• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\sax

General Information
Symbol	Dmel\sax	Species	D. melanogaster
Name	saxophone	Annotation symbol	CG1891
Feature type	protein_coding_gene	FlyBase ID	FBgn0003317
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map	2-57
Cytogenetic map	43E18-43E18	Sequence location	2R:3,693,983..3,697,575 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene saxophone is referred to in FlyBase by the symbol sax (CG1891, FBgn0003317). It has the cytological map location 43E18. Its sequence location is 2R:3693983..3697575. Its molecular function is described as: transforming growth factor beta receptor activity, type I; protein kinase activity; protein serine/threonine kinase activity; ATP binding. It is involved in the biological processes described with 17 unique terms, many of which group under: anatomical structure development; gamete generation; enzyme linked receptor protein signaling pathway; sexual reproduction; transforming growth factor beta receptor signaling pathway; ovarian follicle cell development; regulation of developmental process; transmembrane receptor protein serine/threonine kinase signaling pathway; organ morphogenesis; regionalization. 40 alleles are reported. The phenotypes of these alleles are annotated with 43 unique terms, many of which group under: adult segment; adult mesothoracic segment; organ system; peripheral nervous system; embryonic/larval tracheal system; nervous system; egg; germarium; gastrula embryo; portion of tissue. It has 2 annotated transcripts and 2 annotated polypeptides.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	sax: saxophone (T. Schupbach and E. Wieschaus) Maternal effect lethal, female sterile. Embryos from homozygous mothers gastrulate abnormally. The cephalic furrow is more pronounced than in wildtype. The phenotype is reminiscent of the zygotic embryonic lethal mutaton twisted gastrulaton (twg).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 43E18-43E18   Limits computationally determined from genome sequence between P{EP}CanB2EP774 and P{lacW}lin19k01207&P{lacW}rnh1k07624
Experimentally Determined Cytological Location
Cytogenetic map Notes References 43E-43E (determined by in situ hybridisation)   (Penton et al., 1994) 43E-43E (determined by in situ hybridisation)   (Nellen et al., 1994) 43E-43F (determined by in situ hybridisation)   (Brummel et al., 1994) 43E-43E (determined by in situ hybridisation)   (Xie et al., 1994)
Experimentally Determined Recombination Data
Location	2-57 (Schupbach and Wieschaus, 1989)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\sax for information on other features
Comments on Gene Model
	<new comment>The DGC clone GH13369 represents a rare 5' structure for sax mRNAs. The majority of ESTs have an alternative 5' exon.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) sax-RA FBtr0088832  NM_078928   2424   570 sax-RB FBtr0088833  NM_165559   2719   535
Additional Transcript Data & Comments
Reported size (kB)	3 (northern blot) (Penton et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein sax-PA   FBpp0087908   64.2   570   6.80     NP_523652   AAF59189 sax-PB   FBpp0087909   60.1   535   7.33     NP_724606   AAM68863
Additional Polypeptide Data & Comments
Reported size (kD)	570 (aa) (Brummel et al., 1994, Nellen et al., 1994, Penton et al., 1994) 570 (aa); 62 (kD predicted) (Xie et al., 1994)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003317
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • TGF-beta receptor/activin receptor, type I/II (IPR000472) Protein kinase, core (IPR000719) TGF beta receptor, GS motif (IPR003605) Serine/threonine protein kinase, active site (IPR008271) Protein kinase-like (IPR011009) Protein kinase ATP binding, conserved site (IPR017441) Serine/threonine protein kinase-related (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201752   AA264360   AA390533   AA438725   AA539352   AA817404   AA817656   AA941422   AA951364   AA979340   AE003839 AAF59189   AE003839 AAM68863   AI135563   AI406128   AI454997   AI455432   AI511864   AI517901   AI518717   AI519308   AI531849   AI532181   AI533389   AI533748   AI542414   AW940507   AY058363 AAL13592   BI236884   BI237151   BI242563   BI358624   BI362921   BI363057   BI368722   BI372227   BI481618   BI482899   BI580093   BI637479   BI637993   BP559904   BP559905   BZ286263   CO185065   CO186703   CO263566   CO275555   CO279890   CO287196   CO294370   CO299848   CO304617   CO308533   CO311851   CO311876   CO312427   EC056105   EC059042   EC064976   EC076215   EC198373   EC217729   EC217817   EC231937   EC238229   EC242429   EC245629   EC247329   EC248353   L33476 AAA28878   L33790 AAA61946   LD24180   U05209 AAA18208   U11441 AAA53242   Z70837
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7JPM7 Q7JPN8 Q7JQ39 Q95U21 Q7JQ36
Maps to
Does NOT map to
