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Gene Dmel\Acp70A

General Information
Symbol	Dmel\Acp70A	Species	D. melanogaster
Name	Accessory gland peptide 70A	Annotation symbol	CG17673
Feature type	protein_coding_gene	FlyBase ID	FBgn0003034
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	70A4-70A4	Sequence location	3L:13,294,735..13,295,022 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Accessory gland peptide 70A is referred to in FlyBase by the symbol Acp70A (CG17673, FBgn0003034). It has the cytological map location 70A4. Its sequence location is 3L:13294735..13295022. Its molecular function is described as hormone activity. It is involved in the biological processes described with 11 unique terms, many of which group under: reproductive process in a multicellular organism; regulation of response to stimulus; mating; regulation of female receptivity; female receptivity; terpenoid metabolic process; regulation of biological process; oviposition; adult feeding behavior; behavioral interaction between organisms; gamete generation. 26 alleles are reported. The phenotype of these alleles is annotated with spermatozoon. It has one annotated transcript and one annotated polypeptide.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Acp70A: Accessory gland peptide Encodes a 36-amino-acid peptide that is synthesized in the accessory gland and is transferred to the female where it represses female sexual receptivity and stimulates oviposition. The peptide contains a high concentration of basic amino acids, tryptophan and hydroxyproline as well as an unique residue of unknown nature that is encoded by a leucine codon.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 70A4-70A4   Limits computationally determined from genome sequence between P{PZ}l(3)0422004220&P{lacW}l(3)j10B6j10B6 and P{PZ}stv00543
Experimentally Determined Cytological Location
Cytogenetic map Notes References 70A-70A (determined by in situ hybridisation)   (Segarra et al., 1996) 70A-70A (determined by in situ hybridisation)   (Chen et al., 1988)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Acp70A for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Acp70A-RA FBtr0075836  NM_079333   223   55
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Acp70A-PA   FBpp0075572   6.4   55   10.78     NP_524057   AAF49836
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sex peptide (IPR012608)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003538 AAF49836   AY344285 AAR04600   AY344286 AAR04601   AY344287 AAR04602   AY344288 AAR04603   AY344289 AAR04604   AY344290 AAR04605   AY344291 AAR04606   AY344292 AAR04607   AY344293 AAR04608   AY344294 AAR04609   AY344295 AAR04610   AY344296 AAR04611   AY344297 AAR04612   AY344298 AAR04613   AY344299 AAR04614   AY344300 AAR04615   AY344301 AAR04616   AY344302 AAR04617   AY344303 AAR04618   AY344304 AAR04619   EL869980   EL869981   EL869982   EL869983   M21201 AAA28816   X99407 CAA67784   X99408 CAA67785   X99409 CAA67786   X99410 CAA67787   X99411 CAA67788   X99413 CAA67790   X99415 CAA67792   X99416 CAA67793   X99418 CAA67795
UniProtKB/Swiss-Prot	P05623
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with	D1177 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References rescue fragment Acp70A[+t2] 3L:13,293,869..13,295,774 comment=Females mated with males carrying one copy of the rescue construct show normal postmating responses; position of rescue fragment on reference sequence inferred by FlyBase curator evidence=experimental (Peng et al., 2005)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 4205
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0003034 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0003034 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0003034
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4αTub84B.PL Acp70AScer\UAS.cKa Other Phenotypes Allele behavior defective | female (with Df(3L)Δ130) Acp70A0 courtship defective | female Acp70Ag.Yp1.hs courtship defective | female (with Df(3L)Δ130) Acp70A0 feeding behavior defective | female limited Acp70A0 Acp70Ag.Yp1.hs feeding behavior defective | female limited, with Scer\GAL4C370 Acp70AScer\UAS.cNa feeding behavior defective | female limited, with Scer\GAL49Y Acp70AScer\UAS.cNa Phenotype manifest in Allele spermatozoon, with Acp70A0 Acp70AΔ2-7 Acp70AQQ spermatozoon Acp70A0 spermatozoon, with Acp70AQQ Acp70A0 spermatozoon, with Acp70AΔ2-7 Acp70A0
