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Gene Dmel\RpII140

General Information
Symbol	Dmel\RpII140	Species	D. melanogaster
Name	RNA polymerase II 140kD subunit	Annotation symbol	CG3180
Feature type	protein_coding_gene	FlyBase ID	FBgn0003276
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88A9-88A9	Sequence location	3R:9,951,515..9,955,548 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RNA polymerase II 140kD subunit is referred to in FlyBase by the symbol RpII140 (CG3180, FBgn0003276). It has the cytological map location 88A9. Its sequence location is 3R:9951515..9955548. Its molecular function is described as: DNA-directed RNA polymerase activity; DNA binding. It is involved in the biological process transcription from RNA polymerase II promoter. 63 alleles are reported. The phenotypes of these alleles are annotated with: capitellum; cephalopharyngeal skeleton; embryonic abdominal segment; filzkorper; embryonic segment. It has one annotated transcript and one annotated polypeptide.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	RpII140: RNA polymeraseII-140 kd subunit The structural gene for the 140-kd subunit of RNA polymerase II [RNA nucleotidyl transferase (EC 2.7.7.6)]. The gene is highly conserved judging from shared sequence homology with the yeast 150-kd subunit gene.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88A9-88A9   Limits computationally determined from genome sequence between P{PZ}flfl01949 and P{lacW}trxj14A6&P{PZ}trx00347
Experimentally Determined Cytological Location
Cytogenetic map Notes References 88A10-88A10 (determined by in situ hybridisation)   (Ranz et al., 1997) 88A-88B (determined by in situ hybridisation)   (Hamilton, 1990) 88A10-88B2 (determined by in situ hybridisation)   (Hamilton et al., 1993) 88A-88B (determined by in situ hybridisation)   (Parkhurst and Ish-Horowicz, 1991) 88A-88B (determined by in situ hybridisation)   (Falkenburg et al., 1987)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\RpII140 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RpII140-RA FBtr0082892  NM_057358   3773   1176
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein RpII140-PA   FBpp0082353   134.0   1176   7.04     NP_476706   AAF55024
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003276
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA-directed RNA polymerase, subunit 2 (IPR015712) DNA-directed RNA polymerase, subunit 2, domain 6 (IPR007120) RNA polymerase, beta subunit, conserved site (IPR007121) RNA polymerase Rpb2, domain 7 (IPR007641) RNA polymerase Rpb2, domain 2 (IPR007642) RNA polymerase, beta subunit, protrusion (IPR007644) RNA polymerase Rpb2, domain 3 (IPR007645) RNA polymerase Rpb2, domain 4 (IPR007646) RNA polymerase Rpb2, domain 5 (IPR007647)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202158   AA264905   AA390597   AA391748   AA392309   AA438298   AA439595   AA439745   AA440900   AA441251   AA539529   AA567870   AA801692   AA817209   AA941145   AA949469   AA951261   AA951541   AA952152   AC007904   AE003703 AAF55024   AI061750   AI108044   AI113716   AI295348   AI455019   AI455265   AI512737   AI515237   AI515742   AI516406   AI518260   AI518320   AI519108   AI519619   AI520027   AI531621   AI532204   AI532962   AI533230   AI534138   AI534827   AI534857   AI542502   BF504705   BG638945   BI173529   BI232368   BI237933   BI357527   BI361652   BI364901   BI365645   BI372057   BI372928   BI374655   BI375160   BI483800   BI577206   BI579322   BI605708   BI615820   BQ103157   BT003265 AAO25022   CO186347   CO270995   CO280740   CO296706   CO314613   CO318283   CO333971   EC088335   EC089873   EC194642   EC204316   EC208826   EC213913   EC221653   EC225543   EC245236   M62972 AAA28476   M62973   X05709 CAA29180