Identified with	GH13369 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation sax[2] 2R:3,696,323..3,696,323 evidence=experimental pr_change=G412E|sax-PA,G377E|sax-PB comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. reported_pr_change=G412E na_change=G3696323A (Nellen et al., 1994, Brummel et al., 1994, Xie et al., 1994) point mutation sax[1] 2R:3,696,389..3,696,389 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=T434I|sax-PA,T399I|sax-PB reported_pr_change=T434I na_change=C3696389T (Nellen et al., 1994, Brummel et al., 1994, Xie et al., 1994) rescue fragment sax[+t8.9] 2R:3,690,385..3,699,507 linked_to=EcoRI-EcoRI_rfrag comment=Rescues the sterility of sax mutant females and the embryonic pattern defect of their offspring. evidence=experimental (Nellen et al., 1994)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13422
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage-adult stage   (Brummel et al., 1994)  embryonic stage   (Penton et al., 1994)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI135563
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003317 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0003317 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0003317 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for sax Stage(s) 1-3 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | maternal effect saxP lethal | maternal effect | recessive sax1 sax2 lethal | recessive sax1 sax1rv1 sax4 sax5 lethal | embryonic stage | maternal effect sax1 sax2 lethal | embryonic stage | maternal effect | recessive sax1 sax2 lethal | embryonic stage | germ-line clone | non-rescuable maternal effect | recessive sax2R-306-37 sax2R-73-35 lethal | larval stage saxP Other Phenotypes Allele wild-type sax+t8.9 visible saxP visible | recessive | somatic clone sax4 visible | dominant sax4 saxKG07525 visible, with Scer\GAL4ptc-559.1 saxΔI.Scer\UAS visible, with Scer\GAL4en-e16E saxQ263D.Scer\UAS.cDa visible, with Scer\GAL4A9 saxQ263D.Scer\UAS.T:Ivir\HA1 saxΔI.Scer\UAS neurophysiology defective (with sax4) sax5 neurophysiology defective (with sax5) sax4 neuroanatomy defective saxunspecified neuroanatomy defective (with Df(2R)sax-H9) sax4 neuroanatomy defective (with Df(2R)cn7969) sax4 Sterility Allele female sterile sax1 female sterile | recessive sax1 sax2 Phenotype manifest in Allele embryonic/larval dorsal branch, with Scer\GAL4p127 saxΔI.Scer\UAS embryonic/larval dorsal branch, with Scer\GAL4btl.PS saxΔI.Scer\UAS dorsal mesothoracic disc, with Scer\GAL4A9 saxΔI.Scer\UAS wing vein L5, with Scer\GAL4A9 saxΔI.Scer\UAS posterior crossvein, with Scer\GAL4A9 saxΔI.Scer\UAS wing vein L2, with Scer\GAL4A9 saxΔI.Scer\UAS dorsal triple row | proximal, with Scer\GAL4A9 saxΔI.Scer\UAS medial triple row | proximal, with Scer\GAL4A9 saxΔI.Scer\UAS ventral triple row | proximal, with Scer\GAL4A9 saxΔI.Scer\UAS wing, with Scer\GAL4A9 saxΔI.Scer\UAS saxQ263D.Scer\UAS.T:Ivir\HA1 anterior crossvein, with Scer\GAL4ptc-559.1 saxΔI.Scer\UAS ovary saxΔIC.1.hs sax5 egg chamber saxΔIC.1.hs sax1rv5 midgut constriction 2 sax+t8.9 saxP embryonic/first instar larval cuticle | maternal effect sax1 sax2 extended germ band embryo | maternal effect sax1 sax2 amnioproctodeal invagination | maternal effect sax1 sax2 larval epidermis | dorsal | maternal effect sax1 larval midgut & embryo | maternal effect sax1 imaginal disc | maternal effect sax1 extended germ band embryo sax1 sax2 cephalic furrow sax1 sax2 embryonic head | maternal effect sax1 sax2 filzkorper | maternal effect sax1 sax2 abdominal segment 7 | maternal effect sax1 sax2 abdominal segment 8 | maternal effect sax1 sax2 amnioserosa | maternal effect sax1 sax2 saxP ovariole sax1rv5 larval epidermis | dorsal sax2 larval midgut & embryo sax2 imaginal disc sax2 neuromuscular junction (with Df(2R)sax-H9) sax4 bouton (with Df(2R)cn7969) sax4 neuromuscular junction (with Df(2R)cn7969) sax4 ovary | germ-line clone sax4 egg | germ-line clone sax4 sax5 dorsal appendage | germ-line clone sax4 sax5 wing sax4 saxKG07525 wing vein | ectopic sax4 saxKG07525 triple row | ectopic sax4 distal costal vein | ectopic sax4 posterior hair sax4 dorsal double row sax4 ventral double row sax4 wing | somatic clone sax4 wing vein | ectopic | somatic clone sax4 amnioserosa sax4 male germline stem cell | germ-line clone sax4 saxunspecified wing vein | cell non-autonomous | ectopic | somatic clone sax4 dorsal appendage sax5 egg sax5 egg chamber | germ-line clone sax5 filzkorper saxDN.SP6 embryonic head saxDN.SP6 germarium saxP female germline stem cell saxP anterior crossvein saxP eye saxP ommatidium saxP embryonic/larval tracheal system, with Scer\GAL4btl.PS saxQ263D.Scer\UAS wing vein | ectopic, with Scer\GAL4A9 saxQ263D.Scer\UAS.T:Ivir\HA1 wing vein, with Scer\GAL4A9 saxQ263D.Scer\UAS.T:Ivir\HA1 egg chamber, with Scer\GAL4hs.PB saxQ263D.Scer\UAS.cDa wing | posterior, with Scer\GAL4en-e16E saxQ263D.Scer\UAS.cDa wing vein | ectopic, with Scer\GAL4en-e16E saxQ263D.Scer\UAS.cDa bouton saxunspecified synapse saxunspecified t-bar saxunspecified
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of sax Class Mutagen Stocks Known lesion sax1 neomorph, hypomorph ethyl methanesulfonate 0 Yes sax1rv1 amorph 0 -- sax1rv5 ethyl methanesulfonate 0 -- sax2 neomorph, hypomorph ethyl methanesulfonate 0 Yes sax2R-306-37 ethyl methanesulfonate 0 -- sax2R-73-35 ethyl methanesulfonate 0 -- sax3 0 -- sax4 amorph, loss of function ethyl methanesulfonate 1 -- sax5 amorph ethyl methanesulfonate 1 -- sax8 0 -- saxB18 0 -- saxEY04377 0 -- saxKG07525 P-element activity 1 -- saxP amorph, hypomorph P-element activity 0 Yes saxSL306 0 -- saxSL73 0 -- saxhrv1 loss of function 0 --