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Acp70A Class Mutagen Stocks Known lesion Acp70A+.3' recombination SCEI endonuclease FLPase 0 Yes Acp70A0.5' recombination FLPase SCEI endonuclease 0 Yes Acp70A0 I-CreI endonuclease 0 Yes
Alleles Carried on Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Acp70A Class Mutagen Stocks Known lesion Acp70A+t2 in vitro construct | genomic fragment 0 Yes Acp70A21-36 in vitro construct | other 0 Yes Acp70A25-36 in vitro construct | other 0 Yes Acp70A6x.T:Zzzz\His6 in vitro construct | coding region fusion 0 Yes Acp70A8-20 in vitro construct | other 0 Yes Acp70A8-23 in vitro construct | other 0 Yes Acp70A8-36 in vitro construct | other 0 Yes Acp70AGD3878 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Acp70AQQ in vitro construct | site directed mutagenesis 0 Yes Acp70AScer\SceI.RS.cLa in vitro construct | other 0 Yes Acp70AScer\UAS.6x.T:Zzzz\His6 in vitro construct | coding region fusion 0 Yes Acp70AScer\UAS.T:Hsap\GGT1 in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes Acp70AScer\UAS.cKa in vitro construct | regulatory fusion 0 Yes Acp70AScer\UAS.cNa in vitro construct | regulatory fusion 0 Yes Acp70Ac.Yp1.hs in vitro construct | regulatory fusion 0 Yes Acp70AcSa in vitro construct | other 0 Yes Acp70Acm1-34 in vitro construct | other 0 Yes Acp70AdsRNA.Scer\UAS in vitro construct | regulatory fusion in vitro construct | RNAi 0 Yes Acp70Afl.cSa in vitro construct | other 0 Yes Acp70Ag.Yp1.hs in vitro construct | other 1 Yes Acp70Ahs.PA in vitro construct | regulatory fusion 0 Yes Acp70Ahs.T:Hsap\GGT1 in vitro construct | coding region fusion in vitro construct | regulatory fusion 0 Yes Acp70AΔ2-7 in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3L)VW1
Useful duplication	Dp(3;3)M71
Disrupted in	Df(3L)Exel6118 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3878} NA P{UAS-Acp70A.6His} NA P{UAS-Acp70A.GGT} NA P{UAS-Acp70A.IR} NA P{UAS-Acp70A.K} NA P{UAS-Acp70A.N} NA characterization construct P{Acp70A.TGQQ} NA P{Acp70A.TGWT} NA P{Acp70A.TGΔ2-7} NA P{FRT(w+.Acp70AScer\SceI.RS.cLa)} NA heat-shock construct P{Acp70Ac.Yp1.hs} NA P{Acp70Ag.Yp1.hs} NA P{Acp70Ahs.PA} NA P{hs-GGT-SP} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data Once transfered to the female Acp70A represses female sexual receptivity and stimulates oviposition. (Lindsley and Zimm, 1992) Injection of Acp70A or ectopic expression of Acp70A in virgin females causes reduced receptivity and increased ovulation and oviposition. These behavioural changes suggest that the nervous system is involved in females' reaction to Acp70A. (Fleischmann et al., 1995) In a screen of 204 randomly selected Scer\GAL4 enhancer trap lines 33 give rise to Sex Peptide responses, but none separate the behavioural and physiological responses. These results indicate that the two responses are either identical, very close together or in two anatomically distinct tissues expression a common set of genes. (Nakayama et al., 1997)
Gene Ontology: Function, Process & Cellular Component ( 13 )
Molecular Function
CV term Evidence References
hormone activity non-traceable author statement (Hewes and Taghert, 2001) non-traceable author statement (FlyBase, 1992-) inferred from electronic annotation with InterPro:IPR012608 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
adult feeding behavior inferred from mutant phenotype (Carvalho et al., 2006)
juvenile hormone biosynthetic process non-traceable author statement (Fan et al., 1999)
negative regulation of female receptivity traceable author statement (Kubli, 2003) non-traceable author statement (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1988.11.1)
negative regulation of female receptivity, post-mating traceable author statement (Fan et al., 1999) traceable author statement (Greenspan and Ferveur, 2000) inferred from mutant phenotype (Liu and Kubli, 2003) inferred from mutant phenotype (Chapman et al., 2003) traceable author statement (Chapman, 2001) traceable author statement (Billeter et al., 2002) traceable author statement (Wolfner, 2002)
oogenesis traceable author statement (Kubli, 2003) traceable author statement (Chapman, 2001) traceable author statement (Wolfner, 2002)
oviposition traceable author statement (Greenspan and Ferveur, 2000) non-traceable author statement (Swiss-Prot Project Members, 1988.11.1)
post-mating behavior traceable author statement (Greenspan and Ferveur, 2000)
