HomeGene Dmel\Ras85D
| General Information | ||||
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| Symbol | Dmel\Ras85D | Species | D. melanogaster | |
| Name | Ras oncogene at 85D | Annotation symbol | CG9375 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0003205 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 3R | Recombination map | 3-50 | |
| Cytogenetic map | 85D19-85D19 | Sequence location | 3R:5,336,273..5,338,786 [-] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene Ras oncogene at 85D is referred to in FlyBase by the symbol Ras85D (CG9375, FBgn0003205). It has the cytological map location 85D19. Its sequence location is 3R:5336273..5338786. Its molecular function is described as: GTPase activity; GDP binding; GTP binding. It is involved in the biological processes described with 36 unique terms, many of which group under: anatomical structure development; regulation of developmental process; regulation of biological process; organ morphogenesis; ovarian follicle cell development; regulation of apoptosis; gamete generation; sensory organ development; cell motility; organ development. 110 alleles are reported. The phenotypes of these alleles are annotated with 124 unique terms, many of which group under: peripheral nervous system; organ system; nervous system; adult segment; embryonic nervous system; imaginal precursor; adult mesothoracic segment; thoracic segment; embryonic/larval tracheal system; embryonic neuron; adult. It has one annotated transcript and one annotated polypeptide. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | Ras1: Ras proto-oncogene sequence
Codes for a polypeptide of 21.6 kilodaltons; residues 1-121 and 137-64 exhibit 75% homology with vertebrate H-ras protein.
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 85D19-85D19
Limits computationally determined from genome sequence between P{PZ}ps10615&P{PZ}Ras85D06677 and P{EP}CG9393EP3122
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 85D18-85D20 (determined by in situ hybridisation)
85D10-85D10 (determined by in situ hybridisation)
85D1-85D8 85D18--20
85D-85D (determined by in situ hybridisation)
85D-85D (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | 3-50 | |||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References Overall orientation not stated: Rlc1+ Rlb1- Ras85D+ | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\Ras85D
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 1.3 (northern blot) 2.0, 1.3 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | ||||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Ras GTPase (IPR001806)
Ras small GTPase, Ras type (IPR003577)
Small GTP-binding protein (IPR005225)
Ras (IPR013753)
Ras small GTPase, Ras-related (IPR015592)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation Ras85D[e2F] 3R:5,337,902..5,337,902 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G5337902A pr_change=D38N|Ras85D-PA reported_pr_change=D38N point mutation Ras85D[Sutor-404] 3R:5,337,874..5,337,874 comment=Mutation in splice donor sequence which changes the conserved GU to AU. evidence=experimental na_change=G5337874A reported_na_change=G?A point mutation Ras85D[2F4] 3R:5,337,683..5,337,683 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G5337683A pr_change=A59T|Ras85D-PA reported_pr_change=A59T point mutation Ras85D[e1B] 3R:5,337,674..5,337,674 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G5337674A pr_change=E62K|Ras85D-PA reported_pr_change=E62K point mutation Ras85D[bE382] 3R:5,337,671..5,337,671 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G5337671A pr_change=E63K|Ras85D-PA reported_pr_change=E63K point mutation Ras85D[Sutor-341] 3R:5,337,467..5,337,467 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=A5337467T pr_change=R131@|Ras85D-PA reported_pr_change=R131@ rescue fragment Ras85D[+t12] 3R:5,332,231..5,343,826 rescue fragment Ras85D[+t5.5] 3R:5,336,635..5,342,154 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=EcoRI-EcoRI_rfrag rescue fragment Ras85D[+t3.6] 3R:5,336,377..5,339,850 comment=Position of restriction fragment on reference sequence inferred by FlyBase curator. evidence=experimental linked_to=HindIII-HindIII_rfrag | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
embryonic stage
oogenesis stage | unfertilized egg
embryonic stage | early
adult stage
abdominal ganglion
adult stage
thoracic ganglion
oogenesis stage,adult stage
oocyte
larval stage | third instar
dorsal mesothoracic disc
larval stage | third instar
eye-antennal disc
larval stage | third instar
embryonic/larval brain | restricted
adult stage
adult brain | resticted
larval stage | third instar
genital disc | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
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| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele Other Phenotypes Allele visible, with Scer\GAL4dpp.blk1 | ||||
