HomeGene Dmel\osk
| General Information | ||||
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| Symbol | Dmel\osk | Species | D. melanogaster | |
| Name | oskar | Annotation symbol | CG10901 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0003015 | |
| Created / Updated | 2005-01-10/2005-01-10 | |||
| Genomic Location | ||||
| Chromosome (arm) | 3R | Recombination map | 3-48.4 | |
| Cytogenetic map | 85B7-85B7 | Sequence location | 3R:4,760,853..4,764,123 [+] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene oskar is referred to in FlyBase by the symbol osk (CG10901, FBgn0003015). It has the cytological map location 85B7. Its sequence location is 3R:4760853..4764123. Its molecular function is unknown. It is involved in the biological processes described with 17 unique terms, many of which group under: anterior/posterior axis specification; oocyte axis determination; gamete generation; oocyte anterior/posterior axis determination; pole cell development; regulation of metabolic process; pole plasm assembly; cellular macromolecule metabolic process; tripartite regional subdivision; mRNA metabolic process; posterior abdomen determination; positive regulation of cellular biosynthetic process; memory. 102 alleles are reported. The phenotypes of these alleles are annotated with 33 unique terms, many of which group under: embryonic abdomen; organ system; embryonic segment; anatomical structure; embryonic tagma; cephalopharyngeal skeleton; embryonic head; extended germ band embryo; thoracic segment; prothoracic segment. It has 2 annotated transcripts and 2 annotated polypeptides. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | osk: oskar
Homozygous females normally viable and fecund;
homozygous males fertile. Embryos produced by homozygous
females lack pole plasm and fail to produce pole cells; abdominal region remains unsegmented and eventually dies.
Temperature-sensitive period for the germ-line effect is the
last six hours of oogenesis and for abdominal development the
last twelve to fourteen hours (osk8). Homozygous osk germ
cells autonomous in pole cell transplants. Polar cytoplasm
from unfertilized eggs or normal embryos capable of rescuing
pole cell formation if injected at the posterior extremity of
the early embryo and abdominal segmentation, to the extent of
producing viable but sterile adults, if injected into the posterior half of preblastoderm embryos. Embryos produced by
homozygous BicD; osk females are indistinguishable from those
produced by osk alone suggesting that osk+ product is required
for the formation of bicaudal embryos; also required for the
early anterior-posterior gradient of hb expression (Tautz,
1988, Nature 332: 181-84).
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 85B7-85B7
Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 85B-85B (determined by in situ hybridisation)
85B-85B (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | ||||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References In direction of increasing cytology: osk+ tgo- neur- anon-85Cc- neur- anon-85Ca+ Nmdmc- Rel- Nmdmc- Mst85C+ pum- Gene Order (overall orientation not stated) References | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\osk
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 2.9 (longest cDNA) 2.9 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 606 (aa); 69 (kD predicted) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Esterase, SGNH hydrolase-type (IPR013830)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation osk[1] 3R:4,761,400..4,761,400 comment=Insertion of seven bases including an in frame stop codon. Translation terminates at aa180. evidence=experimental point mutation osk[2] 3R:4,761,875..4,761,875 evidence=experimental pr_change=R254@ reported_na_change=C1336T reported_pr_change=R254@ na_change=C4761875T point mutation osk[336] 3R:4,762,180..4,762,180 reported_na_change=C1641T reported_pr_change=Q324@ evidence=experimental pr_change=Q324@ na_change=C4762180T point mutation osk[366] 3R:4,762,180..4,762,180 evidence=experimental pr_change=Q324@ reported_na_change=C1641T reported_pr_change=Q324@ na_change=C4762180T point mutation osk[3] 3R:4,762,891..4,762,891 evidence=experimental pr_change=N561Y reported_na_change=A2352T reported_pr_change=N561Y na_change=A4762891T point mutation osk[5] 3R:4,763,046..4,763,046 evidence=experimental pr_change=R593Q reported_na_change=C2507A reported_pr_change=R593Q na_change=G4763046A point mutation osk[6B10] 3R:4,762,580..4,762,580 comment=site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change evidence=experimental pr_change=S457F reported_pr_change=S457F na_change=C4762580T point mutation osk[6] 3R:4,763,045..4,763,045 evidence=experimental pr_change=R593W reported_na_change=C2506T reported_pr_change=R593W na_change=C4763045T point mutation osk[7] 3R:4,762,565..4,762,565 evidence=experimental pr_change=S452L reported_na_change=C2026T reported_pr_change=S452L na_change=C4762565T point mutation osk[8] 3R:4,762,480..4,762,480 evidence=experimental pr_change=P424S reported_na_change=C1941T reported_pr_change=P424S na_change=C4762480T point mutation osk[9] 3R:4,762,180..4,762,180 evidence=experimental pr_change=Q324@ reported_na_change=C1641T reported_pr_change=Q324@ na_change=C4762180T rescue fragment osk[+t12.75] 3R:4,754,268..4,767,315 linked_to=NcoI-NcoI_rfrag evidence=experimental rescue fragment osk[+t4.3] 3R:4,760,540..4,764,813 linked_to=HpaI-HpaI_rfrag evidence=experimental rescue fragment osk[+t6.45] 3R:4,758,480..4,765,057 linked_to=XhoI-ApaI_rfrag evidence=experimental | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
oogenesis stage,adult stage | female
germarium region 2b
oogenesis stage,adult stage | stage >S8
oocyte | dorsal <&> anterior
embryonic stage | early
oogenesis stage,adult stage | stage >S10
oocyte
oogenesis stage,adult stage
oocyte | posterior
oogenesis stage,adult stage | stage S1-S7
nurse cell
oogenesis stage
oocyte | posterior
oogenesis stage,adult stage
oocyte | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
embryo | early
pole granule
oogenesis stage,adult stage | stage S10
oocyte | posterior
oogenesis stage,adult stage
egg chamber | posterior
oogenesis stage,adult stage | stage >=S9 | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
FlyExpress
- Embryonic expression images (BDGP data)
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele Other Phenotypes Allele Sterility Allele Phenotype manifest in Allele embryonic head, with osk6 embryonic/first instar larval cuticle & embryonic head, with Scer\GAL4nos.UTR.T:Hsim\VP16 embryonic head, with oskΔBRE germline stem cell (with oskGM52) embryonic abdomen (with osk6) egg chamber (with Df(3R)p-XT103) karyosome (with Df(3R)p-XT103) germline stem cell (with osk6) | ||||
Stage(s)
4-6
Stage(s)
9-10
Stage(s)
13-16