Gene Dmel\Lam

General Information
Symbol	Dmel\Lam	Species	D. melanogaster
Name	Lamin	Annotation symbol	CG6944
Feature type	protein_coding_gene	FlyBase ID	FBgn0002525
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	25E6-25E6	Sequence location	2L:5,542,527..5,546,642 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Lamin is referred to in FlyBase by the symbol Lam (CG6944, FBgn0002525). It has the cytological map location 25E6. Its sequence location is 2L:5542527..5546642. Its molecular function is described as: protein binding; structural molecule activity. It is involved in the biological processes: mitotic nuclear envelope reassembly; terminal branching, open tracheal system; nuclear migration; central nervous system development; compound eye morphogenesis; chitin-based cuticle development; digestive tract morphogenesis; gonad morphogenesis; nuclear membrane organization and biogenesis. 29 alleles are reported. The phenotypes of these alleles are annotated with 20 unique terms, many of which group under: organ system; nuclear part; anatomical structure; embryonic/larval tracheal system; nucleus; gamete; nuclear membrane part; egg; peripheral nervous system; nervous system. It has one annotated transcript and one annotated polypeptide.
External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Lam: Lamin The structural gene for nuclear lamin. Translated as a prolamin of apparent molecular mass of 76-kd, which is quickly processed to a 74-kd species, presumably by proteolysis. The processed polypeptide is assembled into the nuclear envelope, where it becomes phosphorylated and again attains a mass of 76 kd (Smith, Gruenbaum, Berrios, and Fisher, 1987, J. Cell Biol. 105: 771-90).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25E6-25E6 Limits computationally determined from genome sequence between P{lacW}Hel25E^k11511 and P{lacW}l(2)k06502^k06502
Experimentally Determined Cytological Location
Cytogenetic map Notes References 25E5-25E6 (determined by in situ hybridisation) (Spradling et al., 1999) 25E4-25E6 25F1--2 (BDGP Project Members, 1994-1999) 25F1-25F2 (determined by in situ hybridisation) (Gruenbaum et al., 1988)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: Lam+ GluRIIA? (Lenz-Bohme et al., 1997) Overall orientation not stated: Lam? GluRIIA+ GluRIIB? (Petersen et al., 1997)
Gene Model & Products
Please see the GBrowse view of Dmel\Lam for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Lam-RA FBtr0079100 NM_057268 3144 622
Additional Transcript Data & Comments
Reported size (kB)	3.0, 2.8 (northern blot) (Gruenbaum et al., 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Lam-PA FBpp0078733 71.3 622 6.40 NP_476616 AAF52262
Additional Polypeptide Data & Comments
Reported size (kD)	622 (aa); 76 (kD observed); 71 (kD predicted) (Gruenbaum et al., 1988)
Comments	The 76 kD Lam polypeptide is post-translationally modified to give a 74 kD form. (Gruenbaum et al., 1988)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002525
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Intermediate filament, C-terminal (IPR001322) Intermediate filament protein (IPR001664) Filament (IPR016044)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263866 AA264919 AA391522 AA392198 AA439869 AA536273 AA539938 AA539968 AA697025 AA942106 AA949067 AA949619 AC009910 AE003610 AAF52262 AI238090 AI258219 AI514678 AI518515 AI531713 AI532792 AI534248 AI541863 AI542657 AI543526 AI544028 AI544054 AI546621 AQ025968 AQ034028 AX664513 CAD80036 BE976992 BF486626 BG639499 BI162086 BI170176 BI212173 BI227873 BI231720 BI237307 BI243854 BI353503 BI369860 BI370884 BI633014 BI640316 BP550516 BP550517 BT001506 AAN71261 CK657329 CL526005 CO178745 CO186701 CO190402 CO190907 CO262029 CO267646 CO271029 CO271133 CO272492 CO274242 CO274738 CO276659 CO277105 CO278530 CO282914 CO285134 CO289498 CO291814 CO293357 CO298008 CO302097 CO305205 CO307389 CO309884 CO310872 CO311681 CO315512 CO317550 CO319255 CO323422 CO329410 CO329746 CO330846 CO333638 CO335367 CS133217 EC060241 EC060356 EC062625 EC063136 EC066808 EC067367 EC071414 EC076486 EC077643 EC080867 EC081263 EC082687 EC087648 EC089708 EC194690 EC200386 EC201246 EC204341 EC204396 EC206333 EC208653 EC215190 EC215193 EC215463 EC216221 EC220545 EC222107 EC222967 EC223306 EC226452 EC227232 EC228341 EC229999 EC231466 EC232448 EC233636 EC234376 EC236647 EC236826 EC236831 EC237425 EC237788 EC238860 EC238987 EC242886 EC245511 EC246945 EC247875 EC264141 EC267687 G00464 X07278 CAA30259 X16275 CAA34351

