• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Dmel\HLHm3

General Information
Symbol	Dmel\HLHm3	Species	D. melanogaster
Name	E(spl) region transcript m3	Annotation symbol	CG8346
Feature type	protein_coding_gene	FlyBase ID	FBgn0002609
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	3R	Recombination map	3-89.1
Cytogenetic map	96F10-96F10	Sequence location	3R:21,847,437..21,848,858 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene E(spl) region transcript m3 is referred to in FlyBase by the symbol HLHm3 (CG8346, FBgn0002609). It has the cytological map location 96F10. Its sequence location is 3R:21847437..21848858. Its molecular function is described as: transcription factor activity; DNA binding; specific transcriptional repressor activity; transcription regulator activity. It is involved in the biological processes: Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter; compound eye development; regulation of transcription, DNA-dependent. 6 alleles are reported. The phenotype of these alleles is annotated with scutellar bristle. It has one annotated transcript and one annotated polypeptide.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96F10-96F10   Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96F11-96F14   (Nellesen, 1998.12.18)
Experimentally Determined Recombination Data
Location	3-89.1
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: HLHmδ+ HLHmγ- HLHmβ- mα+ m1? m2? HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+ (Ligoxygakis et al., 1998) In direction of increasing cytology: HLHmβ- mα+ m1+ m2- HLHm3+ m4- HLHm5- m6+ HLHm7+ (Wurmbach et al., 1999) Gene Order (overall orientation not stated) References Overall orientation not stated: HLHmδ+ HLHmγ+ HLHmβ- mα+ m1+ m2- HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+ (Lai et al., 2000)
Gene Model & Products
Please see the GBrowse view of Dmel\HLHm3 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) HLHm3-RA FBtr0084958  NM_079785   1422   224
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Knust et al., 1987)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein HLHm3-PA   FBpp0084332   25.0   224   8.23     NP_524509   AAF56550
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002609
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Basic helix-loop-helix dimerisation region bHLH (IPR001092) Orange (IPR003650) Helix-loop-helix DNA-binding (IPR011598)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003754 AAF56550   AF115454   AY094858 AAM11211   AY779906 AAV59048   AY779907 AAV59060   AY779908 AAV59072   AY779909 AAV59084   AY779910 AAV59096   AY779911 AAV59108   AY779912 AAV59120   AY779913 AAV59132   AY779914 AAV59144   AY779915 AAV59156   AY779916 AAV59168   AY779917 AAV59180   AY779918 AAV59192   AY779919 AAV59204   AY779920 AAV59216   AY779921 AAV59228   AY906259 AAX60605   AY906260 AAX60606   AY906261 AAX60607   AY906262 AAX60608   AY906263 AAX60609   AY906264 AAX60610   AY906265 AAX60611   AY906266 AAX60612   AY906267 AAX60613   AY906268 AAX60614   AY906269 AAX60615   AY906270 AAX60616   AY906271 AAX60617   AY906272 AAX60618   AY906273 AAX60619   AY906274 AAX60620   AY906275 AAX60621   AY906276 AAX60622   AY906277 AAX60623   AY906278 AAX60624   AY906279 AAX60625   AY906280 AAX60626   AY906281 AAX60627   AY906282 AAX60628   AY906283 AAX60629   AY906284 AAX60630   AY906285 AAX60631   AY906286 AAX60632   AY906287 AAX60633   AY906288 AAX60634   AY906289 AAX60635   AY906290 AAX60636   AY906291 AAX60637   AY906292 AAX60638   AY906293 AAX60639   AY906294 AAX60640   AY906295 AAX60641   AY906296 AAX60642   AY906297 AAX60643   AY906298 AAX60644   AY906299 AAX60645   AY906300 AAX60646   AY906301 AAX60647   AY906302 