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Gene Dmel\px

General Information
Symbol	Dmel\px	Species	D. melanogaster
Name	plexus	Annotation symbol	CG4444
Feature type	protein_coding_gene	FlyBase ID	FBgn0003175
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2R	Recombination map	2-100.5
Cytogenetic map	58E4-58E8	Sequence location	2R:18,384,003..18,449,569 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene plexus is referred to in FlyBase by the symbol px (CG4444, FBgn0003175). It has the cytological map location 58E4-58E8. Its sequence location is 2R:18384003..18449569. Its molecular function is unknown. It is involved in the biological process imaginal disc-derived wing vein morphogenesis. 44 alleles are reported. The phenotypes of these alleles are annotated with: wing vein; wing; eye; leg. It has 3 annotated transcripts and 3 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	px: plexus px: plexus From "Edith M. Wallace, unpublished." Wings have network of extra veins, especially towards tips and margins; L4 vein bent near tip. Semidominant with some Minutes. Suppressed by S (Bedichek, 1936, DIS 4: 24); suppresses expression of ve (Thompson, 1973, DIS 50: 59). Venation effect caused by inadequate contraction of wing during pupal stage, leaving spaces between epithelial layers (Waddington, 1940). Vein patterns of px flies studied in lines selected for increased or decreased expression (Thompson, 1974).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 58E4-58E8   Limits computationally determined from genome sequence between P{lacW}l(2)k13211k13211&P{EP}EP827 and P{PZ}l(2)rG270rG270
Experimentally Determined Cytological Location
Cytogenetic map Notes References 58E1-58E2 (determined by in situ hybridisation) 59B1--2 (determined by in situ hybridisation)   (Spradling, 1999.12.9) 58E1-58E2 (determined by in situ hybridisation)   (Spradling et al., 1999) 58E1-58E2 59B1--2   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-100.5 (Williams, 1956)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: px? robo? (Tear, 1997.6.13) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\px for information on other features
Comments on Gene Model
	DGC clone appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) px-RA FBtr0071838  NM_166535   6847   1995 px-RB FBtr0112820  NM_001103947   5034   1535 px-RC FBtr0112821  NM_001103948   1358   244
Additional Transcript Data & Comments
Reported size (kB)	7.3, 5.8, 5.3 (northern blot) (Matakatsu et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein px-PA   FBpp0071749   213.9   1995   8.77     NP_726208   AAF46855 px-PB   FBpp0111732   164.1   1535   8.94     NP_001097417   ABV53884 px-PC   FBpp0111733   26.4   244   9.35     NP_001097418   ABV53885
Additional Polypeptide Data & Comments
Reported size (kD)	1990 (aa); 213 (kD predicted) (Matakatsu et al., 1999)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0003175
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390944   AA699237   AB032181 BAA86717   AE003457 AAF46855   AI530850   AI531115   AI541785   AI542354   AQ025858   AQ034014   AW944190   AY058753 AAL13982   BG637998   BI367551   BI367593   BI575864   BI584433   BI633245   BI636560   BI636624   BT029592   CK445224   CL527722   CL528203   CL844327   CO190198   CO270436   CO274099   CO278895   CO286158   CO296848   CO299982   CO302855   CO303553   CO303863   CO304967   CO315485   CO325929   CO329021   CO331803   CO337451   CO339269   CO344394   CZ467475   CZ469640   CZ469704   CZ469705   CZ470157   CZ470650   CZ470651   CZ470670   CZ471622   CZ472532   CZ472533   CZ474591   CZ480715   CZ482884   CZ482885   CZ484654   CZ485850   CZ487328   CZ489242   EC053488   EC053626   EC053740   EC054343   EC055476   EC056589   EC060885   EC060986   EC064949   EC074475   EC077126   EC083484   EC084169   EC088507   EC195512   EC195955   EC199249   EC205206   EC208405   EC234256   EC238385   EC241395   EC247877   EC254790   EC255568   EC256710   EC257465   EC264296   EL885465   EL885696   EL885697   EL885817   LD11785
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q95TI5 Q9U8Q0 Q9W244
Maps to
Does NOT map to
