Gene Dmel\Nrg

General Information
Symbol	Dmel\Nrg	Species	D. melanogaster
Name	Neuroglian	Annotation symbol	CG1634
Feature type	protein_coding_gene	FlyBase ID	FBgn0002968
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map	1-23.6
Cytogenetic map	7F2-7F4	Sequence location	X:8,411,406..8,446,150 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Neuroglian is referred to in FlyBase by the symbol Nrg (CG1634, FBgn0002968). It has the cytological map location 7F2-7F4. Its sequence location is X:8411406..8446150. Its molecular function is described as calcium ion binding. It is involved in the biological processes described with 11 unique terms, many of which group under: anatomical structure development; central nervous system development; cell projection organization and biogenesis; neuron differentiation; cell morphogenesis; organ development; system development; synapse organization and biogenesis; cell adhesion; transmembrane receptor protein tyrosine kinase signaling pathway; primary metabolic process. 54 alleles are reported. The phenotypes of these alleles are annotated with 54 unique terms, many of which group under: peripheral nervous system; nervous system; adult segment; abdominal lateral monoscolopidial chordotonal organ lch1; adult brain; larval abdominal segment; larval abdominal segment 5; abdominal segment 7; embryonic/larval tracheal system; embryonic neuron; synapse part; synapse; embryonic dorsal trunk; plasma membrane part; embryonic trachea; abdominal 2 lateral sensillum trichodeum lh1. It has 3 annotated transcripts and 3 annotated polypeptides.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ceb: central brain deranged (J.C. Hall) Extra lobe, in adult brain, of approximately 400 coiled Kenyon-cell fibers next to calyx of mushroom bodies; olfactory learning in adults slightly reduced; memory is normal. Nrg: Neuroglian Encodes a protein that is likely to play a role in neural and glial cell adhesion in the developing Drosophila embryo. Widely expressed in the embryo, particularly on the surfaces of a large subset of neurons and glia that interact with and adhere to one another along the dorsal surface of the developing CNS and within the peripheral nerve roots; the protein can be detected along neuronal axons, but not cell bodies. Three polypeptides of 155, 167, and 180 kd are detected on Western blots; deglycosylation experiments reduce these to a single 155 kd band; removal of N-linked high-mannose oligosaccharides causes a shift to about 162 kd. It is also expressed in a number of non-neuronal tissues such as trachea, hindgut, salivary gland and muscle. Recessive alleles are embryonic lethals and show little or no evidence of Neuroglian expression; the overall structure of the CNS and PNS, and in particular the peripheral nerve roots and CNS axon pathways develop in a relatively normal way in lethal embryos.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 7F2-7F4 Limits computationally determined from genome sequence between P{EP}CG1632^EP1583 and P{EP}Moe^EP1652
Experimentally Determined Cytological Location
Cytogenetic map Notes References 7F1-7F4 (determined by in situ hybridisation) (Peter et al., 2002) 7F-7F (determined by in situ hybridisation) (Schaefer et al., 1999.12.2) 7F1-7F4 (determined by in situ hybridisation) (Schaefer et al., 1999.6.15) 7F1-8A5 (Hall, 1992) 7F1-7F1 (determined by in situ hybridisation) (Hortsch et al., 1990) 7F1-7F1 (determined by in situ hybridisation) (Bieber et al., 1989)
Experimentally Determined Recombination Data
Location	1-23.6 (King et al., 1986) 1-23 (Varnam et al., 1996)
Left of (cM)
Right of (cM)	otu pt (King et al., 1986)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Nrg for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nrg-RA FBtr0071207 NM_078535 4433 1239 Nrg-RB FBtr0071208 NM_167160 4490 1302 Nrg-RC FBtr0071209 NM_206657 4276 1239
Additional Transcript Data & Comments
Reported size (kB)	7.6, 5.7, 5.5 (northern blot) (Hortsch et al., 1990) 5.1 (northern blot) (Bieber et al., 1989)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Nrg-PA FBpp0071154 138.4 1239 5.15 NP_511090 AAF46387 Nrg-PB FBpp0071155 143.6 1302 5.15 NP_727274 AAN09236 Nrg-PC FBpp0089326 138.4 1239 5.15 NP_996380 AAS65287
Additional Polypeptide Data & Comments
Reported size (kD)	1216 (aa); 145-155 (kD predicted) (Bieber et al., 1989) 180 (kD observed) (Hortsch et al., 1990) 1302, 1239 (aa) (Zhao and Hortsch, 1998)
Comments	Neuron-specific 180kD form. (Zhao and Hortsch, 1998) The core 145-155 kD Nrg protein is glycosylated to produce a 167-180 kD protein. Nrg may play a role in neural and glial adhesion in the developing embryo. (Dubreuil et al., 1997)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002968
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin subtype 2 (IPR003598) Immunoglobulin subtype (IPR003599) Fibronectin, type III (IPR003961) Fibronectin, type III subdomain (IPR003962) Immunoglobulin-like (IPR007110) Fibronectin, type III-like fold (IPR008957) Immunoglobulin I-set (IPR013098) Immunoglobulin (IPR013151) Immunoglobulin-like fold (IPR013783) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1CFB
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141818 AA141821 AA202714 AA698810 AE003444 AAF46387 AE003444 AAN09236 AE003444 AAS65287 AF050084 AF050085 AAC28613 AF050085 AAC28614 AI063640 AI260289 AI519567 AJ426788 AJ426790 AW940132 AY058284 AAL13513 BF499347 BI172434 BI213291 BI214938 BI216531 BI231816 BI239412 BI242098 BI358314 BI362520 BI370382 BI564503 BI573722 BI574954 BI585624 BI593211 BI607856 BI608249 BI617045 BI623114 BI627893 BP546676 BP555959 BT024971 CK02514 CK658999 CK659348 CO178505 CO179640 CO186942 CO187549 CO263345 CO263624 CO265803 CO269070 CO269257 CO271484 CO271916 CO274983 CO276121 CO278996 CO283071 CO283550 CO286024 CO288870 CO289639 CO290606 CO293379 CO293974 CO294254 CO295362 CO295596 CO296620 CO298926 CO313137 CO313626 CO315929 CO317056 CO317090 CO322990 CO324953 CO325370 CO325565 CO327994 CO329371 CO330157 CO332909 CO339976 CO340522 CO341278 CO342846 CO345291 CO345423 CZ477335 CZ477336 CZ477570 CZ489079 CZ489080 EC051300 EC051659 EC051931 EC052161 EC052310 EC052443 EC052851 EC053168 EC053653 EC054145 EC054146 EC054150 EC054374 EC054669 EC055409 EC056163 EC056555 EC056640 EC057108 EC058316 EC059318 EC059940 EC059973 EC063393 EC063974 EC067088 EC070200 EC071593 EC076421 EC078821 EC079316 EC081681 EC083841 EC084199 EC086077 EC196859 EC198600 EC201768 EC204929 EC205861 EC208263 EC212717 EC217947 EC218743 EC219630 EC220484 EC223939 EC226811 EC226970 EC228758 EC229084 EC233787 EC236132 EC237100 EC237414 EC241854 EC252085 EC254718 EC256499 EC257579 EC258410 EC258647 EL875306 EL875307 EL884149 EL884150 ES691767 M28231 AAA28728 X76243 CAA53822 X76244 CAA53823