AAX60648   AY906303 AAX60649   AY906304 AAX60650   AY906305 AAX60651   AY906306 AAX60652   AY906307 AAX60653   AY906308 AAX60654   AY906309 AAX60655   AY906310 AAX60656   AY906311 AAX60657   AY906312 AAX60658   AY906313 AAX60659   AY906314 AAX60660   AY906315 AAX60661   AY906316 AAX60662   AY906317 AAX60663   AY906318 AAX60664   AY906319 AAX60665   AY906320 AAX60666   AY906321 AAX60667   AY906322 AAX60668   AY906323 AAX60669   AY906324 AAX60670   AY906325 AAX60671   AY906326 AAX60672   AY906327 AAX60673   AY906328 AAX60674   AY906329 AAX60675   AY906330 AAX60676   AY906331 AAX60677   AY906332 AAX60678   AY906333 AAX60679   AY906334 AAX60680   AY906335 AAX60681   AY906336 AAX60682   AY906337 AAX60683   AY906338 AAX60684   AY906339 AAX60685   AY906340 AAX60686   AY906341 AAX60687   AY906342 AAX60688   AY906343 AAX60689   AY906344 AAX60690   AY906345 AAX60691   AY906346 AAX60692   AY906347 AAX60693   AY906348 AAX60694   AY906349 AAX60695   BI167978   BI213666   CO261931   CO263111   CO268629   CO268672   CO275920   CO291004   CO303109   CO303614   CO309644   CZ472012   EC059226   EC068476   EC070869   EC075926   EC082891   EC090547   EC201948   EC202745   EC203058   EC207900   EC214380   EC217037   EC217250   EC231206   EC242793   M96165 AAA28908   X67046 CAA47431
UniProtKB/Swiss-Prot	Q01068
UniProtKB/TrEMBL	Q56LA3 Q56LC9 Q56LE8 Q56LI3 Q5S455 Q5S479 Q5S4J9
Maps to
Does NOT map to
Identified with	RE19116 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References rescue fragment HLHm3[+t7.2] 3R:21,846,110..21,853,423 linked_to=EcoRI-EcoRI_rfrag (Schrons et al., 1992)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 10879
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 13  embryonic/larval midgut | restricted (Knust et al., 1987)  embryonic stage | 6-14 hr   (Knust et al., 1987)  embryonic stage | stage 13  Malpighian tubule (Knust et al., 1987)  adult stage | female   (Knust et al., 1987)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002609 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0002609 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0002609 FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for HLHm3 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL432B HLHm3Scer\UAS.cLa Other Phenotypes Allele visible, with Scer\GAL432B HLHm3Scer\UAS.cLa visible, with Scer\GAL4455.2 HLHm3Scer\UAS.cLa visible, with Scer\GAL4h-1J3 HLHm3Scer\UAS.cLa visible, with Scer\GAL4ap-md544 HLHm3Scer\UAS.cLa Phenotype manifest in Allele scutum & microchaeta, with Scer\GAL432B HLHm3Scer\UAS.cLa scutum & microchaeta, with Scer\GAL4ap-md544 HLHm3Scer\UAS.cLa scutum & macrochaeta, with Scer\GAL4ap-md544 HLHm3Scer\UAS.cLa scutellar bristle, with Scer\GAL4455.2 HLHm3Scer\UAS.cLa scutum & macrochaeta, with Scer\GAL4h-1J3 HLHm3Scer\UAS.cLa
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of HLHm3 Class Mutagen Stocks Known lesion HLHm3d01076 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of HLHm3 Class Mutagen Stocks Known lesion HLHm3+t7.2 in vitro construct | genomic fragment 0 Yes HLHm3Act5C.PG in vitro construct | regulatory fusion 0 Yes HLHm3Scer\UAS.cLa in vitro construct | regulatory fusion 0 Yes HLHm3dsRNA.cDa in vitro construct | RNAi 0 Yes HLHm3dsRNA.cDb in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)Espl9   Df(3R)Pr4
Useful duplication	Dp(3;3)M95A+13
Disrupted in	Df(3R)E(spl)-rv27 (Tietze et al., 1993, Heitzler et al., 1996, de Celis et al., 1996) Df(3R)Espl4 (Heitzler et al., 1996) Df(3R)Exel6204 (Ryder, 2004.4.26) Df(3R)P11 (Nagel et al., 2000) Df(3R)Pr4 (Nagel et al., 2000) Df(3R)X10 (Nagel et al., 2000) Df(3R)boss16 (Heitzler et al., 1996) Df(3R)grob32.2 (Ligoxygakis et al., 1998, Heitzler e