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Gene Dmel\lab

General Information
Symbol	Dmel\lab	Species	D. melanogaster
Name	labial	Annotation symbol	CG1264
Feature type	protein_coding_gene	FlyBase ID	FBgn0002522
Created / Updated	2005-03-17/2005-03-17
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	84A1-84A1	Sequence location	3R:2,487,140..2,504,312 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene labial is referred to in FlyBase by the symbol lab (CG1264, FBgn0002522). It has the cytological map location 84A1. Its sequence location is 3R:2487140..2504312. Its molecular function is described as: specific RNA polymerase II transcription factor activity; transcription factor activity; sequence-specific DNA binding. It is involved in the biological processes: brain development; embryonic development; regulation of transcription from RNA polymerase II promoter; cell differentiation; midgut development; cell fate determination. 35 alleles are reported. The phenotypes of these alleles are annotated with 18 unique terms, many of which group under: organ system; adult segment; nervous system; embryonic nervous system; cephalopharyngeal skeleton; adult mesothoracic segment; embryonic head; adult brain; embryonic/larval hemocoel; commissure; peripheral nervous system; antennal segment. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84A1-84A1   Limits computationally determined from genome sequence between P{lacW}casj1C2 and P{PZ}lab01241
Experimentally Determined Cytological Location
Cytogenetic map Notes References 84A1-84A2 (determined by in situ hybridisation)   (Spradling et al., 1999) 84A1-84A2   (BDGP Project Members, 1994-1999) 84A1-84A2 (determined by in situ hybridisation)   (Diederich et al., 1989) 84A-84A (determined by in situ hybridisation)   (Mlodzik et al., 1988) 84A1-84A2 (determined by in situ hybridisation)   (Hoey et al., 1986)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: anon-84Ab+ anon-84Ac+ lab- (Diederich et al., 1989) Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\lab for information on other features
Comments on Gene Model
	An alternative transcript described in FBrf0047938 appears not to be encoded by the sequenced strain; a region of sequence polymorphism eliminates the alternative splice acceptor site.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) lab-RA FBtr0081696  NM_057265   2784   629
Additional Transcript Data & Comments
Reported size (kB)	3.0 (northern blot) (Mlodzik et al., 1988, Diederich et al., 1989) 2.8, 1.2, 0.8 (northern blot) (Hoey et al., 1986)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein lab-PA   FBpp0081194   67.5   629   7.83     NP_476613   AAF54098
Additional Polypeptide Data & Comments
Reported size (kD)	629 (aa); 68 (kD predicted) (Diederich et al., 1989)
Comments	dpp, and indirectly Ubx are required for lab expression in the midgut epithelium. (Immergluck et al., 1990)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0002522
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Homeobox (IPR001356) Homeobox protein, antennapedia type (IPR001827) Homeodomain-like (IPR009057) Homeodomain-related (IPR012287) TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • T02064 T02065
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AC001652   AE001572 AAD19811   AE003674 AAF54098   AY095089 AAM11417   BI375946   CO306026   CO314458   CO342269   EC059619   EC237974   EC248706   EC253545   G01161   M13570 AAA28610   X13103 CAA31495   X13104 CAB57786   X13104 CAB57787   X13105 CAB57787   X13106 CAB57787
UniProtKB/Swiss-Prot	P10105
UniProtKB/TrEMBL
Maps to	DS00004 (FlyBase, 1992-)
Does NOT map to
