Gene Dmel\HLHmδ

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General Information

Symbol

Dmel\HLHmδ

Species

D. melanogaster

Name

E(spl) region transcript mδ

Annotation symbol

CG8328

Feature type

protein_coding_gene

FlyBase ID

FBgn0002734

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

3-89.1

Cytogenetic map

96F9-96F9

Sequence location

3R:21,823,343..21,824,358 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene E(spl) region transcript mδ is referred to in FlyBase by the symbol HLHmδ (CG8328, FBgn0002734). It has the cytological map location 96F9. Its sequence location is 3R:21823343..21824358. Its molecular function is described as: transcription factor activity; DNA binding; transcription repressor activity; specific transcriptional repressor activity. It is involved in the biological processes: negative regulation of transcription from RNA polymerase II promoter; Notch signaling pathway; negative regulation of transcription; nervous system development; compound eye development. 7 alleles are reported. The phenotypes of these alleles are annotated with: scutellar bristle; wing vein; wing margin bristle; macrochaeta; photoreceptor cell R8; eye; microchaeta; adult thorax; ommatidium. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96F9-96F9

Limits computationally determined from genome sequence between P{PZ}l(3)rQ197^rQ197 and P{lacW}scrib^j7B3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

96F11-96F14

(Nellesen, 1998.12.18)

Experimentally Determined Recombination Data

Location

3-89.1

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: HLHmδ+ HLHmγ- HLHmβ- mα+ m1? m2? HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+

(Ligoxygakis et al., 1998)

Gene Order (overall orientation not stated)

References

Overall orientation not stated: HLHmδ- Nf1+

(Tyler, 2000.3.6)

Overall orientation not stated: HLHmδ+ HLHmγ+ HLHmβ- mα+ m1+ m2- HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+

(Lai et al., 2000)

Gene Model & Products

Please see the GBrowse view of Dmel\HLHmδ for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

HLHmdelta-RA

FBtr0084954

NM_079779

1016

173

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

HLHmdelta-PA

FBpp0084328

20.2

173

7.19

NP_524503

AAF56544

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

The predicted protein products of the seven HLH genes in the E(spl) complex exhibit a great degree of sequence similarity extending over the first 120 amino acids of the proteins. The carboxy terminus is unique for each except for the terminal 4 amino acids, which are identical in all of the proteins.

(Delidakis and Artavanis-Tsakonas, 1992)

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0002734

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Basic helix-loop-helix dimerisation region bHLH (IPR001092)

Orange (IPR003650)

Helix-loop-helix DNA-binding (IPR011598)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q01071

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

IP01594

(Hoskins et al., 2005)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

protein binding site

HLHmdelta-protein_bind-1
3R:21,822,005..21,822,011

bound_moiety=Su(H)-XP
comment=Su(H) binding site mdelta-3
evidence=experimental

(Eastman et al., 1997, Delidakis, 1997.7.18)

protein binding site

HLHmdelta-protein_bind-2
3R:21,822,027..21,822,033

bound_moiety=Su(H)-XP
comment=Su(H) binding site mdelta-2
evidence=experimental

(Eastman et al., 1997, Delidakis, 1997.7.18)

protein binding site

HLHmdelta-protein_bind-3
3R:21,822,569..21,822,575

bound_moiety=Su(H)-XP
comment=Su(H) binding site mdelta-1
evidence=experimental

(Eastman et al., 1997, Delidakis, 1997.7.18)

rescue fragment

HLHmδ[+t6]
3R:21,816,363..21,824,637

linked_to=EcoRI-EcoRI_rfrag

(Schrons et al., 1992)

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10869

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0002734

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0002734

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0002734

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for HLHmδ

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pharate adult stage, with Scer\GAL4^h-H10

HLHmδ^{Scer\UAS.cdCa}

Other Phenotypes

Allele

visible, with Scer\GAL4^32B

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^455.2

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^sca-537.4

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^h-1J3

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^ptc-559.1

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^pnr-MD237

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^ap-md544

HLHmδ^{Scer\UAS.cdCa}

visible, with Scer\GAL4^h-H10

HLHmδ^{Scer\UAS.cdCa}

Phenotype manifest in

Allele

scutellar bristle, with Scer\GAL4^ptc-559.1

HLHmδ^{Scer\UAS.cdCa}

wing vein, with Scer\GAL4^32B

HLHmδ^{Scer\UAS.cdCa}

wing margin bristle, with Scer\GAL4^32B

HLHmδ^{Scer\UAS.cdCa}

scutum & microchaeta, with Scer\GAL4^32B

HLHmδ^{Scer\UAS.cdCa}

scutum & microchaeta, with Scer\GAL4^ap-md544

HLHmδ^{Scer\UAS.cdCa}

scutum & macrochaeta, with Scer\GAL4^ap-md544

HLHmδ^{Scer\UAS.cdCa}

scutellar bristle, with Scer\GAL4^455.2

HLHmδ^{Scer\UAS.cdCa}

scutum & macrochaeta, with Scer\GAL4^h-1J3

HLHmδ^{Scer\UAS.cdCa}

macrochaeta, with Scer\GAL4^sca-537.4

HLHmδ^{Scer\UAS.cdCa}

photoreceptor cell R8, with Scer\GAL4^sca-109-68

HLHmδ^{Scer\UAS.cdCa}

eye, with Scer\GAL4^sca-109-68

HLHmδ^{Scer\UAS.cdCa}

macrochaeta, with Scer\GAL4^pnr-MD237

HLHmδ^{Scer\UAS.cdCa}

microchaeta, with Scer\GAL4^pnr-MD237

HLHmδ^{Scer\UAS.cdCa}

adult thorax, with Scer\GAL4^pnr-MD237

HLHmδ^{Scer\UAS.cdCa}

eye, with Scer\GAL4^h-H10

HLHmδ^{Scer\UAS.cdCa}

ommatidium, with Scer\GAL4^h-H10

HLHmδ^{Scer\UAS.cdCa}

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of HLHmδ	Class	Mutagen	Stocks	Known lesion
HLHmδ^e01428		piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Allele of HLHmδ	Mutagen	Stocks	Known lesion
HLHmδ^+t13	in vitro construct \| genomic fragment	0	Yes
HLHmδ^+t6	in vitro construct \| genomic fragment	0	Yes
HLHmδ^Act5C.PG	in vitro construct \| regulatory fusion	0	Yes
HLHmδ^GD4458	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
HLHmδ^{Scer\UAS.T:Hsim\VP16}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
HLHmδ^{Scer\UAS.cdCa}	in vitro construct \| regulatory fusion	0	Yes