UniProtKB/Swiss-Prot	P08928
UniProtKB/TrEMBL

Maps to	FBcl0297476 (FlyBase, 1992-)
Does NOT map to
Identified with	LD14549 LD38055 (BDGP Project Members, 2000-) LD10531 Dm4262 (Spradling et al., 1999) LD46046 (Smith et al., 2004) D1167 Dm0092 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References insertion site =P{lacW}Lam[k11511] 2L:5,542,255..5,542,262 evidence=experimental (BDGP Project Members, 1994-1999) point mutation Lam[Ari7] 2L:5,544,196..5,544,196 evidence=experimental na_change=C5544196T pr_change=Q208@\|Lam-PA reported_na_change=C622T reported_pr_change=Q208@ (Patterson et al., 2004) point mutation Lam[Ari3] 2L:5,544,394..5,544,394 evidence=experimental na_change=C5544394T pr_change=Q274@\|Lam-PA reported_na_change=C820T reported_pr_change=Q274@ (Patterson et al., 2004) rescue fragment Lam[+tLa] 2L:5,542,265..5,547,318 comment=Contains Lam sequences from 251bp upstream of the putative transcription start site through to approximately 0.9kb downstream of the polyadenylation site. evidence=experimental (Lenz-Bohme et al., 1997)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 6529
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference embryonic stage-adult stage (Riemer et al., 1995) embryonic stage-adult stage (Gruenbaum et al., 1988) embryonic stage-adult stage (Gruenbaum et al., 1988) embryonic stage (Riemer et al., 1995)
Marker for

Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference embryonic stage (Riemer et al., 1995) embryonic stage-adult stage (Riemer et al., 1995) embryonic stage-adult stage (Ashery-Padan et al., 1997) embryonic stage \| early (Harel et al., 1989) larval stage (Riemer et al., 1995) oogenesis stage (Ashery-Padan et al., 1997)
Marker for

Subcellular Localization
CV Term	nucleus nucleoplasm cytoplasm (Ashery-Padan et al., 1997) nucleus (Riemer et al., 1995) nuclear envelope (Harel et al., 1989)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002525 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0002525 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0002525
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Lam^A25 Lam^EY08333 semi-viable (with Df(2L)cl-h4) Lam⁸³ Lam^A25 Lam^Ari3 Lam^Ari7 Lam^K2 semi-viable \| recessive Lam⁸³ Lam^Ari3 Lam^Ari7 Lam^K2 lethal Lam⁰⁴⁶⁴³ Lam^CB03749 lethal \| recessive Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^25A Lam² Lam^ex91 Lam^ex98 lethal \| recessive \| third instar larval stage Lam⁰⁴⁶⁴³ lethal \| partially \| recessive Lam^P lethal \| pupal stage Lam¹⁴ Lam⁹ lethal \| pupal stage (with Df(2L)GpdhA) Lam¹⁴ lethal \| pharate adult stage Lam¹⁴ Lam⁹ lethal \| pharate adult stage (with Df(2L)GpdhA) Lam¹⁴ semi-lethal Lam^P semi-lethal \| pupal stage Lam^P semi-lethal \| pupal stage (with Lam^P) Lam¹⁴ semi-lethal \| pupal stage (with Lam¹⁴) Lam^P Other Phenotypes Allele wild-type Lam^+t4.2 Lam^+tLa visible \| adult stage Lam^A25 visible \| adult stage (with Df(2L)cl-h4) Lam^A25 locomotor behavior defective Lam¹⁴ Lam^P locomotor behavior defective (with Df(2L)GpdhA) Lam¹⁴ locomotor behavior defective (with Lam^P) Lam¹⁴ locomotor behavior defective (with Lam¹⁴) Lam^P locomotor behavior defective \| recessive Lam^P uncoordinated \| recessive Lam^P flight behavior defective Lam^P flightless \| recessive Lam^P cell growth defective Lam¹⁴ developmental rate defective Lam¹⁴ Lam⁹ developmental rate defective (with Df(2L)GpdhA) Lam^sz18 Sterility Allele sterile Lam^P sterile (with Lam^P) Lam¹⁴ sterile (with Lam¹⁴) Lam^P sterile \| female \| germ-line clone \| recessive Lam^unspecified female sterile \| recessive Lam^P male sterile \| recessive Lam^P fertile Lam^EY08333 Phenotype manifest in Allele embryonic tracheal system Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^a7 Lam^ex91 Lam^ex98 Lam^sz18 Lam^sz9 egg \| germ-line clone Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^a7 Lam^ex98 Lam^sz18 dorsal appendage \| germ-line clone Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^a7 dorsal appendage \| germ-line clone \| supernumerary Lam⁰²⁴⁵⁹ Lam⁰⁴⁶⁴³ Lam^a7 eye disc & nucleus \| ectopic \| somatic clone Lam⁰⁴⁶⁴³ egg chamber \| germ-line clone Lam⁰⁴⁶⁴³ Lam^a7 Lam^ex98 Lam^sz18 Lam^unspecified oocyte \|