Gene Dmel\m2

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General Information

Symbol

Dmel\m2

Species

D. melanogaster

Name

E(spl) region transcript m2

Annotation symbol

CG6104

Feature type

protein_coding_gene

FlyBase ID

FBgn0002592

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

3-89.1

Cytogenetic map

96F10-96F10

Sequence location

3R:21,842,231..21,843,192 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene E(spl) region transcript m2 is referred to in FlyBase by the symbol m2 (CG6104, FBgn0002592). It has the cytological map location 96F10. Its sequence location is 3R:21842231..21843192. Its molecular function is unknown. It is involved in the biological process Notch signaling pathway. 4 alleles are reported. The phenotypes of these alleles are annotated with: sensory mother cell; wing vein L5; anterior dorsocentral bristle; posterior dorsocentral bristle; microchaeta. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96F10-96F10

Limits computationally determined from genome sequence between P{PZ}l(3)rQ197^rQ197 and P{lacW}scrib^j7B3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

96F11-96F14

(Wurmbach, 1998.8.16)

Experimentally Determined Recombination Data

Location

3-89.1

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: HLHmδ+ HLHmγ- HLHmβ- mα+ m1? m2? HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+

(Ligoxygakis et al., 1998)

In direction of increasing cytology: HLHmβ- mα+ m1+ m2- HLHm3+ m4- HLHm5- m6+ HLHm7+

(Wurmbach et al., 1999)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\m2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

m2-RA

FBtr0084981

NM_079784

962

218

Additional Transcript Data & Comments

Reported size (kB)

0.9 (northern blot)

(Knust et al., 1987)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

m2-PA

FBpp0084354

24.1

218

6.94

NP_524508

AAF56549

Additional Polypeptide Data & Comments

Reported size (kD)

218 (aa)

(Wurmbach et al., 1999)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0002592

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O97177

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

RE15854

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10878

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

larval stage | third instar

eye disc

(Wurmbach et al., 1999)

embryonic stage | stage 9

neurectoderm

(Wurmbach et al., 1999)

embryonic stage

(Knust et al., 1987)

embryonic stage | stage 10,11

mesoderm | presumptive

(Wurmbach et al., 1999)

embryonic stage | 14-20 hr

(Knust et al., 1987)

larval stage | third instar

morphogenetic furrow | posterior to

(Wurmbach et al., 1999)

larval stage | third instar

dorsal mesothoracic disc | restricted

(Wurmbach et al., 1999)

larval stage | third instar

morphogenetic furrow | vicinity of

(Wurmbach et al., 1999)

larval stage | third instar

proneural cluster <of> dorsal mesothoracic disc

(Wurmbach et al., 1999)

larval stage | third instar

proneural cluster

(Wurmbach et al., 1999)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0002592

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0002592

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0002592

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for m2

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for m2

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

visible, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

visible, with Scer\GAL4^Bx-MS1096

m2^Scer\UAS.cLa

Phenotype manifest in

Allele

sensory mother cell, with Scer\GAL4^Bx-MS1096

m2^Scer\UAS.cLa

wing vein L5, with Scer\GAL4^Bx-MS1096

m2^Scer\UAS.cLa

anterior dorsocentral bristle, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

posterior dorsocentral bristle, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

macrochaeta & adult head, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

macrochaeta & mesothoracic tergum, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

microchaeta, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

sensory mother cell, with Scer\GAL4^sca-537.4

m2^Scer\UAS.cLa

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of m2	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of m2	Mutagen	Stocks	Known lesion
m2^GD11819	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
m2^Scer\UAS.cLa	in vitro construct \| regulatory fusion	0	Yes
m2^dsRNA.cDa	in vitro construct \| RNAi	0	Yes
m2^dsRNA.cDb	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)Espl9

Df(3R)Pr4

Useful duplication

Dp(3;3)M95A⁺13

Disrupted in

Df(3R)E(spl)-rv27

(Tietze et al., 1993)

Df(3R)Exel6204

(Ryder, 2004.4.26)

Df(3R)P11

(Nagel et al., 2000)

Df(3R)Pr4

(Nagel et al., 2000)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{m2-lacZ.L}

UAS construct

P{GD11819}

P{UAS-m2.L}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

E(spl) bHLH proteins are turned on in cells which are inhibited from becoming neural by signals from the delaminating neuroblast.

(Jennings et al., 1995)

The bristle loss phenotype of H mutants can be suppressed by deleting components of the E(spl)-complex. The degree of suppression depends on both the number and identity of E(spl)-complex transcription units removed.

(Bang et al., 1995)

Overexpression of m2 or m6 interferes with N dependent cell fate decisions in adult PNS development. Overexpression of m2 potentiates lateral inhibition.

(Lai et al., 2000)

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

Notch signaling pathway

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

m2 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68055

Orthologs

Genome-wide drosophilid orthologs

Dana\GF18224

Dere\GG12206

Dgri\GH18147

Dmoj\GI22909

Dper\GL22020

Dpse\GA19354

Dsec\GM10205

Dsim\GD18155

Dvir\GJ23322

Dwil\GK12808

Dyak\m2

Curated drosophilid orthologs

Dhyd\m2

Dpse\GA19354

Dsim\m2

Dyak\m2

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v30115

w¹¹¹⁸; P{GD11819}v30115

v30117

w¹¹¹⁸; P{GD11819}v30117/CyO

Genomic Clones ( 2 )

cDNA Clones ( 2 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE15854

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE13230

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0002592

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0002592

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

The distinct expression patterns of genes of the E(spl) complex in imaginal tissues depend to a significant degree on the capacity of their transcriptional cis-regulatory apparatus to respond selectively to direct proneural and Su(H)-mediated activation, often in a subset of the territories and cells in which proneural and Su(H) regulation is occurring.

(Nellesen et al., 1999)

m2 and m6 are direct targets of regulation by the N-activated Su(H) transcription factor. Overall orientation not stated: HLHmδ+ HLHmγ+ HLHmβ- mα+ m1+ m2- HLHm3+ m4- HLHm5- m6+ HLHm7+ E(spl)+ gro+

(Lai et al., 2000)

Other Comments

Arrangement and sequence of E(spl)-complex genes in D.melanogaster and D.hydei revealed that the E(spl)-gene, and the structure of complex are highly conserved, suggesting that each individual gene, as well as the organization of the complex, is of functional importance.

(Maier et al., 1993)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079784

RefSeq (Proteins)

•

NP_524508

DDBJ / EMBL / GenBank

UniProtKB/Swiss-Prot

•

O97177

UniProtKB/TrEMBL

•

Other Crossreferences

Linkouts

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for m2

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68055

DEDB - Drosophila exon database: splicing graphs

•

10878

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0002592

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0002592

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0002592

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0002592

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0002592

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0002592

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0002592

PANTHER - Protein classification by function, families, and pathways

•

FBgn0002592

Synonyms & Secondary IDs ( 4 )

Reported As

Symbol Synonym

CG6104

E(spl)m2

(Lai et al., 2005, Rebeiz et al., 2002, Lai, 2002, Lai et al., 2000, Wurmbach, 1998.8.16)

(Dorner et al., 2006, Schlatter and Maier, 2005)

Name Synonym

E(spl) region transcript m2

Secondary FlyBase IDs

References ( 27 )

Generate a list of

All references relating to this gene ( 27 )

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Recent research papers ( 1 )

Dorner et al., 2006, Proc. Natl. Acad. Sci. USA 103(32): 11880--11885: A genomewide screen for components of the RNAi pathway in Drosophila cultured cells. [FBrf0192146]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2006

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