UniProtKB/Swiss-Prot	P20241
UniProtKB/TrEMBL

Maps to
Does NOT map to
Identified with	GH03573 (BDGP Project Members, 2000-) D1035 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 889
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage (Hortsch et al., 1990) embryonic stage \| >=11-12 hr (Bieber et al., 1989) embryonic stage (Hortsch et al., 1990) embryonic stage \| >=9 hr (Hortsch et al., 1990)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference larval stage \| third instar epidermis <of> imaginal disc (Hortsch et al., 1990) larval stage \| third instar neuron <of> imaginal disc (Hortsch et al., 1990) embryonic stage \| >=11-12 hr larval muscle system (Bieber et al., 1989) oogenesis stage,adult stage plasma membrane <of> follicle cell (Dubreuil et al., 1997) larval stage \| third instar neuron<of>imaginal disc (Hortsch et al., 1990) embryonic stage \| >6 hr central nervous system \| restricted (Hortsch et al., 1990) larval stage \| third instar epidermis <of> imaginal disc (Hortsch et al., 1990) embryonic stage \| >=8 hr (Hortsch et al., 1990) embryonic stage \| >=11-12 hr central nervous system \| restricted (Bieber et al., 1989) embryonic stage \| >=7 hr embryonic peripheral nervous system \| restricted (Hortsch et al., 1990) embryonic stage \| >=11-12 hr embryonic/larval trachea (Bieber et al., 1989) embryonic stage \| >=11-12 hr embryonic/larval hindgut (Bieber et al., 1989) embryonic stage \| >=7 hr embryonic peripheral nervous system \| restricted (Hortsch et al., 1990) embryonic stage (Hortsch et al., 1990) embryonic stage \| >=11-12 hr embryonic/larval salivary gland (Bieber et al., 1989) embryonic stage \| >6 hr central nervous system \| restricted (Hortsch et al., 1990)
Marker for

Subcellular Localization
CV Term	plasma membrane (Dubreuil et al., 1997)
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) Untitled Document