• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\nau

General Information
Symbol	Dmel\nau	Species	D. melanogaster
Name	nautilus	Annotation symbol	CG10250
Feature type	protein_coding_gene	FlyBase ID	FBgn0002922
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95A10-95B1	Sequence location	3R:19,538,534..19,544,079 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nautilus is referred to in FlyBase by the symbol nau (CG10250, FBgn0002922). It has the cytological map location 95A10-95B1. Its sequence location is 3R:19538534..19544079. Its molecular function is described as: transcription factor activity; DNA binding; transcription regulator activity. It is involved in the biological processes: regulation of striated muscle development; muscle development; regulation of transcription, DNA-dependent. 12 alleles are reported. The phenotypes of these alleles are annotated with: myoblast; cardioblast; somatic mesoderm; embryonic muscle system; abdominal dorsal oblique muscle 4; abdominal 1 dorsal acute muscle 3; embryonic/larval somatic muscle. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95A10-95B1   Limits computationally determined from genome sequence between P{PZ}l(3)0690606906 and P{PZ}l(3)0468404684
Experimentally Determined Cytological Location
Cytogenetic map Notes References 95A1-95A10 (determined by in situ hybridisation)   (Ranz et al., 1999) 95A-95A (determined by in situ hybridisation)   (Abmayr et al., 1992) 95A-95B (determined by in situ hybridisation)   (Paterson et al., 1991) 95A-95A (determined by in situ hybridisation)   (Michelson et al., 1990)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\nau for information on other features
Comments on Gene Model
	The gene model for nau overlaps the gene model for CG10300 on the opposite strand.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nau-RA FBtr0084472  NM_057302   1534   332
Additional Transcript Data & Comments
Reported size (kB)	1.5 (northern blot) (Paterson et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein nau-PA   FBpp0083863   36.2   332   8.20     NP_476650   AAF56168
Additional Polypeptide Data & Comments
Reported size (kD)	332 (aa); 36 (kD predicted) (Paterson et al., 1991)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002922
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Basic helix-loop-helix dimerisation region bHLH (IPR001092) Myogenic basic muscle-specific protein (IPR002546) Helix-loop-helix DNA-binding (IPR011598) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T01540
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC008201   AE003744 AAF56168   BT022208   CG10250   CO186940   CO196505   CO266346   CO293337   CO306432   CO315162   CO316283   DN153840   EC065563   EC066465   EC196717   EC197949   EC203519   EC212565   EC217123   EC237547   EC244871   EC253753   M68897 AAA28477   X56161 CAA39629
UniProtKB/Swiss-Prot	P22816
UniProtKB/TrEMBL
Maps to	43F9 (Ranz et al., 1999) FBcl0298138 (FlyBase, 1992-)
Does NOT map to
Identified with	IP01012 (Hoskins et al., 2005) D1311 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References point mutation nau[PS6] 3R:19,543,150..19,543,150 comment=Deletion of a base causing a frameshift after amino acid 177. evidence=experimental (Balagopalan et al., 2001) sequence variant nau[188] 3R:19,542,832..19,544,956 comment=Deletion extending from -877 to +1266 evidence=experimental (Balagopalan et al., 2001)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 10501
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  larval muscle system (Corbin et al., 1991)  embryonic stage  larval somatic muscle (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage,larval stage   (Paterson et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)  embryonic stage  somatic mesoderm (Corbin et al., 1991)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage >=11   (Paterson et al., 1991)  embryonic stage | stage >=14  muscle fibre <of> telson (Paterson et al., 1991)  embryonic stage | stage >=14  pharyngeal muscle (Paterson et al., 1991)  embryonic stage | stage >=14   (Paterson et al., 1991)  embryonic stage | stage >=11  larval muscle system (Paterson et al., 1991)  embryonic stage | stage >=14  abdominal 1 ventral longitudinal muscle 1 (Paterson et al., 1991)  embryonic stage | stage >=14 a> abdominal 1 ventral acute muscle 2   (Paterson et al., 1991)  embryonic stage | stage >=14  abdominal 1 ventral acute muscle 1 (Paterson et al., 1991)  embryonic stage | stage >=14  ventral oblique muscle (Paterson et al., 1991)  embryonic stage | stage >=14  abdominal 1..7 ventral oblique muscle 5 (Paterson et al., 1991)  embryonic stage | stage >=14  abdominal 1..7 ventral oblique muscle 4 (Paterson et al., 1991)  embryonic stage | stage >=14  anterior spiracle retractor muscle (Paterson et al., 1991)  embryonic stage | stage >=14  abdominal 2..7 ventral oblique muscle 6 (Paterson et al., 1991)
