA
          hide Related Comments
          Please look at the allele reports for the complete phenotype data
          This locus is responsible for the appearance of crystals in the nuclei and cytoplasm of primary spermatocytes of X0 males. Enzymatic treatments indicate that these crystals are proteinaceous in nature (Meyer, Hess and Beermann, 1961). The suppression of crystal formation by the Y chromosome is attributable to a sequence designated Su(Ste).
           
          The Ste locus contains two major size classes of a tandemly repeated gene. Analysis using segmental Y deficiencies shows that Su(Ste) represses both the high levels and efficient splicing of Ste RNA.
          (Livak, 1990)
          The relationship of Ste copy number and organisation to meiotic behaviour in Su(Ste)- males has been examined genetically and cytologically. Heterochromatic and euchromatic Ste repeats are functional, the abnormalities in chromosome condensation and frequency of nondisjunction is related to the Ste copy number. Meiosis is disrupted after synapsis and Su(Ste) induced meiotic drive is probably not mediated by Ste.
          (Palumbo et al., 1994)
          One of a class of genes with TATA-less promoters that have a subset of the conserved DPE sequence.
          (Burke and Kadonaga, 1996)
          Ste-/Su(Ste)- males have exactly the same meiotic drive phenotype as Ste+/Su(Ste)- males.
          (Belloni et al., 2002)
          hide Gene Ontology: Function, Process & Cellular Component ( 5 )
          hide Molecular Function
          CV term
          Evidence
          References
          protein kinase CK2 regulator activity
          inferred from sequence or structural similarity
          (Livak, 1990)
          non-traceable author statement
          (Swiss-Prot Project Members, 1990.4.1)
          inferred from electronic annotation with InterPro:IPR000704, InterPro:IPR016149, InterPro:IPR016150
          (FlyBase Curators et al., 2004-)
          hide Biological Process
          CV term
          Evidence
          References
          spermatogenesis
          traceable author statement
          (Livak, 1990)
          inferred from genetic interaction with FLYBASE:Su(Ste); FB:FBgn0003582
          (Palumbo et al., 1994)
          non-traceable author statement
          (Swiss-Prot Project Members, 1990.4.1)
          hide Cellular Component
          CV term
          Evidence
          References
          cytoplasm
          inferred from direct assay
          (Bozzetti et al., 1995)
          nucleus
          inferred from direct assay
          (Bozzetti et al., 1995)
          protein kinase CK2 complex
          non-traceable author statement
          (Swiss-Prot Project Members, 1990.4.1)
          inferred from electronic annotation with InterPro:IPR000704, InterPro:IPR016149, InterPro:IPR016150
          (FlyBase Curators et al., 2004-)
          hide Sequence Ontology: Class of Gene
          nuclear_gene
           
          gene_array
           
          protein_coding_gene
           
          hide Interactions & Pathways
          hide Summary of Genetic Interactions
          Interacts with
          Please look at the allele data for full details of the genetic interactions
          Ste allele
          Gene
          References
          Steunspecified
          aub
          (Tritto et al., 2003)
          unspecified
          sirio
          (Tritto et al., 2003)
          hide External Data
          Linkouts
          hide Orthologs
          Genome-wide drosophilid orthologs
          Curated drosophilid orthologs
          Linkouts
          hide Functional Complementation between Species
          hide Inter-Species Misexpression Data
          Produces phenotype in
          Produces NO phenotype in
          hide Stocks & Reagents
          hide Stocks Listed in FlyBase ( 4 )
          Bloomington
          10319
          P{ry1}SteR301.2; ry42
          3707
          y1 wa Ste1/Dp(1;Y)BSYy+
          2922
          T(1;Y)E1, y1 wa Ste1: y+ BS/C(1)*, y1 wa
          Kyoto
          107186
          y1 wa Ste1/Dp(1;Y)BSYy+
          hide Genomic Clones ( 0 )
          hide cDNA Clones ( 0 )
          cDNA Clones, Fully Sequenced
          BDGP DGC clones
          Other clones
          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          hide RNAi & Array Information
          Affy Oligo
          Linkouts
          hide Antibody Information
          hide Other Information
          hide Discoverer
          hide Etymology
          hide Identification
          hide Position Effect Variegation Data
          hide Relationship to Other Genes
          Source for database identity of
          Source for database merge of
          Source for merge of: Ste BcDNA:GM31840
          (FlyBase, 1996-)
          Component gene(s)
          Ste:CG33236
           