regulation of female receptivity, post-mating inferred from mutant phenotype (Peng et al., 2005)
regulation of juvenile hormone biosynthetic process traceable author statement (Kubli, 2003)
regulation of oviposition traceable author statement (Kubli, 2003) traceable author statement (Billeter et al., 2002)
regulation of oviposition, post-mating inferred from mutant phenotype (Liu and Kubli, 2003) inferred from mutant phenotype (Peng et al., 2005) traceable author statement (Chapman, 2001) traceable author statement (Wolfner, 2002)
Cellular Component
CV term Evidence References
extracellular region non-traceable author statement (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1988.11.1) traceable author statement (Chapman, 2001) inferred from electronic annotation with InterPro:IPR012608 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Acp70A allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 64839
Orthologs
Genome-wide drosophilid orthologs	Dere\GG15632 Dsec\Acp70A Dsim\Acp70A Dyak\GE21959
Curated drosophilid orthologs	Dgua\Acp70A   Dmad\Acp70Aa (Cirera, 1998.3.13) Dmad\Acp70Ab (Cirera, 1998.3.13) Dmau\Acp70A   Dmir\Acp70A   Dper\Acp70A   Dpsb\Acp70A (Schully and Hellberg, 2006.1.12) Dpse\Acp70A   Dsec\Acp70A   Dsim\Acp70A   Dsub\Acp70Aa (Cirera, 1998.3.5) Dsub\Acp70Ab (Cirera, 1998.3.5) Dsuz\SP
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	4365 P{Acp70Ag.Yp1.hs}G10
VDRC	v52425 w1118; P{GD3878}v52425
Genomic Clones ( 1 )
	BACR15O08
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	IP02110 IP02112 IP02109
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0003034 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0003034
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
	Biochemical analysis and molecular regulation studies of the male accessory gland secretions of Acp70A demonstrate that Acp70A gene product inhibits remating and enhances the ovulation of virgin females. Acp70A gene product has no effect on virgins of phylogenetically related species. (Chen, 1991) The Acp70A gene product elicits the post-mating behaviour in females. (Aigaki et al., 1991) Acp70A injection mimics the effects of mating, suggesting Acp70A is the major effector in the changes induced by mating with respect to increase egg production and laying. (Soller et al., 1997)
Comments About Molecular Function
	Encodes a 36-amino-acid peptide that is synthesized in the accessory gland and is transferred to the female. The peptide contains a high concentration of basic amino acids, tryptophan and hydroxyproline as well as an unique residue of unknown nature that is encoded by a leucine codon. (Lindsley and Zimm, 1992)
Other Comments
	The D.sechellia Acp70A gene product shows high identity to that of D.melanogaster. (Chen and Balmer, 1989) Both purified Acp70A from D.suzukii and synthetic D.melanogaster Acp70A reduce receptivity and induce ovulation in D.suzukii and D.melanogaster females, confirming functional homology. (Schmidt et al., 1993) Chromosome homologies of Muller's element D (J chromosome in the Paleartic species and XR chromosome arm in Nearctic species) and of element E (O chromosome in the Paleartic species and 2 chromosome in Nearctic species) have been confirmed by single copy probes in the species of the obscura group and in D.melanogaster. (Segarra et al., 1996) Females are capable of repelling males be emitting a volatile pheromone(s) with an inhibitory effect on male courtship. (Fuyama and Ueyama, 1997) Application of methoprene mimics the Acp70A product-mediated stimulation of vitellogenic oocyte progression in sexually mature virgin females. (Soller et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079333 RefSeq (Proteins) • NP_524057 DDBJ / EMBL / GenBank • AE003538 AAF49836 AY344285 AAR04600 AY344286 AAR04601 AY344287 AAR04602 AY344288 AAR04603 AY344289 AAR04604 AY344290 AAR04605 AY344291 AAR04606 AY344292 AAR04607 AY344293 AAR04608 AY344294 AAR04609 AY344295 AAR04610 AY344296 AAR04611 AY344297 AAR04612 AY344298 AAR04613 AY344299 AAR04614 AY344300 AAR04615 AY344301 AAR04616 AY344302 AAR04617 AY344303 AAR04618 AY344304 AAR04619 M21201 AAA28816 X99407 CAA67784 X99408 CAA67785 X99409 CAA67786 X99410 CAA67787 X99411 CAA67788 X99413 CAA67790 X99415 CAA67792 X99416 CAA67793 X99418 CAA67795 UniProtKB/Swiss-Prot • P05623
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sex peptide (IPR012608)