Marker for
	somatic mesoderm | restricted somatic muscle | restricted
Subcellular Localization
CV Term	nucleus (Paterson et al., 1991)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002922 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0002922 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0002922
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable nau188 lethal, with Scer\GAL4how-24B nauScer\UAS.cKa Other Phenotypes Allele flightless, with Scer\GAL4how-24B nauScer\UAS.cKa Sterility Allele sterile | recessive nau188 female sterile (with Df(3R)nau-11a4) nau188 nauPS6 Phenotype manifest in Allele abdominal dorsal oblique muscle 4 nau188 nauPS6 abdominal 1 dorsal acute muscle 3 nau188 nauPS6 embryonic/larval somatic muscle nau188 nauPS6 abdominal dorsal oblique muscle 4 | germ-line clone nau188 abdominal 1 dorsal acute muscle 3 | germ-line clone nau188 embryonic/larval somatic muscle | germ-line clone nau188 myoblast, with Scer\GAL4how-24B nauScer\UAS.cKa cardioblast, with Scer\GAL4how-24B nauScer\UAS.cKa somatic mesoderm, with Scer\GAL4how-24B nauScer\UAS.cKa embryonic muscle system, with Scer\GAL4how-24B, Scer\GAL4twi.PG naua.Scer\UAS embryonic muscle system naudsRNA.COOH naudsRNA.NH2 naudsRNA.bHLH naudsRNA.cMa
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of nau Class Mutagen Stocks Known lesion nau188 loss of function ethyl methanesulfonate 0 Yes nauPS6 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nau Class Mutagen Stocks Known lesion nauAct5C.PW in vitro construct | regulatory fusion 0 Yes nauGD4473 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes nauScer\UAS.cKa in vitro construct | regulatory fusion 0 Yes naua.Scer\UAS in vitro construct | regulatory fusion 0 Yes naucPa in vitro construct | other 0 Yes naudsRNA.COOH in vitro construct | RNAi 0 Yes naudsRNA.NH2 in vitro construct | RNAi 0 Yes naudsRNA.bHLH in vitro construct | RNAi 0 Yes naudsRNA.cMa in vitro construct | RNAi 0 Yes naudsRNA.cWa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)mbc-R.1
Useful duplication	Dp(3;3)M95A+13
Disrupted in	Df(3R)Exel6195 (Ryder, 2004.4.26) Df(3R)mbc-15A (Erickson et al., 1997) Df(3R)mbc-30 (Erickson et al., 1997) Df(3R)nau-11a4 (Keller et al., 1998, Keller et al., 1998) Df(3R)nau-9 (Keller et al., 1998, Keller et al., 1998)
Not disrupted in	Df(3R)CA15 (Erickson et al., 1997) Df(3R)CA2 (Erickson et al., 1997) Df(3R)mbc-F5.3 (Erickson et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{nau-lacZ.P} Yes P{nau-β-galactosidase} No UAS construct P{GD4473} NA P{UAS-nau.K} NA P{UAS-nau.a} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data nau has been cloned and sequenced and the pattern of RNA expression analysed. (Michelson et al., 1990) Abnormalities in clusters of nau-producing cells appear in the neurogenic mutants, E(spl), N, Dl, bib, neu, mam, and amx, nau and βTub85D, during stage 11, the time of first detectable nau expression. The clusters arise at the correct developmental time and position but contain more cells than wild type clusters. This causes strong ectodermal abnormalities and has effects on mesoderm development. (Corbin et al., 1991) Ecol\lacZ reporter gene constructs have demonstrated that the nau gene product is a transiently expressed nuclear protein that serves as a marker for myogenic precursor cells, or a subset of them, in development. These cells may represent muscle founder cells that are crucial in organising and establishing the precise muscle pattern in each segment of the body plan. nau is a distinctive gene and is not a member of a multigene family. (Paterson et al., 1991) nau RNA expression during development has been studied. (Abmayr et al., 1992) wg acts as an inductive signal that influences the expression of nau in the ventral mesoderm. (Ranganayakulu et al., 1996) Ectopic expression of nau in developing cardioblasts suggests nau induces myogenic differentiation in vivo and may affect the myogenic differentiation program of specific muscle fibres. (Keller et al., 1997) Embryos lacking both maternal and zygotic nau function lack a distinct set of muscle fibres. This muscle loss is tolerated, however, such that the loss of both maternal and zygotic nau function does not result in lethality at any stage of development. (Balagopalan et al., 2001)