Identified with	RE63854 (BDGP Project Members, 2000-) Dm1875 (BDGP Project Members, 1994-1999, Spradling et al., 1999) D1164 Dm0075 (BDGP Project Members, 1994-1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References aberration junction Df(3R)MAP8.bk1 3R:2,509,407..2,510,425 linked_to=BamHI-EcoRI_rfrag evidence=experimental comment=Approximate position of aberration breakpoint inferred by curator from FBrf0049820. (Diederich et al., 1989) aberration junction Df(3R)MAP11.bk1 3R:2,493,265..2,501,004 linked_to=EcoRI-EcoRI_rfrag evidence=experimental comment=Aberration breakpoint localized to a restriction fragment; position of restriction fragment on reference sequence inferred by FlyBase curator. (Chouinard and Kaufman, 1991) aberration junction In(3R)lab[9].bk1 3R:2,493,265..2,501,004 linked_to=EcoRI-EcoRI_rfrag evidence=experimental comment=Approximate position of aberration breakpoint inferred by curator from FBrf0049820. (Diederich et al., 1989) aberration junction Df(3R)9A99.bk1 3R:2,478,403..2,482,846 comment=Position of breakpoint inferred by FlyBase curator; maps approximately to a 4.4kb EcoRI- HindIII fragment. (Mlodzik et al., 1988) deletion lab[14] 3R:2,500,999..2,503,313 comment=lab[14] is a small (<2kb) deletion which maps within a BamHI-EcoRI restriction fragment from Figure 1 of FBrf0049820. evidence=experimental linked_to=BamHI-EcoRI_rfrag (Diederich et al., 1989) insertion site lab[15] 3R:2,503,308..2,503,921 linked_to=PstI-BamHI_rfrag comment=Approximate position of insertion_site inferred by curator from Figure 1 of FBrf0049820. evidence=experimental (Diederich et al., 1989) protein binding site lab-protein_bind-1 3R:2,507,343..2,507,376 bound_moiety=brk-XP evidence=experimental (Bergman et al., 2004, Sivasankaran et al., 2000) protein binding site lab-protein_bind-2 3R:2,507,579..2,507,596 bound_moiety=brk-XP evidence=experimental (Sivasankaran et al., 2000, Bergman et al., 2004) protein binding site lab-protein_bind-3 3R:2,507,229..2,507,244 bound_moiety=brk-XP evidence=experimental (Sivasankaran et al., 2000, Bergman et al., 2004) regulatory region lab-reg_element-2 3R:2,506,698..2,507,916 evidence=experimental comment=midgut expression element linked_to=HindIII-EcoRV_rfrag (Tremml and Bienz, 1992, Chouinard and Kaufman, 1991) regulatory region lab-reg_element-4 3R:2,504,308..2,507,873 evidence=experimental comment=fragment extends from an EcoRI site to the transcription start (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-3 3R:2,504,308..2,507,873 linked_to=EcoRI-EcoRI_rfrag evidence=experimental comment=fragment extends from an EcoRI site to the transcription start (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-5 3R:2,507,354..2,507,592 evidence=experimental comment=fragment extends from an EcoRI site to the transcription start comment=dpp response element linked_to=NarI-BstXI_rfrag (Tremml and Bienz, 1992, Chouinard and Kaufman, 1991) regulatory region lab-reg_element-6 3R:2,504,308..2,506,732 evidence=experimental comment=procephalon expression maintenance element comment=fragment extends from an EcoRI site to the transcription start comment=fragment extends from an EcoRI site to the transcription start (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-9 3R:2,503,313..2,504,298 comment=imaginal disc repressor element evidence=experimental (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-12 3R:2,493,265..2,501,004 comment=dorsal ridge expression element evidence=experimental linked_to=EcoRI-EcoRI_rfrag (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-15 3R:2,493,265..2,495,265 comment=posterior midgut expression element evidence=experimental (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-16 3R:2,492,721..2,493,270 comment=late embryonic head expression element evidence=experimental linked_to=EcoRI-EcoRI_rfrag (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-1 3R:2,492,721..2,493,270 linked_to=EcoRI-EcoRI_rfrag comment=late embryonic head expression element evidence=experimental (Chouinard and Kaufman, 1991) regulatory region lab-reg_element-7 3R:2,506,698..2,507,916 linked_to=HindIII-EcoRV_rfrag comment=midgut expression element evidence=experimental (Tremml and Bienz, 1992) regulatory region lab-reg_element-8 3R:2,507,354..2,507,592 linked_to=NarI-BstXI_rfrag comment=dpp response element evidence=experimental (Tremml and Bienz, 1992)
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 8512
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 23029
