HomeGene Dmel\spir
| General Information | ||||
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| Symbol | Dmel\spir | Species | D. melanogaster | |
| Name | spire | Annotation symbol | CG10076 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0003475 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 2L | Recombination map | 2-54 | |
| Cytogenetic map | 38C5-38C5 | Sequence location | 2L:20,311,219..20,348,395 [+] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene spire is referred to in FlyBase by the symbol spir (CG10076, FBgn0003475). It has the cytological map location 38C5. Its sequence location is 2L:20311219..20348395. Its molecular function is described as: microtubule binding; actin binding; protein binding; zinc ion binding. It is involved in the biological processes: pole plasm RNA localization; pole plasm assembly; chorion-containing eggshell formation; actin cytoskeleton organization and biogenesis; vesicle-mediated transport; actin filament-based process; actin nucleation; pole plasm oskar mRNA localization; karyosome formation; oogenesis. 42 alleles are reported. The phenotypes of these alleles are annotated with 11 unique terms, many of which group under: anatomical structure; organism; organ system; egg; blastoderm embryo; gamete; embryo; embryonic abdomen; cell part; karyosome. It has 4 annotated transcripts and 4 annotated polypeptides. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | spir: spire (T. Schupbach)
Maternal-effect lethal; homozygous females lay eggs
which sometimes (5-10%) have a "peak" (spire) of dorsal
appendage material sitting over the anterior end of the egg,
instead of two distinct dorsal appendages. Such eggs are
similar to eggs formed by the female-sterile mutation
fs(1)K10, but the extent of dorsal appendage material on spir
eggs is much more variable than that of fs(1)K10 eggs. Mutant
females produce embryos lacking polar granules, pole cells,
and normal abdominal segmentation. In combination with Bic-D,
however, abdominal segmentation does develop in the anterior
half of the embryo; improper localization of abdominal determinants also indicated by the lack of posterior localization
of vasa protein. Cellularization of the blastoderm irregularly defective with nuclei of different sizes and densities.
Resemble embryos formed by other grandchildless-knirps-like
mutations, such as vasa or tudor, but in addition, some of the
embryos from spire females appear also to be dorsalized.
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 38C5-38C5
Limits computationally determined from genome sequence between P{lacW}k09005 and P{EP}CG16798EP401&P{lacW}k07219
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 38C-38C | ||||
| Experimentally Determined Recombination Data | ||||
| Location | 2-54 2-54.5 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | Mapped using spir1. | |||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: spir+ La- spir+ La- rtGEF+ In direction of increasing cytology: anon-38C.32+ spir+ anon-38C.35- Gene Order (overall orientation not stated) References Overall orientation not stated: spir+ La- rtGEF+ Ugt37a1- CG16798- | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\spir
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 4.5, 2.7 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 990, 585 (aa) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
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Sequences Consistent with the Gene Model
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| DDBJ
/
EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation spir[2F] 2L:20,334,150..20,334,150 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=Q246|spir-PD,Q246|spir-PB,Q246@|spir-PA reported_pr_change=Q246@ na_change=C20334150T point mutation spir[1] 2L:20,334,710..20,334,710 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=E292|spir-PD,E292|spir-PB,E292@|spir-PA reported_pr_change=E292@ na_change=G20334710T point mutation spir[4] 2L:20,336,551..20,336,551 comment=Replacement of GGTAA by GGAAA at splice junction causing premature translation termination within intron. evidence=experimental reported_na_change=T?A na_change=T20336551A point mutation spir[PJ56] 2L:20,339,598..20,339,598 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=K415|spir-PD,K416|spir-PB,K416@|spir-PA reported_pr_change=K415@ na_change=A20339598T | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
FlyExpress
- Embryonic expression images (BDGP data)
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele viable (with Df(2L)TW50) viable (with Df(2L)TW65) viable (with Df(2L)pr11163) viable (with Df(2L)pr9201) Sterility Allele Phenotype manifest in Allele | ||||
Stage(s)
1-3
Stage(s)
11-12
Stage(s)
13-16