          Ste:CG33237
           
          Ste:CG33238
           
          Ste:CG33239
           
          Ste:CG33240
           
          Ste:CG33241
           
          Ste:CG33242
           
          Ste:CG33243
           
          Ste:CG33244
           
          Ste:CG33245
           
          Ste:CG33246
           
          Ste:CG33247
           
          Additional comments
          Annotation CG32605 split into 12 annotations, each representing a member of the Ste cluster, in release 3.2 of the genome annotation. The 12 annotations are: Ste:CG33236, Ste:CG33237, Ste:CG33238, Ste:CG33239, Ste:CG33240, Ste:CG33241, Ste:CG33242, Ste:CG33243, Ste:CG33244, Ste:CG33245, Ste:CG33246 and Ste:CG33247.
          (FlyBase Genome Annotators, 2004)
          hide Comments About Role
          hide Comments About Molecular Function
          The high extent of homology between Ste and Su(Ste) repeats suggested a possibility of Ste suppression by antisense transcription of Su(Ste) elements: however the detection of only "sense" Su(Ste) cDNAs in testis cDNA library argues against this proposal.
          (Kalmykova et al., 1998)
          Extrachromosomal circular DNA (eccDNA) is present throughout the fly's life cycle. The eccDNA population contains circular multimers of tandemly repeated genes, including Ste.
          (Cohen et al., 2003)
          "Stellate-like" sequences (Ste, Su(Ste), SteXh and Ste12DOR) contain a common region of sequence, defined as the "Stellate-specific central core". Specific regions at either the 5' or 3' end of this core sequence distinguish different Stellate-like sequences from each other. Euchromatic Ste sequences all contain at their 5' end a region corresponding to the 3' end of the ben gene.
          (Tritto et al., 2003)
          hide Other Comments
          "1-45.7" was stated as revision.
          (Hardy et al., 1984)
          hide External Crossreferences & Linkouts
          Sequence Crossreferences
          DDBJ / EMBL / GenBank
          •
          UniProtKB/Swiss-Prot
          •
          UniProtKB/TrEMBL
          •
          Other Crossreferences
          InterPro domains - A database of protein families, domains, and functional sites
          •
          Casein kinase II, regulatory subunit (IPR000704)
          Casein kinase II, regulatory subunit, alpha-helical (IPR016149)
          Casein kinase II, regulatory subunit, beta-sheet (IPR016150)
          Linkouts
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          •
          FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
          •
          hide Synonyms & Secondary IDs ( 8 )
          Reported As
          Symbol Synonym
          BcDNA:GM31840
           
          CG32605
          (Bianchi-Frias et al., 2004)
          eSte
          (Rasheva et al., 1997)
          ste
          (Heino, 1994)
          Ste
          (Talbert and Henikoff, 2006, Gvozdev et al., 2005, Cohen et al., 2005)
          Ste eu
          (Tritto et al., 2003)
          Name Synonym
          Stellate
          (Grewal and Elgin, 2007, Olenkina et al., 2007, Pane et al., 2007, Klattenhoff et al., 2007, Zaratiegui, 2007, Gvozdev et al., 2005, Pane et al., 2007, Egorova et al., 2008)
          stellate
          (Klattenhoff et al., 2007, Prasanth and Spector, 2007)
          Secondary FlyBase IDs
          • FBgn0063181
          hide References ( 112 )
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          hide Recent research papers ( 2 )
          Klattenhoff et al., 2007, Dev. Cell 12(1): 45--55
          Drosophila rasiRNA pathway mutations disrupt embryonic axis specification through activation of an ATR/Chk2 DNA damage response. [FBrf0192439]
          Boschi et al., 2006, Genetics 172(1): 305--316
          Genetic evidence that nonhomologous disjunction and meiotic drive are properties of wild-type Drosophila melanogaster male meiosis. [FBrf0190709]
          Show all research papers ...
          hide Recent reviews ( 4 )
          Grewal and Elgin, 2007, Nature 447(7143): 399--406
          Transcription and RNA interference in the formation of heterochromatin. [FBrf0200338]
          Prasanth and Spector, 2007, Genes Dev. 21(1): 11--42
          Eukaryotic regulatory RNAs: an answer to the 'genome complexity' conundrum. [FBrf0193472]
          Zaratiegui, 2007, Cell 128(4): 736--776
          [FBrf0198665]
          Talbert and Henikoff, 2006, Nature Rev. Genet. 7(10): 793--803
          Spreading of silent chromatin: inaction at a distance. [FBrf0194837]
          Show all reviews ...