HomeGene Dmel\smo
| General Information | ||||
|---|---|---|---|---|
| Symbol | Dmel\smo | Species | D. melanogaster | |
| Name | smoothened | Annotation symbol | CG11561 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0003444 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 2L | Recombination map | 2-0.4 | |
| Cytogenetic map | 21B7-21B7 | Sequence location | 2L:277,588..282,167 [+] | |
| Map ( GBrowse ) | 
| |||
Summary Information
| ||||
|
Automatically generated summary
See sections below for more information | The gene smoothened is referred to in FlyBase by the symbol smo (CG11561, FBgn0003444). It has the cytological map location 21B7. Its sequence location is 2L:277588..282167. Its molecular function is described as: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; hedgehog receptor activity; non-G-protein coupled 7TM receptor activity. It is involved in the biological processes: Wnt receptor signaling pathway; blastoderm segmentation; smoothened signaling pathway; eye-antennal disc morphogenesis; anterior/posterior lineage restriction, imaginal disc; Bolwig's organ morphogenesis; signal transduction; regulation of mitotic cell cycle; wing disc anterior/posterior pattern formation; eye morphogenesis. 195 alleles are reported. The phenotypes of these alleles are annotated with 61 unique terms, many of which group under: adult segment; organ system; adult mesothoracic segment; adult; abdominal ventral denticle belt; peripheral nervous system; nervous system; thoracic segment; larval abdominal segment; embryonic nervous system. It has one annotated transcript and one annotated polypeptide. | |||
| External Summaries | ||||
|
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
| ||||
| Gene/Allele symbols may differ from current usage | smo: smoothened
Embryonic lethal. All denticles in abdominal segments point posteriorly. At 18 naked cuticle deleted and denticle belts of adjacent segments fused and locally arranged as
mirror-image duplications.
| |||
|
Detailed Mapping Data
| ||||
| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 21B7-21B7
Limits computationally determined from genome sequence between P{lacW}spenk06805&P{lacW}l(2)k13604k13604 and P{lacW}U2af38k14504
| ||||
| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References | ||||
| Experimentally Determined Recombination Data | ||||
| Location | 2-0.4 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: anon-21Ca? anon-21Cb? smo? In direction of increasing cytology: smo+ U2af38- Hop+ Pi3K21B+ Plc21C+ Gene Order (overall orientation not stated) References | ||||
|
Gene Model & Products
| ||||
Please see the
GBrowse view of
Dmel\smo
for information on other features
| ||||
| Comments on Gene Model | ||||
|
Transcript Data
| ||||
| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 4.1 (northern blot) 4.2 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
|
Polypeptide Data
| ||||
| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
| ||||
| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 1036 (aa) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
| |||
| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Frizzled cysteine-rich domain (IPR000024)
Frizzled protein (IPR000539)
GPCR, family 2, secretin-like (IPR000832)
Frizzled related (IPR015526)
| |||
|
Sequences Consistent with the Gene Model
| ||||
| DDBJ
/
EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
|
Mapped Features & Mutations
| ||||
Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References deletion smo[1K] 2L:279,582..279,592 comment=11bp deletion after codon 437, resulting in a frameshift that causes premature termination after a further 15 codons. evidence=experimental insertion site smo[D16] 2L:278,263..278,263 comment=Insertion of 38 base pairs after codon 124 that introduces two stop codons, one within and the other just downstream of the insertion. evidence=experimental point mutation smo[IA3] 2L:278,160..278,160 comment=Ingham, 2006.06.12, personal communication evidence=experimental na_change=G278160C pr_change=C90S|smo-PA reported_na_change=G?C reported_pr_change=C90S point mutation smo[2] 2L:278,674..278,674 evidence=experimental na_change=G278674A pr_change=C155Y|smo-PA reported_na_change=G1089A reported_pr_change=C155Y point mutation smo[F11] 2L:278,674..278,674 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G278674A pr_change=C155Y|smo-PA reported_pr_change=C155Y point mutation smo[F5]-1 2L:278,674..278,674 comment=One of two amino acid changes observed in smo in mutant allele. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G278674A pr_change=C155Y|smo-PA reported_pr_change=C155Y point mutation smo[F5]-2 2L:278,869..278,869 comment=One of two amino acid changes observed in smo in mutant allele. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G278869A pr_change=S220N|smo-PA reported_pr_change=S220N point mutation smo[1] 2L:279,103..279,103 comment=Also carries eight other nucleotide substitutions, all thought to be conservative changes or polymorphisms. evidence=experimental na_change=G279103A pr_change=C298Y|smo-PA reported_na_change=G1518A reported_pr_change=C298Y point mutation smo[3] 2L:279,368..279,368 comment=TGG to TGA evidence=experimental na_change=G279368A pr_change=W366@|smo-PA reported_na_change=G1783A reported_pr_change=W366@ point mutation smo[3J] 2L:279,433..279,433 comment=Ingham, 2006.06.12, personal communication comment=TGG to TAG evidence=experimental na_change=G279433A pr_change=W388@|smo-PA reported_na_change=G?A reported_pr_change=W388@ point mutation smo[4DI] 2L:279,507..279,508 comment=Not specified if mutation is a change from TGC to TCC or from TGC to AGC. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=C413S|smo-PA reported_pr_change=C413S point mutation smo[2A] 2L:279,690..279,690 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C279690T pr_change=R474C|smo-PA reported_pr_change=R474C rescue fragment smo[+t6.2] 2L:276,621..282,880 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-AatII_rfrag rescue fragment smo[+t6.6] 2L:276,621..283,355 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-HindIII_rfrag | ||||
|
External Data
| ||||
| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | |||
| Crossreferences | ||||
|
Expression Data
| ||||
| FlyBase-Curated Data | ||||
|
Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
|
Summary of Transcript Expression
| ||||
Stage Tissue/Position
Reference
embryonic stage,larval stage,adult stage | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
|
Summary of Polypeptide Expression
| ||||
Stage Tissue/Position
Reference
| ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | plasma membrane | |||
|
External Data & Images
| ||||
| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
| |||
|
Alleles & Phenotypes
| ||||
|
Summary of Allele Phenotypes
| ||||
Lethality Allele Other Phenotypes Allele visible, with Scer\GAL471B Sterility Allele Phenotype manifest in Allele germ cell & embryo | germ-line clone lamina & neuron | precursor | somatic clone photoreceptor cell & neuron adult abdomen & cuticle | anterior compartment adult abdomen & microchaeta | anterior compartment photoreceptor cell R8 & eye disc | somatic clone morphogenetic furrow & nucleus | somatic clone | ||||