Gene Ontology: Function, Process & Cellular Component ( 7 )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1991.8.1) inferred from electronic annotation with InterPro:IPR002546 (FlyBase Curators et al., 2004-)
transcription factor activity traceable author statement (Bate et al., 1999)
transcription regulator activity inferred from electronic annotation with InterPro:IPR001092, InterPro:IPR011598 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
muscle development traceable author statement (Dworak and Sink, 2002) inferred from electronic annotation with InterPro:IPR002546 (FlyBase Curators et al., 2004-)
regulation of striated muscle development traceable author statement (Bate et al., 1999)
regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR002546 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
nucleus traceable author statement (Bate et al., 1999) non-traceable author statement (Swiss-Prot Project Members, 1991.8.1) inferred from electronic annotation with InterPro:IPR001092, InterPro:IPR002546, InterPro:IPR011598 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nau allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67737 Drosophila PIMRider - The Drosophila Protein Interaction map • 521355
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18746 Dere\GG12413 Dgri\GH13904 Dmoj\GI24830 Dper\GL13832 Dpse\GA10192 Dsec\GM23547 Dsim\GD18361 Dvir\GJ24474 Dwil\GK22777 Dyak\GE23932
Curated drosophilid orthologs	Dbuz\nau (Ranz et al., 1999) Dpse\GA10192   Drep\nau (Ranz et al., 1999) Dvir\nau (Ranz et al., 1999)
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000013830 Anopheles gambiae (Ensembl) ENSBTAP00000038425 Bos taurus (Ensembl) ENSCAFP00000008695 Canis familiaris (Ensembl) NEWSINFRUP00000164711 Takifugu rubripes (Ensembl) ENSGALP00000010017 Gallus gallus (Ensembl) ENSP00000228644 Homo sapiens (Ensembl) ENSMMUP00000015315 Macaca mulatta (Ensembl) ENSMODP00000025626 Monodelphis domestica (Ensembl) ENSPTRP00000008937 Pan troglodytes (Ensembl) ENSRNOP00000006453 Rattus norvegicus (Ensembl) GSTENP00003954001 Tetraodon nigroviridis (Ensembl) ENSXETP00000031778 Xenopus tropicalis (Ensembl) WBGene00034231 Caenorhabditis briggsae (Wormbase) ZDB-GENE-980526-561 Danio rerio (Zfin) MGI:97252 Mus musculus (Informatics)
Functional Complementation between Species
Functionally complements	Cele\hlh-1 (Zhang et al., 1999)
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
VDRC	v51311 w1118; P{GD4473}v51311
Genomic Clones ( 2 )
	BACR11E07 BACR16I18
cDNA Clones ( 9 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP01012
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	EK251028 EK239541 EK005020 EK146118 EK202615 EK264353 EK024905 EK109323
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0002922 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0002922
Antibody Information
	polyclonal (Paterson et al., 1991)
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
	nau is not required for the formation of muscle precursors, but rather plays a role in the differentiation of a subset of muscle precursors into mature muscle fibres in the developing embryo. (Keller et al., 1998) nau has a crucial role in embryonic muscle formation. (Misquitta and Paterson, 1999)
Comments About Molecular Function
Other Comments
	Isolated from a genomic library at low stringency using a mouse MyoD and rat myogenin probe. (Michelson et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057302 RefSeq (Proteins) • NP_476650 DDBJ / EMBL / GenBank • AC008201 AE003744 AAF56168 M68897 AAA28477 X56161 CAA39629 UniProtKB/Swiss-Prot • P22816
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Basic helix-loop-helix dimerisation region bHLH (IPR001092) Myogenic basic muscle-specific protein (IPR002546) Helix-loop-helix DNA-binding (IPR011598) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T01540
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 67737 DEDB - Drosophila exon database: splicing graphs • 10501 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0002922 Drosophila PIMRider - The Drosophila Protein Interaction map • 521355 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002922 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0002922 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0002922 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0002922 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0002922 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000013830 Anopheles gambiae (Ensembl) ENSBTAP00000038425 Bos taurus (Ensembl) ENSCAFP00000008695 Canis familiaris (Ensembl) NEWSINFRUP00000164711 Takifugu rubripes (Ensembl)