HomeGene Dmel\numb
| General Information | ||||
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| Symbol | Dmel\numb | Species | D. melanogaster | |
| Name | numb | Annotation symbol | CG3779 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0002973 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 2L | Recombination map | ||
| Cytogenetic map | 30B3-30B5 | Sequence location | 2L:9,437,513..9,463,293 [+] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | This gene is referred to in FlyBase by the symbol numb (CG3779, FBgn0002973). It has the cytological map location 30B3-30B5. Its sequence location is 2L:9437513..9463293. Its molecular function is described as: protein binding; nucleic acid binding; receptor binding; Notch binding. It is involved in the biological processes described with 21 unique terms, many of which group under: anatomical structure development; cell fate commitment; cell division; regulation of biological process; Notch signaling pathway; sensory organ development; organ development; central nervous system development; regulation of Notch signaling pathway; muscle cell differentiation; neuroblast fate commitment; regulation of neurogenesis; behavior; protein localization; negative regulation of Notch signaling pathway. 61 alleles are reported. The phenotypes of these alleles are annotated with 218 unique terms, many of which group under: hypodermal muscle of larval abdomen; organ system; adult abdominal myoblast; peripheral nervous system; larval abdominal segment 5; abdominal oblique muscle; embryonic nervous system; nervous system; embryonic neuron; abdominal transverse muscle. It has 2 annotated transcripts and 2 annotated polypeptides. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | numb: numb
The numb gene must be able to function in the Drosophila embryo in order for the peripheral sensory neurons to
acquire their correct identity. In the mutants, the precursors of neurons and glial cells in the external sensory (es)
organs are, for the most part, transformed into nonneural support cells; some of the es organs are duplicated. Transformation of neuron precursors into nonneural cells also occurs in
the chordotonal (ch) organs. Precursors of the multiple dendrite (md) neurons undergo similar changes. Muscle development is abnormal in numb mutant alleles; some muscles are
fewer in number than in wild type and show pattern changes
(Uemura et al.).
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 30B3-30B5
Limits computationally determined from genome sequence between P{PZ}scat1&P{PZ}numb03235 and P{EP}Oatp30BEP890
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 30B1-30B12 (determined by in situ hybridisation)
30B1-30B12 (determined by in situ hybridisation)
30B5-30B6 (determined by in situ hybridisation)
30B-30B (determined by in situ hybridisation)
30B5-30B6 30B-30B (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | ||||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\numb
for information on other features
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| Comments on Gene Model | ||||
DGC clone LD25907 appears problematic: incomplete CDS | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 3.5, 3.1 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 556, 515 (aa); 61, 56 (kD predicted) | |||
| Comments | The phosphotyrosine binding domains (PID) of most polypeptides studied preferentially bind peptides containing an NPXpY motif (X = any amino acid), whereas the the PID of numb protein binds peptides with a "YIGPY#" motif (# = a hydrophobic amino acid). The affinity of the PID domain for the peptide increases when the second tyrosine is phosphorylated. The GP(p)Y core motif is required for high-affinity binding. The same amino acids in the numb PID are likely to bind both the phosphorylated and non-phosphorylated forms of the peptide. | |||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Phosphotyrosine interaction region (IPR006020)
NUMB phenylalanine-rich (IPR010449)
Pleckstrin homology-type (IPR011993)
Numb/numb-like (IPR016698)
PDB
- Protein Data Bank. An information portal to biological macromolecular structures
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation numb[S52F] 2L:9,460,578..9,460,578 evidence=experimental na_change=C9460578T pr_change=S52F|numb-PA,S11F|numb-PB reported_na_change=C155T reported_pr_change=S52F | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | |||
| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
larval stage-adult stage
embryonic stage | 0-3 hr
embryonic stage | >=3 hr
adult stage | female
oogenesis stage,adult stage | female
ovary | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
adult stage
external sensory organ precursor cell
adult stage
peripheral nervous system | restricted
embryonic stage | 3-17 hr
embryonic stage
neuroblast
embryonic stage
sensillum precursor
embryonic stage | 0-5 hr
embryonic stage
embryonic ganglion mother cell | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | plasma membrane | |||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
FlyExpress
- Embryonic expression images (BDGP data)
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele Other Phenotypes Allele visible, with Scer\GAL4189 Sterility Allele Phenotype manifest in Allele | ||||
Stage(s)
1-3
Stage(s)
4-6
Stage(s)
7-8
Stage(s)
9-10
Stage(s)
11-12
Stage(s)
13-16