UniProtKB/Swiss-Prot	P08266
UniProtKB/TrEMBL
Maps to	FBcl0298057 (FlyBase, 1992-)
Does NOT map to
Identified with	LD22387 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation RpII140[S1] 3R:9,953,977..9,953,977 evidence=experimental pr_change=S728C reported_na_change=A2966T|FBrf0103162 reported_pr_change=S728C|FBrf0103162 na_change=A9953977T (Kim et al., 1994) point mutation RpII140[S2] 3R:9,953,977..9,953,977 evidence=experimental pr_change=S728C reported_na_change=A2966T|FBrf0103162 reported_pr_change=S728C|FBrf0103162 na_change=A9953977T (Kim et al., 1994) point mutation RpII140[S8] 3R:9,953,977..9,953,977 pr_change=S728C reported_na_change=A2966T|FBrf0103162 reported_pr_change=S728C|FBrf0103162 evidence=experimental na_change=A9953977T (Kim et al., 1994) point mutation RpII140[S5] 3R:9,953,977..9,953,977 evidence=experimental pr_change=S728C reported_na_change=A2966T|FBrf0103162 reported_pr_change=S728C|FBrf0103162 na_change=A9953977T (Kim et al., 1994) point mutation RpII140[S6] 3R:9,953,977..9,953,977 evidence=experimental pr_change=S728C reported_na_change=A2966T|FBrf0103162 reported_pr_change=S728C|FBrf0103162 na_change=A9953977T (Kim et al., 1994) point mutation RpII140[S11] 3R:9,954,073..9,954,073 evidence=experimental pr_change=M735V reported_na_change=A3063G|FBrf0103162 reported_pr_change=M735V|FBrf0103162 na_change=A9954073G (Kim et al., 1994) point mutation RpII140[S7] 3R:9,954,075..9,954,075 evidence=experimental pr_change=M735I reported_na_change=G3065A|FBrf0103162 reported_pr_change=M735I|FBrf0103162 na_change=G9954075A (Kim et al., 1994) point mutation RpII140[S12] 3R:9,954,075..9,954,075 evidence=experimental pr_change=M735I reported_na_change=G3065A|FBrf0103162 reported_pr_change=M735I|FBrf0103162 na_change=G9954075A (Kim et al., 1994) point mutation RpII140[Z43] 3R:9,954,689..9,954,689 evidence=experimental pr_change=R940H reported_na_change=G3678A|FBrf0059056 reported_pr_change=R940H|FBrf0059056 na_change=G9954689A (Chen et al., 1993) point mutation RpII140[M39] 3R:9,954,845..9,954,845 evidence=experimental pr_change=G992E reported_na_change=G3834A|FBrf0059056 reported_pr_change=G992E|FBrf0059056 na_change=G9954845A (Chen et al., 1993) point mutation RpII140[Z19] 3R:9,954,952..9,954,952 pr_change=E1028K evidence=experimental reported_na_change=G3941A|FBrf0059056 reported_pr_change=E1028K|FBrf0059056 na_change=G9954952A (Chen et al., 1993) point mutation RpII140[Z45] 3R:9,955,222..9,955,222 evidence=experimental pr_change=S1098F reported_na_change=C4211T|FBrf0059056 reported_pr_change=S1098F|FBrf0059056 na_change=C9955222T (Chen et al., 1993) sequence variant RpII140[A5] 3R:9,955,069..9,955,083 comment=deletion evidence=experimental (Chen et al., 1993)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 9373
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI519619
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003276 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0003276 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0003276 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for RpII140 Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable RpII140S1 RpII140S2 lethal | recessive RpII140A5 RpII140M16 RpII140M21 RpII140M25 RpII140M28 RpII140M37 RpII140M38 RpII140M39 RpII140M3 RpII140M44 RpII140M46 RpII140M4 RpII140M50 RpII140M5 RpII140M6 RpII140M8 RpII140S1R1 RpII140S1R2 RpII140S1R3 RpII140S1R4 RpII140S1R5 RpII140S1R6 RpII140S2R RpII140S8 RpII140S8R RpII140Z19 RpII140Z20 RpII140Z22 RpII140Z24 RpII140Z31 RpII140Z34 RpII140Z39 RpII140Z3 RpII140Z45 RpII140Z50 RpII140Z51 RpII140Z5 RpII140Z7 RpII140brie lethal | recessive | conditional - heat sensitive RpII140Z36 RpII140Z43 lethal | conditional - heat