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference  embryonic stage | stage 9-11  cephalic furrow | anterior to (Mlodzik et al., 1988)  embryonic stage | >=2-4 hr   (Diederich et al., 1989)  embryonic stage  mesothoracic segment | dorsal (Roder et al., 1992)  embryonic stage | 0-24 hours   (Hoey et al., 1986)  embryonic stage | 2-24 hours   (Hoey et al., 1986)  embryonic stage | extended germ band  parasegment 4,5 (Roder et al., 1992)  embryonic stage  mesothoracic segment | dorsal (Roder et al., 1992)  embryonic stage | contracted germ band  prothoracic segment | posterior <&> dorsal (Roder et al., 1992)  embryonic stage  metathoracic segment | dorsal (Roder et al., 1992)  larval stage,pupal stage   (Diederich et al., 1989)  embryonic stage,larval stage,pupal stage   (Mlodzik et al., 1988)  embryonic stage  posterior midgut primordium (Mlodzik et al., 1988)  embryonic stage  metathoracic segment | dorsal (Roder et al., 1992)  embryonic stage | extended germ band  parasegment 4..13 <of> midline (Roder et al., 1992)  embryonic stage  prothoracic segment | dorsal (Roder et al., 1992)  embryonic stage  abdominal segment 1..8 | dorsal (Roder et al., 1992)  embryonic stage  prothoracic segment | dorsal (Roder et al., 1992)  embryonic stage  hypopharyngeal lobe | anlagen (Mlodzik et al., 1988)  embryonic stage | 12-14 hours  central nervous system (Hoey et al., 1986)  embryonic stage  embryonic mandibular segment (Mlodzik et al., 1988)  embryonic stage | extended germ band  parasegment 4..13 <of> tracheal placode (Roder et al., 1992)  embryonic stage   (Roder et al., 1992)  embryonic stage | 0-24 hours   (Hoey et al., 1986)  embryonic stage  embryonic procephalic segment | anlagen (Mlodzik et al., 1988)  embryonic stage | 12-14 hours  embryonic/larval midgut (Hoey et al., 1986)  embryonic stage  abdominal segment 1..8 | dorsal (Roder et al., 1992)
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference  embryonic stage | stage 9-11  ectoderm & intercalary segment (Urbach and Technau, 2003)  embryonic stage | stage 12  embryonic/larval midgut | restricted (Diederich et al., 1989)  embryonic stage | stage 9-11  deuterocerebral neuroblast | subset (Urbach and Technau, 2003)  embryonic stage | mid-late  endoderm (Panganiban et al., 1990)  embryonic stage | stage 13-17  parasegment 7 <of> endoderm (Reuter et al., 1990)  embryonic stage  embryonic central nervous system | restricted (Diederich et al., 1989)  embryonic stage | mid-late  parasegment 7..12 <of> endoderm (Panganiban et al., 1990)  embryonic stage  midgut (Immergluck et al., 1990)  embryonic stage | stage 9-11  anterior midgut primordium (Diederich et al., 1989)  embryonic stage  midgut (Immergluck et al., 1990)  embryonic stage | mid-late  endoderm | anterior (Reuter et al., 1990)  embryonic stage  parasegment 1..7 <of> midgut (Immergluck et al., 1990)  embryonic stage | stage 13-14  larval sense organ | precursor (Diederich et al., 1989)  embryonic stage | stage 12  dorsal ridge (Diederich et al., 1989)  embryonic stage | stage 9-11  posterior midgut primordium (Diederich et al., 1989)  embryonic stage  embryonic/larval midgut (Immergluck et al., 1990)  embryonic stage  parasegment 8..12 <of> midgut (Immergluck et al., 1990)  embryonic stage | stage 9-11  procephalic segment | restricted (Diederich et al., 1989)  embryonic stage  midgut (Immergluck et al., 1990)  embryonic stage | stage 9-11  tritocerebral neuroblast (Urbach and Technau, 2003)
Marker for
Subcellular Localization
CV Term	nucleus (Immergluck et al., 1990)
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0002522 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0002522 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0002522 BDGP expression data • Show all BDGP original images, annotations and embryonic microarray data for lab FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for lab Stage(s) 4-6 Stage(s) 7-8 Stage(s) 9-10 Stage(s) 11-12 Stage(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable labPL00449 lethal | recessive lab01241 lab01241 lab1 lethal | recessive | conditional ts lab2 lethal | conditional ts lab2 lethal | conditional ts (with lab2) lab14 lethal | conditional ts (with lab14) lab2 lethal | embryonic stage | recessive lab14 lethal | larval stage | recessive lab14 lethal, with