Gene Dmel\Bj1

General Information
Symbol	Dmel\Bj1	Species	D. melanogaster
Name	Bj1 protein	Annotation symbol	CG10480
Feature type	protein_coding_gene	FlyBase ID	FBgn0002638
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	65A1-65A1	Sequence location	3L:5,923,359..5,925,873 [-]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Bj1 protein is referred to in FlyBase by the symbol Bj1 (CG10480, FBgn0002638). It has the cytological map location 65A1. Its sequence location is 3L:5923359..5925873. Its molecular function is described as: chromatin binding; Ran guanyl-nucleotide exchange factor activity. It is involved in the biological processes: central nervous system development; regulation of neurogenesis; regulation of nucleocytoplasmic transport; chromosome condensation; mitotic chromosome condensation. 15 alleles are reported. The phenotypes of these alleles are annotated with: anterior fascicle; EL neuron; central nervous system; eye; eye photoreceptor cell; ommatidium. It has 3 annotated transcripts and 3 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 65A1-65A1 Limits computationally determined from genome sequence between P{PZ}vn¹⁰⁵⁶⁷ and P{PZ}loj⁰⁴⁰²⁶
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64F-64F (Eggert et al., 1998) 64F-64F (determined by in situ hybridisation) (Frasch, 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Bj1 for information on other features
Comments on Gene Model
	Multiple ESTs homologous to non-coding strand of this gene
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Bj1-RA FBtr0077074 NM_079219 2368 547 Bj1-RB FBtr0077075 NM_168151 2202 547 Bj1-RC FBtr0077076 NM_168152 2143 547
Additional Transcript Data & Comments
Reported size (kB)	2.2 (northern blot) (Frasch, 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Bj1-PA FBpp0076782 58.9 547 8.06 NP_523943 AAF50726 Bj1-PB FBpp0076783 58.9 547 8.06 NP_729118 AAF50727 Bj1-PC FBpp0076784 58.9 547 8.06 NP_729119 AAN12235
Additional Polypeptide Data & Comments
Reported size (kD)	547 (aa); 68 (kD) (Frasch, 1991) 68 (kD) (Ohtsubo et al., 1991)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002638
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Regulator of chromosome condensation, RCC1 (IPR000408) Regulator of chromosome condensation/beta-lactamase-inhibitor protein II (IPR009091)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201925 AA202046 AA202412 AA202572 AA246416 AA246799 AA263792 AA263823 AA263867 AA264127 AA264354 AA264974 AA390302 AA391440 AA392124 AA392653 AA438687 AA439116 AA439527 AA440262 AA440520 AA441094 AA536321 AA536574 AA536589 AA538702 AA538863 AA538929 AA539104 AA540125 AA540267 AA540374 AA541224 AA735561 AA802172 AA803831 AA816290 AA816319 AA816662 AA817289 AA817314 AA820609 AA820733 AA820835 AA940797 AA940818 AA941005 AA941523 AA941582 AA941755 AA941891 AA942247 AA942517 AA942544 AA942593 AA942630 AA949206 AA949371 AA949476 AA950037 AA950095 AA950504 AA950900 AA951784 AA952016 AA952114 AA978747 AA978830 AA979112 AA979243 AA979494 AA979687 AA990869 AE003564 AAF50726 AE003564 AAF50727 AE003564 AAN12235 AI061708 AI061905 AI297714 AI455275 AI456253 AI456806 AI512048 AI512309 AI512311 AI512562 AI512849 AI514318 AI514493 AI514687 AI514929 AI515664 AI515717 AI516212 AI516795 AI518522 AI518563 AI519616 AI520420 AI520436 AI530992 AI533251 AI533673 AI542615 AI543418 AI546288 AQ073074 AY070540 AAL48011 BE975813 BF498731 BF502043 BG635349 BI163146 BI163843 BI164887 BI165324 BI166193 BI166491 BI167260 BI167429 BI167837 BI168580 BI171513 BI213919 BI214063 BI214492 BI215702 BI216042 BI216503 BI228318 BI231771 BI232283 BI235103 BI238011 BI239663 BI241681 BI242218 BI354289 BI354378 BI356139 BI356339 BI358551 BI362307 BI364400 BI365987 BI366771 BI372067 BI482215 BI485899 BI564162 BI576052 BI577491 BI577492 BI578552 BI580481 BI584682 BI609492 BI626316 BI635454 BI635610 BI636903 BI637597 BI640516 BP554761 BP554762 BQ102680 CK131969 CL527882 CO156807 CO177152 CO178753 CO292438 CO293976 CO300102 CO308408 EC072409 EC079017 EC201121 EC201398 EC209749 EC224388 EC230081 EC230341 EC238200 EC243039 EC264903 X58530 CAA41417