sensitive RpII140Z36 lethal | embryonic stage | recessive RpII140wimp lethal | embryonic stage | germ-line clone | maternal effect | recessive RpII1403R-112-6 RpII1403R-92-38 Other Phenotypes Allele wild-type RpII140+tcos15 RpII140+tcos92 RpII140-IN Phenotype manifest in Allele cephalopharyngeal skeleton | germ-line clone | maternal effect RpII1403R-112-6 RpII1403R-92-38 embryonic abdominal segment | germ-line clone | maternal effect RpII1403R-112-6 RpII1403R-92-38 filzkorper | germ-line clone | maternal effect RpII1403R-112-6 RpII1403R-92-38 embryonic segment | germ-line clone | maternal effect RpII1403R-112-6 RpII1403R-92-38 capitellum RpII140M39 RpII140Z43 RpII140Z45
Classical Alleles ( 54 )
For All Classical Alleles Show
Allele of RpII140 Class Mutagen Stocks Known lesion RpII1403R-112-6 ethyl methanesulfonate 0 -- RpII1403R-92-38 ethyl methanesulfonate 0 -- RpII140A5 γ ray 0 Yes RpII140AS 0 -- RpII140M16 ethyl methanesulfonate 0 -- RpII140M21 ethyl methanesulfonate 0 -- RpII140M25 ethyl methanesulfonate 0 -- RpII140M28 ethyl methanesulfonate 0 -- RpII140M37 ethyl methanesulfonate 0 -- RpII140M38 ethyl methanesulfonate 0 -- RpII140M39 ethyl methanesulfonate 0 Yes RpII140M3 ethyl methanesulfonate 0 -- RpII140M44 ethyl methanesulfonate 0 -- RpII140M46 ethyl methanesulfonate 0 -- RpII140M4 ethyl methanesulfonate 0 -- RpII140M50 ethyl methanesulfonate 0 -- RpII140M5 ethyl methanesulfonate 0 -- RpII140M6 ethyl methanesulfonate 0 -- RpII140M8 ethyl methanesulfonate 0 -- RpII140S11 ethyl methanesulfonate 0 -- RpII140S12 ethyl methanesulfonate 0 Yes RpII140S1 ethyl methanesulfonate 0 Yes RpII140S1R1 ethyl methanesulfonate 0 -- RpII140S1R2 ethyl methanesulfonate 0 -- RpII140S1R3 ethyl methanesulfonate 0 -- RpII140S1R4 ethyl methanesulfonate 0 -- RpII140S1R5 ethyl methanesulfonate 0 -- RpII140S1R6 ethyl methanesulfonate 0 -- RpII140S2 ethyl methanesulfonate 0 Yes RpII140S2R ethyl methanesulfonate 0 -- RpII140S5 ethyl methanesulfonate 0 Yes RpII140S6 ethyl methanesulfonate 0 Yes RpII140S7 ethyl methanesulfonate 0 Yes RpII140S8 ethyl methanesulfonate 0 Yes RpII140S8R 0 -- RpII140Z19 ethyl methanesulfonate 0 Yes RpII140Z20 ethyl methanesulfonate 0 -- RpII140Z22 ethyl methanesulfonate 0 -- RpII140Z24 ethyl methanesulfonate 0 -- RpII140Z31 ethyl methanesulfonate 0 -- RpII140Z34 ethyl methanesulfonate 0 -- RpII140Z36 ethyl methanesulfonate 0 -- RpII140Z39 ethyl methanesulfonate 0 -- RpII140Z3 ethyl methanesulfonate 0 -- RpII140Z43 ethyl methanesulfonate 0 Yes RpII140Z45 ethyl methanesulfonate 0 Yes RpII140Z50 ethyl methanesulfonate 0 -- RpII140Z51 ethyl methanesulfonate 0 -- RpII140Z5 ethyl methanesulfonate 0 -- RpII140Z7 ethyl methanesulfonate 0 -- RpII140brie ethyl methanesulfonate 0 -- RpII140unspecified 0 -- RpII140wimp 2 -- RpII140wimpRQ γ ray 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RpII140 Class Mutagen Stocks Known lesion RpII140+tcos15 in vitro construct | genomic fragment 0 Yes RpII140+tcos92 in vitro construct | genomic fragment 0 Yes RpII140-IN in vitro construct | deletion 0 Yes RpII140DNAP in vitro construct | amino acid replacement 0 Yes RpII140GD4233 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes RpII140M in vitro construct | amino acid replacement in vitro construct | site directed mutagenesis 0 Yes RpII140S in vitro construct | amino acid replacement in vitro construct | site directed mutagenesis 0 Yes RpII140SM in vitro construct | site directed mutagenesis 0 Yes RpII140cos50 in vitro construct | genomic fragment 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)su(Hw)7
Disrupted in	Df(3R)Exel7321 (Ryder, 2004.4.26) Df(3R)Exel7359 (Ryder, 2004.4.26) Df(3R)JY19 (Breen and Harte, 1991) Df(3R)red-P1 (Jarman and Collett, 1998, Mortin et al., 1992)