Scer\GAL4l(3)31-1-31-1 labScer\UAS.cMa lethal, with Scer\GAL469B labScer\UAS.cMa semi-lethal, with Scer\GAL4prd.RG1 labScer\UAS.cMa Other Phenotypes Allele wild-type labhs.PH visible (with lab2) lab14 visible (with lab14) lab2 visible | recessive lab2 Sterility Allele fertile labPL00449 Phenotype manifest in Allele crystal cell lab01241 lab4 embryonic head lab1 lab10 lab14 lab15 lab16 lab17 lab18 lab2 lab3 lab4 lab5 lab6 lab7 lab8 lab9 labAAA.hs labhs.PC larval salivary gland & embryo lab1 lab10 lab14 lab15 lab16 lab17 lab18 lab2 lab3 lab4 lab5 lab6 lab7 lab8 lab9 postorbital bristle (with lab2) lab14 postorbital bristle | supernumerary (with lab2) lab14 larval midgut (with lab2) lab14 adult midgut (with lab2) lab14 cephalopharyngeal skeleton lab14 labAAA.hs labhs.PC mouth hooks lab14 cuprophilic cell lab14 labhs.PH larval midgut & embryo lab14 lab2 tritocerebrum lab14 embryonic tritocerebrum lab14 tritocerebral neuromere (with lab9) lab2 longitudinal connective (with lab9) lab2 postorbital bristle (with lab14) lab2 postorbital bristle | supernumerary (with lab14) lab2 larval midgut (with lab14) lab2 adult midgut (with lab14) lab2 embryonic brain lab2 postorbital bristle lab2 larval midgut | temperature conditional lab2 tritocerebral neuromere (with lab2) lab9 longitudinal connective (with lab2) lab9 tritocerebral neuromere lab9 labunspecified longitudinal connective lab9 embryonic tritocerebrum, with Scer\GAL4sca-537.4 labScer\UAS.cMa wing vein, with Scer\GAL4sd-SG29.1 labScer\UAS.cMa wing margin, with Scer\GAL4sd-SG29.1 labScer\UAS.cMa arista labhs.PH midgut interstitial cell labhs.PH tritocerebral commissure labunspecified
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of lab Class Mutagen Stocks Known lesion lab01241 P-element activity 1 -- lab10 hypomorph ethyl methanesulfonate 1 -- lab11 loss of function diepoxybutane 1 -- lab12 hypomorph diepoxybutane 1 -- lab13 hypomorph diepoxybutane 1 -- lab14 amorph, loss of function X ray 2 Yes lab15 hypomorph X ray 1 Yes lab16 amorph, loss of function X ray 1 Yes lab17 hypomorph X ray 1 -- lab18 hypomorph X ray 1 -- lab1 hypomorph ethyl methanesulfonate 3 -- lab2 hypomorph X ray 1 -- lab3 hypomorph ethyl methanesulfonate 1 -- lab4 amorph, loss of function ethyl methanesulfonate 3 -- lab5 hypomorph ethyl methanesulfonate 1 -- lab6 hypomorph ethyl methanesulfonate 1 -- lab7 amorph, loss of function ethyl methanesulfonate 1 -- lab8 hypomorph ethyl methanesulfonate 1 -- lab9 amorph, loss of function X ray 1 Yes labMAP11 0 Yes labPL00449 P-element activity 1 -- labPL00757 piggyBac transposase 0 Yes labR4.2 2 Yes labunspecified 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of lab Class Mutagen Stocks Known lesion labAAA.hs in vitro construct | site directed mutagenesis 0 Yes labGD1477 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes labMtnA.T:Hsap\MYC in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes labScer\UAS.cMa in vitro construct | regulatory fusion 1 Yes labhs.PC in vitro construct | regulatory fusion 0 Yes labhs.PH in vitro construct | regulatory fusion 0 Yes labm2.2a in vitro construct | minigene 0 Yes labm2.3b in vitro construct | minigene 0 Yes labm2.4a in vitro construct | deletion in vitro construct | minigene 0 Yes labm2.4b in vitro construct | minigene 0 Yes labm2.5a in vitro construct | minigene 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)MAP2
Useful duplication	Dp(3;Y)77ab
Disrupted in	Df(3R)Antp-X1 (Hazelrigg and Kaufman, 1983) Df(3R)Antp-X2 (Hazelrigg and Kaufman, 1983) Df(3R)Dfd13 (Spradling et al., 1999, Hazelrigg and Kaufman, 1983) Df(3R)Dfdrv1 (Gonzalez-Reyes and Morata, 1991) Df(3R)MAP11 (Merrill et al., 1989, Diederich et al., 1989, Kaufman et al., 1990, Chouinard and Kaufman, 1991) Df(3R)MAP2 (Merrill et al., 1989) Df(3R)Scr (Nicholls and Gelbart, 1998, Spradling et al., 1999, Wakimoto et al., 1984, Merrill et al., 1989, Kaufman et al., 1990, BDGP Project Members, 1994-1999, Lewis et al., 1980, Kennison, 1995.6.21) In(3R)lab9 (Diederich et al., 1989, Kaufman et al., 1990, Randazzo et al., 1991) T(3;4)lab16 (Diederich et al., 1989)
Not disrupted in	Df(3R)Antp17 (Spradling et al., 1999,