UniProtKB/Swiss-Prot	P25171
UniProtKB/TrEMBL

Maps to
Does NOT map to
Identified with	LD22520 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References complex substitution Bj1[XX138] 3L:5,924,136..5,924,581 comment=Contains a large deletion and an insertion of two alanines. This results in a frame shift at the end of the fourth RCC1 repeat and a premature stop codon after a run of 33 additional novel amino acids. evidence=experimental (Shi and Skeath, 2004) deletion Bj1[FF32] 3L:5,925,091..5,925,105 comment=15 base in-frame deletion at the beginning of the second RCC1 repeat. evidence=experimental (Shi and Skeath, 2004) point mutation Bj1[837] 3L:5,925,104..5,925,104 evidence=experimental na_change=G5925104A pr_change=G93E\|Bj1-PA,G93E\|Bj1-PB,G93E\|Bj1-PC reported_na_change=G770A reported_pr_change=G?E (Shi and Skeath, 2004) point mutation Bj1[UU92] 3L:5,924,760..5,924,760 evidence=experimental na_change=G5924760A pr_change=G208R\|Bj1-PA,G208R\|Bj1-PB,G208R\|Bj1-PC reported_na_change=G1114A reported_pr_change=G?R (Shi and Skeath, 2004) point mutation Bj1[VV179] 3L:5,924,160..5,924,160 evidence=experimental na_change=C5924160T pr_change=P388L\|Bj1-PA,P388L\|Bj1-PB,P388L\|Bj1-PC reported_na_change=C1714T reported_pr_change=P?L (Shi and Skeath, 2004) sequence variant Bj1[OreR]-1 3L:5,925,049..5,925,049 comment=One of three amino acid polymorphisms in the Bj1 CDS detected in the Oregon R strain relative to Canton S. evidence=experimental na_change=C4382R pr_change=S111R\|Bj1-PB,S111R\|Bj1-PC,S111R\|Bj1-PA reported_pr_change=S111R (Shi and Skeath, 2004) sequence variant Bj1[OreR]-2 3L:5,923,897..5,923,897 comment=One of three amino acid polymorphisms in the Bj1 CDS detected in the Oregon R strain relative to Canton S. evidence=experimental na_change=A5534G pr_change=K476E\|Bj1-PB,K476E\|Bj1-PC,K476E\|Bj1-PA reported_pr_change=K476E (Shi and Skeath, 2004) sequence variant Bj1[OreR]-3 3L:5,923,854..5,923,854 comment=One of three amino acid polymorphisms in the Bj1 CDS detected in the Oregon R strain relative to Canton S. evidence=experimental na_change=T5577C pr_change=V490A\|Bj1-PB,V490A\|Bj1-PC,V490A\|Bj1-PA reported_pr_change=V490A (Shi and Skeath, 2004)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 5081
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage \| early (Frasch, 1991) oogenesis stage,adult stage nurse cell (Frasch, 1991) embryonic stage neuroblast (Frasch, 1991) oogenesis stage,adult stage oocyte (Frasch, 1991)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference embryonic stage (Trieselmann and Wilde, 2002) embryonic stage (Trieselmann and Wilde, 2002) embryonic stage embryonic central nervous system (Frasch, 1991) embryonic stage neuroblast (Frasch, 1991) oogenesis stage,adult stage oocyte (Frasch, 1991) larval stage embryonic/larval salivary gland (Frasch, 1991) oogenesis stage,adult stage nurse cell (Frasch, 1991)
Marker for

Subcellular Localization
CV Term	nucleus nucleosome polytene chromosome chromatin (