Identified with	SD01586 (BDGP Project Members, 2000-) Dm4330 (Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References aberration junction In(2LR)px[52g] 2R:18,419,681..18,419,749 comment=The inversion breakpoint maps to a XhoI-ApaI restriction fragment. evidence=experimental linked_to=XhoI-ApaI_rfrag (Matakatsu et al., 1999) point mutation px[72] 2R:18,443,274..18,443,274 evidence=experimental na_change=C18063351T pr_change=Q339@|px-PA reported_pr_change=Q339@ comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Matakatsu et al., 1999)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 1327
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage,larval stage   (Matakatsu et al., 1999)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  larval stage | third instar  dorsal mesothoracic disc (Matakatsu et al., 1999)
Marker for
Subcellular Localization
CV Term	nucleus (Matakatsu et al., 1999)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003175 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0003175 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0003175
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable pxEY03862 pxEY10083 pxEY10541 pxT190-202 pxe02948 lethal | recessive pxk08134 pxk08316 Other Phenotypes Allele wild-type pxhs.PM visible px1 px2 px52g px54h px55k pxe02948 pxhb246 visible | recessive px1 px72 visible | recessive | somatic clone px72 visible, with Scer\GAL4C-765 pxScer\UAS.cMa Sterility Allele fertile pxEY03862 pxEY10083 pxEY10541 pxe02948 Phenotype manifest in Allele wing vein px1 px72 pxM1 pxM2 pxM3 pxM4 pxM5 pxM6 pxe02948 wing px1 px2 px52g px54h px55k px72 pxM1 pxM2 pxM3 pxM4 pxM5 pxM6 pxhb246 wing vein | ectopic px1 px72 pxhb246 eye px1 leg px1 wing vein | ectopic | somatic clone px72 wing, with Scer\GAL4C-765 pxScer\UAS.cMa wing, with Scer\GAL4ptc-559.1 pxScer\UAS.cMa
Classical Alleles ( 41 )
For All Classical Alleles Show
Allele of px Class Mutagen Stocks Known lesion px19 0 -- px1 spontaneous 85 -- px2 spontaneous 0 -- px45j natural population 0 -- px52g X ray 0 Yes px54h spontaneous 0 -- px55k spontaneous 0 -- px70 spontaneous 0 -- px72 loss of function, hypomorph 0 Yes pxA130.1F2 P-element activity 1 Yes pxDG29811 P-element activity 1 -- pxEY03862 P-element activity 1 -- pxEY10083 P-element activity 1 -- pxEY10541 P-element activity 0 -- pxM1 0 -- pxM2 0 -- pxM3 0 -- pxM4 0 -- pxM5 0 -- pxM6 0 -- pxMB02577 1 -- pxT190-202 P-element activity 0 Yes pxc01821 piggyBac transposase 0 -- pxc04958 piggyBac transposase 1 -- pxc05044 piggyBac transposase 0 -- pxc05653 piggyBac transposase 0 -- pxc06385 piggyBac transposase 1 -- pxc06408 piggyBac transposase 1 -- pxd00474 P-element activity 1 -- pxd01959 P-element activity 1 -- pxd05539 P-element activity 0 -- pxe02948 piggyBac transposase 2 -- pxf00418 piggyBac transposase 1 -- pxf02152 piggyBac transposase 0 -- pxf03306 piggyBac transposase 1 -- pxf04768 piggyBac transposase 1 -- pxf06534 piggyBac transposase 1 -- pxhb246 hobo activity 0 Yes pxk08134 P-element activity 0 -- pxk08316 P-element activity 1 -- pxunspecified 0 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of px Class Mutagen Stocks Known lesion pxGD11318 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes pxScer\UAS.cMa in vitro construct | regulatory fusion 0 Yes pxhs.PM in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)X58-9
Useful duplication	Dp(2;2)l-24
Duplicated in	Dp(2;3)P
Not disrupted in	Df(2R)02311 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)59AB (BDGP Project Members, 1994-1999) Df(2R)59AD (BDGP Project Members, 1994-1999) Df(2R)E3578 (BDGP Project Members, 1994-1999) Df(2R)X58-7 (BDGP Project Members, 1994-1999)
Disrupted in	Df(2R)M58F (Bridges, 1937, Dunn and Mossige, 1937) Df(2R)P (Bridges, 1937) Df(2R)X58-6 (Kerrebrock et al., 1992) In(2LR)px52g (Matakatsu et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11318} NA P{UAS-px.M} NA heat-shock construct P{hs-px.M} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions H{}pxhb246 NA PBac{PB}pxc01821 NA PBac{PB}pxc04958 NA PBac{PB}pxc05044 NA PBac{PB}pxc05653 NA PBac{PB}pxc06385 NA PBac{PB}pxc06408 NA PBac{RB}pxe02948 NA PBac{WH}pxf00418 NA PBac{WH}pxf02152 NA PBac{WH}pxf03306 NA PBac{WH}pxf04768 NA PBac{WH}pxf06534 NA P{T1}pxT190-202 NA